##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2735901
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.51872819959494	33.0	31.0	34.0	31.0	34.0
2	32.80472831436518	34.0	31.0	34.0	31.0	34.0
3	32.940078606645486	34.0	31.0	34.0	31.0	34.0
4	36.45049181238649	37.0	37.0	37.0	35.0	37.0
5	36.34078828144732	37.0	37.0	37.0	35.0	37.0
6	36.26739417837122	37.0	37.0	37.0	35.0	37.0
7	36.601100697722615	37.0	37.0	37.0	35.0	37.0
8	36.62138213334474	37.0	37.0	37.0	35.0	37.0
9	38.6834117901196	39.0	39.0	39.0	38.0	39.0
10	38.05504365837799	39.0	38.0	39.0	37.0	39.0
11	36.60254629096594	38.0	35.0	39.0	33.0	39.0
12	36.414918522271094	39.0	35.0	39.0	33.0	39.0
13	36.383500718776006	38.0	35.0	39.0	33.0	39.0
14	36.99129829624683	39.0	35.0	40.0	33.0	40.0
15	36.87295629483669	39.0	35.0	40.0	33.0	40.0
16	31.96607662338659	38.0	33.0	40.0	3.0	40.0
17	33.245010327493574	38.0	32.0	40.0	17.0	40.0
18	35.13433929078574	37.0	33.0	40.0	27.0	40.0
19	36.07920681340443	38.0	34.0	40.0	30.0	40.0
20	36.47202000364779	39.0	35.0	40.0	31.0	40.0
21	36.74301189991889	39.0	35.0	40.0	32.0	40.0
22	36.83282180166607	39.0	35.0	40.0	32.0	40.0
23	36.940278906290835	39.0	35.0	40.0	33.0	40.0
24	37.039273350899755	39.0	35.0	40.0	33.0	40.0
25	37.02726231687477	39.0	35.0	40.0	33.0	40.0
26	36.89636832619309	39.0	35.0	40.0	33.0	40.0
27	36.801606856388446	38.0	35.0	40.0	33.0	40.0
28	36.692899706531776	38.0	35.0	40.0	33.0	40.0
29	36.51980426192322	38.0	35.0	40.0	32.0	40.0
30	36.37269696527762	38.0	35.0	40.0	32.0	40.0
31	36.334329348905534	38.0	35.0	40.0	32.0	40.0
32	36.31820303439342	38.0	35.0	40.0	32.0	40.0
33	35.952784110243755	38.0	35.0	40.0	31.0	40.0
34	35.389680035936976	38.0	34.0	40.0	27.0	40.0
35	35.423946992234	37.0	34.0	40.0	29.0	40.0
36	35.71818315063301	37.0	34.0	40.0	30.0	40.0
37	35.73872263652815	37.0	34.0	40.0	31.0	40.0
38	35.81331853747632	37.0	34.0	40.0	31.0	40.0
39	35.45279160320494	37.0	34.0	40.0	30.0	40.0
40	35.47672923837522	36.0	34.0	40.0	30.0	40.0
41	35.47859626499643	36.0	34.0	40.0	30.0	40.0
42	35.427275329041514	36.0	34.0	40.0	30.0	40.0
43	35.38830169658917	36.0	34.0	40.0	30.0	40.0
44	35.62367973110138	36.0	35.0	40.0	31.0	40.0
45	35.623354792443145	36.0	35.0	40.0	31.0	40.0
46	35.51504349024325	36.0	35.0	40.0	31.0	40.0
47	35.215992099129316	35.0	35.0	40.0	30.0	40.0
48	35.195751966171294	35.0	35.0	40.0	30.0	40.0
49	35.163234707688616	35.0	34.0	40.0	30.0	40.0
50	34.76266429231175	35.0	34.0	40.0	29.0	40.0
51	34.887687091016815	35.0	34.0	40.0	29.0	40.0
52	34.58941167827344	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	12.0
15	49.0
16	129.0
17	341.0
18	655.0
19	1176.0
20	2038.0
21	3210.0
22	4881.0
23	7067.0
24	10145.0
25	13833.0
26	18634.0
27	24605.0
28	32790.0
29	43778.0
30	59020.0
31	81057.0
32	117267.0
33	196795.0
34	357863.0
35	261955.0
36	225772.0
37	386993.0
38	561333.0
39	324496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.3266934731922	0.6325886791956288	0.027120864388002347	0.013596983224173681
8	99.43923409509335	0.4589712858762068	0.06663252800448555	0.03516209102595452
9	97.62250169139892	1.2214257752747633	0.578054542178244	0.5780179911480715
10	56.308689532260125	31.31907916258666	4.499212508054933	7.873018797098287
11	44.06486199610293	16.840923703014106	21.853495429841942	17.24071887104102
12	41.86255277511869	13.049668098370518	23.850643718467886	21.23713540804291
13	30.371310950213477	12.900357140115815	26.02495485033998	30.703377059330727
14	23.213705466681724	15.872211750352077	35.5118112826451	25.4022715003211
15	30.929701038158907	16.57260259051771	31.718655024432536	20.779041346890843
16	36.51670875517791	26.76237188407037	21.98171644368711	14.739202917064617
17	33.24232126820378	21.306107201978435	25.662697590300233	19.788873939517547
18	33.33326023127299	21.64870000778537	25.606811065166468	19.411228695775176
19	29.48619120355598	28.987415845821907	22.96998319749143	18.556409753130687
20	32.65352072315482	26.100980993098798	24.100872071028885	17.144626212717494
21	29.956895370117557	27.951705854853664	24.190202788770502	17.901195986258276
22	25.344045709256292	23.67947524417002	32.758897343142166	18.21758170343152
