##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527177_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1300446
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.42200598871464	33.0	31.0	34.0	31.0	34.0
2	32.710078696078114	34.0	31.0	34.0	31.0	34.0
3	33.045903482343746	34.0	31.0	34.0	31.0	34.0
4	36.26696379549785	37.0	37.0	37.0	35.0	37.0
5	36.023978696539494	37.0	35.0	37.0	35.0	37.0
6	36.065026921533075	37.0	37.0	37.0	35.0	37.0
7	36.48371251093855	37.0	37.0	37.0	35.0	37.0
8	36.65804731607464	37.0	37.0	37.0	35.0	37.0
9	38.746503122774804	39.0	39.0	39.0	39.0	39.0
10	38.20373164283638	39.0	39.0	39.0	37.0	39.0
11	37.783416612454495	39.0	38.0	39.0	35.0	39.0
12	37.09928209245136	39.0	37.0	39.0	33.0	39.0
13	37.046231831233285	39.0	37.0	39.0	33.0	39.0
14	37.82955770558716	40.0	37.0	40.0	33.0	40.0
15	37.782562290168144	40.0	37.0	40.0	33.0	40.0
16	37.61391399565995	40.0	37.0	40.0	33.0	40.0
17	37.55416603226893	40.0	37.0	40.0	33.0	40.0
18	37.50842633988647	40.0	36.0	40.0	33.0	40.0
19	37.390529095402655	39.0	36.0	40.0	33.0	40.0
20	37.07255357008288	39.0	35.0	40.0	32.0	40.0
21	37.13519131128859	39.0	35.0	40.0	33.0	40.0
22	36.931317409565644	39.0	35.0	40.0	32.0	40.0
23	36.913867242469124	39.0	35.0	40.0	33.0	40.0
24	36.89728985286586	39.0	35.0	40.0	33.0	40.0
25	36.80728150188474	38.0	35.0	40.0	33.0	40.0
26	36.5896207916361	38.0	35.0	40.0	32.0	40.0
27	36.37345033934512	38.0	35.0	40.0	32.0	40.0
28	36.08576596029362	37.0	35.0	40.0	31.0	40.0
29	36.24156789286137	37.0	35.0	40.0	32.0	40.0
30	36.184197575293396	37.0	35.0	40.0	32.0	40.0
31	36.07132706779059	37.0	35.0	40.0	32.0	40.0
32	35.346948662228186	36.0	35.0	40.0	30.0	40.0
33	35.44795862342612	36.0	35.0	40.0	30.0	40.0
34	35.32377814995778	36.0	34.0	40.0	30.0	40.0
35	35.26915612028489	35.0	34.0	39.0	30.0	40.0
36	35.11951207508808	35.0	34.0	39.0	30.0	40.0
37	35.05014894889907	35.0	34.0	39.0	30.0	40.0
38	34.816540632982836	35.0	34.0	39.0	30.0	40.0
39	34.784132520689056	35.0	34.0	39.0	30.0	40.0
40	34.65997819209717	35.0	34.0	38.0	30.0	40.0
41	34.47572602015001	35.0	34.0	38.0	29.0	40.0
42	34.373494939428475	35.0	34.0	37.0	29.0	40.0
43	34.32600815412558	35.0	34.0	37.0	29.0	40.0
44	34.141203863905154	35.0	34.0	37.0	29.0	40.0
45	34.061947208880646	35.0	34.0	37.0	29.0	40.0
46	33.75733171542686	35.0	34.0	36.0	28.0	40.0
47	33.68996790331932	35.0	34.0	36.0	28.0	39.0
48	33.560534616585386	35.0	33.0	36.0	27.0	39.0
49	33.325542929118164	35.0	33.0	36.0	27.0	39.0
50	32.99866661130105	35.0	33.0	35.0	26.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	12.0
16	37.0
17	117.0
18	261.0
19	539.0
20	1035.0
21	1641.0
22	2263.0
23	3564.0
24	5196.0
25	6895.0
26	8969.0
27	11447.0
28	15101.0
29	19452.0
30	24921.0
31	32541.0
32	41740.0
33	60614.0
34	121074.0
35	192543.0
36	229886.0
37	227029.0
38	225004.0
39	68562.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.39551507713507	0.5385075581761949	0.027990397140673275	0.03798696754805659
8	99.52185634774531	0.4103207668753643	0.02199245489624329	0.04583043048308042
9	98.08750228767668	1.0670185459450066	0.23407354092365237	0.6114056254546517
10	58.75299704870483	31.119477471575134	3.5909987804184107	6.536526699301624
11	36.14621445258012	22.99641815192634	22.524503132002405	18.332864263491143
