##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527176_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1392978
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37497218190094	33.0	31.0	34.0	31.0	34.0
2	32.65467509178178	34.0	31.0	34.0	31.0	34.0
3	33.03370907508948	34.0	31.0	34.0	31.0	34.0
4	36.25162708958792	37.0	37.0	37.0	35.0	37.0
5	36.06233407849945	37.0	35.0	37.0	35.0	37.0
6	36.09360449339473	37.0	37.0	37.0	35.0	37.0
7	36.49527774307993	37.0	37.0	37.0	35.0	37.0
8	36.61682452989207	37.0	37.0	37.0	35.0	37.0
9	38.70976569622779	39.0	39.0	39.0	39.0	39.0
10	38.08700568135319	39.0	39.0	39.0	37.0	39.0
11	37.67689080516706	39.0	38.0	39.0	35.0	39.0
12	37.012425896173525	39.0	37.0	39.0	33.0	39.0
13	37.000525492864924	39.0	37.0	39.0	33.0	39.0
14	37.797746985235946	40.0	37.0	40.0	33.0	40.0
15	37.65651288103617	40.0	37.0	40.0	33.0	40.0
16	32.5492391121755	39.0	33.0	40.0	3.0	40.0
17	33.80895893546057	39.0	33.0	40.0	15.0	40.0
18	35.79621932291824	39.0	33.0	40.0	27.0	40.0
19	36.81362735089858	39.0	35.0	40.0	31.0	40.0
20	37.10132464403601	39.0	35.0	40.0	33.0	40.0
21	37.22619883443959	39.0	35.0	40.0	33.0	40.0
22	37.23305608559503	39.0	35.0	40.0	33.0	40.0
23	37.12868186001502	39.0	35.0	40.0	33.0	40.0
24	37.036711276129274	39.0	35.0	40.0	33.0	40.0
25	36.93295730442261	39.0	35.0	40.0	33.0	40.0
26	36.72895982563974	38.0	35.0	40.0	33.0	40.0
27	36.52071174131968	38.0	35.0	40.0	32.0	40.0
28	36.281397839736165	37.0	35.0	40.0	32.0	40.0
29	36.03007800553921	37.0	35.0	40.0	31.0	40.0
30	35.876368471002415	37.0	35.0	40.0	31.0	40.0
31	35.74181358212405	36.0	35.0	40.0	31.0	40.0
32	35.66174196577405	36.0	35.0	40.0	31.0	40.0
33	35.245697347696805	35.0	34.0	39.0	30.0	40.0
34	35.00923417311687	35.0	34.0	39.0	29.0	40.0
35	34.87682217522459	35.0	34.0	39.0	29.0	40.0
36	34.875149499848526	35.0	34.0	39.0	30.0	40.0
37	34.57076206515824	35.0	34.0	39.0	29.0	40.0
38	34.519364986381696	35.0	34.0	38.0	29.0	40.0
39	34.16950447171455	35.0	33.0	38.0	28.0	40.0
40	34.12531784421577	35.0	33.0	37.0	28.0	40.0
41	33.99980545277815	35.0	33.0	37.0	28.0	40.0
42	33.94830499835604	35.0	33.0	37.0	28.0	40.0
43	33.920434493581375	35.0	33.0	37.0	28.0	40.0
44	34.11273114148249	35.0	34.0	37.0	29.0	40.0
45	34.21511682165835	35.0	34.0	36.0	30.0	40.0
46	34.12546716459269	35.0	34.0	36.0	30.0	39.0
47	33.8569245171137	35.0	34.0	36.0	29.0	39.0
48	33.78960471737529	35.0	34.0	36.0	29.0	39.0
49	33.75666880596822	35.0	34.0	36.0	29.0	39.0
50	33.30554323183855	35.0	33.0	35.0	27.0	39.0
51	33.37817539114042	35.0	33.0	35.0	28.0	38.0
52	33.093740173929525	35.0	33.0	35.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	33.0
16	45.0
17	115.0
18	265.0
19	541.0
20	1036.0
21	1698.0
22	2583.0
23	3975.0
24	5599.0
25	7749.0
26	10205.0
27	13426.0
28	17536.0
29	22728.0
30	30159.0
31	40041.0
32	55957.0
33	91412.0
34	157672.0
35	225817.0
36	248789.0
37	227364.0
38	181794.0
39	46435.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.41183564995283	0.5274311582810354	0.02605927731809117	0.03467391444803866
8	99.49453616640032	0.42707063571714704	0.03969911944050803	0.03869407844201416
9	98.10068787877483	1.0496217456413526	0.21637096924718124	0.63331940633664
10	58.89576145495477	31.06215604266543	3.550307327179611	6.491775175200183