23	31.23742416118127	20.443758747118405	29.85484489387591	18.46397219782441
24	37.339435893330936	19.19506590333495	24.277084587490556	19.188413615843555
25	30.077038606294597	19.12861613048133	21.566240883716187	29.228104379507887
26	23.32748882360875	20.145794749152106	29.032958429416855	27.49375799782229
27	20.55622626695922	17.703418361994824	28.919504031761385	32.82085133928457
28	20.59858891092916	27.366889372093507	24.531808716762775	27.502713000214555
29	31.347260006849666	25.20847793834645	21.347885029465612	22.09637702533827
30	29.48827461227581	20.23377307877734	28.64566371370894	21.63228859523791
31	33.771836042312934	18.918118747717845	26.70505986875987	20.60498534120935
32	37.95327389404807	18.467115586419244	25.384617352747778	18.194993166784908
33	36.44166949023375	17.98953982618523	27.999989765711554	17.56880091786947
34	26.299562740026044	20.04677800841478	36.56250719598407	17.091152055575108
35	21.82944485198843	29.77560226046191	32.086212183847294	16.308740703702362
36	21.280009766435263	38.1336532279494	23.931896658541373	16.65444034707396
37	30.13924845964821	31.750490971712793	21.2658645177585	16.844396050880498
38	28.388563767475507	31.790185390480136	21.376065873728617	18.44518496831574
39	31.870049391407072	27.46828192979205	20.71405361524412	19.94761506355676
40	27.12473879720063	23.401139149406355	20.254460961854978	29.219661091538036
41	20.15438424124265	28.98730619273139	21.776701715449498	29.081607850576464
42	18.492153042087413	26.65202432397956	22.584442931231795	32.27137970270123
43	20.688943057515605	21.324346166034516	24.883210320841286	33.10350045560859
44	23.365136384686434	20.393427978570863	23.996847839157923	32.24458779758478
45	26.871038096773237	23.95602033845523	22.458890142589226	26.71405142218231
46	24.126201934938436	28.585208309803605	21.68835787552254	25.600231879735414
47	21.50074143764705	23.958030645114718	25.907552941425877	28.633674975812358
48	23.609662776540524	20.945019574904208	30.8472784651199	24.598039183435365
49	28.53443892889399	20.645301127489628	26.646285812242475	24.173974131373907
50	23.393390331009783	19.312650567399917	29.01570634317543	28.27825275841487
51	20.363054072497505	19.930107120104125	32.40230549277916	27.304533314619206
52	19.199744435197033	21.30387758913791	31.571281270776975	27.92509670488808
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	24.0
6	48.0
7	115.5
8	183.0
9	712.0
10	1241.0
11	3889.0
12	6537.0
13	7683.5
14	8957.0
15	9084.0
16	10363.5
17	11643.0
18	12742.0
19	13841.0
20	13079.5
21	12318.0
22	12966.0
23	13614.0
24	15487.0
25	17360.0
26	19174.0
27	20988.0
28	23985.0
29	26982.0
30	29350.0
31	31718.0
32	35455.5
33	39193.0
34	41187.5
35	43182.0
36	50245.0
37	57308.0
38	62236.5
39	68593.5
40	70022.0
41	74752.5
42	79483.0
43	85135.5
44	90788.0
45	96597.5
46	102407.0
47	106819.0
48	111231.0
49	124644.5
50	138058.0
51	148039.5
52	158021.0
53	175278.0
54	192535.0
55	221447.5
56	250360.0
57	259119.0
58	267878.0
59	273668.0
60	279458.0
61	283911.5
62	288365.0
63	215945.0
64	109800.5
65	76076.0
66	59431.0
67	42786.0
68	33882.5
69	24979.0
70	22044.0
71	19109.0
72	15289.0
73	11469.0
74	8299.0
75	5129.0
76	3534.0
77	1939.0
78	1217.0
79	495.0
80	434.0
81	373.0
82	221.5
83	70.0
84	53.5
85	37.0
86	30.5
87	24.0
88	20.5
89	16.0
90	15.0
91	16.0
92	17.0
93	8.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2735901.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.962163057029996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.17596949365453	5.507903487366102
2	10.616310451173955	1.6905758975259744
3	4.391439394096398	1.048960695325816
4	2.384373392887765	0.7593907897206493
5	1.342920583662647	0.5346276329881944
6	0.8625968268884489	0.4120881952897504
7	0.5512371811267476	0.30723282234601196
8	0.4068968216525003	0.25918230731075687
9	0.3066401724762156	0.21973671477822868
>10	4.357370738044908	9.257974034565713
>50	2.685476833912515	15.460283477721315
>100	2.6865276955165784	39.428893755409845
>500	0.15920553329581913	8.413067008348701
>1k	0.06778057358138834	9.515269235133427
>5k	0.002627154014782494	1.3512498945919638
