FastQCFastQC Report
Fri 17 Jun 2016
SRR1527176_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527176_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392978
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC143661.031315641740214No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT85360.6127878545102651No Hit
GAACGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC51520.36985508744574574No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC36280.2604491958954126No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA35690.2562136659731884No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34140.24508642634700617No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG31530.2263495905893704No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31180.2238369880931357No Hit
GAACGCGGGATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC30140.21637096924718124No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG28830.20696665704698852No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG27910.2003621019140288No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT27250.19562405149255768No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA25760.18492754372287287No Hit
GAACGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT24190.17365672681119154No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC23630.16963656281721606No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT21800.15649924119404615No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG19340.13883923507765378No Hit
GAACGCGGGAGTGCAGTGTTGAACGCGGGGTGCAGTGCTGAACGCGGGGGGC18000.12921955694921242No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG17980.1290759796637133No Hit
GAACGCGGGAACTTAGCTTATTCAACCAAATGGTATGAATCTACTTCTATCT17500.1256301248117343No Hit
GAACGCGGGTGCTGTGTTAATTGAGGACGTTCCCTTCACAGAGAAAGATTTT17290.12412256331399346No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG17060.12247142453075353No Hit
GAACGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGC16810.12067670846201448No Hit
GAACGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC16650.11952809017802146No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA16370.11751800818103372No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15730.11292353504506172No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC15620.11213385997481654No Hit
GAACGCGGGATGTGGCTGGGTGTGTAAAGATATGTACCCCAAGGGGCATCTT14920.10710865498234716No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG14920.10710865498234716No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC14480.10394995470136643No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14400.10337564555936993No Hit
GAACGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA14110.10129377491963261No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGA253.4175308E-546.017
CGAACAC253.4175308E-546.042
CAACGTA253.4175308E-546.022
CGACGTC206.311917E-446.045
GCGAATA551.8189894E-1246.045
GTTTACG551.8189894E-1246.025
CGCATGA301.8616902E-646.037
ACCGATT900.046.031
ATTAACG301.8616902E-646.042
TACGGTT900.046.026
TAACGAC253.4175308E-546.017
TTATCGA206.311917E-446.017
CGTTATA206.311917E-446.037
AACGGTC206.311917E-446.034
AAACGAC253.4175308E-546.026
TACGCGC253.4175308E-546.015
CGGATAG206.311917E-446.06
TAACACG206.311917E-446.025
AACGCGT800.046.02
ACGGAAT253.4175308E-546.011
Produced by FastQC (version 0.11.5)