11	36.256064345596265	23.039416272188074	22.393964585226758	18.3105547969889
12	30.711037790977315	14.675824025935801	27.195691532816742	27.41744665027014
13	24.58437965280141	16.40865828462474	30.06228382644952	28.944678236124332
14	24.1344802286899	17.038818990680397	32.7243502768888	26.102350503740908
15	26.980540970496303	18.133811158539476	29.602118626424822	25.283529244539398
16	29.229966302411093	27.41759022755564	22.01111575344334	21.341327716589927
17	30.653750454063168	19.095204662241617	24.698236440202216	25.552808443493007
18	30.336947173609346	19.014083495934607	24.588112662224386	26.060856668231658
19	28.52177134168666	22.01312583544033	24.12572201427445	25.339380808598555
20	29.90772287860971	20.775130691224124	23.56979076482184	25.74735566534432
21	29.11374048979955	20.321857200903388	26.107519286018878	24.456883023278188
22	28.402961137936135	17.987290538687617	27.204090804018442	26.405657519357806
23	28.694997336641354	19.890551035264018	25.600763256849714	25.813688371244915
24	29.301683156517903	19.789041894416133	23.865990704806535	27.043284244259418
25	28.75156678712801	21.163722614427506	22.3213144787642	27.763396119680284
26	27.33237710861191	21.00435182752348	22.966550799797268	28.696720264067345
27	26.462729490343712	18.997572108102208	24.118830304570498	30.420868096983583
28	26.351600671367386	22.637184506862276	22.71083965432333	28.30037516744701
29	29.940602076989016	20.835289573848257	22.77164463473221	26.452463714430525
30	27.728865782517744	19.296858959725135	25.595738051857243	27.378537205899878
31	30.515844471341257	19.951858536172143	23.9786270852806	25.553669907206
32	33.29944909395554	20.572112409528362	22.612776368327424	23.515662128188673
33	32.84947788120128	19.222055193980093	23.964556511301684	23.96391041351694
34	28.729240519232896	21.45676385413122	26.667039967608964	23.14695565902692
35	27.465760406840594	24.642384876143055	26.108165383803623	21.783689333212727
36	29.67125108939265	23.905618035604295	24.178199512124383	22.244931362878667
37	30.030481457711467	24.71324026653687	22.318801876267965	22.937476399483696
38	29.396157010376328	25.190850106749714	21.623887814452203	23.789105068421755
39	30.379302472831586	21.396102451007838	22.310617970994517	25.913977105166055
40	28.872028129661775	21.322806246760535	21.722166466376354	28.082999157201332
41	25.794090071774285	23.08758645147303	23.34990215207993	27.768421324672754
42	24.894793744050517	21.03550809847679	24.01803905015011	30.051659107322585
43	25.67700279544975	20.178351704047014	25.80966820725094	28.3349772932523
44	25.25014752566085	20.11122932307617	24.779931915651215	29.85869123561176
45	28.229160833839444	20.45768131298556	23.32255067919235	27.990607173982646
46	26.697980872634027	22.726776733013732	23.57144190360508	27.00380049074716
47	26.051093412817718	21.00277247738299	25.639026603435227	27.307107506364066
48	27.53654400859166	21.93774776055329	24.70871758204365	25.816990648811394
49	26.78922423756872	21.798621370904637	25.445269056654162	25.96688533487248
50	25.133203826621813	21.923820763859876	25.137941877043286	27.805033532475026
51	24.44640188143675	21.447646696502026	26.13731157275994	27.96863984930128
52	25.7751378700884	21.105071293301116	25.376782691471078	27.74300814513941
