FastQCFastQC Report
Fri 17 Jun 2016
SRR1527187_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527187_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7014
Sequences flagged as poor quality0
Sequence length52
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG4226.016538351867693No Hit
TTGGACGGGGCCTGCCGGGTGAACCGCCCCGGTGCGTTCACTCCCGATATTG2773.949244368406045No Hit
TTGGACGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA2463.507271171941831No Hit
TTGGACGGGGATTACCATGCAGGCGGAACACCTCGGCCAGTTGCGCGCCGAT2433.4644995722840037No Hit
TTGGACGGTCCTCGCTTAAGCTCGACAGCTCCCACATTAGATCCTCGCTCGT2313.293413173652695No Hit
TTGGACGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGA2303.2791559737667524No Hit
TTGGACGGGTGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGAAA1762.5092671799258626No Hit
TTGGACGGGGGTTTGCGCTTGCGGTGGATCATTGCGAAATGCCAGGGCGCCA1622.309666381522669No Hit
TTGGACGGGAATCAGCTTGTGGCGCGATACCGTCATTATGGAGCCACTGACC1381.9674935842600514No Hit
TTGGACGGGATGAGAAACTCGACACTACACGCTGGCGGAGGATCGGCAAGCT1091.5540347875677218No Hit
TTGGACGGGCAGGAGGATCCCTTGAATCTGGGAGGTGGAGGTTGCTGGGATT1031.4684915882520673No Hit
TTGGACGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGCA981.3972055888223553No Hit
TTGGACGGGACTTTGGACGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA961.3686911890504705No Hit
TTGGACGGGGATCCTCTGATAGCCATATCAACATCATGCGCTTCTCGTCGGT861.2261191901910464No Hit
TTGGACGGGATTGAAGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA731.0407755916737953No Hit
TTGGACGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTGC640.9124607927003138No Hit
TTGGACGGGGACCTTCTCAGTACGCCCAGGTTGAAGTGGCATGTGCTTTTGT630.8982035928143712No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGACTCGTCTGGCAGCATTTGA620.8839463929284289No Hit
TTGGACGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.8839463929284289No Hit
TTGGACGGGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGAAG610.8696891930424864No Hit
TTGGACGGGGATCCTGATCCCCACCACCAGGTAAAACGATCTTACGCTGCTG610.8696891930424864No Hit
TTGGACTTGGTGGCAAGTCTGCCGCTGATAAAGGAAAGGATACTCGTGATTA600.8554319931565441No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA590.8411747932706017No Hit
TTGGACGGGATCGTCCCAAAGGAGGAAGGCAAAGCGAATCCAAAGGTGCTGG560.7984031936127743No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA550.784145993726832No Hit
TTGGACGGGATTTCCAAATTGATACATAAATATTTATAATCATTCACACCTG510.7271171941830624No Hit
TTGGACGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.6273167949814656No Hit
TTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTCGCCAAATGACGACT420.5988023952095809No Hit
TTGGACGGGGCGCATCCGCCAGGCTGCGACGCTGCGTTCTGGCCAGTTCACC420.5988023952095809No Hit
TTGGACGGGGAATCAAAACCAAAATCGGTGGAAGCATGAGAATCACCATTTC330.4704875962360992No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT270.3849443969204448No Hit
TTGGACGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCATTGTAGCGCCGG210.29940119760479045No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT210.29940119760479045No Hit
TTGGACTATCTTATGCATGGTTTAGTCCTCTGGCTTGGATAACAGTACCCAT210.29940119760479045No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGA170.24237239806102082No Hit
TTGGACGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTGGACGGGAAG170.24237239806102082No Hit
TTGGACGGGTGCAGCGCTTTGGACGGGTGCAGTGCTTTGGACGGGGGGCAAA160.22811519817507844No Hit
TTGGACGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA160.22811519817507844No Hit
TTGGACGGGCGTCATTTAAAAGGTAGATAATATACAATGGGCATTTTGGAAA150.21385799828913601No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTA150.21385799828913601No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTA150.21385799828913601No Hit
TTGGACGGGGGGAATTCCTTGGGCAACATTAACTGGTTGATGCTGAAAAACA140.19960079840319359No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA130.1853435985172512No Hit
TTGGACGGGGGGAATTCCTTGGATTCTGTTTTGCTACCCGTTGTAGCGCCGG130.1853435985172512No Hit
TTGGACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1710863986313088No Hit
TTGGACGGGATCTCTGTCCCGGCTATAGACGTCCTTGCCAGGGACGCTGCAA110.1568291987453664No Hit
TTGGACGGTCCTCGCTTAAGGTCGACAGCTCCCACATTAGATCCTCGCTCGT110.1568291987453664No Hit
TTGGACGGGACTTTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1568291987453664No Hit
TTGGACGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTATTCCGCCACGGA110.1568291987453664No Hit
TTGGACGGGGCCTGCCGGGTGAACCGCCCCGGTGCGTTCGCTCCCGATATTG100.142571998859424No Hit
TTGGACGGGGATTACCATGCGGGCGGAACACCTCGGCCAGTTGCGCGCCGAT100.142571998859424No Hit
TTGGACGGGTAAAGCAGTGATATCAACGCAAATTGCAGTGCTTTCGACGGGA100.142571998859424No Hit
TTGGACGGGAAGCTCTCGTTTGTAGAGTATCAATTCATGAGCAGCAGTACCA100.142571998859424No Hit
TTGGACGGGGGGAATTCCTTGGGCAACATTAACTGGTTGGTGCTGAAAAACA90.1283147989734816No Hit
TTGGACGGGTGTCATTTAAAGGGTAGATAATATACAATGAGCATTTTGGAAA90.1283147989734816No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTTAT90.1283147989734816No Hit
TTGGACGGTCCTCGCTTAAGGTCGACAGCTCCCACATTAAATCCTCGCTCGT90.1283147989734816No Hit
TTGGACGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGCTGGCAGCATTTTA90.1283147989734816No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT90.1283147989734816No Hit
TTGGACGGGCTTTTCCCTTTCTGCCACCGCCATGCCGTCCAGACTGAGGAAG90.1283147989734816No Hit
TTGGACGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTAT80.11405759908753922No Hit
TTGGACGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTGTTCCGCCACGGA80.11405759908753922No Hit
TTGGACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.11405759908753922No Hit
TTGGACGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTGGCGCCGG80.11405759908753922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCC358.390634E-846.00000426
CGACAGC358.390634E-846.00000423
GTGTTTT358.390634E-846.00000435
TCCTTCG700.046.00000416
TGCACAT358.390634E-846.00000426
CACATTT358.390634E-846.00000428
AGCTCGA358.390634E-846.00000419
AGCTCCC358.390634E-846.00000427
TCGACAG358.390634E-846.00000422
TAAGCTC358.390634E-846.00000417
ATTAGGC358.390634E-846.00000414
GACAGCT358.390634E-846.00000424
CGGGGGG1650.046.0000046
GGTCCTC358.390634E-846.0000047
TTCCGCC358.390634E-846.00000441
CCACGGA358.390634E-846.00000446
CCGCCAC358.390634E-846.00000443
TGTTTTT358.390634E-846.00000436
TTTTCCG358.390634E-846.00000439
GTTTTTC358.390634E-846.00000437
Produced by FastQC (version 0.11.5)