##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527174_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2549095
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.578615940167	34.0	31.0	34.0	31.0	34.0
2	32.86848155914158	34.0	31.0	34.0	31.0	34.0
3	33.01578207167642	34.0	31.0	34.0	31.0	34.0
4	36.51994492162905	37.0	37.0	37.0	35.0	37.0
5	36.35041926644554	37.0	37.0	37.0	35.0	37.0
6	36.256434538532304	37.0	37.0	37.0	35.0	37.0
7	36.607993032821454	37.0	37.0	37.0	35.0	37.0
8	36.66531965266104	37.0	37.0	37.0	35.0	37.0
9	38.72164513288049	39.0	39.0	39.0	39.0	39.0
10	38.17558662976468	39.0	39.0	39.0	37.0	39.0
11	36.72018736061229	38.0	35.0	39.0	33.0	39.0
12	36.52806074312648	39.0	35.0	39.0	33.0	39.0
13	36.44479746733645	38.0	35.0	39.0	33.0	39.0
14	37.051978054956756	39.0	35.0	40.0	33.0	40.0
15	37.02558123569345	39.0	35.0	40.0	33.0	40.0
16	36.92013008538324	39.0	35.0	40.0	33.0	40.0
17	36.65041671652096	39.0	35.0	40.0	31.0	40.0
18	36.58035891169219	39.0	35.0	40.0	31.0	40.0
19	36.51456654224342	39.0	34.0	40.0	31.0	40.0
20	36.35576783132837	39.0	34.0	40.0	31.0	40.0
21	36.66897859828684	39.0	35.0	40.0	31.0	40.0
22	36.572717376166835	39.0	35.0	40.0	31.0	40.0
23	36.78512570147444	39.0	35.0	40.0	32.0	40.0
24	36.96497188217779	39.0	35.0	40.0	33.0	40.0
25	36.95974414449049	39.0	35.0	40.0	33.0	40.0
26	36.78451293498281	38.0	35.0	40.0	33.0	40.0
27	36.715172247405455	38.0	35.0	40.0	33.0	40.0
28	36.52925646160696	38.0	35.0	40.0	32.0	40.0
29	36.75829578732844	39.0	35.0	40.0	33.0	40.0
30	36.697940249382626	39.0	35.0	40.0	32.0	40.0
31	36.679362283477076	39.0	35.0	40.0	33.0	40.0
32	36.02461344124091	38.0	35.0	40.0	31.0	40.0
33	36.201460518340824	38.0	35.0	40.0	31.0	40.0
34	35.852213824906485	38.0	34.0	40.0	30.0	40.0
35	35.934654063500965	38.0	34.0	40.0	30.0	40.0
36	36.02371351401184	38.0	35.0	40.0	31.0	40.0
37	36.22247817362633	38.0	35.0	40.0	32.0	40.0
38	36.108065019153855	38.0	35.0	40.0	32.0	40.0
39	36.09971225081843	38.0	35.0	40.0	32.0	40.0
40	36.0462936846214	37.0	35.0	40.0	31.0	40.0
41	35.948092166043246	37.0	35.0	40.0	31.0	40.0
42	35.857652617889876	37.0	35.0	40.0	31.0	40.0
43	35.80619631673202	37.0	35.0	40.0	31.0	40.0
44	35.70113942399165	36.0	35.0	40.0	31.0	40.0
45	35.57553092371999	36.0	35.0	40.0	31.0	40.0
46	35.246319183867215	36.0	35.0	40.0	30.0	40.0
47	35.190401299284645	35.0	35.0	40.0	30.0	40.0
48	35.11815369768487	35.0	34.0	40.0	30.0	40.0
49	34.88830231905833	35.0	34.0	40.0	29.0	40.0
50	34.61129812737462	35.0	34.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	6.0
14	6.0
15	41.0
16	153.0
17	348.0
18	680.0
19	1253.0
20	1824.0
21	2840.0
22	4254.0
23	6032.0
24	8585.0
25	11802.0
26	15671.0
27	20565.0
28	27091.0
29	35501.0
30	47404.0
31	63450.0
32	85214.0
33	132801.0
34	351509.0
35	255894.0
36	160881.0
37	271352.0
38	626525.0
39	417409.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.30602821785772	0.6393641664983063	0.03711121005690255	0.017496405587080908
8	99.45874908545974	0.4512581916327167	0.054058401118828446	0.035934321788713246
9	97.60342396026826	1.2306720620455494	0.5862080463850896	0.5796959313011089
10	55.848252026699676	31.740009689713407	4.537728095657478	7.874010187929442
11	43.690093935298606	16.87873539432622	22.12699016709852	17.304180503276655
12	41.56694826987617	13.262079286962628	23.932925214635	21.238047228526202
13	30.20126750866484	13.054240818800396	26.29921599626534	30.445275676269425
14	23.261078931934666	16.068251673633192	35.365139392607965	25.305530001824177
15	30.76193707963022	16.688314872533194	31.69156112267295	20.858186925163636
16	39.191987744670165	18.897138003879807	24.877534968292668	17.033339283157357
17	33.05361314505736	21.448474850878448	25.679505863845797	19.818406140218393
18	33.14533197075825	21.774119834686427	25.621995257140277	19.458552937415043
19	29.4798350002648	28.898452195779285	23.045786838073905	18.57592596588201
20	32.48399922325375	26.113346109109308	24.084155357097323	17.31849931053962
21	29.855262357817185	27.788803477312534	24.30764643922647	18.048287725643807
22	25.649926738705304	23.70264741015929	32.47838938917537	18.16903646196003