12	30.488463188782926	14.714413362800146	27.288945484856736	27.508177963560193
13	24.534044473972774	16.574159942050652	29.97786913105196	28.91392645292461
14	23.954781667212632	17.008549374599177	32.906249086851744	26.130419871336446
15	26.888851978475074	18.132317681779945	29.722725895577362	25.256104444167615
16	30.922929518026894	19.444021512619518	24.94105868294416	24.691990286409432
17	30.455628299829446	19.2217131660984	24.831096408462944	25.491562125609214
18	30.27161450763815	19.11705676360264	24.556344515650785	26.05498421310843
19	28.42578623026254	22.03236428117738	24.189931761872465	25.35191772668761
20	29.738643511533734	20.830468931428143	23.660113530281148	25.770774026756975
21	29.053109471673565	20.412919875181284	26.091433246747652	24.442537406397495
22	28.551819914091013	18.070262048558725	27.16337318120091	26.214544856149352
23	28.532288153448892	20.04827574539812	25.62889962366757	25.790536477485414
24	29.18498730435558	19.97045628961141	23.91987056748223	26.924685838550772
25	28.58119445174963	21.346061274362796	22.270744037045752	27.802000236841824
26	27.22266053338624	21.077307323795065	23.07339174406319	28.626640398755505
27	26.426933528958525	19.090527403675356	24.215538361454456	30.267000705911663
28	26.40009658224948	22.542112475258488	22.81701816146153	28.24077278103051
29	29.65059679525332	20.890371457176997	23.02164026803112	26.437391479538558
30	27.67596655301335	19.29384226642244	25.65888933488972	27.371301845674484
31	30.4063375180515	20.059656456323445	24.051594606773367	25.482411418851687
32	33.225524166324476	20.788252645630806	22.529347623815212	23.456875564229502
33	32.60004644560405	19.25231805088408	24.017452473997384	24.13018302951449
34	28.611337956362664	21.5895162121303	26.7548210383207	23.044324793186338
35	27.374300816796698	24.70229444359858	26.2673728859176	21.656031853687118
36	29.680970989952677	23.84951009115334	24.17862794764258	22.2908909712514
37	29.807389157258356	24.731053807693666	22.501511020065422	22.960046014982552
38	29.449511936674032	25.30585660611821	21.614430741453315	23.63020071575444
39	30.247468945269546	21.451409747117527	22.371478708074	25.929642599538926
40	28.809731430601502	21.373052014462733	21.82897252173485	27.988244033200914
41	25.670808322683143	23.164206741379495	23.368598157862763	27.7963867780746
42	24.907685517122587	21.04962451343616	24.131567170032437	29.91112279940882
43	25.659658301844136	20.245054389032685	25.93110363675231	28.164183672370864
44	25.213657468283955	20.21206570668832	24.933138323313695	29.641138501714025
45	28.193096829856835	20.711740433666602	23.254714151914037	27.840448584562527
46	26.82125978318208	22.789719834579827	23.630815889317972	26.75820449292012
47	25.79937959746118	20.994181996022903	25.728942224436846	27.47749618207907
48	27.4758813514748	22.009679756022166	24.78457390772089	25.729864984782143
49	26.76474071203264	22.030441863791346	25.300781424219075	25.904035999956935
50	25.074320656144124	21.967694160311154	25.22188541469619	27.736099768848533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.5
8	1.0
9	0.5
10	0.0
11	0.5
12	1.0
13	229.0
14	457.0
15	977.5
16	1498.0
17	2366.0
18	3234.0
19	3139.5
20	3045.0
21	3406.5
22	3768.0
23	3661.5
24	3555.0
25	3208.5
26	2862.0
