FastQCFastQC Report
Fri 17 Jun 2016
SRR1527172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2657198
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC562202.115762543852585No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT411421.5483227068513525No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC172760.6501585504730923No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC101920.3835619325319378No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC98660.3712933699332906No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT76210.28680587596407947No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC57380.21594175518723108No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT55080.20728602083849226No Hit
TCAAAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTC52810.19874318737256313No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT46010.1731523206023789No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC44290.16667933665462642No Hit
TCAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT40540.15256672630342188No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT40380.15196458826177048No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA38000.143007784892206No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC37270.1402605300771715No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC36310.13664770182726316No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT36190.1361960982960246No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC33330.12543288080150594No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT30470.11466966330698727No Hit
TCAAAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT29550.11120736956749178No Hit
TCAAAGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA29490.1109815678018725No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT26940.1013849927630534No Hit
TCAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT26590.100067815796941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCG206.312956E-446.00000415
GCGATAA206.312956E-446.00000415
TCGAATA206.312956E-446.00000444
GTACGGA206.312956E-446.00000419
GTCGCTA301.8623323E-646.033
CGACGTC253.4183755E-546.020
CACGTTC253.4183755E-546.025
ATCGACG551.8189894E-1246.015
CGAAACG351.020253E-745.99999644
CGATAGC2150.044.93023333
AGGGGAT293200.044.384045
CAAAGGG2735000.044.1642072
AAGGGGA854400.044.1587074
TCAAAGG2751100.044.1289671
AAAGGGG2671800.044.0596583
ATCGTAC1150.044.037
GCGATAG2300.044.032
AAGGGGG1509350.043.7614864
AGGGGAG293250.043.701965
AGGGGGA228150.043.2579425
Produced by FastQC (version 0.11.5)