##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527184_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2486972
Sequences flagged as poor quality	0
Sequence length	52
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.58266598900189	34.0	31.0	34.0	31.0	34.0
2	32.81215470057564	34.0	31.0	34.0	31.0	34.0
3	32.921707200563574	34.0	31.0	34.0	31.0	34.0
4	36.45563399990028	37.0	37.0	37.0	35.0	37.0
5	36.327988011123566	37.0	37.0	37.0	35.0	37.0
6	36.48995404853774	37.0	37.0	37.0	35.0	37.0
7	36.550032328470124	37.0	37.0	37.0	35.0	37.0
8	36.66276620725927	37.0	37.0	37.0	35.0	37.0
9	38.66594517348808	39.0	39.0	39.0	39.0	39.0
10	38.146121468195055	39.0	39.0	39.0	37.0	39.0
11	37.8514366064435	39.0	38.0	39.0	35.0	39.0
12	37.49004934514743	39.0	37.0	39.0	35.0	39.0
13	37.369857400887504	39.0	37.0	39.0	35.0	39.0
14	38.25663376990171	40.0	38.0	40.0	35.0	40.0
15	38.321843993418504	40.0	38.0	40.0	35.0	40.0
16	38.327926892622834	40.0	38.0	40.0	35.0	40.0
17	38.28227539353077	40.0	38.0	40.0	35.0	40.0
18	38.27744622778222	40.0	38.0	40.0	34.0	40.0
19	38.22454173187314	40.0	38.0	40.0	34.0	40.0
20	38.214279855181324	40.0	38.0	40.0	34.0	40.0
21	33.948975300083795	40.0	35.0	40.0	10.0	40.0
22	34.78030995121779	40.0	35.0	40.0	18.0	40.0
23	36.85673139866472	40.0	35.0	40.0	31.0	40.0
24	37.61724096612266	40.0	37.0	40.0	34.0	40.0
25	37.88418446206873	40.0	37.0	40.0	34.0	40.0
26	37.95011443635071	40.0	38.0	40.0	34.0	40.0
27	37.9176122610146	40.0	38.0	40.0	34.0	40.0
28	37.834846552353625	40.0	37.0	40.0	34.0	40.0
29	37.80455429333342	40.0	37.0	40.0	34.0	40.0
30	37.70781858420602	40.0	37.0	40.0	34.0	40.0
31	37.57147326146012	40.0	37.0	40.0	33.0	40.0
32	37.50776003911584	40.0	36.0	40.0	33.0	40.0
33	37.16596326778106	40.0	36.0	40.0	33.0	40.0
34	37.07079170975789	40.0	36.0	40.0	32.0	40.0
35	37.01437048748438	39.0	35.0	40.0	32.0	40.0
36	36.963381171963334	39.0	35.0	40.0	32.0	40.0
37	36.87758567446678	39.0	35.0	40.0	32.0	40.0
38	36.81492513787851	39.0	35.0	40.0	32.0	40.0
39	36.67031474419495	39.0	35.0	40.0	31.0	40.0
40	31.759058807256373	38.0	31.0	40.0	9.0	40.0
41	32.70633163541849	37.0	31.0	40.0	15.0	40.0
42	34.55925157179092	37.0	32.0	40.0	25.0	40.0
43	35.459490898972724	37.0	34.0	40.0	29.0	40.0
44	36.115235314269725	38.0	35.0	40.0	30.0	40.0
45	36.33005236890484	39.0	35.0	40.0	31.0	40.0
46	36.3630390691974	39.0	35.0	40.0	31.0	40.0
47	36.279740182036626	38.0	35.0	40.0	31.0	40.0
48	36.1467901528445	38.0	35.0	40.0	31.0	40.0
49	36.01478625412751	38.0	35.0	40.0	31.0	40.0
50	35.87474607675519	37.0	35.0	40.0	31.0	40.0
51	35.70855763555038	37.0	35.0	40.0	30.0	40.0
52	35.32736878420827	37.0	34.0	40.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	25.0
15	19.0
16	37.0
17	116.0
18	254.0
19	492.0
20	889.0
21	1597.0
22	2526.0
23	4103.0
24	6131.0
25	9071.0
26	12679.0
27	17010.0
28	23439.0
29	31078.0
30	41007.0
31	53888.0
32	79610.0
33	91459.0
34	163485.0
35	243151.0
36	317217.0
37	312695.0
38	631278.0
39	443711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.69581483024336	0.22127309837022693	0.05854509017391431	0.024366981212494553
8	99.60055843009089	0.3054316654952287	0.05339826905972403	0.040611635354157585
9	97.55871799119572	1.3221298832475799	0.4522366958695152	0.6669154296871859
10	49.80277220652263	37.418274110042255	5.13628621472216	7.642667468712958
11	34.29700857106553	22.17970286758355	23.904651922096427	19.618636639254483
12	30.280316786839578	15.533387589405912	27.850816173242	26.335479450512512
13	23.906984075413796	17.317243619952297	29.98063508555786	28.79513721907605
14	24.565133825390877	18.4860947368929	30.821939290028194	26.126832147688035
15	26.716062746182907	18.63390500576605	28.820469229247454	25.82956301880359
16	30.903645075215962	19.52816517435661	25.757105427805378	23.811084322622047
17	31.56726332262687	20.64631206141444	23.047062853944475	24.73936176201421
18	29.311789597952853	19.83110384837465	24.838277230302552	26.018829323369946
19	29.225218458430575	20.14292883072266	25.100805316666214	25.531047394180558
