##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527183_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1084755
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43079405026941	33.0	31.0	34.0	31.0	34.0
2	32.77115385501796	34.0	31.0	34.0	31.0	34.0
3	32.91221520066743	34.0	31.0	34.0	31.0	34.0
4	36.28080810874345	37.0	37.0	37.0	35.0	37.0
5	36.25010624518901	37.0	37.0	37.0	35.0	37.0
6	36.08124415190527	37.0	37.0	37.0	35.0	37.0
7	36.53077607386	37.0	37.0	37.0	35.0	37.0
8	36.66354799009915	37.0	37.0	37.0	35.0	37.0
9	38.75842102594595	39.0	39.0	39.0	39.0	39.0
10	38.23195007167517	39.0	39.0	39.0	37.0	39.0
11	37.706444312310154	39.0	38.0	39.0	35.0	39.0
12	36.967087499020515	39.0	37.0	39.0	33.0	39.0
13	36.85407857073717	39.0	37.0	39.0	33.0	39.0
14	37.62121031938087	40.0	37.0	40.0	33.0	40.0
15	37.63930011845993	40.0	37.0	40.0	33.0	40.0
16	37.50031666136593	40.0	37.0	40.0	33.0	40.0
17	37.46143414872483	40.0	36.0	40.0	33.0	40.0
18	37.41144682439813	39.0	36.0	40.0	33.0	40.0
19	37.323166982406164	39.0	36.0	40.0	33.0	40.0
20	36.993813349558195	39.0	35.0	40.0	32.0	40.0
21	37.06210158054123	39.0	35.0	40.0	32.0	40.0
22	36.836740093385146	39.0	35.0	40.0	32.0	40.0
23	36.798918649833375	39.0	35.0	40.0	32.0	40.0
24	36.78954326092067	38.0	35.0	40.0	32.0	40.0
25	36.640219220008206	38.0	35.0	40.0	32.0	40.0
26	36.42324488018032	38.0	35.0	40.0	31.0	40.0
27	36.20543532871478	38.0	35.0	40.0	31.0	40.0
28	35.89056330692184	37.0	34.0	40.0	30.0	40.0
29	36.21398841212993	38.0	35.0	40.0	31.0	40.0
30	36.14561168190052	38.0	35.0	40.0	31.0	40.0
31	36.07772999433052	37.0	35.0	40.0	31.0	40.0
32	35.3091315550516	37.0	34.0	40.0	30.0	40.0
33	35.42588234209568	36.0	34.0	40.0	30.0	40.0
34	35.272811833086735	36.0	34.0	40.0	30.0	40.0
35	35.28815815552821	36.0	34.0	40.0	30.0	40.0
36	35.11809118187978	35.0	34.0	40.0	30.0	40.0
37	34.999722517987934	35.0	34.0	39.0	29.0	40.0
38	34.76667911187319	35.0	34.0	39.0	29.0	40.0
39	34.71219399772299	35.0	34.0	39.0	29.0	40.0
40	34.57794985964573	35.0	34.0	39.0	29.0	40.0
41	34.41562288258639	35.0	34.0	39.0	28.0	40.0
42	34.27050808707957	35.0	33.0	38.0	28.0	40.0
43	34.17282612202755	35.0	33.0	38.0	28.0	40.0
44	34.05888426418869	35.0	33.0	37.0	28.0	40.0
45	33.93072537116676	35.0	33.0	37.0	27.0	40.0
46	33.67566685564943	35.0	33.0	37.0	26.0	40.0
47	33.557965623573985	35.0	33.0	37.0	26.0	40.0
48	33.42839443007868	35.0	33.0	36.0	26.0	40.0
49	33.201979248770456	35.0	33.0	36.0	25.0	40.0
50	32.831364686035094	35.0	32.0	36.0	24.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	2.0
13	0.0
14	5.0
15	16.0
16	58.0
17	122.0
18	257.0
19	462.0
20	870.0
21	1423.0
22	2162.0
23	3127.0
24	4277.0
25	5975.0
26	7889.0
27	10372.0
28	13622.0
29	17787.0
30	23228.0
31	29626.0
32	38558.0
33	56326.0
34	106139.0
35	141280.0
36	190566.0
37	174917.0
38	199058.0
39	56630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.10016547515337	0.7212688579448815	0.16095800434199428	0.017607662559748515
8	99.64249991933663	0.2341542560301635	0.029776308936119215	0.09356951569709289
9	98.59055731478537	0.47651312969287996	0.25959778936257494	0.6733317661591788
10	62.82432438661264	27.85274094150292	2.9241625989278686	6.398772072956565
11	36.62255532355233	23.201828984425056	22.351037791943803	17.82457790007882
12	30.869735562408103	15.134846117326031	26.915386423662486	27.08003189660338
13	23.025660172112598	16.134703227917825	31.107946033897054	29.731690566072523
14	25.48741420873838	18.096620895962683	30.87517457859148	25.540790316707458
15	26.191536337698373	17.84329180321824	29.75722628611991	26.20794557296348
16	30.89250568100631	17.58839553631926	25.95212743891478	25.566971343759654