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.5
12	3.0
13	233.5
14	939.5
15	1415.0
16	2313.5
17	3212.0
18	3215.0
19	3218.0
20	3496.0
21	3774.0
22	3489.0
23	3204.0
24	3086.0
25	2968.0
26	3681.5
27	4395.0
28	4194.5
29	3994.0
30	5145.5
31	6297.0
32	8600.0
33	10903.0
34	10701.0
35	10499.0
36	12151.0
37	13803.0
38	14820.5
39	17753.0
40	19668.0
41	23701.5
42	27735.0
43	28316.0
44	28897.0
45	34294.0
46	39691.0
47	38041.5
48	36392.0
49	44865.5
50	53339.0
51	54393.0
52	55447.0
53	61125.5
54	66804.0
55	72358.0
56	77912.0
57	80561.5
58	83211.0
59	94879.0
60	106547.0
61	120703.5
62	134860.0
63	135602.5
64	136742.0
65	137139.0
66	117742.0
67	98345.0
68	82278.0
69	66211.0
70	55924.5
71	45638.0
72	39346.0
73	33054.0
74	29390.0
75	25726.0
76	23325.5
77	20925.0
78	15299.5
79	9674.0
80	6565.0
81	3456.0
82	2408.5
83	1361.0
84	857.5
85	354.0
86	267.0
87	180.0
88	113.0
89	34.0
90	22.0
91	14.0
92	6.0
93	4.5
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1392978.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.166064329084113
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.044247472482	7.222403900166234
2	10.433445594465544	2.1213415817467194
3	4.071674406392197	1.2417871182740534
4	2.1713732168712188	0.8829727922065248
5	1.3002104830607162	0.6609011706072385
6	0.8462785267833913	0.5161993166161494
7	0.5983874624145923	0.4258271805636903
8	0.44128690444662844	0.35889208465494554
9	0.3608862506926247	0.3301913556020879
>10	3.8058449186445817	9.839471458981382
>50	2.423001198050749	17.97917549414044
>100	2.366141032264201	42.967636710511655
>500	0.09704134960904125	6.5106263425814035
>1k	0.037906777191031735	6.780380257384004
>5k	0.0015162710876412695	1.054969024519429
>10k+	7.581355438206347E-4	1.1072242114440471
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14366	1.031315641740214	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8536	0.6127878545102651	No Hit
GAACGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	5152	0.36985508744574574	No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3628	0.2604491958954126	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3569	0.2562136659731884	No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3414	0.24508642634700617	No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3153	0.2263495905893704	No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3118	0.2238369880931357	No Hit
GAACGCGGGATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC	3014	0.21637096924718124	No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2883	0.20696665704698852	No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2791	0.2003621019140288	No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2725	0.19562405149255768	No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2576	0.18492754372287287	No Hit
GAACGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	2419	0.17365672681119154	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2363	0.16963656281721606	No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2180	0.15649924119404615	No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1934	0.13883923507765378	No Hit
