##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527189_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6436
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33654443753884	33.0	31.0	34.0	31.0	34.0
2	32.759322560596644	34.0	31.0	34.0	31.0	34.0
3	32.796923555003104	34.0	31.0	34.0	31.0	34.0
4	36.38828464885022	37.0	37.0	37.0	35.0	37.0
5	35.37709757613425	37.0	35.0	37.0	33.0	37.0
6	36.10674331883157	37.0	36.0	37.0	35.0	37.0
7	36.41267868241144	37.0	37.0	37.0	35.0	37.0
8	36.628340584213795	37.0	37.0	37.0	35.0	37.0
9	38.67262274704785	39.0	39.0	39.0	38.0	39.0
10	38.01817899316345	39.0	38.0	39.0	35.0	39.0
11	37.95929148539466	39.0	38.0	39.0	35.0	39.0
12	36.62290242386575	38.0	35.0	39.0	33.0	39.0
13	35.61295835922933	38.0	35.0	39.0	30.0	39.0
14	36.32131758856433	39.0	35.0	40.0	30.0	40.0
15	36.89900559353636	39.0	35.0	40.0	32.0	40.0
16	37.19530764449969	39.0	35.0	40.0	34.0	40.0
17	37.13502175264139	39.0	35.0	40.0	33.0	40.0
18	37.01942200124301	38.0	35.0	40.0	33.0	40.0
19	36.899627097576136	39.0	35.0	40.0	33.0	40.0
20	36.35674331883157	38.0	35.0	40.0	31.0	40.0
21	36.79754505904288	38.0	35.0	40.0	33.0	40.0
22	36.63937228091983	38.0	35.0	40.0	32.0	40.0
23	36.502796768178996	38.0	35.0	40.0	32.0	40.0
24	36.21830329397141	38.0	35.0	40.0	31.0	40.0
25	36.43551895587321	38.0	35.0	40.0	32.0	40.0
26	36.56448104412679	38.0	35.0	40.0	33.0	40.0
27	36.162989434431324	38.0	35.0	40.0	32.0	40.0
28	36.247980111870724	38.0	35.0	40.0	32.0	40.0
29	36.367930391547546	38.0	35.0	40.0	32.0	40.0
30	36.58809819763828	38.0	35.0	40.0	33.0	40.0
31	36.45633934120572	38.0	35.0	40.0	33.0	40.0
32	35.65506525792418	37.0	35.0	40.0	31.0	40.0
33	35.502796768178996	36.0	35.0	40.0	30.0	40.0
34	35.547855811062774	36.0	35.0	40.0	31.0	40.0
35	35.6530453697949	36.0	35.0	40.0	31.0	40.0
36	34.370105655686764	35.0	34.0	40.0	26.0	40.0
37	32.68753884400249	35.0	32.0	40.0	15.0	40.0
38	32.35596643878185	35.0	33.0	39.0	12.0	40.0
39	32.14745183343692	35.0	32.0	39.0	12.0	40.0
40	32.060596643878185	35.0	32.0	39.0	9.0	40.0
41	31.855655686761963	35.0	31.0	39.0	11.0	40.0
42	31.79552517091361	35.0	31.0	39.0	10.0	40.0
43	31.305313859540085	35.0	31.0	38.0	9.0	40.0
44	31.299564947172158	35.0	31.0	39.0	10.0	40.0
45	31.460689869484153	35.0	31.0	39.0	10.0	40.0
46	31.06991920447483	35.0	31.0	39.0	8.0	40.0
47	30.587787445618396	35.0	29.0	38.0	8.0	40.0
48	30.836078309509013	35.0	28.0	38.0	10.0	40.0
49	31.509322560596644	35.0	29.0	37.0	15.0	40.0
50	30.888595400870106	35.0	28.0	37.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	3.0
21	13.0
22	18.0
23	28.0
24	51.0
25	59.0
26	82.0
27	171.0
28	332.0
29	174.0
30	262.0
31	224.0
32	211.0
33	292.0
34	591.0
35	740.0
36	782.0
37	885.0
38	1110.0
39	403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	95.57178371659415	0.3418272218769422	3.962088253573648	0.1243008079552517
8	97.63828464885022	0.2952144188937228	2.0198881292728403	0.04661280298321939
9	93.7538844002486	0.5748912367930391	4.226227470478558	1.4449968924798011
10	62.0261031696706	22.85581106277191	5.62461155997514	9.493474207582349
11	43.31883157240522	26.04101926662523	13.688626476072093	16.95152268489745
12	42.728402734617774	13.688626476072093	30.080795525170913	13.502175264139215
13	14.030453697949035	38.346799254195155	22.420758234928527	25.201988812927283
14	17.743940335612184	48.3064014916097	21.566190180236173	12.383467992541952
15	16.376631448104412	15.351149782473586	46.00683654443754	22.26538222498446
16	13.067122436295836	15.211311373523928	39.791796146674955	31.929770043505286
