FastQCFastQC Report
Fri 17 Jun 2016
SRR1527189_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527189_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6436
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC4767.395898073337477No Hit
TTGGACGGGGCCTGCCGGGTGAACCGCCCCGGTGCGTTCACTCCCGATAT2864.443753884400248No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA2774.303915475450591No Hit
TTGGACGGTCCTCGCTTAAGCTCGACAGCTCCCACATTAGATCCTCGCTC2233.4648850217526417No Hit
TTGGACGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACG2123.2939714108141707No Hit
TTGGACGGGGATTACCATGCAGGCGGAACACCTCGGCCAGTTGCGCGCCG1963.045369794903667No Hit
TTGGACGGGTGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGA1592.470478558110628No Hit
TTGGACGGGAATCAGCTTGTGGCGCGATACCGTCATTATGGAGCCACTGA1512.346177750155376No Hit
TTGGACGGGGGTTTGCGCTTGCGGTGGATCATTGCGAAATGCCAGGGCGC1462.2684897451833437No Hit
TTGGACGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTG1332.06650093225606No Hit
TTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGAT1161.8023617153511498No Hit
TTGGACGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGA1101.709136109384711No Hit
TTGGACGGGATGAGAAACTCGACACTACACGCTGGCGGAGGATCGGCAAG1071.6625233064014915No Hit
TTGGACGGGACTTTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA901.3983840894965818No Hit
TTGGACGGGGATCCTCTGATAGCCATATCAACATCATGCGCTTCTCGTCG881.3673088875077688No Hit
TTGGACGGGCAGGAGGATCCCTTGAATCTGGGAGGTGGAGGTTGCTGGGA871.3517712865133624No Hit
TTGGACGGGATCGTCCCAAAGGAGGAAGGCAAAGCGAATCCAAAGGTGCT801.2430080795525171No Hit
TTGGACGGGGATCCTGATCCCCACCACCAGGTAAAACGATCTTACGCTGC771.1963952765692978No Hit
TTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGA761.1808576755748912No Hit
TTGGACGGGATTGAAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA761.1808576755748912No Hit
TTGGACGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTT741.1497824735860782No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGACTCGTCTGGCAGCATTT580.9011808576755749No Hit
TTGGACGGGGACCTTCTCAGTACGCCCAGGTTGAAGTGGCATGTGCTTTT570.8856432566811684No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.8234928527035427No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.7458048477315102No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT430.6681168427594779No Hit
TTGGACGGGGCGCATCCGCCAGGCTGCGACGCTGCGTTCTGGCCAGTTCA410.6370416407706649No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT410.6370416407706649No Hit
TTGGACGGGATTTCCAAATTGATACATAAATATTTATAATCATTCACACC360.5593536357986327No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT350.5438160348042262No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT240.3729024238657551No Hit
TTGGACGGGGAATCAAAACCAAAATCGGTGGAAGCATGAGAATCACCATT240.3729024238657551No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT210.32628962088253577No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTT190.2952144188937228No Hit
TTGGACGGGTAAAGCAGTGATATCAACGCAAATTGCAGTGCTTTCGACGG160.2486016159105034No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG160.2486016159105034No Hit
TTGGACGGGAAGCTCTCGTTTGTAGAGTATCAATTCATGAGCAGCAGTAC150.23306401491609696No Hit
TTGGACGGGACTTTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.23306401491609696No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.2175264139216905No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT130.20198881292728404No Hit
TTGGACGGGATCTCTGTCCCGGCTATAGACGTCCTTGCCAGGGACGCTGC120.18645121193287756No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.18645121193287756No Hit
TTGGACGGGGCCTTAACACGCGCAGGTTATTTCTAACTTAAAAAAAAAAA110.1709136109384711No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTGGACGGGA110.1709136109384711No Hit
TTGGACGGGAAGCAGTAGTATCAACGCAGAATGCAATGCTTTGACGGGTT100.15537600994406464No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCCTTT90.13983840894965818No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGGAGCATTT90.13983840894965818No Hit
TTGGACGGGAAGCAGAGGTATCAATGCTTTGGACGGGGAAAAAAAAAAAA90.13983840894965818No Hit
TTGGACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1243008079552517No Hit
TTGGACGGGCGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGA80.1243008079552517No Hit
TTGGACGGGGCTTTGGACGGGGCCGTGGTATCAACTGCTTTGGACGGGAA80.1243008079552517No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGGCGCATTT80.1243008079552517No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1243008079552517No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.10876320696084525No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTGGCGCC70.10876320696084525No Hit
TTGGACGGGGAGTGCAGTGCTTTGGACGGGGGGCAAAAAAAAAAAAAAAA70.10876320696084525No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGCAGCATTT70.10876320696084525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAA650.044.0000045
GTAGATA207.283648E-444.023
CTTTTCC253.965133E-544.010
TGATGCT406.315531E-944.038
CGTTCAC406.315531E-944.035
CGCTGAT207.283648E-444.023
TTTTGGA207.283648E-444.044
TTCACTC406.315531E-944.037
GTTGATG406.315531E-944.036
GAGCGTG207.283648E-444.021
ATGGGCA207.283648E-444.037
TGGTTGA406.315531E-944.034
ACTCGTG207.283648E-444.042
GTGTTTT207.283648E-444.035
CAATGGG207.283648E-444.035
AACGCAG207.283648E-444.023
TGCACAT207.283648E-444.026
GGTGAAC406.315531E-944.018
GTCATTT207.283648E-444.011
GGCATTT207.283648E-444.040
Produced by FastQC (version 0.11.5)