FastQCFastQC Report
Fri 17 Jun 2016
SRR1527175_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527175_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346948
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC133530.9913523016478736No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT80770.5996519538987399No Hit
GAACGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC43500.3229523337203812No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA34990.2597724633764629No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC34360.2550952226812022No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC33430.2481907245120079No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32140.23861351737409314No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG28870.21433641090821623No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG27950.20750615465481964No Hit
GAACGCGGGATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC27800.2063925259178528No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG27330.20290315587535673No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT27300.20268043012796338No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA25850.191915352337284No Hit
GAACGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT23950.17780938833570412No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC23370.17350335721943239No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT20760.1541262171962095No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG19200.14254447833175446No Hit
GAACGCGGGAGTGCAGTGTTGAACGCGGGGTGCAGTGCTGAACGCGGGGGGC16990.12613701494044313No Hit
GAACGCGGGTGCTGTGTTAATTGAGGACGTTCCCTTCACAGAGAAAGATTTT16850.1250976281192741No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG16790.12465217662448735No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC16570.12301885447693602No Hit
GAACGCGGGAACTTAGCTTATTCAACCAAATGGTATGAATCTACTTCTATCT16330.12123704849778907No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA15960.1184900976132709No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG15930.11826737186587752No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15320.11373861500221241No Hit
GAACGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGC15300.11359013117061684No Hit
GAACGCGGGATGTGGCTGGGTGTGTAAAGATATGTACCCCAAGGGGCATCTT15190.11277347009684116No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC15180.11269922818104337No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG14380.10675987491722026No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC13680.10156294081137505No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA351.01974365E-746.00000422
TAGGTTA351.01974365E-746.00000417
CGTCTAT351.01974365E-746.00000441
CATACGT351.01974365E-746.00000430
CATACCG351.01974365E-746.00000417
TCGTTTG206.311844E-446.028
GTATTCG206.311844E-446.044
AAATACG253.417471E-546.011
GCGAATA253.417471E-546.045
TTAGCAC206.311844E-446.023
GTTTACG405.6134013E-946.025
ACCGATG206.311844E-446.036
CGATTTG301.8616429E-646.031
ATATACG900.046.032
TCTAACG301.8616429E-646.032
ACGTGCG253.417471E-546.018
CGAATAC206.311844E-446.046
AACGACA453.110472E-1046.010
CGTACTC453.110472E-1046.040
ATACGAC453.110472E-1046.013
Produced by FastQC (version 0.11.5)