FastQCFastQC Report
Fri 17 Jun 2016
SRR1527174_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527174_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2549095
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC629462.4693469643147865No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG470661.8463807743532508No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT188250.7384973882887849No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC111110.43588018492837655No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT107160.4203844893972174No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT90270.3541256798981599No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC88640.3477312536409981No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT67310.2640544977727389No Hit
TCAAAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGC55100.2161551452574345No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49550.19438271229593249No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG48590.1906166698377267No Hit
TCAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT44850.17594479609430014No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC42390.1662943122951479No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC40130.15742842067478852No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC38910.15264240838415202No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACT37630.14762101843987768No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC35260.13832360112118222No Hit
TCAAAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG30880.12114103240561847No Hit
TCAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC29250.11474660614845661No Hit
TCAAAGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA29070.11404047318754303No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTG27660.10850909832705333No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT26420.10364462681853755No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG26230.10289926424868433No Hit
TCAAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT26160.10262465698610683No Hit
TCAAAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT26040.10215390167883111No Hit
TCAAAGGGGGTGTGAGAGATGTGAGTGTGTGTGGGGTGTATGAGTATGTG25930.10172237598049504No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC25770.10109470223746075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA351.447006E-744.013
CGGCGAA207.858289E-444.025
TCGTTCG254.444371E-544.038
CGTATGA302.5289355E-644.028
CGAAACG351.447006E-744.044
CGCATTC408.3200575E-944.043
CGCATTA207.858289E-444.012
TAGCGTA750.044.030
CGTGAAT254.444371E-544.013
ATTACGT600.044.020
CGACCGT351.447006E-744.029
ATAGACG207.858289E-444.025
TTATCGC302.5289355E-644.020
CTTATCG207.858289E-444.040
TACGACG207.858289E-444.013
TACGAAC207.858289E-444.029
ACGTGCG408.3200575E-944.039
TTACGTC207.858289E-444.030
TTACGCG207.858289E-444.032
CGGTCTA207.858289E-444.038
Produced by FastQC (version 0.11.5)