##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527181_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1135298
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32537360234934	33.0	31.0	34.0	31.0	34.0
2	32.67588069387949	34.0	31.0	34.0	31.0	34.0
3	32.79414567805105	34.0	31.0	34.0	31.0	34.0
4	36.20992197643262	37.0	37.0	37.0	35.0	37.0
5	36.24347087724985	37.0	37.0	37.0	35.0	37.0
6	36.0673673343915	37.0	37.0	37.0	35.0	37.0
7	36.552616141312676	37.0	37.0	37.0	35.0	37.0
8	36.66342493336551	37.0	37.0	37.0	35.0	37.0
9	38.674907381145744	39.0	39.0	39.0	39.0	39.0
10	38.149476172775785	39.0	39.0	39.0	37.0	39.0
11	37.620391298143744	39.0	38.0	39.0	35.0	39.0
12	36.99017174345414	39.0	37.0	39.0	33.0	39.0
13	36.89370015625853	39.0	37.0	39.0	33.0	39.0
14	37.70413318793832	40.0	37.0	40.0	33.0	40.0
15	37.820964187376354	40.0	37.0	40.0	33.0	40.0
16	37.889032659266555	40.0	37.0	40.0	33.0	40.0
17	37.78836393616478	40.0	37.0	40.0	33.0	40.0
18	37.75727077824501	40.0	37.0	40.0	33.0	40.0
19	37.67211516271499	40.0	37.0	40.0	33.0	40.0
20	37.55152391706847	40.0	36.0	40.0	33.0	40.0
21	33.07608310769507	39.0	33.0	40.0	10.0	40.0
22	33.593913668481754	38.0	33.0	40.0	15.0	40.0
23	35.61456903826132	38.0	33.0	40.0	28.0	40.0
24	36.430981997678145	38.0	35.0	40.0	31.0	40.0
25	36.71841578158334	38.0	35.0	40.0	32.0	40.0
26	36.7393812021161	38.0	35.0	40.0	32.0	40.0
27	36.63796465773744	38.0	35.0	40.0	32.0	40.0
28	36.4149016381602	38.0	35.0	40.0	32.0	40.0
29	36.337928015375695	38.0	35.0	40.0	32.0	40.0
30	36.059409071450844	37.0	35.0	40.0	31.0	40.0
31	35.84404887527327	37.0	35.0	40.0	31.0	40.0
32	35.72101421829335	37.0	35.0	40.0	31.0	40.0
33	35.35245635947566	36.0	34.0	40.0	30.0	40.0
34	35.08159003186829	36.0	34.0	39.0	29.0	40.0
35	35.04885413345219	35.0	34.0	39.0	30.0	40.0
36	34.936771666998446	35.0	34.0	39.0	29.0	40.0
37	34.79562370408474	35.0	34.0	39.0	29.0	40.0
38	34.678287991346764	35.0	34.0	39.0	29.0	40.0
39	34.512586122762485	35.0	33.0	39.0	29.0	40.0
40	29.801815910888596	35.0	28.0	37.0	8.0	40.0
41	30.47377428657498	35.0	28.0	37.0	11.0	40.0
42	32.13568596086666	35.0	29.0	37.0	22.0	40.0
43	33.02398929620241	35.0	31.0	37.0	25.0	40.0
44	33.8595795993651	35.0	33.0	37.0	28.0	40.0
45	34.15774977142565	35.0	33.0	37.0	29.0	40.0
46	34.24101161104838	35.0	34.0	37.0	29.0	40.0
47	34.172292208741666	35.0	34.0	37.0	29.0	40.0
48	34.03111958269987	35.0	34.0	36.0	29.0	40.0
49	33.959570086444266	35.0	34.0	36.0	29.0	40.0
50	33.860613689093086	35.0	34.0	36.0	29.0	39.0
51	33.664715343460486	35.0	33.0	36.0	29.0	39.0
52	33.29510225509073	35.0	33.0	35.0	27.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	4.0
15	17.0
16	49.0
17	112.0
18	215.0
19	510.0
20	892.0
21	1350.0
22	2245.0
23	3373.0
24	4742.0
25	6647.0
26	8857.0
27	11757.0
28	15364.0
29	20252.0
30	27388.0
31	38639.0
32	58536.0
33	80044.0
34	124799.0
35	162680.0
36	199263.0
37	156919.0
38	162891.0
39	47751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.13309104746067	0.6984950206906028	0.15511345919749703	0.01330047265123342
8	99.66317213630253	0.23711836011337992	0.02131598928210919	0.07839351430197182
9	98.5610826408573	0.47238698561963466	0.297631106546475	0.6688992669766
10	63.358078671855324	27.37492711164822	2.8593373722141675	6.407656844282295
11	36.842309243916574	23.163786072026905	22.136831034671072	17.85707364938545
12	30.885282982970107	15.131621829687008	26.98789216575736	26.995203021585525
13	23.28534006049513	15.991748422000216	30.95803921085037	29.76487230665429
