FastQCFastQC Report
Fri 17 Jun 2016
SRR1527185_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527185_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2557721
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC144240.5639395383624719No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG86160.3368623864760856No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT82360.3220054102851718No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG75280.29432451780315366No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT68270.2669173064614944No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC56900.22246366980604998No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT50240.19642486416618543No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG49400.1931406904818782No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA46440.18156788797527174No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT45080.17625065439115525No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC43980.1719499507569434No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG40870.1597906886638535No Hit
TCACTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36500.14270516604430272No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT34910.1364886944275783No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT34190.1336736884124578No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT30180.1179956688004673No Hit
TCACTTGGGACATTTTGTTTTTGAGACTGTATTCCCTAGCATATATCTCCTA27680.10822134235907668No Hit
TCACTTGGGAGGACGTGTTTATATTGAGTCACATTCAAGACCTGGGCTGGCC27040.1057191147900807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATAC351.0202348E-746.00000434
CGATGTA351.0202348E-746.00000440
AAACGAC405.6152203E-946.036
ACGATAC206.312913E-446.045
CGTACTA206.312913E-446.023
CGATAGT253.4183395E-546.019
ACGAATT253.4183395E-546.028
TTGGGAT289300.043.7182855
TTGGGAC175500.043.208555
TCACTTG2727250.043.0137331
CTTGGGA996600.042.9490284
CACTTGG2721700.042.9256742
ACTTGGG2671950.042.7539443
TTGGGAG351300.042.6609735
CTTGGGG1353600.042.5132984
TAAGGCG650.042.4615432
TGGGATA33000.042.1666646
TGGGATT114600.042.1265266
TTGGGGC294200.042.075465
TTGGGGG492600.041.7978065