20	26.90247417341249	21.869486266833725	25.974558619879918	25.253480939873867
21	33.59820697619435	19.622818431409765	25.09425116165361	21.684723430742284
22	28.86156337908107	19.229327873413933	25.933223212806578	25.975885534698424
23	28.439041533238008	20.99303892444306	25.003216763196367	25.564702779122563
24	28.645075215965438	21.140125421597027	24.921752235248327	25.29304712718921
25	27.30428810617892	20.245141481287284	24.494123777831035	27.956446634702765
26	27.303081819980278	19.828007713798147	25.10104657390594	27.767863892315635
27	26.78260953480779	18.597716419806897	24.52050123604126	30.099172809344054
28	28.565942841334763	22.059436133579307	22.45236375801577	26.922257267070155
29	30.144770427652585	21.0465980316626	23.823428651388117	24.985202889296705
30	29.333301701828567	20.527533080388523	23.47593780710036	26.66322741068255
31	31.285273818925184	20.959544377660865	23.23074003245714	24.52444177095681
32	32.510136825022556	20.372806770643177	23.321291916434923	23.79576448789934
33	31.58346776722858	20.707832657544998	24.72150872627436	22.987190848952057
34	28.310129748143524	22.66414740495671	25.646408564310335	23.37931428258943
35	27.194435643022917	25.73072796959515	25.3548893996394	21.719946987742524
36	28.29883086741628	25.327627331550172	22.98224507553764	23.391296725495906
37	28.87511399404577	26.033666643613195	21.935228864659514	23.15599049768152
38	30.751854061887308	25.13618971182627	21.77318441864243	22.338771807643994
39	29.55425312387916	23.45414423644496	21.76228763331473	25.229315006361148
40	28.459709236774682	28.03059302637907	20.704736522968492	22.804961213877757
41	24.315874887212242	24.185274301439662	23.931913990185656	27.56693682116244
42	24.485760193520473	22.419231097093174	23.842286925626826	29.252721783759526
43	25.559917843867964	21.556052902887526	25.058223413854275	27.825805839390235
44	25.979343555134516	20.621181098942813	25.418541101387547	27.98093424453512
45	26.939667997870504	21.91986882039685	25.07269884823794	26.067764333494708
46	26.896442742419296	22.50564139845563	24.326811882079895	26.27110397704518
47	26.02510201160286	22.62064068272582	24.90068243631211	26.453574869359205
48	27.119444851007568	21.65826555345215	25.380261619350758	25.84202797618952
49	27.055471472939786	21.839329111867762	25.40354294298448	25.70165647220797
50	25.09043125535792	23.16769147380831	24.921832654328234	26.820044616505534
51	24.334491904211227	23.31393357062323	25.37989973349117	26.97167479167437
52	26.13507510337873	21.914802418362573	25.751275044511964	26.198847433746742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	24.5
6	46.0
7	31.5
8	17.0
9	500.0
10	983.0
11	1949.0
12	2915.0
13	4313.5
14	5455.0
15	5198.0
16	5195.5
17	5193.0
18	5467.0
19	5741.0
20	5747.0
21	5753.0
22	7503.5
23	9254.0
24	9576.5
25	9899.0
26	12789.0
27	15679.0
28	16139.5
29	16600.0
30	20449.0
31	24298.0
32	27171.0
33	30044.0
34	31076.0
35	32108.0
36	39081.5
37	46055.0
38	49851.5
39	58247.5
40	62847.0
41	64878.5
42	66910.0
43	73238.0
44	79566.0
45	86182.0
46	92798.0
47	94771.0
48	96744.0
49	107309.5
50	117875.0
51	130750.0
52	143625.0
53	157296.5
54	170968.0
55	191506.0
56	212044.0
57	231115.5
58	250187.0
59	238179.5
60	226172.0
61	212743.0
62	199314.0
63	173098.0
64	129978.0
65	113074.0
66	93880.0
67	74686.0
68	66505.5
69	58325.0
70	49673.0
71	41021.0
72	38815.5
73	36610.0
74	26724.0
75	16838.0
76	12357.5
77	7877.0
78	5049.5
79	2222.0
80	1645.5
81	1069.0
82	602.5
83	136.0
84	76.0
85	16.0
86	13.5
87	11.0
88	7.0
89	2.5
90	2.0
91	3.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2486972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.285462979740803
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.27230775509601	5.739531414260597
2	10.158660956831742	1.6833841856313532
3	4.39398356860458	1.092185645737879
4	2.2912276043916933	0.7593552597739033
5	1.3345746053524539	0.5528784243174973
6	0.8095676020522361	0.40245854378408014
7	0.5684019263596948	0.3296631182926626
8	0.3731642089332699	0.24734705907846957
9	0.3063063612688417	0.22841010150768895
