FastQCFastQC Report
Fri 17 Jun 2016
SRR1527183_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527183_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084755
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC91820.8464584168775438No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG40920.37722803766749174No Hit
ACGAGCGGGATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGTTAAATGGG36190.33362372148549674No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC35210.3245894234181912No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC33520.3090098685878378No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA33270.3067052007135252No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG31140.28706943042438154No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC30300.27932574636669105No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT28560.26328525796147517No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC28280.260704029942245No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC26470.24401823453222157No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT25810.2379339113440362No Hit
ACGAGCGGGGTTCAAATGCCTCTGTTTATTCAGTACCACCTCCTGCAGCT25780.23765735119911868No Hit
ACGAGCGGGGGGCATTAAAAACGTTTCCACTACAAATTTATTGTTACTGA24400.22493558453291299No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT22830.21046227028222964No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC22150.20419357366409924No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG21930.20216546593470416No Hit
ACGAGCGGCCGTTTGTGATCATCATCTTCTGTTACACGACTCTCATCCAC21430.19755613018607887No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT19770.18225313550064298No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT19140.17644537245737518No Hit
ACGAGCGGGGACTATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAG18460.17017667583924478No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT18120.1670423275301796No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG18070.1665813939553171No Hit
ACGAGCGGGAACTTAGCTTATGTATCTATGTATGTATGTATGTATGCATG17990.16584390023553705No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA17530.1616033113468018No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCTGACTGC16540.1524768265645238No Hit
ACGAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA16470.15183151955971624No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC16440.1515549594147987No Hit
ACGAGCGGGGCAGATGTCTGTTCTCTGGGCTTTATGTTCTGTACAGTAGA15650.14427220893197082No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15600.14381127535710828No Hit
ACGAGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC15360.14159879419776816No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG14910.13745039202400544No Hit
ACGAGCGGGCATGGAGACTGTTTCTCAAAAAAACAAATTACCCCCTCAAA14270.13155044226576507No Hit
ACGAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT14030.12933796110642495No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC13790.12712547994708484No Hit
ACGAGCGGCAGTAGCTGGGGTCAGAATGGAATAAAGGGAGAAAGAACGCT13380.12334582463321211No Hit
ACGAGCGGGGGGTTTTTGGGTCTCTGTACACTTAATCAATTTCTAACAAA12450.11477246014076911No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA12440.1146802734257966No Hit
ACGAGCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC12390.11421933985093409No Hit
ACGAGCGGGATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCC12110.11163811183170394No Hit
ACGAGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG11910.10979437753225382No Hit
ACGAGCGGGGCCGGGCAGTGGTGGCACACGCCTTTGATCCCAGCACTTGG11900.10970219081728133No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGTTTTCTGACAATACCAAAGAA11900.10970219081728133No Hit
ACGAGCGGGGTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC11590.10684440265313366No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC11500.10601472221838111No Hit
ACGAGCGGGAGATCCCATAGAAGCATGGGGCCAAGGATTGGTGGCACCAA11390.10500066835368355No Hit
ACGAGCGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC10890.1003913326050583No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTTG207.856285E-444.00000443
ATTAGTA207.856285E-444.00000440
CGTATGA207.856285E-444.00000416
CGTATCT207.856285E-444.00000433
GCGAATA207.856285E-444.00000417
TATGGTA207.856285E-444.00000423
CACGCTA408.310963E-944.00000413
ATTGTCG207.856285E-444.00000421
CGATTGG207.856285E-444.00000423
TAACGCG207.856285E-444.00000423
TGCGATA207.856285E-444.00000414
TAATTAG650.044.00000433
TAATTAC207.856285E-444.00000423
TGCGAAA207.856285E-444.00000430
GTCGTTA207.856285E-444.00000425
CAAATAG408.310963E-944.00000426
ATAAGAT207.856285E-444.00000426
CGAATAG207.856285E-444.00000418
GCGATTT207.856285E-444.00000440
TAGATTG207.856285E-444.00000429
Produced by FastQC (version 0.11.5)