##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527175_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346948
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.370251858275154	33.0	31.0	34.0	31.0	34.0
2	32.65997722258023	34.0	31.0	34.0	31.0	34.0
3	33.00569138526506	34.0	31.0	34.0	31.0	34.0
4	36.2351241473316	37.0	37.0	37.0	35.0	37.0
5	36.07763699860722	37.0	37.0	37.0	35.0	37.0
6	36.10463432886793	37.0	37.0	37.0	35.0	37.0
7	36.50181001790715	37.0	37.0	37.0	35.0	37.0
8	36.64106335211159	37.0	37.0	37.0	35.0	37.0
9	38.652020716464186	39.0	39.0	39.0	39.0	39.0
10	38.081993514226234	39.0	39.0	39.0	37.0	39.0
11	37.59828664506722	39.0	38.0	39.0	35.0	39.0
12	36.96365858221698	39.0	37.0	39.0	33.0	39.0
13	36.896049439176565	39.0	37.0	39.0	33.0	39.0
14	37.716535456454146	40.0	37.0	40.0	33.0	40.0
15	37.76887526467243	40.0	37.0	40.0	33.0	40.0
16	37.79269875303278	40.0	37.0	40.0	33.0	40.0
17	37.660174706076255	40.0	37.0	40.0	33.0	40.0
18	37.623268307314014	40.0	36.0	40.0	33.0	40.0
19	37.50519693410585	40.0	36.0	40.0	33.0	40.0
20	37.38216545850322	39.0	35.0	40.0	33.0	40.0
21	32.87156222808898	38.0	33.0	40.0	10.0	40.0
22	33.39714821953037	38.0	33.0	40.0	15.0	40.0
23	35.42730677056575	37.0	33.0	40.0	27.0	40.0
24	36.28153425373511	37.0	35.0	40.0	31.0	40.0
25	36.61120548083519	38.0	35.0	40.0	32.0	40.0
26	36.60734564363287	38.0	35.0	40.0	32.0	40.0
27	36.48887113682191	38.0	35.0	40.0	32.0	40.0
28	36.26058170025866	37.0	35.0	40.0	32.0	40.0
29	36.17359987170997	37.0	35.0	40.0	32.0	40.0
30	35.9569924005975	37.0	35.0	40.0	31.0	40.0
31	35.729037052655336	36.0	35.0	40.0	31.0	40.0
32	35.669632383729734	36.0	35.0	40.0	31.0	40.0
33	35.274876981145525	35.0	34.0	40.0	30.0	40.0
34	35.02656524231077	35.0	34.0	39.0	29.0	40.0
35	34.89851278594274	35.0	34.0	39.0	29.0	40.0
36	34.833779774720334	35.0	34.0	39.0	30.0	40.0
37	34.726554402991056	35.0	34.0	39.0	29.0	40.0
38	34.618712080941506	35.0	34.0	38.0	29.0	40.0
39	34.48316713043117	35.0	34.0	38.0	29.0	40.0
40	29.787732711285067	35.0	29.0	37.0	8.0	40.0
41	30.43148881768264	35.0	28.0	37.0	11.0	40.0
42	32.15687984985315	35.0	29.0	36.0	23.0	40.0
43	33.10734490121371	35.0	32.0	36.0	26.0	39.0
44	33.800091020588766	35.0	33.0	36.0	28.0	39.0
45	34.10554082265982	35.0	33.0	36.0	30.0	40.0
46	34.11252475967892	35.0	34.0	36.0	30.0	39.0
47	34.07872241541619	35.0	34.0	36.0	30.0	39.0
48	33.92449300195702	35.0	34.0	36.0	29.0	39.0
49	33.8338191229357	35.0	34.0	36.0	29.0	39.0
50	33.747639849496785	35.0	34.0	35.0	29.0	39.0
51	33.59050906196824	35.0	34.0	35.0	29.0	38.0
52	33.239104256437514	35.0	33.0	35.0	28.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	0.0
14	7.0
15	20.0
16	67.0
17	103.0
18	299.0
19	562.0
20	1082.0
21	1811.0
22	2735.0
23	4083.0
24	5846.0
25	8016.0
26	10730.0
27	14196.0
28	18760.0
29	24258.0
30	32390.0
31	45554.0
32	70833.0
33	94983.0
34	151851.0
35	212700.0
36	223904.0
37	195415.0
38	178563.0
39	48177.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.39374051559525	0.5450099038715674	0.02888010524533983	0.03236947528783591
8	99.53093957598958	0.4104835524459742	0.019005930444233928	0.03957094112022142
9	98.04157250317013	1.0457716259276528	0.26467242981911704	0.6479834410831005
10	58.90836171849247	30.971796981026735	3.595461740171113	6.5243795603096775
11	36.28588483000086	23.02613018468419	22.415341943415783	18.272643041899165
12	30.679580800446637	14.681190365181134	27.25487546661044	27.384353367761783