17	31.305317790653188	18.283206807067035	24.505349134136278	25.9061262681435
18	29.33233771681163	18.55165452106697	24.362551912643866	27.753455849477533
19	30.018759996496904	20.301911491534955	23.03248198902056	26.646846522947577
20	29.169259418025266	21.311263833768916	24.071795013620587	25.44768173458523
21	29.28006784942222	18.088600651760075	26.200017515475842	26.43131398334186
22	29.225769874303413	18.23231974040221	26.237629695184626	26.30428069010975
23	28.658821577222504	19.76483169010514	25.045010163585324	26.531336569087028
24	30.409355107835413	19.949205120050152	22.608515286862012	27.03292448525243
25	27.88104226299948	20.53219390553627	22.481389806914926	29.105374024549324
26	27.164013993943332	20.289374098298694	24.45722766892063	28.08938423883734
27	28.835635696539775	17.631170172066504	23.372927527414024	30.1602666039797
28	29.310858212223035	22.105636756686994	21.327765255748993	27.255739775340977
29	30.373771035856024	20.02572009347733	22.750667201349614	26.849841669317037
30	28.24730008158524	18.335845421316336	24.620766901281858	28.796087595816566
31	30.998796963369607	20.875958165668745	22.644468105701286	25.48077676526036
32	31.8786269710672	20.80110255311107	22.20160312697337	25.118667348848355
33	32.70305276306632	17.853155781720297	23.597309991657102	25.846481463556287
34	27.871362657927367	22.56251411609073	26.70750538139949	22.85861784458242
35	27.97415084512171	24.717194205143098	25.26699577323912	22.041659176496076
36	30.708961931496052	24.4851602435573	21.531958829413092	23.273918995533556
37	30.876926126175956	22.632668206184807	20.924171817599365	25.566233850039872
38	29.818530451576624	24.93954856165678	22.04036856248646	23.201552424280138
39	31.88941281671898	20.827652327023152	22.278671220690384	25.00426363556748
40	28.134555729173872	22.59819037478509	22.363022064890227	26.904231831150817
41	27.111098819549113	21.91868209872275	23.767302294066404	27.202916787661728
42	25.458098833377125	19.86817299758932	24.050960816036802	30.62276735299676
43	26.385865932860415	18.71943434231693	26.059893708717635	28.83480601610502
44	26.395268977787612	19.167738337228222	25.0109010790455	29.42609160593867
45	27.9909288272467	20.535604813990254	24.88174749136902	26.59171886739402
46	27.448133449488594	22.205935902577078	23.77818032643316	26.567750321501173
47	26.755995593475024	19.995759411111262	26.14608828721693	27.102156708196784
48	28.848726210065866	20.647150739106987	25.475429935791954	25.028693115035196
49	29.217104323096	21.22387082797498	22.858433471152473	26.70059137777655
50	25.93046356089624	21.022258482330113	24.24372323704431	28.80355471972934
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	98.0
14	194.0
15	696.5
16	1199.0
17	1182.5
18	1166.0
19	1352.0
20	1538.0
21	1582.5
22	1627.0
23	2113.0
24	2599.0
25	2604.5
26	2610.0
27	2629.5
28	2649.0
29	3152.0
30	3655.0
31	3547.5
32	3440.0
33	5585.0
34	7730.0
35	7798.0
36	7866.0
37	10850.5
38	13835.0
39	14711.5
40	15588.0
41	15786.0
42	15984.0
43	18877.5
44	21771.0
45	22157.0
46	22543.0
47	27725.5
48	32908.0
49	37019.5
50	41131.0
51	42209.5
52	43288.0
53	45112.5
54	46937.0
55	52043.0
56	57149.0
57	64714.5
58	72280.0
59	83567.5
60	94855.0
61	102065.5
62	109276.0
63	110794.0
64	112312.0
65	113113.0
66	113914.0
67	96783.5
68	79653.0
69	66196.5
70	52740.0
71	43484.5
72	34229.0
73	29522.0
74	24815.0
75	21433.0
76	18051.0
77	16602.0
78	15153.0
79	11029.5
80	6906.0
81	4275.5
82	1645.0
83	1227.0
84	809.0
85	639.0
86	469.0
87	326.5
88	184.0
89	106.5
90	29.0
91	20.5
92	12.0
93	9.5
94	7.0
