##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2657198
Sequences flagged as poor quality	0
Sequence length	52
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.495072252801634	33.0	31.0	34.0	31.0	34.0
2	32.79857805101464	34.0	31.0	34.0	31.0	34.0
3	32.91707204355867	34.0	31.0	34.0	31.0	34.0
4	36.45416186524301	37.0	37.0	37.0	35.0	37.0
5	36.35729516581	37.0	37.0	37.0	35.0	37.0
6	36.24902397186811	37.0	37.0	37.0	35.0	37.0
7	36.60312780605736	37.0	37.0	37.0	35.0	37.0
8	36.64618293405309	37.0	37.0	37.0	35.0	37.0
9	38.632275050636046	39.0	39.0	39.0	38.0	39.0
10	38.04830802973659	39.0	38.0	39.0	37.0	39.0
11	36.52431508679444	38.0	35.0	39.0	33.0	39.0
12	36.37374030840005	38.0	35.0	39.0	33.0	39.0
13	36.29156276649312	38.0	35.0	39.0	33.0	39.0
14	36.92216387337338	39.0	35.0	40.0	33.0	40.0
15	36.980865558381424	39.0	35.0	40.0	33.0	40.0
16	37.07371110470503	39.0	35.0	40.0	33.0	40.0
17	36.80850655464892	39.0	35.0	40.0	32.0	40.0
18	36.76582136521253	39.0	35.0	40.0	32.0	40.0
19	36.73284941506053	39.0	35.0	40.0	32.0	40.0
20	36.775145849123774	39.0	35.0	40.0	32.0	40.0
21	32.63411420601702	37.0	32.0	40.0	10.0	40.0
22	33.28647846340393	37.0	32.0	40.0	16.0	40.0
23	35.42007897040416	37.0	34.0	40.0	29.0	40.0
24	36.408177712010925	38.0	35.0	40.0	31.0	40.0
25	36.74968368936	38.0	35.0	40.0	33.0	40.0
26	36.79051090660162	38.0	35.0	40.0	33.0	40.0
27	36.78428555192349	38.0	35.0	40.0	33.0	40.0
28	36.68408790011132	38.0	35.0	40.0	33.0	40.0
29	36.66992297901775	38.0	35.0	40.0	33.0	40.0
30	36.457464968737746	38.0	35.0	40.0	32.0	40.0
31	36.32109011071061	38.0	35.0	40.0	32.0	40.0
32	36.33235536079735	38.0	35.0	40.0	32.0	40.0
33	35.99270848465188	38.0	35.0	40.0	31.0	40.0
34	35.42405119979768	38.0	34.0	40.0	27.0	40.0
35	35.450419953650425	38.0	34.0	40.0	29.0	40.0
36	35.67375709299796	37.0	34.0	40.0	30.0	40.0
37	35.901834187742125	37.0	34.0	40.0	31.0	40.0
38	35.90970488461906	37.0	34.0	40.0	31.0	40.0
39	35.76111377473564	37.0	34.0	40.0	31.0	40.0
40	31.001208792118614	35.0	31.0	40.0	8.0	40.0
41	31.84847534884491	35.0	30.0	40.0	13.0	40.0
42	33.65957410776314	35.0	31.0	40.0	23.0	40.0
43	34.637558811951536	35.0	33.0	40.0	28.0	40.0
44	35.33181682358635	36.0	34.0	40.0	30.0	40.0
45	35.50906481188078	36.0	35.0	40.0	31.0	40.0
46	35.494359848231106	36.0	35.0	40.0	31.0	40.0
47	35.43822552929816	36.0	35.0	40.0	31.0	40.0
48	35.31846742320294	35.0	35.0	40.0	31.0	40.0
49	35.22459447884576	35.0	35.0	40.0	30.0	40.0
50	35.202300694189894	35.0	34.0	40.0	30.0	40.0
51	35.08247635291009	35.0	34.0	40.0	30.0	40.0
52	34.73080666175422	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	13.0
15	40.0
16	143.0
17	379.0
18	720.0
19	1291.0
20	2122.0
21	3293.0
22	5106.0
23	7340.0
24	10472.0
25	14416.0
26	19485.0
27	25741.0
28	34815.0
29	47150.0
30	64964.0
31	96495.0
32	142584.0
33	141546.0
34	366509.0
35	297073.0
36	260050.0
37	247359.0
38	537465.0
39	330626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.32933112248315	0.6319438747131377	0.02739728089513841	0.011327721908566844
8	99.46033378016995	0.45792598067588497	0.04926241853260465	0.03247782062157205
9	97.5725933859652	1.2328776402812285	0.6063530079429534	0.5881759658106019
10	56.3303524991363	31.297818228073332	4.487509022662218	7.884320250128142
11	44.08391847351985	16.844774081570137	21.884481322054285	17.18682612285573
12	41.88460927638813	13.090217590108077	23.838268732702645	21.186904400801147
13	30.479738431234704	12.953983858184447	26.049884126060608	30.516393584520234
14	23.25171101287898	15.909503168375108	35.522870331830745	25.315915486915163
15	30.968034749386387	16.474271017816513	31.737416632106452	20.820277600690655
16	39.386526709714516	18.711213842551437	24.926294540339107	16.975964907394932
