FastQCFastQC Report
Fri 17 Jun 2016
SRR1527173_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527173_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2735901
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC597232.1829371749928086No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT415061.5170870583401959No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC171380.6264115550964746No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC105940.38722161364756985No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC104790.38301824517773125No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT83160.30395836691459227No Hit
TCAAAGGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC70770.2586716405308525No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT59910.21897722176350679No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC56180.2053436875091606No Hit
TCAAAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTC52970.19361080682378493No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT49260.18005037462978374No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC48450.17708974118581045No Hit
TCAAAGGGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT48220.17624906749184271No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT41380.15124816285384596No Hit
TCAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT40250.14711789644435233No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC39740.14525379390555432No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA37860.13838220023312248No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT37500.13706636314691212No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC36200.13231472922448584No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC34360.125589339672744No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT31150.11385645898736832No Hit
TCAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT30600.1118461523278803No Hit
TCAAAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT29750.10873931476321694No Hit
TCAAAGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA28160.10292770096578788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTTCG253.4184006E-546.014
CGACGAA301.8623523E-646.010
CCGCTAA405.6170393E-946.017
ATATCGT301.8623523E-646.023
TGCGATA206.312989E-446.019
CGCTAGT206.312989E-446.014
AACGCGT206.312989E-446.037
TCGTACA206.312989E-446.035
TTACGCG253.4184006E-546.032
ACGTATT253.4184006E-546.016
ATTATCG253.4184006E-546.013
CGATAGC1650.046.033
TCGGTCG206.312989E-446.032
TACGTAT206.312989E-446.044
AGGGGAT295500.044.202035
CAAAGGG2816700.044.123552
TCAAAGG2834250.044.099461
AAAGGGG2756650.044.0334473
AAGGGGA876050.044.0309334
AAGGGGG1558300.043.8140954
Produced by FastQC (version 0.11.5)