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1084755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.76276534377184
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.29028200149644	6.764647637843872
2	11.51744526596221	2.248842309826498
3	4.596765296724511	1.3463142279694542
4	2.276296522817481	0.888917952204434
5	1.2820611486732267	0.6258231075431647
6	0.7854402065868323	0.46008410570825525
7	0.47374241551672763	0.3237525225257027
8	0.3507790904373287	0.2739659157953088
9	0.24497344078573785	0.215245639606279
>10	3.846178340640019	10.173124978598798
>50	2.7881218454450574	19.66515059003861
>100	2.4001677972070614	40.71018113857169
>500	0.09722681307193021	6.3632864635752115
>1k	0.0495666105856899	9.08619498701385
>5k	9.53204049724806E-4	0.8544684231788647
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	9182	0.8464584168775438	No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	4092	0.37722803766749174	No Hit
ACGAGCGGGATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGTTAAATGGG	3619	0.33362372148549674	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3521	0.3245894234181912	No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3352	0.3090098685878378	No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3327	0.3067052007135252	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3114	0.28706943042438154	No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	3030	0.27932574636669105	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2856	0.26328525796147517	No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2828	0.260704029942245	No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2647	0.24401823453222157	No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2581	0.2379339113440362	No Hit
ACGAGCGGGGTTCAAATGCCTCTGTTTATTCAGTACCACCTCCTGCAGCT	2578	0.23765735119911868	No Hit
ACGAGCGGGGGGCATTAAAAACGTTTCCACTACAAATTTATTGTTACTGA	2440	0.22493558453291299	No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2283	0.21046227028222964	No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2215	0.20419357366409924	No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2193	0.20216546593470416	No Hit
ACGAGCGGCCGTTTGTGATCATCATCTTCTGTTACACGACTCTCATCCAC	2143	0.19755613018607887	No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	1977	0.18225313550064298	No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	1914	0.17644537245737518	No Hit
ACGAGCGGGGACTATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAG	1846	0.17017667583924478	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1812	0.1670423275301796	No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1807	0.1665813939553171	No Hit
ACGAGCGGGAACTTAGCTTATGTATCTATGTATGTATGTATGTATGCATG	1799	0.16584390023553705	No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1753	0.1616033113468018	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCTGACTGC	1654	0.1524768265645238	No Hit
ACGAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1647	0.15183151955971624	No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1644	0.1515549594147987	No Hit
ACGAGCGGGGCAGATGTCTGTTCTCTGGGCTTTATGTTCTGTACAGTAGA	1565	0.14427220893197082	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1560	0.14381127535710828	No Hit
ACGAGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCC	1536	0.14159879419776816	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1491	0.13745039202400544	No Hit
ACGAGCGGGCATGGAGACTGTTTCTCAAAAAAACAAATTACCCCCTCAAA	1427	0.13155044226576507	No Hit
ACGAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1403	0.12933796110642495	No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1379	0.12712547994708484	No Hit
