FastQCFastQC Report
Fri 17 Jun 2016
SRR1527182_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527182_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170530
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC82640.7060049721066526No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG39140.33437844395273936No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA38560.3294234235773538No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34590.29550716342169786No Hit
ACGAGCGGGATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGTTAAATGGGAA33760.2884163584017496No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT30550.2609928835655643No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA29090.24851990124131804No Hit
ACGAGCGGGGTTCAAATGCCTCTGTTTATTCAGTACCACCTCCTGCAGCTCT28840.2463841165967553No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27230.2326296634857714No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT27060.23117732992746876No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT25100.21443277831409702No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG24150.2063167966647587No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT24130.20614593389319366No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC21820.18641128377743416No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG21050.17983306707218097No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT21010.17949134152905094No Hit
ACGAGCGGCCGTTTGTGATCATCATCTTCTGTTACACGACTCTCATCCACAA19480.1664203395043271No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC18000.1537764944085158No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA17890.1528367491649082No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC17590.15027380759143294No Hit
ACGAGCGGGGACTATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTT17250.14736914047482763No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA16860.1440373164293098No Hit
ACGAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC16240.13874057051079425No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT15920.13600676616575397No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCTGACTGCTC15840.13532331507949388No Hit
ACGAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15740.1344690012216688No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC15710.13421270706432128No Hit
ACGAGCGGGAACTTAGCTTATGTATCTATGTATGTATGTATGTATGCATGTA15350.13113717717615098No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15300.13071002024723843No Hit
ACGAGCGGGGCAGATGTCTGTTCTCTGGGCTTTATGTTCTGTACAGTAGATT15010.1282325100595457No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA14930.1275490589732856No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13330.11388003724808421No Hit
ACGAGCGGGCATGGAGACTGTTTCTCAAAAAAACAAATTACCCCCTCAAAAC13130.11217140953243403No Hit
ACGAGCGGGGGGTTTTTGGGTCTCTGTACACTTAATCAATTTCTAACAAACT13040.11140252706039146No Hit
ACGAGCGGCAGTAGCTGGGGTCAGAATGGAATAAAGGGAGAAAGAACGCTGC12860.10986476211630629No Hit
ACGAGCGGGGGGCATTAAAAACGTTTCCACTACAAATTTATTGTTACTGATG12780.10918131103004622No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGTTTTCTGACAATACCAAAGAAAT12500.10678923222813598No Hit
ACGAGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT11760.10046730968023031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG351.0195981E-746.0000049
CATAGCG700.046.00000419
CAATACT351.0195981E-746.00000423
ATCGATT700.046.00000416
TATCACG206.311502E-446.020
TTAGGCG405.6115823E-946.036
AGCGTAC206.311502E-446.029
ATTAGGT206.311502E-446.043
TAACGCG206.311502E-446.020
ATGTCGT301.8614337E-646.011
CGTTACA253.417195E-546.018
AACGGAT253.417195E-546.037
CCGTATG253.417195E-546.012
ACGATAC206.311502E-446.022
CCGTAAC206.311502E-446.033
AATTGCG206.311502E-446.043
TACGACA405.6115823E-946.015
CGCTAGT253.417195E-546.014
ACTACCG206.311502E-446.025
CGAATTC206.311502E-446.017
Produced by FastQC (version 0.11.5)