##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527185_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2557721
Sequences flagged as poor quality	0
Sequence length	52
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.61382574565404	34.0	31.0	34.0	31.0	34.0
2	32.8153410790309	34.0	31.0	34.0	31.0	34.0
3	32.94644529250845	34.0	31.0	34.0	31.0	34.0
4	36.462843679979166	37.0	37.0	37.0	35.0	37.0
5	36.32118475783715	37.0	37.0	37.0	35.0	37.0
6	36.50340127011508	37.0	37.0	37.0	35.0	37.0
7	36.5512501168032	37.0	37.0	37.0	35.0	37.0
8	36.64123334796876	37.0	37.0	37.0	35.0	37.0
9	38.720593841157815	39.0	39.0	39.0	39.0	39.0
10	38.1536492838742	39.0	39.0	39.0	37.0	39.0
11	37.926268736895075	39.0	38.0	39.0	35.0	39.0
12	37.52865148309765	39.0	37.0	39.0	35.0	39.0
13	37.465318930407186	39.0	37.0	39.0	35.0	39.0
14	38.324798521809065	40.0	38.0	40.0	35.0	40.0
15	38.207759173107625	40.0	38.0	40.0	35.0	40.0
16	33.07477633408804	40.0	35.0	40.0	3.0	40.0
17	34.7940346112809	40.0	35.0	40.0	17.0	40.0
18	36.875169340205595	40.0	35.0	40.0	32.0	40.0
19	37.7198580298633	40.0	37.0	40.0	34.0	40.0
20	37.98538660002401	40.0	38.0	40.0	34.0	40.0
21	38.18118434340571	40.0	38.0	40.0	34.0	40.0
22	38.2423884387703	40.0	38.0	40.0	34.0	40.0
23	38.22410888443267	40.0	38.0	40.0	35.0	40.0
24	38.203971035151994	40.0	38.0	40.0	35.0	40.0
25	38.16003661071712	40.0	38.0	40.0	35.0	40.0
26	38.05742494978929	40.0	38.0	40.0	34.0	40.0
27	37.95711572919799	40.0	38.0	40.0	34.0	40.0
28	37.86237787467828	40.0	37.0	40.0	34.0	40.0
29	37.66267939310034	40.0	37.0	40.0	34.0	40.0
30	37.65347940608065	40.0	37.0	40.0	33.0	40.0
31	37.61141735161888	40.0	37.0	40.0	33.0	40.0
32	37.51044660461403	40.0	36.0	40.0	33.0	40.0
33	37.147944986963005	40.0	36.0	40.0	33.0	40.0
34	37.078487059378254	40.0	36.0	40.0	32.0	40.0
35	37.020958501728686	40.0	35.0	40.0	32.0	40.0
36	37.03213368463566	39.0	35.0	40.0	32.0	40.0
37	36.72438901662848	39.0	35.0	40.0	31.0	40.0
38	36.73814735852738	39.0	35.0	40.0	31.0	40.0
39	36.37414166752355	39.0	35.0	40.0	31.0	40.0
40	36.365528922036454	39.0	35.0	40.0	30.0	40.0
41	36.33487976210071	39.0	35.0	40.0	31.0	40.0
42	36.28169882485228	38.0	35.0	40.0	31.0	40.0
43	36.2108275296641	38.0	35.0	40.0	31.0	40.0
44	36.41788725197158	39.0	35.0	40.0	31.0	40.0
45	36.45343843210421	39.0	35.0	40.0	31.0	40.0
46	36.401489059987384	39.0	35.0	40.0	31.0	40.0
47	36.06645642742113	38.0	35.0	40.0	31.0	40.0
48	36.03995431870794	38.0	35.0	40.0	31.0	40.0
49	35.97540857661958	38.0	35.0	40.0	31.0	40.0
50	35.452143920310306	37.0	35.0	40.0	29.0	40.0
51	35.53469201683843	37.0	35.0	40.0	30.0	40.0
52	35.20841874465589	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	7.0
14	20.0
15	10.0
16	43.0
17	89.0
18	248.0
19	442.0
20	815.0
21	1434.0
22	2445.0
23	3860.0
24	5779.0
25	8583.0
26	11724.0
27	16261.0
28	22380.0
29	29520.0
30	38225.0
31	48802.0
32	64925.0
33	98204.0
34	144579.0
35	206602.0
36	264411.0
37	483322.0
38	668459.0
39	436532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.69058392217134	0.22946208753808567	0.05547907688133303	0.024474913409242054
8	99.57329200487465	0.31242657037260907	0.07045334498954343	0.043828079763195436
9	97.5999727882752	1.3229746324951002	0.4134540084708223	0.6635985707588905
10	49.86747968210762	37.42847636626512	5.105404381478667	7.598639570148581
11	34.20361329480424	22.199098337934434	23.962308633349767	19.634979733911557
12	30.346624983725746	15.516391349955683	27.82918856278695	26.30779510353162
13	23.830159739862168	17.228266882900833	30.0554673476896	28.8861060295474
14	24.56597103437005	18.486613668965457	30.877097228352895	26.070318068311593
15	26.696109544395185	18.79759363902474	28.823042075347544	25.683254741232524
16	29.313595970788057	27.495258474243283	22.728319468777087	20.462826086191573
17	31.63374738683383	20.589735940706593	23.085043286582078	24.691473385877504
18	29.323643978369805	19.82518812646102	24.849621987699205	26.001545907469968