GAACGCGGGAGTGCAGTGTTGAACGCGGGGTGCAGTGCTGAACGCGGGGGGC	1800	0.12921955694921242	No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1798	0.1290759796637133	No Hit
GAACGCGGGAACTTAGCTTATTCAACCAAATGGTATGAATCTACTTCTATCT	1750	0.1256301248117343	No Hit
GAACGCGGGTGCTGTGTTAATTGAGGACGTTCCCTTCACAGAGAAAGATTTT	1729	0.12412256331399346	No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1706	0.12247142453075353	No Hit
GAACGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGC	1681	0.12067670846201448	No Hit
GAACGCGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1665	0.11952809017802146	No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1637	0.11751800818103372	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1573	0.11292353504506172	No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1562	0.11213385997481654	No Hit
GAACGCGGGATGTGGCTGGGTGTGTAAAGATATGTACCCCAAGGGGCATCTT	1492	0.10710865498234716	No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1492	0.10710865498234716	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1448	0.10394995470136643	No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1440	0.10337564555936993	No Hit
GAACGCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1411	0.10129377491963261	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.1536592824868736E-4	0.0	0.0	0.0	0.0
8	2.1536592824868736E-4	0.0	0.0	0.0	0.0
9	2.871545709982498E-4	0.0	0.0	0.0	0.0
10	0.0024408138534851233	0.0	0.0	0.0	0.0
11	0.004522684493222434	0.0	0.0	0.0	0.0
12	0.005814880062714558	0.0	0.0	0.0	0.0
13	0.010122198627688305	0.0	0.0	0.0	0.0
14	0.014357728549912489	0.0	0.0	0.0	0.0
15	0.0175882174736428	0.0	0.0	0.0	0.0
16	0.02426456124935211	0.0	0.0	0.0	0.0
17	0.028212936600578043	0.0	0.0	0.0	0.0
18	0.03302277566479873	0.0	0.0	0.0	0.0
19	0.03948375351225935	0.0	0.0	0.0	0.0
20	0.04522684493222434	0.0	0.0	0.0	0.0
21	0.04982131806819634	0.0	0.0	0.0	0.0
22	0.051687822779684964	0.0	0.0	0.0	0.0
23	0.055851564059159586	0.0	0.0	0.0	0.0
24	0.061666444121874144	0.0	0.0	0.0	0.0
25	0.06733774689908957	0.0	0.0	0.0	0.0
26	0.07265010646255719	0.0	0.0	0.0	0.0
27	0.07616774995728576	0.0	0.0	0.0	0.0
28	0.08234157323374813	0.0	0.0	0.0	0.0
29	0.08585921672847668	0.0	0.0	0.0	0.0
30	0.08973580343695306	0.0	0.0	0.0	0.0
31	0.09598141535616499	0.0	0.0	0.0	0.0
32	0.10308849098837168	0.0	0.0	0.0	0.0
33	0.10804190733809148	0.0	0.0	0.0	0.0
34	0.11342605554430867	0.0	0.0	0.0	0.0
35	0.12182532674600748	0.0	0.0	0.0	0.0
36	0.1341729732989322	0.0	0.0	0.0	0.0
37	0.14063395114639285	0.0	0.0	0.0	0.0
38	0.14573094478161178	0.0	0.0	0.0	0.0
39	0.1525508658428202	0.0	0.0	0.0	0.0
40	0.15779143676353827	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	25	3.4175308E-5	46.0	17
CGAACAC	25	3.4175308E-5	46.0	42
CAACGTA	25	3.4175308E-5	46.0	22
CGACGTC	20	6.311917E-4	46.0	45
GCGAATA	55	1.8189894E-12	46.0	45
GTTTACG	55	1.8189894E-12	46.0	25
CGCATGA	30	1.8616902E-6	46.0	37
ACCGATT	90	0.0	46.0	31
ATTAACG	30	1.8616902E-6	46.0	42
TACGGTT	90	0.0	46.0	26
TAACGAC	25	3.4175308E-5	46.0	17
TTATCGA	20	6.311917E-4	46.0	17
CGTTATA	20	6.311917E-4	46.0	37
AACGGTC	20	6.311917E-4	46.0	34
AAACGAC	25	3.4175308E-5	46.0	26
TACGCGC	25	3.4175308E-5	46.0	15
CGGATAG	20	6.311917E-4	46.0	6
TAACACG	20	6.311917E-4	46.0	25
AACGCGT	80	0.0	46.0	2
ACGGAAT	25	3.4175308E-5	46.0	11
>>END_MODULE