FastQCFastQC Report
Fri 17 Jun 2016
SRR1527188_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527188_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7156
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4626.456120737842371No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA2863.9966461710452768No Hit
TTGGACGGGGATTACCATGCAGGCGGAACACCTCGGCCAGTTGCGCGCCGAT2763.8569032979318054No Hit
TTGGACGGGGCCTGCCGGGTGAACCGCCCCGGTGCGTTCACTCCCGATATTG2623.6612632755729457No Hit
TTGGACGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGA2112.9485746226942426No Hit
TTGGACGGTCCTCGCTTAAGCTCGACAGCTCCCACATTAGATCCTCGCTCGT1902.655114589155953No Hit
TTGGACGGGTGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGAAA1401.956400223588597No Hit
TTGGACGGGAATCAGCTTGTGGCGCGATACCGTCATTATGGAGCCACTGACC1371.9144773616545554No Hit
TTGGACGGGGGTTTGCGCTTGCGGTGGATCATTGCGAAATGCCAGGGCGCCA1361.9005030743432085No Hit
TTGGACGGGATGAGAAACTCGACACTACACGCTGGCGGAGGATCGGCAAGCT1331.8585802124091673No Hit
TTGGACGGGACTTTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1161.621017328116266No Hit
TTGGACGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCA1041.4533258803801008No Hit
TTGGACGGGCAGGAGGATCCCTTGAATCTGGGAGGTGGAGGTTGCTGGGATT821.145891559530464No Hit
TTGGACGGGGATCCTGATCCCCACCACCAGGTAAAACGATCTTACGCTGCTG811.1319172722191169No Hit
TTGGACGGGGATCCTCTGATAGCCATATCAACATCATGCGCTTCTCGTCGGT811.1319172722191169No Hit
TTGGACGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAG801.1179429849077696No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA761.0620458356623812No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGACTCGTCTGGCAGCATTTGA721.0061486864169928No Hit
TTGGACGGGATCGTCCCAAAGGAGGAAGGCAAAGCGAATCCAAAGGTGCTGG670.9362772498602571No Hit
TTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTA660.9223029625489101No Hit
TTGGACGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC610.8524315259921743No Hit
TTGGACGGGGACCTTCTCAGTACGCCCAGGTTGAAGTGGCATGTGCTTTTGT590.8244829513694802No Hit
TTGGACGGGATTGAAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.7685858021240917No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA530.7406372275013974No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA520.7266629401900504No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.7126886528787032No Hit
TTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACT490.6847400782560089No Hit
TTGGACGGGATTTCCAAATTGATACATAAATATTTATAATCATTCACACCTG460.6428172163219675No Hit
TTGGACGGGGGGAATACCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG370.5170486305198435No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT360.5030743432084964No Hit
TTGGACGGGGCGCATCCGCCAGGCTGCGACGCTGCGTTCTGGCCAGTTCACC360.5030743432084964No Hit
TTGGACGGGGCCTGCAGGGTGAACCGCCCCGGTGCGTTCACTCCCGATATTG350.4891000558971493No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT350.4891000558971493No Hit
TTGGACGGGGAATCAAAACCAAAATCGGTGGAAGCATGAGAATCACCATTTC320.4471771939631079No Hit
TTGGACGGTCCTCGCATAAGCTCGACAGCTCCCACATTAGATCCTCGCTCGT280.3912800447177194No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA260.3633314700950252No Hit
TTGGACGGGGGGAATACCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA250.34935718278367806No Hit
TTGGACGGGGATTACAATGCAGGCGGAACACCTCGGCCAGTTGCGCGCCGAT250.34935718278367806No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.3353828954723309No Hit
TTGGACGGGAAGCATAAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGA240.3353828954723309No Hit
TTGGACGGGGGGAATGCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG210.29346003353828953No Hit