19	29.18797632736331	20.161698637185214	25.107664205751917	25.542660829699564
20	26.866495602921507	21.903757290181378	25.972770290426517	25.2569768164706
21	28.912144835187263	20.368288800850447	26.56380426168452	24.15576210227777
22	28.674589605355706	19.297452693237457	26.021563728021942	26.0063939733849
23	28.42186462088711	21.022777699365957	24.990294093843698	25.565063585903232
24	28.4871571215156	21.201765165160705	24.96718758613625	25.343890127187446
25	27.28522774767068	20.24861194790206	24.531760891825183	27.93439941260208
26	27.291717900427763	19.81643032996953	25.14871637680576	27.743135392796948
27	26.822706620464075	18.58732832861755	24.508771676035032	30.08119337488334
28	28.48528045083885	22.057996161426523	22.45139325203961	27.005330135695022
29	30.243486291116195	21.088969438027057	23.757047778080565	24.910496492776186
30	29.336311505437852	20.526711083812504	23.447279824500015	26.689697586249633
31	31.262792149730167	20.937701962020093	23.228569496047456	24.570936392202277
32	32.51382773961664	20.36015656125121	23.376005436089393	23.750010263042764
33	31.641840529127297	20.657296085069483	24.810602876545175	22.890260509258045
34	28.33737534312773	22.670338164326758	25.610494655202814	23.381791837342696
35	27.162384012955282	25.73533235251226	25.408635265535217	21.693648368997245
36	28.188180024326343	25.352374242538573	23.021040997043855	23.438404736091233
37	28.96469161413618	26.02074268460086	21.950713154405815	23.063852546857145
38	30.749014454664913	25.10801608150381	21.817078563299123	22.32589090053215
39	29.783389196867056	23.44325280200616	21.78583981599244	24.987518185134345
40	28.07604895139071	23.65637221573424	22.52309771081365	25.744481122061398
41	24.240094990814086	24.26941797013826	23.994055645631406	27.49643139341625
42	24.457554205482147	22.399081056925287	23.80943816780642	29.33392656978615
43	25.577457431830915	21.56951442319158	25.093393689147486	27.759634455830014
44	26.008739811730834	20.55943552873828	25.4622376717398	27.969586987791082
45	26.946097717460194	21.874317018939905	25.092729034949475	26.08685622865043
46	26.917713073474392	22.494595774910557	24.328103026092368	26.259588125522683
47	26.14804351217353	22.67295768381305	24.87010897592036	26.308889828093058
48	27.16171935875727	21.634181366927823	25.335484206447852	25.868615067867058
49	26.966584705681345	21.831270885292025	25.41207582844259	25.79006858058404
50	25.19211438620553	23.15788156722332	24.844852116395806	26.80515193017534
51	24.28353209751963	23.227474771486023	25.40542928646244	27.08356384453191
52	26.116765667561083	21.86407352482933	25.776423620871864	26.242737186737724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	26.0
6	50.0
7	32.0
8	14.0
9	564.5
10	1115.0
11	2171.5
12	3228.0
13	4690.5
14	5775.5
15	5398.0
16	5330.0
17	5262.0
18	5621.0
19	5980.0
20	5878.0
21	5776.0
22	7883.5
23	9991.0
24	10229.0
25	10467.0
26	13491.5
27	16516.0
28	16957.0
29	17398.0
30	21448.0
31	25498.0
32	28454.0
33	31410.0
34	32496.0
35	33582.0
36	40907.5
37	48233.0
38	52192.0
39	60289.0
40	64427.0
41	66966.0
42	69505.0
43	76160.5
44	82816.0
45	89017.0
46	95218.0
47	97328.0
48	99438.0
49	110326.5
50	121215.0
51	134825.5
52	148436.0
53	162639.0
54	176842.0
55	198303.0
56	219764.0
57	238514.5
58	257265.0
59	245142.0
60	233019.0
61	217660.0
62	202301.0
63	175349.0
64	131093.0
65	113789.0
66	94771.5
67	75754.0
68	67667.5
69	59581.0
70	50889.0
71	42197.0
72	39680.5
73	37164.0
74	27139.5
75	17115.0
76	12532.5
77	7950.0
78	5035.5
79	2121.0
80	1571.0
81	1021.0
82	566.0
83	111.0
84	67.5
85	24.0
86	16.5
87	9.0
88	6.5
89	5.5
90	7.0
91	7.0
92	7.0
93	3.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2557721.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.068099092294371
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.87479670229246	5.5568868475572515
2	10.39151483679011	1.6767954284453959
3	4.514262660309943	1.0926455541607507
4	2.3146695187427784	0.7469993217252024
5	1.3450684899003174	0.5426072931219256