14	25.76424868184389	17.883234181686216	30.799050117237943	25.553467019231956
15	26.217433660589556	17.65959245942475	29.840711425546417	26.282262454439277
16	30.635656893608548	17.548079887395204	25.939533056519082	25.876730162477163
17	31.60544632334418	17.956078492166814	24.549149210163325	25.889325974325683
18	29.548893770622335	18.41586966593793	24.353429672209412	27.68180689123032
19	30.084612145885924	20.039231990191123	22.95802511763431	26.91813074628864
20	29.303231398276047	21.24525895403674	23.997575966838664	25.45393368084855
21	33.97795116348307	17.607535642624228	24.679863789066836	23.73464940482587
22	29.15349097769925	18.07023354220654	26.334847766841836	26.44142771325238
23	28.881315742650827	19.728124245792735	24.90632415453916	26.484235857017275
24	30.72426798954988	19.800704308472312	22.31678378716425	27.158243914813557
25	28.041976644017698	20.54271213373053	22.169597761997288	29.245713460254485
26	27.32630551626093	20.114542613481216	24.29635214718955	28.262799723068305
27	28.79059066430136	17.71702231484597	23.181402592094763	30.310984428757916
28	29.441168750407382	22.2653435485661	21.073145552973756	27.220342148052758
29	30.536211637825488	19.856372511886747	22.65017642944848	26.957239420839286
30	28.412628226245445	18.28964729965172	24.413061592639114	28.884662881463726
31	31.27090860725554	20.83356088005088	22.38777836303772	25.50775214965586
32	31.930206870795157	20.381873305510975	22.26569587896746	25.422223944726408
33	32.92219311581629	17.867026983223788	23.569142198788338	25.64163770217159
34	28.175245618331047	22.350167092692843	26.595484181245805	22.879103107730305
35	28.037308266199712	24.597418475149254	25.206245408694457	22.159027849956576
36	30.757563212478132	24.319605953679122	21.584993543545394	23.33783729029735
37	30.984287825751476	22.71262699308904	20.702405888145666	25.60067929301382
38	29.694230061182175	24.981810943029934	21.87645886806812	23.44750012771977
39	32.076071656957026	20.746182940514295	22.01545321140353	25.16229219112515
40	28.53700085792453	27.03387128313447	20.5437691249346	23.885358734006402
41	27.20853907960729	21.866857864631136	23.558308038946603	27.366295016814966
42	25.50907338866095	19.866502010925764	23.72214167557769	30.902282924835596
43	26.332381454032333	18.763619772077465	25.96965730583512	28.934341468055084
44	26.402319038701734	19.147395661755766	24.890909699479785	29.559375600062715
45	28.068489506719825	20.515142279824328	24.74451641771588	26.671851795739975
46	27.40073531354763	21.833650724303222	23.785473065221645	26.9801408969275
47	26.605613680284822	20.063718953085445	26.211708291567497	27.118959075062232
48	28.947377692905295	20.833737045251553	25.231348949791155	24.98753631205199
49	29.18062041860375	21.078694756795134	22.829688768939963	26.91099605566116
50	25.85902555980897	21.09155481644467	24.113580751485514	28.93583887226085
51	25.293975678632396	22.40909435231983	25.04047395485591	27.256456014191873
52	25.769269390063226	21.898391435552604	25.329384883968793	27.00295429041538
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	1.0
12	1.0
13	77.5
14	644.0
15	1134.0
16	1140.5
17	1147.0
18	1369.0
19	1591.0
20	1648.0
21	1705.0
22	1901.5
23	2098.0
24	2156.5
25	2215.0
26	2263.0
27	2311.0
28	2802.0
29	3293.0
30	3350.5
31	3408.0
32	4556.5
33	5705.0
34	7040.0
35	8375.0
36	9996.0
37	11617.0
38	11191.5
39	12588.5
40	14411.0
41	15181.0
42	15951.0
43	19103.0