ACGAGCGGCAGTAGCTGGGGTCAGAATGGAATAAAGGGAGAAAGAACGCT	1338	0.12334582463321211	No Hit
ACGAGCGGGGGGTTTTTGGGTCTCTGTACACTTAATCAATTTCTAACAAA	1245	0.11477246014076911	No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGA	1244	0.1146802734257966	No Hit
ACGAGCGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC	1239	0.11421933985093409	No Hit
ACGAGCGGGATCTATGATATCTGTCGTAGAAACCTCGACATTGAGCGCCC	1211	0.11163811183170394	No Hit
ACGAGCGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	1191	0.10979437753225382	No Hit
ACGAGCGGGGCCGGGCAGTGGTGGCACACGCCTTTGATCCCAGCACTTGG	1190	0.10970219081728133	No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGTTTTCTGACAATACCAAAGAA	1190	0.10970219081728133	No Hit
ACGAGCGGGGTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAAC	1159	0.10684440265313366	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC	1150	0.10601472221838111	No Hit
ACGAGCGGGAGATCCCATAGAAGCATGGGGCCAAGGATTGGTGGCACCAA	1139	0.10500066835368355	No Hit
ACGAGCGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC	1089	0.1003913326050583	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.374937197800425E-4	0.0	0.0	0.0	0.0
8	0.0011062405796700638	0.0	0.0	0.0	0.0
9	0.001198427294642569	0.0	0.0	0.0	0.0
10	0.0027656014491751592	0.0	0.0	0.0	0.0
11	0.005715576328295329	0.0	0.0	0.0	0.0
12	0.008204617632552973	0.0	0.0	0.0	0.0
13	0.011246779226645649	0.0	0.0	0.0	0.0
14	0.015763928260298408	0.0	0.0	0.0	0.0
15	0.021387317873621234	0.0	0.0	0.0	0.0
16	0.028762255071421657	9.218671497250531E-5	0.0	0.0	0.0
17	0.03244972367032187	9.218671497250531E-5	0.0	0.0	0.0
18	0.03715124613391964	9.218671497250531E-5	0.0	0.0	0.0
19	0.04323556932210499	9.218671497250531E-5	0.0	0.0	0.0
20	0.04563242391139013	9.218671497250531E-5	0.0	0.0	0.0
21	0.049688639370180365	9.218671497250531E-5	0.0	0.0	0.0
22	0.05448234854875064	9.218671497250531E-5	0.0	0.0	0.0
23	0.058907310867430895	9.218671497250531E-5	0.0	0.0	0.0
24	0.06231821932141359	9.218671497250531E-5	0.0	0.0	0.0
25	0.06692755507003886	9.218671497250531E-5	0.0	0.0	0.0
26	0.0711681439587741	9.218671497250531E-5	0.0	0.0	0.0
27	0.07540873284750935	9.218671497250531E-5	0.0	0.0	0.0
28	0.07964932173624459	9.218671497250531E-5	0.0	0.0	0.0
29	0.0831524169051998	9.218671497250531E-5	0.0	0.0	0.0
30	0.08757737922388005	9.218671497250531E-5	0.0	0.0	0.0
31	0.09430700941687294	9.218671497250531E-5	0.0	0.0	0.0
32	0.09946946545533324	9.218671497250531E-5	0.0	0.0	0.0
33	0.1040788012039585	9.218671497250531E-5	0.0	0.0	0.0
34	0.10961000410230881	9.218671497250531E-5	0.0	0.0	0.0
35	0.11882867559955934	9.218671497250531E-5	0.0	0.0	0.0
36	0.12896921424653493	9.218671497250531E-5	0.0	0.0	0.0
37	0.13486916400477528	9.218671497250531E-5	0.0	0.0	0.0
38	0.13855663260367548	9.218671497250531E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTG	20	7.856285E-4	44.000004	43
ATTAGTA	20	7.856285E-4	44.000004	40
CGTATGA	20	7.856285E-4	44.000004	16
CGTATCT	20	7.856285E-4	44.000004	33
GCGAATA	20	7.856285E-4	44.000004	17
TATGGTA	20	7.856285E-4	44.000004	23
CACGCTA	40	8.310963E-9	44.000004	13
ATTGTCG	20	7.856285E-4	44.000004	21
CGATTGG	20	7.856285E-4	44.000004	23
TAACGCG	20	7.856285E-4	44.000004	23
TGCGATA	20	7.856285E-4	44.000004	14
TAATTAG	65	0.0	44.000004	33
TAATTAC	20	7.856285E-4	44.000004	23
TGCGAAA	20	7.856285E-4	44.000004	30
GTCGTTA	20	7.856285E-4	44.000004	25
CAAATAG	40	8.310963E-9	44.000004	26
ATAAGAT	20	7.856285E-4	44.000004	26
CGAATAG	20	7.856285E-4	44.000004	18
GCGATTT	20	7.856285E-4	44.000004	40
TAGATTG	20	7.856285E-4	44.000004	29
>>END_MODULE