13	24.736960892328437	16.369748498086043	29.987720387127048	28.905570222458476
14	24.135230164787355	16.983877625565352	32.80371625333717	26.077175956310118
15	26.80734519818137	18.085998865583527	29.734258486593397	25.37239744964171
16	30.762063568897986	19.379293038781007	25.066372272723225	24.792271119597785
17	30.620335751640006	19.117144834099015	24.724265524727013	25.53825388953397
18	30.281941099433684	18.99375477004309	24.559893923150707	26.16441020737252
19	28.51052898849844	21.959942031912146	24.133374116892412	25.396154862697003
20	29.85943035662847	20.809043853214824	23.62251549428783	25.709010295868882
21	33.73463563552564	19.691480294710708	24.638887321559555	21.93499674820409
22	28.590116322233673	17.957189141674363	27.122724856490375	26.32996967960159
23	28.634587229796548	19.916952992988595	25.568321865432075	25.880137911782786
24	29.38725177215453	19.79044476846916	23.80366576883443	27.018637690541876
25	28.648470468050736	21.205941135069803	22.291432185949272	27.854156210930192
26	27.230672602060363	21.015881830627464	22.96339576583506	28.790049801477117
27	26.47941865610254	19.012018281329347	24.119787846301417	30.3887752162667
28	26.384240520049772	22.65172820331594	22.746312404042325	28.217718872591963
29	29.809836756875548	20.846981472187494	22.834214832346905	26.508966938590056
30	27.762615928751515	19.208610874361888	25.605591307162562	27.42318188972403
31	30.571187603381865	20.005449356619557	23.96187529139952	25.461487748599055
32	33.19742113281285	20.648458589344205	22.633761659692876	23.52035861815007
33	32.92465633417177	19.137858328606598	23.939825442407574	23.997659894814053
34	28.70563674321503	21.457918197287498	26.69813534004282	23.138309719454647
35	27.461639202107285	24.679943100995732	26.12053323513603	21.73788446176096
36	29.739678146446636	23.846057902754968	24.13649227735592	22.27777167344248
37	29.885340785241894	24.682838535711845	22.42944790741736	23.0023727716289
38	29.326224917368748	25.188203256547393	21.635876069454795	23.84969575662906
39	30.18572357655975	21.34135838948497	22.369237713705353	26.10368032024993
40	29.121317229766852	26.03545199963176	20.096544187303444	24.746686583297944
41	25.84101242215735	23.06785414136255	23.34529618069888	27.74583725578122
42	24.877946290428437	21.04312861372525	24.09046228956129	29.98846280628502
43	25.751922123200004	20.12453338955921	25.794982434362723	28.328562052878066
44	25.231857503036494	20.10174112140929	24.830876915812635	29.83552445974158
45	28.207621972043462	20.5480092772698	23.292881388145645	27.951487362541094
46	26.635846372688476	22.71750654071278	23.688665041263658	26.957982045335083
47	25.73796464303002	21.0213757323965	25.697428557004425	27.543231067569053
48	27.421919777155466	21.97612676955606	24.841122300192733	25.760831153095737
49	26.78217718872592	21.91948018780235	25.323620510962563	25.97472211250917
50	25.01611049572812	21.97456768932431	25.221834844403794	27.787486970543778
51	24.466794560740283	21.599052079219096	26.065816943193056	27.868336416847573
52	25.736108595135075	21.16770654843394	25.389398848359402	27.70678600807158
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	1.0
11	2.5
12	4.0
13	198.0
14	888.5
15	1385.0
16	2171.0
17	2957.0
18	2990.5
19	3024.0
20	3330.0
21	3636.0
22	3374.5
23	3113.0
24	3060.0
25	3007.0
26	3644.0
27	4281.0
28	4011.0