TTGGACGGGCAGGAGAATCCCTTGAATCTGGGAGGTGGAGGTTGCTGGGATT200.2794857462269424No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA180.2515371716042482No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170.23756288429290107No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTA160.22358859698155395No Hit
TTGGACGGGGGAATTAATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCA160.22358859698155395No Hit
TTGGACGGGGGTTTGAGCTTGCGGTGGATCATTGCGAAATGCCAGGGCGCCA130.1816657350475126No Hit
TTGGACGGGTGTCATATAAAAGGTAGATAATATACAATGGGCATTTTGGAAA130.1816657350475126No Hit
TTGGACGGGTAAAGCAGTGATATCAACGCAAATTGCAGTGCTTTCGACGGGA120.16769144773616546No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTTAT120.16769144773616546No Hit
TTGGACGGGGGGAATACGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA120.16769144773616546No Hit
TTGGACGGGACTTTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.16769144773616546No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTA120.16769144773616546No Hit
TTGGACGGGATTGAAATGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.15371716042481834No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGA110.15371716042481834No Hit
TTGGACGGGCGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGAAA110.15371716042481834No Hit
TTGGACGGGAATCAGATTGTGGCGCGATACCGTCATTATGGAGCCACTGACC110.15371716042481834No Hit
TTGGACGGGTGCAGCGCTTTGGACGGGTGCAGTGCTTTGGACGGGGGGCAAA110.15371716042481834No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.15371716042481834No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTAT100.1397428731134712No Hit
TTGGACGGGGCTAATATGGGTGTGTGATGAATGCCTAGGGACAAGTTGCCGA100.1397428731134712No Hit
TTGGACGGGGGGAATACGTGGAGAAAGAAATGACTCGTCTGGCAGCATTTGA100.1397428731134712No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTGGACGGGAAG100.1397428731134712No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTA90.1257685858021241No Hit
TTGGACGGGCTTTTCACTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAG90.1257685858021241No Hit
TTGGACGGGGCCTGCGGGGTGAACCGCCCCGGTGCGTTCACTCCCGATATTG90.1257685858021241No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT90.1257685858021241No Hit
TTGGACGGGATCTCTGTCCCGGCTATAGACGTCCTTGCCAGGGACGCTGCAA80.11179429849077697No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTAT80.11179429849077697No Hit
TTGGACGGGACTTTGAACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11179429849077697No Hit
TTGGACGGGTGTCATGTAAAAGGTAGATAATATACAATGGGCATTTTGGAAA80.11179429849077697No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTGA80.11179429849077697No Hit
TTGGACGGGGATCCTATGATAGCCATATCAACATCATGCGCTTCTCGTCGGT80.11179429849077697No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGCAGCATTTGA80.11179429849077697No Hit
TTGGACAGGGACCTTCTCAGTACGCCCAGGTTGAAGTGGCATGTGCTTTTGT80.11179429849077697No Hit
TTGGACGGGAAGCATGAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGA80.11179429849077697No Hit
TTGGACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA80.11179429849077697No Hit
TTGGACGGGAAGCTCTCGTTTGTAGAGTATCAATTCATGAGCAGCAGTACCA80.11179429849077697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCC205.8980443E-446.026
CGACAGC205.8980443E-446.023
CTACACG205.8980443E-446.025
CGACACT205.8980443E-446.020
ATCCTCG205.8980443E-446.041
TAGCGCC550.046.044
TTTTGCT600.046.029
GGGTTTG301.6138347E-646.09
GGATGAG205.8980443E-446.08
ACACGCT205.8980443E-446.027
TCCTTCG850.046.016
GTTTGCG205.8980443E-446.011
CACATTA205.8980443E-446.033
ACTCGAC205.8980443E-446.017
ACGCTGG205.8980443E-446.029
TCCCACA205.8980443E-446.030
CATTGCG301.6138347E-646.030
CGGAGGA205.8980443E-446.036
AGCTCGA205.8980443E-446.019
TCGATTC600.046.020
Produced by FastQC (version 0.11.5)