17	20.60285891858297	27.14418893722809	19.018023617153514	33.23492852703542
18	26.646985705407083	20.400870105655684	26.10316967060286	26.848974518334366
19	28.02983219390926	19.888129272840274	38.144810441267865	13.937228091982599
20	24.02113113735239	17.588564325668116	37.18147917961467	21.208825357364823
21	38.65755127408328	23.446239900559355	10.021752641392169	27.874456183965197
22	39.698570540708516	30.60907395898073	15.055935363579865	14.636420136730887
23	38.40894965817278	32.30267246737103	9.586699813548789	19.701678060907398
24	22.032318210068365	30.127408328154132	20.059042883778744	27.781230577998755
25	18.5052827843381	38.99937849596022	20.043505282784338	22.45183343691734
26	30.065257924176507	33.421379738968305	15.149160969546301	21.364201367308887
27	24.65817277812306	34.524549409571165	16.858297078931013	23.958980733374766
28	22.54505904288378	41.702921068986946	14.931634555624612	20.820385332504664
29	12.228091982597887	42.417650714729646	25.947793660658796	19.406463642013673
30	12.461155997513984	34.400248601615914	28.154133001864512	24.984462399005594
31	25.01553760099441	30.826600372902423	26.988812927284027	17.16904909881914
32	29.909881914232443	28.34058421379739	24.083281541330017	17.66625233064015
33	28.58918582970789	20.261031696706027	33.250466128029835	17.899316345556247
34	24.502796768178996	22.14108141702921	25.233064014916096	28.1230577998757
35	24.502796768178996	21.348663766314484	25.621504039776262	28.52703542573027
36	29.443753884400248	34.58669981354879	13.84400248601616	22.125543816034803
37	18.0236171535115	24.192044748290865	29.0863890615289	28.697949036668742
38	18.241143567433188	22.23430702299565	32.67557489123679	26.848974518334366
39	20.447482908638907	29.97203231821007	18.241143567433188	31.339341205717837
40	37.958359229334995	24.95338719701678	22.840273461777503	14.247980111870728
41	29.350528278433806	20.727159726538222	29.83219390926041	20.090118085767557
42	24.30080795525171	22.90242386575513	36.653200745804845	16.143567433188316
43	25.745804847731513	23.24425108763207	16.547545059042886	34.46239900559354
44	13.502175264139215	24.813548788067123	26.476072094468616	35.208203853325045
45	33.887507768800496	22.622747047855814	19.49968924798011	23.99005593536358
46	33.82535736482287	25.093225605966442	11.295835922933499	29.785581106277192
47	23.570540708514606	32.98632691112493	16.050341827221875	27.392790553138596
48	26.49160969546302	25.279676817899315	30.391547545059044	17.83716594157862
49	20.851460534493473	27.221876942200122	26.087632069608453	25.839030453697948
50	20.323182100683653	29.894344313238037	27.392790553138596	22.389683032939715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	7.0
12	13.0
13	11.5
14	10.0
15	10.5
16	11.0
17	84.0
18	157.0
19	113.0
20	69.0
21	55.0
22	41.0
23	31.5
24	22.0
25	43.0
26	64.0
27	62.5
28	61.0
29	115.0
30	169.0
31	109.5
32	50.0
33	39.0
34	28.0
35	109.0
36	190.0
37	136.0
38	82.0
39	62.5
40	43.0
41	49.0
42	55.0
43	70.5
44	86.0
45	223.0
46	360.0
47	284.5
48	209.0
49	198.5
50	188.0
51	275.5
52	363.0
53	899.0
54	1435.0
55	1043.5
56	652.0
57	533.5
58	415.0
59	435.5
60	456.0
61	378.0
62	300.0
63	206.5
64	113.0
65	206.0
66	299.0
67	350.0
68	401.0
69	221.0
70	41.0
71	32.0
72	23.0
73	17.0
74	11.0
75	10.5
76	10.0
77	8.0
78	6.0
79	3.0
80	0.0
81	0.0
82	0.0
83	0.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	6436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.867619639527657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.91028766455388	26.740211311373525