29	3741.0
30	4794.0
31	5847.0
32	8283.5
33	10720.0
34	10473.5
35	10227.0
36	11530.5
37	12834.0
38	14100.5
39	17188.0
40	19009.0
41	22834.0
42	26659.0
43	27175.5
44	27692.0
45	32926.0
46	38160.0
47	36311.0
48	34462.0
49	43143.0
50	51824.0
51	52658.0
52	53492.0
53	59194.0
54	64896.0
55	70117.5
56	75339.0
57	77679.5
58	80020.0
59	91054.0
60	102088.0
61	115999.5
62	129911.0
63	130012.5
64	132249.5
65	134385.0
66	115133.0
67	95881.0
68	80503.0
69	65125.0
70	54754.5
71	44384.0
72	38319.0
73	32254.0
74	28446.0
75	24638.0
76	22716.0
77	20794.0
78	15346.5
79	9899.0
80	6668.5
81	3438.0
82	2398.5
83	1359.0
84	842.0
85	325.0
86	253.5
87	182.0
88	118.5
89	33.0
90	11.0
91	10.5
92	10.0
93	7.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1346948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.132821461787245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.76222263973769	6.764896824005523
2	10.343696292997627	2.096216555837911
3	5.027867073730817	1.5283943817513916
4	2.983268107143661	1.2091569240932278
5	1.8966006119235717	0.9608957692468995
6	1.3227880260577296	0.8042144939899768
7	0.9079466804767667	0.6440043127065428
8	0.6933563264568493	0.5620524692310341
9	0.5649830510639285	0.5152385146829953
>10	4.6113081419989	11.184791494678414
>50	2.495409378825651	18.45344726664028
>100	2.2606577909775183	40.719626945664075
>500	0.09233562455359888	6.208872110054972
>1k	0.03599524347004702	6.649010146513419
>5k	7.825052928271091E-4	0.6404242335569944
>10k+	7.825052928271091E-4	1.0587575573463597
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	13353	0.9913523016478736	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8077	0.5996519538987399	No Hit
GAACGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	4350	0.3229523337203812	No Hit
GAACGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3499	0.2597724633764629	No Hit
GAACGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3436	0.2550952226812022	No Hit
GAACGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3343	0.2481907245120079	No Hit
GAACGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3214	0.23861351737409314	No Hit
GAACGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2887	0.21433641090821623	No Hit
GAACGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2795	0.20750615465481964	No Hit
GAACGCGGGATGCCTACTAACCAGAGCTGAACTAAGGCTGCTCCATCAACAC	2780	0.2063925259178528	No Hit
GAACGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2733	0.20290315587535673	No Hit
GAACGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2730	0.20268043012796338	No Hit
GAACGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2585	0.191915352337284	No Hit
GAACGCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	2395	0.17780938833570412	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2337	0.17350335721943239	No Hit
GAACGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	2076	0.1541262171962095	No Hit
GAACGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1920	0.14254447833175446	No Hit
GAACGCGGGAGTGCAGTGTTGAACGCGGGGTGCAGTGCTGAACGCGGGGGGC	1699	0.12613701494044313	No Hit
GAACGCGGGTGCTGTGTTAATTGAGGACGTTCCCTTCACAGAGAAAGATTTT	1685	0.1250976281192741	No Hit
GAACGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1679	0.12465217662448735	No Hit
GAACGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1657	0.12301885447693602	No Hit
