FastQCFastQC Report
Fri 17 Jun 2016
SRR1527186_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527186_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2386034
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC148550.6225812373168195No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG89900.37677585482855647No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT87860.3682261023941822No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGA87200.36546000601835515No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT71920.3014206838628452No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC60040.2516309490979592No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG51460.2156716962122082No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC50380.21114535668812767No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA50230.21051669842089427No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC48840.20469113181119802No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC47670.19978759732677742No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG41990.17598240427420564No Hit
TCACTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG38590.1617328168835817No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT38120.1597630209795837No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC34690.14538770193551306No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT31840.1334431948580783No Hit
TCACTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT29260.12263027266166367No Hit
TCACTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC29070.12183397218983467No Hit
TCACTTGGGACATTTTGTTTTTGAGACTGTATTCCCTAGCATATATCTCC28180.1181039331375831No Hit
TCACTTGGGAGGACGTGTTTATATTGAGTCACATTCAAGACCTGGGCTGG27560.11550547896635169No Hit
TCACTTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC26450.11085340778882447No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG25730.1078358481061041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCACG207.858186E-444.00000434
CGACGTA207.858186E-444.00000434
TAGCGAA207.858186E-444.00000432
CGTTAAC207.858186E-444.00000439
ACGATAG207.858186E-444.00000433
AACGATA207.858186E-444.00000432
GAACGTA207.858186E-444.00000423
CGTAATC207.858186E-444.00000418
TACGTTA207.858186E-444.00000413
CGCGATT254.4442844E-544.044
CGCGTTT302.5288664E-644.032
TATACGC254.4442844E-544.042
CGATAGT302.5288664E-644.019
CGATAGG254.4442844E-544.034
TACCGTA1000.044.040
TACCGAC351.4469697E-744.041
TTGGGAT283950.042.0475465
CGTATAC1050.041.90476222
TTGGGAC165650.041.3039555
TCACTTG2535350.041.2874761
Produced by FastQC (version 0.11.5)