6	0.8092275191366393	0.3917356687563568
7	0.5492384080448671	0.3101916930979999
8	0.4027246816035672	0.25993781104722247
9	0.31101475991462424	0.22583681119415985
>10	4.006628562152943	8.708642361492558
>50	3.157425340780516	18.616235944914433
>100	3.1299450847061614	44.785660199588214
>500	0.13347552950368508	7.127815259379193
>1k	0.056082155253649194	7.408531609188997
>5k	0.0033649293152189516	1.8968262573141148
>10k+	5.608215525364919E-4	0.652651939016216
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14424	0.5639395383624719	No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8616	0.3368623864760856	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8236	0.3220054102851718	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG	7528	0.29432451780315366	No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6827	0.2669173064614944	No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5690	0.22246366980604998	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	5024	0.19642486416618543	No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4940	0.1931406904818782	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4644	0.18156788797527174	No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4508	0.17625065439115525	No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4398	0.1719499507569434	No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4087	0.1597906886638535	No Hit
TCACTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3650	0.14270516604430272	No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3491	0.1364886944275783	No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3419	0.1336736884124578	No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	3018	0.1179956688004673	No Hit
TCACTTGGGACATTTTGTTTTTGAGACTGTATTCCCTAGCATATATCTCCTA	2768	0.10822134235907668	No Hit
TCACTTGGGAGGACGTGTTTATATTGAGTCACATTCAAGACCTGGGCTGGCC	2704	0.1057191147900807	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.5638922306224955E-4	0.0	0.0	0.0	0.0
9	2.736811403589367E-4	0.0	0.0	0.0	0.0
10	0.0057864012533032335	0.0	0.0	0.0	0.0
11	0.012667527068042214	0.0	0.0	0.0	0.0
12	0.017202814536847452	0.0	0.0	0.0	0.0
13	0.02173810200565269	0.0	0.0	0.0	0.0
14	0.036868759336925336	0.0	0.0	0.0	0.0
15	0.04461002587850669	0.0	0.0	0.0	0.0
16	0.060288045490497204	0.0	0.0	0.0	0.0
17	0.06720826861100175	0.0	0.0	0.0	0.0
18	0.07346383753349173	0.0	0.0	0.0	0.0
19	0.08249531516533665	0.0	0.0	0.0	0.0
20	0.09148769549141599	0.0	0.0	0.0	0.0
21	0.10415522255945821	0.0	0.0	0.0	0.0
22	0.11260024060481968	0.0	0.0	0.0	0.0
23	0.12127984248477452	0.0	0.0	0.0	0.0
24	0.1352375806430803	0.0	0.0	0.0	0.0
25	0.1407503007560246	0.0	0.0	0.0	0.0
26	0.14868705382643377	0.0	0.0	0.0	0.0
27	0.15595915269882837	0.0	0.0	0.0	0.0
28	0.17429578910287713	0.0	0.0	0.0	0.0
29	0.18082503916572604	0.0	0.0	0.0	0.0
30	0.19110763058206895	0.0	0.0	0.0	0.0
31	0.19951355132166487	0.0	0.0	0.0	0.0
32	0.2087405154823376	0.0	0.0	0.0	0.0
33	0.21702914430463682	0.0	0.0	0.0	0.0
34	0.22594333001918504	0.0	0.0	0.0	0.0
35	0.25432797400498336	0.0	0.0	0.0	0.0
36	0.2667609172384322	0.0	0.0	0.0	0.0
37	0.27770816285278965	0.0	0.0	0.0	0.0
38	0.2868569324019313	0.0	0.0	0.0	0.0
39	0.2971004265125086	0.0	0.0	0.0	0.0
40	0.3064837798962436	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAC	35	1.0202348E-7	46.000004	34
CGATGTA	35	1.0202348E-7	46.000004	40
AAACGAC	40	5.6152203E-9	46.0	36
ACGATAC	20	6.312913E-4	46.0	45
CGTACTA	20	6.312913E-4	46.0	23
CGATAGT	25	3.4183395E-5	46.0	19
ACGAATT	25	3.4183395E-5	46.0	28
TTGGGAT	28930	0.0	43.718285	5
TTGGGAC	17550	0.0	43.20855	5
TCACTTG	272725	0.0	43.013733	1
CTTGGGA	99660	0.0	42.949028	4
CACTTGG	272170	0.0	42.925674	2
ACTTGGG	267195	0.0	42.753944	3
TTGGGAG	35130	0.0	42.660973	5
CTTGGGG	135360	0.0	42.513298	4
TAAGGCG	65	0.0	42.46154	32
TGGGATA	3300	0.0	42.166664	6
TGGGATT	11460	0.0	42.126526	6
TTGGGGC	29420	0.0	42.07546	5
TTGGGGG	49260	0.0	41.797806	5
>>END_MODULE