44	22255.0
45	24806.5
46	27358.0
47	30594.0
48	33830.0
49	35649.0
50	37468.0
51	40766.5
52	44065.0
53	46403.5
54	48742.0
55	52557.5
56	56373.0
57	64529.5
58	72686.0
59	85628.5
60	98571.0
61	105274.5
62	111978.0
63	108280.5
64	113295.5
65	122008.0
66	102768.0
67	83528.0
68	73200.0
69	62872.0
70	50408.5
71	37945.0
72	34262.5
73	30580.0
74	26654.5
75	22729.0
76	20473.0
77	18217.0
78	13368.5
79	8520.0
80	5914.0
81	3308.0
82	2232.5
83	1157.0
84	775.0
85	393.0
86	272.0
87	151.0
88	111.5
89	42.0
90	12.0
91	10.0
92	8.0
93	6.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1135298.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.527870204395683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.4688613283853	7.547165358232057
2	11.368362932669873	2.6210602464856274
3	5.492201118674738	1.8994014489755746
4	3.2492992022044453	1.4982999783303719
5	1.9997145900185171	1.1526225119784896
6	1.3154839199264976	0.9098836730929387
7	0.9096611388936738	0.7340518877404317
8	0.6793655609065158	0.6265310405973428
9	0.5378787412411271	0.5580536683258301
>10	4.7065734242555415	13.31810268566078
>50	2.3768624353456533	19.69282969579869
>100	1.7862432895082498	35.47233771703633
>500	0.07192925998019799	5.490074756101654
>1k	0.03676384398987897	7.750449893563231
>5k	7.992139997799777E-4	0.7291354380806606
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7914	0.6970856990851741	No Hit
ACGAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3943	0.34730969313783694	No Hit
ACGAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3725	0.3281076862638708	No Hit
ACGAGCGGGATGTCTTTCTCAGTTGTGATAGCCAGAGAGAGTTAAATGGGAA	3361	0.29604561974036775	No Hit
ACGAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3299	0.2905844985193315	No Hit
ACGAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3258	0.2869731119054204	No Hit
ACGAGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2634	0.23200956929370084	No Hit
ACGAGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	2586	0.22778160447741472	No Hit
ACGAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2561	0.2255795394689324	No Hit
ACGAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2536	0.22337747446045003	No Hit
ACGAGCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2461	0.216771279435003	No Hit
ACGAGCGGGGTTCAAATGCCTCTGTTTATTCAGTACCACCTCCTGCAGCTCT	2461	0.216771279435003	No Hit
ACGAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2368	0.2085795976034486	No Hit
ACGAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2144	0.18884909512744671	No Hit
ACGAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2094	0.184444965110482	No Hit
ACGAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2006	0.17669369628062412	No Hit
ACGAGCGGCCGTTTGTGATCATCATCTTCTGTTACACGACTCTCATCCACAA	1954	0.17211340106298081	No Hit
ACGAGCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1811	0.15951758921446177	No Hit
ACGAGCGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	1767	0.1556419547995328	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1748	0.15396838539308622	No Hit
ACGAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1722	0.1516782377842646	No Hit
ACGAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1662	0.14639328176390692	No Hit
ACGAGCGGGGACTATGGGATTCAGGTTGAGCCCCCAAACTGTGAATTCAGTT	1634	0.14392696895440668	No Hit
ACGAGCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1610	0.14181298654626362	No Hit
ACGAGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1576	0.13881817813472763	No Hit
ACGAGCGGGAACTTAGCTTATGTATCTATGTATGTATGTATGTATGCATGTA	1514	0.13335705691369137	No Hit
ACGAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1513	0.13326897431335208	No Hit
ACGAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1446	0.12736744009061937	No Hit
ACGAGCGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCTGACTGCTC	1419	0.12498920988145844	No Hit
ACGAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1382	0.12173015366890455	No Hit
ACGAGCGGGGCAGATGTCTGTTCTCTGGGCTTTATGTTCTGTACAGTAGATT	1364	0.12014466686279726	No Hit
ACGAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1346	0.11855918005668996	No Hit
ACGAGCGGGGGGCATTAAAAACGTTTCCACTACAAATTTATTGTTACTGATG	1305	0.11494779344277889	No Hit
ACGAGCGGGGAGACTCCTCTCTCGCTCTCTCCGCGGCTACAGCTGGACCCTT	1238	0.1090462592200462	No Hit
ACGAGCGGGGGGTTTTTGGGTCTCTGTACACTTAATCAATTTCTAACAAACT	1228	0.10816543321665324	No Hit
ACGAGCGGGCATGGAGACTGTTTCTCAAAAAAACAAATTACCCCCTCAAAAC	1209	0.10649186381020666	No Hit
ACGAGCGGCAGTAGCTGGGGTCAGAATGGAATAAAGGGAGAAAGAACGCTGC	1165	0.10261622939527772	No Hit
ACGAGCGGGGCCGGGCAGTGGTGGCACACGCCTTTGATCCCAGCACTTGGGA	1147	0.10103074258917043	No Hit
ACGAGCGGGGACTGTCCCCATAGTAGAGGCCACAGGAAGACCGTCGTGGAGA	1146	0.10094265998883112	No Hit
ACGAGCGGGGTGCAGTGCTACGAGCGGGGTTTTCTGACAATACCAAAGAAAT	1146	0.10094265998883112	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.165782023750592E-4	0.0	0.0	0.0	0.0
8	0.00105699120407153	0.0	0.0	0.0	0.0
9	0.0011450738044108243	0.0	0.0	0.0	0.0
10	0.0023782302091609427	0.0	0.0	0.0	0.0
11	0.005461121221036239	0.0	0.0	0.0	0.0
12	0.008896342634268712	0.0	0.0	0.0	0.0
13	0.011891151045804713	0.0	0.0	0.0	0.0
14	0.01603103326175154	0.0	0.0	0.0	0.0
15	0.021404071882448486	0.0	0.0	0.0	0.0
16	0.03056466231773508	0.0	0.0	0.0	0.0
17	0.03408796633130685	0.0	0.0	0.0	0.0
18	0.03752318774453932	0.0	0.0	0.0	0.0
19	0.04465787837202215	0.0	0.0	0.0	0.0
20	0.04782885198423674	0.0	0.0	0.0	0.0
21	0.05188065159984427	0.0	0.0	0.0	0.0
22	0.05637286421714827	0.0	0.0	0.0	0.0
23	0.06139357243648804	0.0	0.0	0.0	0.0
24	0.06544537205209558	0.0	0.0	0.0	0.0
25	0.07028991507075676	0.0	0.0	0.0	0.0
26	0.07487021028840005	0.0	0.0	0.0	0.0
27	0.07795310130027534	0.0	0.0	0.0	0.0
28	0.08297380951961511	0.0	0.0	0.0	0.0
29	0.08720177433590123	0.0	0.0	0.0	0.0
30	0.09010850014709794	0.0	0.0	0.0	0.0
31	0.09697894297356288	0.0	0.0	0.0	0.0
32	0.10094265998883113	0.0	0.0	0.0	0.0
33	0.10543487260613513	0.0	0.0	0.0	0.0
34	0.11151257202954643	0.0	0.0	0.0	0.0
35	0.1211135754665295	0.0	0.0	0.0	0.0
36	0.12948142249876243	0.0	0.0	0.0	0.0
37	0.13564720452251303	0.0	0.0	0.0	0.0
38	0.13952283893744197	0.0	0.0	0.0	0.0
39	0.1467456121652641	0.0	0.0	0.0	0.0
40	0.154496880995122	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGAC	70	0.0	46.000004	10
CGCTAGT	35	1.01956175E-7	46.000004	14
AACCGTT	20	6.311423E-4	46.0	31
AGCGTAC	20	6.311423E-4	46.0	29
CGAACCC	30	1.8613828E-6	46.0	39
GACGTAA	20	6.311423E-4	46.0	9
CGTTGAT	30	1.8613828E-6	46.0	17
ATAGCCC	20	6.311423E-4	46.0	10
TAACGCG	20	6.311423E-4	46.0	20
TAACGAC	20	6.311423E-4	46.0	11
TAACGAA	20	6.311423E-4	46.0	39
AAACGAC	20	6.311423E-4	46.0	19
TCAATCG	40	5.6115823E-9	46.0	30
TAACCGT	20	6.311423E-4	46.0	30
TACGCAA	20	6.311423E-4	46.0	20
GAGGTAT	20	6.311423E-4	46.0	9
CTAAAAT	30	1.8613828E-6	46.0	35
CGAATTG	25	3.4171302E-5	46.0	13
ACGCTTA	30	1.8613828E-6	46.0	16
AATTCGC	25	3.4171302E-5	46.0	17
>>END_MODULE