2	8.044856167723061	5.127408328154133
3	2.7303754266211606	2.610316967060286
4	1.4139444173573867	1.8023617153511498
5	0.8288639687957094	1.3206960845245495
6	0.29254022428083865	0.5593536357986327
7	0.19502681618722573	0.435052827843381
8	0.24378352023403219	0.6215040397762586
9	0.14627011214041932	0.41951522684897447
>10	1.023890784982935	7.411435674331883
>50	0.5363237445148707	12.678682411435673
>100	0.6338371526084837	40.27346177750155
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC	476	7.395898073337477	No Hit
TTGGACGGGGCCTGCCGGGTGAACCGCCCCGGTGCGTTCACTCCCGATAT	286	4.443753884400248	No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA	277	4.303915475450591	No Hit
TTGGACGGTCCTCGCTTAAGCTCGACAGCTCCCACATTAGATCCTCGCTC	223	3.4648850217526417	No Hit
TTGGACGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACG	212	3.2939714108141707	No Hit
TTGGACGGGGATTACCATGCAGGCGGAACACCTCGGCCAGTTGCGCGCCG	196	3.045369794903667	No Hit
TTGGACGGGTGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGA	159	2.470478558110628	No Hit
TTGGACGGGAATCAGCTTGTGGCGCGATACCGTCATTATGGAGCCACTGA	151	2.346177750155376	No Hit
TTGGACGGGGGTTTGCGCTTGCGGTGGATCATTGCGAAATGCCAGGGCGC	146	2.2684897451833437	No Hit
TTGGACGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTG	133	2.06650093225606	No Hit
TTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGAT	116	1.8023617153511498	No Hit
TTGGACGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGA	110	1.709136109384711	No Hit
TTGGACGGGATGAGAAACTCGACACTACACGCTGGCGGAGGATCGGCAAG	107	1.6625233064014915	No Hit
TTGGACGGGACTTTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	90	1.3983840894965818	No Hit
TTGGACGGGGATCCTCTGATAGCCATATCAACATCATGCGCTTCTCGTCG	88	1.3673088875077688	No Hit
TTGGACGGGCAGGAGGATCCCTTGAATCTGGGAGGTGGAGGTTGCTGGGA	87	1.3517712865133624	No Hit
TTGGACGGGATCGTCCCAAAGGAGGAAGGCAAAGCGAATCCAAAGGTGCT	80	1.2430080795525171	No Hit
TTGGACGGGGATCCTGATCCCCACCACCAGGTAAAACGATCTTACGCTGC	77	1.1963952765692978	No Hit
TTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGA	76	1.1808576755748912	No Hit
TTGGACGGGATTGAAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	1.1808576755748912	No Hit
TTGGACGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTT	74	1.1497824735860782	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGACTCGTCTGGCAGCATTT	58	0.9011808576755749	No Hit
TTGGACGGGGACCTTCTCAGTACGCCCAGGTTGAAGTGGCATGTGCTTTT	57	0.8856432566811684	No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53	0.8234928527035427	No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.7458048477315102	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTT	43	0.6681168427594779	No Hit
TTGGACGGGGCGCATCCGCCAGGCTGCGACGCTGCGTTCTGGCCAGTTCA	41	0.6370416407706649	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT	41	0.6370416407706649	No Hit
TTGGACGGGATTTCCAAATTGATACATAAATATTTATAATCATTCACACC	36	0.5593536357986327	No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT	35	0.5438160348042262	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTT	24	0.3729024238657551	No Hit
TTGGACGGGGAATCAAAACCAAAATCGGTGGAAGCATGAGAATCACCATT	24	0.3729024238657551	No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT	21	0.32628962088253577	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTT	19	0.2952144188937228	No Hit
TTGGACGGGTAAAGCAGTGATATCAACGCAAATTGCAGTGCTTTCGACGG	16	0.2486016159105034	No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG	16	0.2486016159105034	No Hit
