FastQCFastQC Report
Fri 17 Jun 2016
SRR1527177_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527177_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1300446
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC150211.1550652622254212No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG89720.6899171515003314No Hit
GAACGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT48260.37110345220024515No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA37370.28736295086454955No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT36030.2770587936754006No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC35740.2748287895075997No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC34270.2635249752777124No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT31440.24176321046779337No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT31170.23968699969087529No Hit
GAACGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC30390.2336890574464453No Hit
GAACGCGGGATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAAC29320.2254611110342144No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA28660.22038592913508134No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG27800.21377281332712006No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG27070.20815935455989715No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC25180.19362587912147064No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC23110.17770826316509875No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC21000.1614830604269612No Hit
GAACGCGGGAACTTAGCTTATTCAACCAAATGGTATGAATCTACTTCTAT20190.155254428096207No Hit
GAACGCGGGAGTGCAGTGTTGAACGCGGGGTGCAGTGCTGAACGCGGGGG19020.14625751472956203No Hit
GAACGCGGGTGCTGTGTTAATTGAGGACGTTCCCTTCACAGAGAAAGATT18780.14441199403896818No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA18750.14418130395264392No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA17610.1354150806723232No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT17160.13195472937745975No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC16990.13064748555495576No Hit
GAACGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCA16590.12757161773729936No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG15850.121881262274635No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG15510.11926677462962705No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15510.11926677462962705No Hit
GAACGCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA14940.11488366298946669No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT14520.11165400178092748No Hit
GAACGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC14090.10834744387694684No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT13890.10680950996811862No Hit
GAACGCGGGAGGAACCTTTTTTTTTTGAAGATTTGAAACGAACTCGACTT13330.10250329502339967No Hit
GAACGCGGGACTGTCAGGGTTGTCCAGCCCACCTTGTCTCTGTCGCAGCA13250.10188812145986838No Hit
GAACGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT13200.10150363798266132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGCAC650.044.00000423
GCATAAG207.856864E-444.044
AACCGTT207.856864E-444.028
AACCGTC408.314601E-944.025
CGGGTAT1950.044.06
CAACGTA207.856864E-444.022
CCGATTT1000.044.032
CGAAAGG551.8189894E-1244.022
CGTGCTA254.4431632E-544.016
GTTTACG351.4462785E-744.025
CGCATCG454.802132E-1044.042
ACCGATT1000.044.031
GAATCGT207.856864E-444.032
TACGGTT700.044.026
TACGGTC207.856864E-444.044
TCGATCT502.7284841E-1144.013
CGTTATA207.856864E-444.037
CGTTAGT207.856864E-444.034
CGTTAGG254.4431632E-544.021
TAGCACG207.856864E-444.034
Produced by FastQC (version 0.11.5)