23	31.027639220978426	20.536072606160225	29.899905652790498	18.53638252007085
24	36.9714349602506	19.451020852498633	24.39367697163111	19.18386721561966
25	30.01806523491671	19.421088660877682	21.588759932446614	28.972086171759
26	23.40387470847497	20.308776251963934	28.962474917568787	27.324874121992316
27	20.681810603371	17.87657972731499	28.919675414215636	32.52193425509838
28	20.662391946945878	27.212716670033878	24.67475711968365	27.450134263336594
29	31.0660057785214	25.25068700852656	21.532661591662926	22.150645621289126
30	29.383918606407374	20.38162563576485	28.580535444932416	21.653920312895362
31	33.46505328361634	19.118706835170915	26.771893554379105	20.64434632683364
32	37.422026248531346	19.042012949693913	25.35123249623886	18.184728305535884
33	36.019018514413936	18.070217077041068	28.183139506373834	17.72762490217116
34	26.23444791190599	20.221764979335806	36.388404512189624	17.155382596568586
35	21.892122498376875	29.59301242205567	32.1036681645839	16.411196914983552
36	21.393984924061286	37.785488575357135	23.9899258364243	16.83060066415728
37	29.74181032876374	31.777670114295464	21.544822770434212	16.935696786506586
38	28.46021823431453	31.550844515406446	21.510300714567325	18.478636535711694
39	31.39471067182667	27.589987819206424	20.85999148717486	20.155310021792047
40	27.05383675382832	23.466720541996278	20.425013583252095	29.05442912092331
41	20.176690158664154	28.925795233210216	22.0079283039667	28.889586304158925
42	18.653286754710983	26.721326588455902	22.655216851470815	31.970169805362296
43	20.802441650860402	21.464519760934763	24.92347284036099	32.80956574784384
44	23.228518356514762	20.593387064821044	24.20643404816219	31.971660530502
45	26.772285850468496	24.102985569388352	22.591704114597533	26.533024465545612
46	24.44506775934204	28.394744016994267	21.758545679937388	25.401642543726304
47	21.282533605063758	23.96674113754097	26.037240667766405	28.71348458962887
48	23.448086477749946	21.10972717768463	30.878998232706117	24.563188111859308
49	28.431306012526015	20.81852579052566	26.69276743314784	24.057400763800484
50	23.289716546460607	19.301752190483292	29.267602815901327	28.140928447154774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	23.0
6	46.0
7	131.5
8	217.0
9	725.5
10	1234.0
11	3792.0
12	6350.0
13	7473.0
14	8596.0
15	8552.5
16	8509.0
17	9767.0
18	11025.0
19	12397.0
20	13769.0
21	13627.5
22	13486.0
23	14018.0
24	14550.0
25	16391.5
26	18233.0
27	20875.5
28	23518.0
29	27689.0
30	31860.0
31	33722.5
32	35585.0
33	38641.5
34	41698.0
35	47552.5
36	53407.0
37	57788.0
38	62169.0
39	63382.0
40	64595.0
41	71111.0
42	77627.0
43	81433.5
44	85240.0
45	94029.0
46	102818.0
47	104404.5
48	105991.0
49	116828.0
50	127665.0
51	135206.5
52	142748.0
53	160958.0
54	179168.0
55	204181.0
56	229194.0
57	243373.0
58	257552.0
59	261677.0
60	265802.0
61	278944.0
62	292086.0
63	209252.5
64	126419.0
65	93384.5
66	60350.0
67	47157.5
68	33965.0
69	29228.5
70	24492.0
71	19445.0
72	14398.0
73	11672.5
74	8947.0
75	6487.5
76	4028.0
77	2448.5
78	869.0
79	764.0
80	659.0
81	397.0
82	135.0
83	85.0
84	35.0
85	34.5
86	34.0
87	25.5
88	17.0
89	10.5
90	4.0
91	4.5
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2549095.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	6.801182346670462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.4931173030652	4.454306332300248
2	11.342566729877378	1.5428572921834744
3	5.036671756163081	1.0276596910197016
4	2.699737602447785	0.7344563088964124
5	1.5242603827491814	0.5183386403441447
6	0.9982067130896027	0.40733915252357705
7	0.671365724803516	0.31962565009853044
8	0.45690673095737866	0.24860047941297872
9	0.3804837997191191	0.2328965731659395
>10	4.819747248391813	8.67847292955301
>50	3.056563784906892	15.004524290777018
>100	3.2328230230446002	40.80449482855493
>500	0.20152506749533702	9.235816251678893
>1k	0.08061002699813481	9.393579453724058
>5k	0.0024062694626308897	1.2328114118321973
>10k+	0.003007836828288612	6.164220713934893
