FastQCFastQC Report
Fri 17 Jun 2016
SRR1527181_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527181_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135298
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC79140.6970856990851741No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG39430.34730969313783694No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA37250.3281076862638708No Hit
ACGAGCGGGATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGTTAAATGGGAA33610.29604561974036775No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC32990.2905844985193315No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32580.2869731119054204No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT26340.23200956929370084No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA25860.22778160447741472No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG25610.2255795394689324No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT25360.22337747446045003No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT24610.216771279435003No Hit
ACGAGCGGGGTTCAAATGCCTCTGTTTATTCAGTACCACCTCCTGCAGCTCT24610.216771279435003No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG23680.2085795976034486No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG21440.18884909512744671No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT20940.184444965110482No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC20060.17669369628062412No Hit
ACGAGCGGCCGTTTGTGATCATCATCTTCTGTTACACGACTCTCATCCACAA19540.17211340106298081No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA18110.15951758921446177No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC17670.1556419547995328No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC17480.15396838539308622No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC17220.1516782377842646No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA16620.14639328176390692No Hit
ACGAGCGGGGACTATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTT16340.14392696895440668No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT16100.14181298654626362No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15760.13881817813472763No Hit
ACGAGCGGGAACTTAGCTTATGTATCTATGTATGTATGTATGTATGCATGTA15140.13335705691369137No Hit
ACGAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15130.13326897431335208No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA14460.12736744009061937No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCTGACTGCTC14190.12498920988145844No Hit
ACGAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC13820.12173015366890455No Hit
ACGAGCGGGGCAGATGTCTGTTCTCTGGGCTTTATGTTCTGTACAGTAGATT13640.12014466686279726No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13460.11855918005668996No Hit
ACGAGCGGGGGGCATTAAAAACGTTTCCACTACAAATTTATTGTTACTGATG13050.11494779344277889No Hit
ACGAGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT12380.1090462592200462No Hit
ACGAGCGGGGGGTTTTTGGGTCTCTGTACACTTAATCAATTTCTAACAAACT12280.10816543321665324No Hit
ACGAGCGGGCATGGAGACTGTTTCTCAAAAAAACAAATTACCCCCTCAAAAC12090.10649186381020666No Hit
ACGAGCGGCAGTAGCTGGGGTCAGAATGGAATAAAGGGAGAAAGAACGCTGC11650.10261622939527772No Hit
ACGAGCGGGGCCGGGCAGTGGTGGCACACGCCTTTGATCCCAGCACTTGGGA11470.10103074258917043No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA11460.10094265998883112No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGTTTTCTGACAATACCAAAGAAAT11460.10094265998883112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC700.046.00000410
CGCTAGT351.01956175E-746.00000414
AACCGTT206.311423E-446.031
AGCGTAC206.311423E-446.029
CGAACCC301.8613828E-646.039
GACGTAA206.311423E-446.09
CGTTGAT301.8613828E-646.017
ATAGCCC206.311423E-446.010
TAACGCG206.311423E-446.020
TAACGAC206.311423E-446.011
TAACGAA206.311423E-446.039
AAACGAC206.311423E-446.019
TCAATCG405.6115823E-946.030
TAACCGT206.311423E-446.030
TACGCAA206.311423E-446.020
GAGGTAT206.311423E-446.09
CTAAAAT301.8613828E-646.035
CGAATTG253.4171302E-546.013
ACGCTTA301.8613828E-646.016
AATTCGC253.4171302E-546.017
Produced by FastQC (version 0.11.5)