>10	4.12302595767866	9.317795527262723
>50	3.1832508176572425	19.18903824988355
>100	3.0038726409913825	43.81716384460652
>500	0.12300217828514474	6.7501792101264915
>1k	0.05523708932249556	7.545619607307691
>5k	0.002847272645489462	1.7148749437064976
>10k+	5.694545290978924E-4	0.6301148647223979
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCACTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13355	0.5369984060938362	No Hit
TCACTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	8155	0.32790879832985653	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8116	0.3263406262716267	No Hit
TCACTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCACTTGGGAAG	7453	0.2996817012817193	No Hit
TCACTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	6891	0.2770839398272276	No Hit
TCACTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5731	0.23044087347987835	No Hit
TCACTTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4923	0.19795156519655227	No Hit
TCACTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	4879	0.19618234543854937	No Hit
TCACTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4861	0.19545857371936637	No Hit
TCACTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4628	0.1860897509099419	No Hit
TCACTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4518	0.18166670151493464	No Hit
TCACTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3921	0.15766160616203156	No Hit
TCACTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3658	0.14708649715396876	No Hit
TCACTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3466	0.13936626548268335	No Hit
TCACTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	3282	0.13196771013103487	No Hit
TCACTTGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	2818	0.11331048359209511	No Hit
TCACTTGGGACATTTTGTTTTTGAGACTGTATTCCCTAGCATATATCTCCTA	2657	0.10683674765940268	No Hit
TCACTTGGGAGGACGTGTTTATATTGAGTCACATTCAAGACCTGGGCTGGCC	2561	0.10297663182375998	No Hit
TCACTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2522	0.10140845976553013	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.608381598184459E-4	0.0	0.0	0.0	0.0
9	3.618858595915032E-4	0.0	0.0	0.0	0.0
10	0.006071640533146332	0.0	0.0	0.0	0.0
11	0.013309357724976397	0.0	0.0	0.0	0.0
12	0.01825513113939361	0.0	0.0	0.0	0.0
13	0.02267818053440087	0.0	0.0	0.0	0.0
14	0.035665861939740376	0.0	0.0	0.0	0.0
15	0.042702531431797384	0.0	0.0	0.0	0.0
16	0.0581832043143228	0.0	0.0	0.0	0.0
17	0.06377233036801379	0.0	0.0	0.0	0.0
18	0.06920061826188634	0.0	0.0	0.0	0.0
19	0.07728273579276325	0.0	0.0	0.0	0.0
20	0.08508338654395788	0.0	0.0	0.0	0.0
21	0.09573891463192992	0.0	0.0	0.0	0.0
22	0.1037406130828976	0.0	0.0	0.0	0.0
23	0.11190314969368373	0.0	0.0	0.0	0.0
24	0.12593627913784314	0.0	0.0	0.0	0.0
25	0.13236980553058098	0.0	0.0	0.0	0.0
26	0.14000961812195714	0.0	0.0	0.0	0.0
27	0.1479308974930156	0.0	0.0	0.0	0.0
28	0.16815629609018518	0.0	0.0	0.0	0.0
29	0.17515275604228756	0.0	0.0	0.0	0.0
30	0.1856876555103958	0.0	0.0	0.0	0.0
31	0.19421207798077342	0.0	0.0	0.0	0.0
32	0.2035809007901979	0.0	0.0	0.0	0.0
33	0.21126092292152868	0.0	0.0	0.0	0.0
34	0.21857905919326795	0.0	0.0	0.0	0.0
35	0.24206143052676105	0.0	0.0	0.0	0.0
36	0.2538830352734168	0.0	0.0	0.0	0.0
37	0.2650210778408442	0.0	0.0	0.0	0.0
38	0.27531472006922475	0.0	0.0	0.0	0.0
39	0.28480417149851306	0.0	0.0	0.0	0.0
40	0.29533907096662126	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	6.312878E-4	46.0	26
ATAGCCG	30	1.8622832E-6	46.0	23
CACGATA	30	1.8622832E-6	46.0	45
GTAATCG	65	0.0	46.0	14
TACGATA	20	6.312878E-4	46.0	34
CGAATAC	30	1.8622832E-6	46.0	34
CATACGC	30	1.8622832E-6	46.0	18
TCGACTA	50	1.6370905E-11	46.0	28
TATCGTA	30	1.8622832E-6	46.0	26
CGATAGT	40	5.6152203E-9	46.0	19
TACCGTA	60	0.0	46.0	40
CATTTCG	130	0.0	44.23077	12
TTGGGAT	28045	0.0	43.433052	5
TCACTTG	264620	0.0	43.09436	1
CACTTGG	263905	0.0	43.02984	2
TTGGGAC	17305	0.0	42.92979	5
TTGGGAG	33965	0.0	42.918888	5
ACTTGGG	258725	0.0	42.863693	3
CTTGGGA	96705	0.0	42.85342	4
CTTGGGG	131485	0.0	42.644943	4
>>END_MODULE