27	3886.5
28	4911.0
29	4482.5
30	4054.0
31	6074.5
32	8095.0
33	10011.0
34	11927.0
35	10697.5
36	9468.0
37	13400.5
38	17333.0
39	18753.0
40	20173.0
41	22898.5
42	25624.0
43	25884.0
44	26144.0
45	31213.0
46	36282.0
47	36738.0
48	37194.0
49	42871.5
50	48549.0
51	51664.5
52	54780.0
53	59991.5
54	65203.0
55	69468.0
56	73733.0
57	77447.0
58	81161.0
59	95225.0
60	109289.0
61	115408.5
62	121528.0
63	124912.0
64	128296.0
65	131087.0
66	133878.0
67	111523.5
68	89169.0
69	73616.0
70	58063.0
71	47196.0
72	36329.0
73	33628.0
74	30927.0
75	26086.5
76	21246.0
77	19562.5
78	17879.0
79	12425.0
80	6971.0
81	4525.5
82	2080.0
83	1593.5
84	1107.0
85	712.0
86	317.0
87	287.0
88	257.0
89	149.5
90	42.0
91	26.0
92	10.0
93	8.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1300446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.79990028037635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.64808057796108	6.0409626352529795
2	11.763734921577605	2.070393884693277
3	4.855148810953446	1.281744761483344
4	2.3276101469809505	0.8193094874009802
5	1.3622897487937096	0.5994006971181797
6	0.8054359055731829	0.42526533907671793
7	0.5275081147337293	0.32494131647223007
8	0.36996281595998914	0.2604508710316106
9	0.2672936000472937	0.21169413233990855
>10	3.323468177413135	7.965400002466799
>50	2.7198449267484737	17.630272259203362
>100	2.8579171065638858	45.6263906704538
>500	0.12302585252783528	7.23810064318911
>1k	0.04690913801421057	7.6369547880883
>5k	8.850780757398222E-4	0.6987930849512838
>10k+	8.850780757398222E-4	1.1699254267781134
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	15021	1.1550652622254212	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	8972	0.6899171515003314	No Hit
GAACGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	4826	0.37110345220024515	No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3737	0.28736295086454955	No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	3603	0.2770587936754006	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3574	0.2748287895075997	No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3427	0.2635249752777124	No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3144	0.24176321046779337	No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3117	0.23968699969087529	No Hit
GAACGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC	3039	0.2336890574464453	No Hit
GAACGCGGGATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAAC	2932	0.2254611110342144	No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2866	0.22038592913508134	No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2780	0.21377281332712006	No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2707	0.20815935455989715	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2518	0.19362587912147064	No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	2311	0.17770826316509875	No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2100	0.1614830604269612	No Hit
GAACGCGGGAACTTAGCTTATTCAACCAAATGGTATGAATCTACTTCTAT	2019	0.155254428096207	No Hit
GAACGCGGGAGTGCAGTGTTGAACGCGGGGTGCAGTGCTGAACGCGGGGG	1902	0.14625751472956203	No Hit