GAACGCGGGAACTTAGCTTATTCAACCAAATGGTATGAATCTACTTCTATCT	1633	0.12123704849778907	No Hit
GAACGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1596	0.1184900976132709	No Hit
GAACGCGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1593	0.11826737186587752	No Hit
GAACGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1532	0.11373861500221241	No Hit
GAACGCGGGGTCAAGGAAAGTATACCCCAAGTGGCCAGTCTGGAGCCGCAGC	1530	0.11359013117061684	No Hit
GAACGCGGGATGTGGCTGGGTGTGTAAAGATATGTACCCCAAGGGGCATCTT	1519	0.11277347009684116	No Hit
GAACGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1518	0.11269922818104337	No Hit
GAACGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1438	0.10675987491722026	No Hit
GAACGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	1368	0.10156294081137505	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.424191579778878E-5	0.0	0.0	0.0	0.0
8	7.424191579778878E-5	0.0	0.0	0.0	0.0
9	7.424191579778878E-5	0.0	0.0	0.0	0.0
10	0.0023014993897314522	0.0	0.0	0.0	0.0
11	0.0035636119582938613	7.424191579778878E-5	0.0	0.0	0.0
12	0.00549390176903637	7.424191579778878E-5	0.0	0.0	0.0
13	0.009205997558925809	7.424191579778878E-5	0.0	0.0	0.0
14	0.014402931664771024	7.424191579778878E-5	0.0	0.0	0.0
15	0.017966543623064886	7.424191579778878E-5	0.0	0.0	0.0
16	0.024796799876461453	7.424191579778878E-5	0.0	0.0	0.0
17	0.028731621413744256	7.424191579778878E-5	0.0	0.0	0.0
18	0.032517959119431485	7.424191579778878E-5	0.0	0.0	0.0
19	0.03830882855165901	7.424191579778878E-5	0.0	0.0	0.0
20	0.04328303691011086	7.424191579778878E-5	0.0	0.0	0.0
21	0.04758906802638261	7.424191579778878E-5	0.0	0.0	0.0
22	0.04981632550031627	7.424191579778878E-5	0.0	0.0	0.0
23	0.05337993745861013	7.424191579778878E-5	0.0	0.0	0.0
24	0.05969050030142218	7.424191579778878E-5	0.0	0.0	0.0
25	0.06436774099668287	7.424191579778878E-5	0.0	0.0	0.0
26	0.07045557809210155	7.424191579778878E-5	0.0	0.0	0.0
27	0.07505857687156446	7.424191579778878E-5	0.0	0.0	0.0
28	0.08122065588278092	7.424191579778878E-5	0.0	0.0	0.0
29	0.08478426784107479	7.424191579778878E-5	0.0	0.0	0.0
30	0.08916454087314432	7.424191579778878E-5	0.0	0.0	0.0
31	0.0953266198843608	7.424191579778878E-5	0.0	0.0	0.0
32	0.1025280857167463	7.424191579778878E-5	0.0	0.0	0.0
33	0.10638866533823133	7.424191579778878E-5	0.0	0.0	0.0
34	0.11136287369668317	7.424191579778878E-5	0.0	0.0	0.0
35	0.12049462933981119	7.424191579778878E-5	0.0	0.0	0.0
36	0.13081425563570384	7.424191579778878E-5	0.0	0.0	0.0
37	0.1357142220783579	7.424191579778878E-5	0.0	0.0	0.0
38	0.14061418852101196	7.424191579778878E-5	0.0	0.0	0.0
39	0.1454399130478682	7.424191579778878E-5	0.0	0.0	0.0
40	0.15227016930126477	7.424191579778878E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGTA	35	1.01974365E-7	46.000004	22
TAGGTTA	35	1.01974365E-7	46.000004	17
CGTCTAT	35	1.01974365E-7	46.000004	41
CATACGT	35	1.01974365E-7	46.000004	30
CATACCG	35	1.01974365E-7	46.000004	17
TCGTTTG	20	6.311844E-4	46.0	28
GTATTCG	20	6.311844E-4	46.0	44
AAATACG	25	3.417471E-5	46.0	11
GCGAATA	25	3.417471E-5	46.0	45
TTAGCAC	20	6.311844E-4	46.0	23
GTTTACG	40	5.6134013E-9	46.0	25
ACCGATG	20	6.311844E-4	46.0	36
CGATTTG	30	1.8616429E-6	46.0	31
ATATACG	90	0.0	46.0	32
TCTAACG	30	1.8616429E-6	46.0	32
ACGTGCG	25	3.417471E-5	46.0	18
CGAATAC	20	6.311844E-4	46.0	46
AACGACA	45	3.110472E-10	46.0	10
CGTACTC	45	3.110472E-10	46.0	40
ATACGAC	45	3.110472E-10	46.0	13
>>END_MODULE