FastQCFastQC Report
Fri 17 Jun 2016
SRR1527184_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527184_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2486972
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC133550.5369984060938362No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG81550.32790879832985653No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT81160.3263406262716267No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG74530.2996817012817193No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT68910.2770839398272276No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC57310.23044087347987835No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG49230.19795156519655227No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT48790.19618234543854937No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT48610.19545857371936637No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA46280.1860897509099419No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC45180.18166670151493464No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG39210.15766160616203156No Hit
TCACTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC36580.14708649715396876No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT34660.13936626548268335No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT32820.13196771013103487No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT28180.11331048359209511No Hit
TCACTTGGGACATTTTGTTTTTGAGACTGTATTCCCTAGCATATATCTCCTA26570.10683674765940268No Hit
TCACTTGGGAGGACGTGTTTATATTGAGTCACATTCAAGACCTGGGCTGGCC25610.10297663182375998No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC25220.10140845976553013No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA206.312878E-446.026
ATAGCCG301.8622832E-646.023
CACGATA301.8622832E-646.045
GTAATCG650.046.014
TACGATA206.312878E-446.034
CGAATAC301.8622832E-646.034
CATACGC301.8622832E-646.018
TCGACTA501.6370905E-1146.028
TATCGTA301.8622832E-646.026
CGATAGT405.6152203E-946.019
TACCGTA600.046.040
CATTTCG1300.044.2307712
TTGGGAT280450.043.4330525
TCACTTG2646200.043.094361
CACTTGG2639050.043.029842
TTGGGAC173050.042.929795
TTGGGAG339650.042.9188885
ACTTGGG2587250.042.8636933
CTTGGGA967050.042.853424
CTTGGGG1314850.042.6449434
Produced by FastQC (version 0.11.5)