TTGGACGGGAAGCTCTCGTTTGTAGAGTATCAATTCATGAGCAGCAGTAC	15	0.23306401491609696	No Hit
TTGGACGGGACTTTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.23306401491609696	No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.2175264139216905	No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT	13	0.20198881292728404	No Hit
TTGGACGGGATCTCTGTCCCGGCTATAGACGTCCTTGCCAGGGACGCTGC	12	0.18645121193287756	No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	12	0.18645121193287756	No Hit
TTGGACGGGGCCTTAACACGCGCAGGTTATTTCTAACTTAAAAAAAAAAA	11	0.1709136109384711	No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTGGACGGGA	11	0.1709136109384711	No Hit
TTGGACGGGAAGCAGTAGTATCAACGCAGAATGCAATGCTTTGACGGGTT	10	0.15537600994406464	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCCTTT	9	0.13983840894965818	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGGAGCATTT	9	0.13983840894965818	No Hit
TTGGACGGGAAGCAGAGGTATCAATGCTTTGGACGGGGAAAAAAAAAAAA	9	0.13983840894965818	No Hit
TTGGACGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.1243008079552517	No Hit
TTGGACGGGCGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGA	8	0.1243008079552517	No Hit
TTGGACGGGGCTTTGGACGGGGCCGTGGTATCAACTGCTTTGGACGGGAA	8	0.1243008079552517	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGGCGCATTT	8	0.1243008079552517	No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8	0.1243008079552517	No Hit
TTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.10876320696084525	No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTGGCGCC	7	0.10876320696084525	No Hit
TTGGACGGGGAGTGCAGTGCTTTGGACGGGGGGCAAAAAAAAAAAAAAAA	7	0.10876320696084525	No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGCAGCATTT	7	0.10876320696084525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.015537600994406464	0.0	0.0	0.0	0.0
12	0.04661280298321939	0.0	0.0	0.0	0.0
13	0.062150403977625855	0.0	0.0	0.0	0.0
14	0.13983840894965818	0.0	0.0	0.0	0.0
15	0.18645121193287756	0.0	0.0	0.0	0.0
16	0.23306401491609696	0.0	0.0	0.0	0.0
17	0.24860161591050342	0.0	0.0	0.0	0.0
18	0.2641392169049099	0.0	0.0	0.0	0.0
19	0.27967681789931637	0.0	0.0	0.0	0.0
20	0.3262896208825357	0.0	0.0	0.0	0.0
21	0.3262896208825357	0.0	0.0	0.0	0.0
22	0.4195152268489745	0.0	0.0	0.0	0.0
23	0.48166563082660035	0.0	0.0	0.0	0.0
24	0.5127408328154133	0.0	0.0	0.0	0.0
25	0.5282784338098198	0.0	0.0	0.0	0.0
26	0.5282784338098198	0.0	0.0	0.0	0.0
27	0.5748912367930391	0.0	0.0	0.0	0.0
28	0.6059664387818521	0.0	0.0	0.0	0.0
29	0.6215040397762586	0.0	0.0	0.0	0.0
30	0.6681168427594779	0.0	0.0	0.0	0.0
31	0.7458048477315102	0.0	0.0	0.0	0.0
32	0.7458048477315102	0.0	0.0	0.0	0.0
33	0.7768800497203232	0.0	0.0	0.0	0.0
34	0.7768800497203232	0.0	0.0	0.0	0.0
35	0.8079552517091361	0.0	0.0	0.0	0.0
36	0.8234928527035426	0.0	0.0	0.0	0.0
37	0.839030453697949	0.0	0.0	0.0	0.0
38	0.8545680546923555	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGAA	65	0.0	44.000004	5
GTAGATA	20	7.283648E-4	44.0	23
CTTTTCC	25	3.965133E-5	44.0	10
TGATGCT	40	6.315531E-9	44.0	38
CGTTCAC	40	6.315531E-9	44.0	35
CGCTGAT	20	7.283648E-4	44.0	23
TTTTGGA	20	7.283648E-4	44.0	44
TTCACTC	40	6.315531E-9	44.0	37
GTTGATG	40	6.315531E-9	44.0	36
GAGCGTG	20	7.283648E-4	44.0	21
ATGGGCA	20	7.283648E-4	44.0	37
TGGTTGA	40	6.315531E-9	44.0	34
ACTCGTG	20	7.283648E-4	44.0	42
GTGTTTT	20	7.283648E-4	44.0	35
CAATGGG	20	7.283648E-4	44.0	35
AACGCAG	20	7.283648E-4	44.0	23
TGCACAT	20	7.283648E-4	44.0	26
GGTGAAC	40	6.315531E-9	44.0	18
GTCATTT	20	7.283648E-4	44.0	11
GGCATTT	20	7.283648E-4	44.0	40
>>END_MODULE