>10k+	0.002627154014782494	5.833564051577554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	59723	2.1829371749928086	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	41506	1.5170870583401959	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	17138	0.6264115550964746	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	10594	0.38722161364756985	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	10479	0.38301824517773125	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	8316	0.30395836691459227	No Hit
TCAAAGGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7077	0.2586716405308525	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	5991	0.21897722176350679	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	5618	0.2053436875091606	No Hit
TCAAAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTC	5297	0.19361080682378493	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	4926	0.18005037462978374	No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4845	0.17708974118581045	No Hit
TCAAAGGGGGGGGCTAGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4822	0.17624906749184271	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	4138	0.15124816285384596	No Hit
TCAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4025	0.14711789644435233	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	3974	0.14525379390555432	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3786	0.13838220023312248	No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	3750	0.13706636314691212	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	3620	0.13231472922448584	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC	3436	0.125589339672744	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT	3115	0.11385645898736832	No Hit
TCAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	3060	0.1118461523278803	No Hit
TCAAAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2975	0.10873931476321694	No Hit
TCAAAGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2816	0.10292770096578788	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.005848164827601584	0.0	0.0	0.0	0.0
6	0.005848164827601584	0.0	0.0	0.0	0.0
7	0.005921266887946603	0.0	0.0	0.0	0.0
8	0.006396430280189232	0.0	0.0	0.0	0.0
9	0.007566063245709549	0.0	0.0	0.0	0.0
10	0.014985922370729057	0.0	0.0	0.0	0.0
11	0.02152855677160833	0.0	0.0	0.0	0.0
12	0.026389843784552145	0.0	0.0	0.0	0.0
13	0.03296902921560393	0.0	0.0	0.0	0.0
14	0.04733358407340032	0.0	0.0	0.0	0.0
15	0.055557565862215046	0.0	0.0	0.0	0.0
16	0.07131105986656681	0.0	0.0	0.0	0.0
17	0.08289773643125245	0.0	0.0	0.0	0.0
18	0.09254720839679506	0.0	0.0	0.0	0.0
19	0.10881241682356196	0.0	0.0	0.0	0.0
20	0.1174019089141018	0.0	0.0	0.0	0.0
21	0.13107199419862048	0.0	0.0	0.0	0.0
22	0.13969803731933284	0.0	0.0	0.0	0.0
23	0.14572895729779695	0.0	0.0	0.0	0.0
24	0.1536605308452316	0.0	0.0	0.0	0.0
25	0.1592162874314531	0.0	0.0	0.0	0.0
26	0.1632734517806017	0.0	0.0	0.0	0.0
27	0.17069331090562123	0.0	0.0	0.0	0.0
28	0.17833247621167578	0.0	0.0	0.0	0.0
29	0.18370547764703474	0.0	0.0	0.0	0.0
30	0.18926123423325625	0.0	0.0	0.0	0.0
31	0.19478043978930523	0.0	0.0	0.0	0.0
32	0.20040929843587177	0.0	0.0	0.0	0.0
33	0.20578229987123073	0.0	0.0	0.0	0.0
34	0.2119594239703849	0.0	0.0	0.0	0.0
35	0.2241309170178307	0.0	0.0	0.0	0.0
36	0.23867822702648964	0.0	0.0	0.0	0.0
37	0.2496800871084151	0.0	0.0	0.0	0.0
38	0.256697884901537	0.0	0.0	0.0	0.0
39	0.2618515801558609	0.0	0.0	0.0	0.0
40	0.2686866227981203	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATTTCG	25	3.4184006E-5	46.0	14
CGACGAA	30	1.8623523E-6	46.0	10
CCGCTAA	40	5.6170393E-9	46.0	17
ATATCGT	30	1.8623523E-6	46.0	23
TGCGATA	20	6.312989E-4	46.0	19
CGCTAGT	20	6.312989E-4	46.0	14
AACGCGT	20	6.312989E-4	46.0	37
TCGTACA	20	6.312989E-4	46.0	35
TTACGCG	25	3.4184006E-5	46.0	32
ACGTATT	25	3.4184006E-5	46.0	16
ATTATCG	25	3.4184006E-5	46.0	13
CGATAGC	165	0.0	46.0	33
TCGGTCG	20	6.312989E-4	46.0	32
TACGTAT	20	6.312989E-4	46.0	44
AGGGGAT	29550	0.0	44.20203	5
CAAAGGG	281670	0.0	44.12355	2
TCAAAGG	283425	0.0	44.09946	1
AAAGGGG	275665	0.0	44.033447	3
AAGGGGA	87605	0.0	44.030933	4
AAGGGGG	155830	0.0	43.814095	4
>>END_MODULE