GAACGCGGGTGCTGTGTTAATTGAGGACGTTCCCTTCACAGAGAAAGATT	1878	0.14441199403896818	No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1875	0.14418130395264392	No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	1761	0.1354150806723232	No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGT	1716	0.13195472937745975	No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1699	0.13064748555495576	No Hit
GAACGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCA	1659	0.12757161773729936	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1585	0.121881262274635	No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1551	0.11926677462962705	No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1551	0.11926677462962705	No Hit
GAACGCGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	1494	0.11488366298946669	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1452	0.11165400178092748	No Hit
GAACGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	1409	0.10834744387694684	No Hit
GAACGCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1389	0.10680950996811862	No Hit
GAACGCGGGAGGAACCTTTTTTTTTTGAAGATTTGAAACGAACTCGACTT	1333	0.10250329502339967	No Hit
GAACGCGGGACTGTCAGGGTTGTCCAGCCCACCTTGTCTCTGTCGCAGCA	1325	0.10188812145986838	No Hit
GAACGCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT	1320	0.10150363798266132	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.537933908828202E-4	0.0	0.0	0.0	0.0
8	3.075867817656404E-4	0.0	0.0	0.0	0.0
9	3.844834772070505E-4	0.0	0.0	0.0	0.0
10	0.003229661208539224	0.0	0.0	0.0	0.0
11	0.006228632330754218	0.0	0.0	0.0	0.0
12	0.00784346293502383	0.0	0.0	0.0	0.0
13	0.012380367966067026	0.0	0.0	0.0	0.0
14	0.018224516819614196	0.0	0.0	0.0	0.0
15	0.02299211193698162	0.0	0.0	0.0	0.0
16	0.03022040130847417	0.0	0.0	0.0	0.0
17	0.033680752603337624	0.0	0.0	0.0	0.0
18	0.03721800059364249	0.0	0.0	0.0	0.0
19	0.04313904614263107	0.0	0.0	0.0	0.0
20	0.04844491812808836	0.0	0.0	0.0	0.0
21	0.052289752900158866	0.0	0.0	0.0	0.0
22	0.054519757067959765	0.0	0.0	0.0	0.0
23	0.05944114557621001	0.0	0.0	0.0	0.0
24	0.06513150103887436	0.0	0.0	0.0	0.0
25	0.06966840606991755	0.0	0.0	0.0	0.0
26	0.07574324500978895	0.0	0.0	0.0	0.0
27	0.08051084012715637	0.0	0.0	0.0	0.0
28	0.08697016254423483	0.0	0.0	0.0	0.0
29	0.09081499731630532	0.0	0.0	0.0	0.0
30	0.09550569573823134	0.0	0.0	0.0	0.0
31	0.10334915867325517	0.0	0.0	0.0	0.0
32	0.10988537778577503	0.0	0.0	0.0	0.0
33	0.11549883655299797	0.0	0.0	0.0	0.0
34	0.12157367549286938	0.0	0.0	0.0	0.0
35	0.1324930062455496	0.0	0.0	7.68966954414101E-5	0.0
36	0.14379682047543688	0.0	0.0	1.537933908828202E-4	0.0
37	0.1491026924608942	0.0	0.0	1.537933908828202E-4	0.0
38	0.155254428096207	0.0	0.0	1.537933908828202E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGCAC	65	0.0	44.000004	23
GCATAAG	20	7.856864E-4	44.0	44
AACCGTT	20	7.856864E-4	44.0	28
AACCGTC	40	8.314601E-9	44.0	25
CGGGTAT	195	0.0	44.0	6
CAACGTA	20	7.856864E-4	44.0	22
CCGATTT	100	0.0	44.0	32
CGAAAGG	55	1.8189894E-12	44.0	22
CGTGCTA	25	4.4431632E-5	44.0	16
GTTTACG	35	1.4462785E-7	44.0	25
CGCATCG	45	4.802132E-10	44.0	42
ACCGATT	100	0.0	44.0	31
GAATCGT	20	7.856864E-4	44.0	32
TACGGTT	70	0.0	44.0	26
TACGGTC	20	7.856864E-4	44.0	44
TCGATCT	50	2.7284841E-11	44.0	13
CGTTATA	20	7.856864E-4	44.0	37
CGTTAGT	20	7.856864E-4	44.0	34
CGTTAGG	25	4.4431632E-5	44.0	21
TAGCACG	20	7.856864E-4	44.0	34
>>END_MODULE