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	62946	2.4693469643147865	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	47066	1.8463807743532508	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	18825	0.7384973882887849	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	11111	0.43588018492837655	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	10716	0.4203844893972174	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	9027	0.3541256798981599	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGC	8864	0.3477312536409981	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	6731	0.2640544977727389	No Hit
TCAAAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGC	5510	0.2161551452574345	No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4955	0.19438271229593249	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTG	4859	0.1906166698377267	No Hit
TCAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4485	0.17594479609430014	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	4239	0.1662943122951479	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	4013	0.15742842067478852	No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3891	0.15264240838415202	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACT	3763	0.14762101843987768	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3526	0.13832360112118222	No Hit
TCAAAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3088	0.12114103240561847	No Hit
TCAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	2925	0.11474660614845661	No Hit
TCAAAGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA	2907	0.11404047318754303	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTG	2766	0.10850909832705333	No Hit
TCAAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2642	0.10364462681853755	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2623	0.10289926424868433	No Hit
TCAAAGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2616	0.10262465698610683	No Hit
TCAAAGGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT	2604	0.10215390167883111	No Hit
TCAAAGGGGGTGTGAGAGATGTGAGTGTGTGTGGGGTGTATGAGTATGTG	2593	0.10172237598049504	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGCAGGTAAGAGCACCCGACTGC	2577	0.10109470223746075	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.007061329609135791	0.0	0.0	0.0	0.0
6	0.007061329609135791	0.0	0.0	0.0	0.0
7	0.007139788827015078	0.0	0.0	0.0	0.0
8	0.007453625698532224	0.0	0.0	0.0	0.0
9	0.008983580447178313	0.0	0.0	0.0	0.0
10	0.017810242458598052	0.0	0.0	0.0	0.0
11	0.02459696480515634	0.0	0.0	0.0	0.0
12	0.030520635755042477	0.0	0.0	0.0	0.0
13	0.03754273575523862	0.0	0.0	0.0	0.0
14	0.05095926201259663	0.0	0.0	0.0	0.0
15	0.06029590894023173	0.0	0.0	0.0	0.0
16	0.07347705754395187	0.0	0.0	0.0	0.0
17	0.08395136313083663	0.0	0.0	0.0	0.0
18	0.0937195357568078	0.0	0.0	0.0	0.0
19	0.11215745195844015	0.0	0.0	0.0	0.0
20	0.12196485419335097	0.0	0.0	0.0	0.0
21	0.13428295140039898	0.0	0.0	0.0	0.0
22	0.14244271005984477	0.0	0.0	0.0	0.0
23	0.1489940547527652	0.0	0.0	0.0	0.0
24	0.157153813412211	0.0	0.0	0.0	0.0
25	0.16315594357997643	0.0	0.0	0.0	0.0
26	0.16696121564712182	0.0	0.0	0.0	0.0
27	0.17335564190428368	0.0	0.0	0.0	0.0
28	0.18065234916705733	0.0	0.0	0.0	0.0
29	0.18626218324542632	0.0	0.0	0.0	0.0
30	0.191244343580761	0.0	0.0	0.0	0.0
31	0.19705032570382822	0.0	0.0	0.0	0.0
32	0.20297399665371435	0.0	0.0	0.0	0.0
33	0.20811307542480761	0.0	0.0	0.0	0.0
34	0.21501748659818484	0.0	0.0	0.0	0.0
35	0.22800248715720678	0.0	0.0	0.0	0.0
36	0.2446358413476155	0.0	0.0	0.0	0.0
37	0.255580902241776	0.0	0.0	0.0	0.0
38	0.26358374246546323	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	35	1.447006E-7	44.0	13
CGGCGAA	20	7.858289E-4	44.0	25
TCGTTCG	25	4.444371E-5	44.0	38
CGTATGA	30	2.5289355E-6	44.0	28
CGAAACG	35	1.447006E-7	44.0	44
CGCATTC	40	8.3200575E-9	44.0	43
CGCATTA	20	7.858289E-4	44.0	12
TAGCGTA	75	0.0	44.0	30
CGTGAAT	25	4.444371E-5	44.0	13
ATTACGT	60	0.0	44.0	20
CGACCGT	35	1.447006E-7	44.0	29
ATAGACG	20	7.858289E-4	44.0	25
TTATCGC	30	2.5289355E-6	44.0	20
CTTATCG	20	7.858289E-4	44.0	40
TACGACG	20	7.858289E-4	44.0	13
TACGAAC	20	7.858289E-4	44.0	29
ACGTGCG	40	8.3200575E-9	44.0	39
TTACGTC	20	7.858289E-4	44.0	30
TTACGCG	20	7.858289E-4	44.0	32
CGGTCTA	20	7.858289E-4	44.0	38
>>END_MODULE