17	33.1989938273324	21.286031375908003	25.684499235661022	19.83047556109857
18	33.33044808855042	21.661050474974015	25.596022577165872	19.412478859309694
19	29.550338363945784	28.925770680242874	22.99346906026574	18.530421895545608
20	32.64107529811478	26.18581678896341	24.044501011968247	17.12860690095356
21	34.39882914257801	26.057674286974475	23.05138721314708	16.492109357300436
22	25.455611512578287	23.6761054313604	32.63264536553166	18.23563769052965
23	31.190976359307815	20.501144438615412	29.8797831399843	18.428096062092475
24	37.39747659000195	19.191644732534044	24.240760379918996	19.17011829754501
25	30.093880847418973	19.107420673958057	21.60979347417844	29.188905004444532
26	23.31693008951535	20.145958261296297	29.018838641305617	27.518273007882737
27	20.553267012845865	17.787835155679026	28.888137052639664	32.77076077883545
28	20.613970054169844	27.322540510718436	24.52387816037796	27.539611274733762
29	31.16425648370953	25.25476084205994	21.35230419411726	22.228678480113263
30	29.515489624785207	20.262434338728237	28.582627263756784	21.639448772729768
31	33.74648784170393	19.010626983762595	26.694886869552064	20.547998304981412
32	37.913885227973225	18.482250852213497	25.343764371341543	18.260099548471736
33	36.40112629920691	18.040130995130962	27.958736985350736	17.600005720311394
34	26.35181872032118	20.094814161383532	36.460135827288745	17.093231291006543
35	21.88455658930949	29.792811826593272	32.050829482786	16.27180210131123
36	21.385835756311725	38.16049838965708	23.87514968775379	16.57851616627741
37	30.01947916564742	31.85829584396797	21.278843353035793	16.843381637348816
38	28.36288451218163	31.727443720791598	21.364986726619545	18.544685040407227
39	31.61190848404974	27.552519609001664	20.705946640032096	20.129625266916502
40	27.763606626228075	27.726499869411313	18.797996987804446	25.71189651655616
41	20.166092252064015	28.987075859608506	21.785693049595853	29.061138838731626
42	18.532040141532548	26.70493504812212	22.542919270600084	32.220105539745255
43	20.715769016836532	21.365889933682023	24.835973834091398	33.08236721539004
44	23.310118402919166	20.460650655314357	23.9654703940015	32.263760547764974
45	26.867324151229983	24.001448141990174	22.396148122947558	26.735079583832295
46	24.11224154165403	28.569267325957643	21.693904631871618	25.62458650051671
47	21.2847894661971	24.01962518412252	25.91071497118393	28.784870378496446
48	23.57027967054017	21.01356391206075	30.7783988998938	24.637757517505282
49	28.53694003984649	20.679603100709844	26.64743086514441	24.13602599429926
50	23.28979624401343	19.208391696817475	29.215399078277194	28.2864129808919
51	20.38922203012346	20.01954690617711	32.34982865409352	27.241402409605907
52	19.21644529312456	21.311810410816207	31.528775800674243	27.94296849538499
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	23.0
6	44.0
7	109.0
8	174.0
9	684.5
10	1195.0
11	3609.0
12	6023.0
13	7190.0
14	8442.0
15	8527.0
16	9718.0
17	10909.0
18	11920.0
19	12931.0
20	12720.5
21	12510.0
22	12875.5
23	13241.0
24	15089.5
25	16938.0
26	18705.5
27	20473.0
28	23299.5
29	26126.0
30	28232.5
31	30339.0
32	34088.5
33	37838.0
34	39884.0
35	41930.0
36	48404.0
37	54878.0
38	59707.0
39	65725.0
40	66914.0
41	71559.5
42	76205.0
43	82303.0
44	88401.0
45	93628.0
46	98855.0
47	103334.5
48	107814.0
49	121175.0
50	134536.0
51	143607.0
52	152678.0
53	167939.5
54	183201.0
55	212825.0
56	242449.0
57	250070.5
58	257692.0
59	262256.5
60	266821.0
61	277167.0
62	287513.0
63	216253.5
64	110325.0
65	75656.0
66	59939.5
67	44223.0
68	34498.5
69	24774.0
70	21644.5
71	18515.0
72	14763.5
73	11012.0
74	7970.0
75	4928.0
76	3498.0
77	2068.0
78	1283.0
79	498.0
80	425.0
81	352.0
82	200.5
83	49.0
84	36.0
85	23.0
86	23.5
87	24.0
88	18.5
89	11.0
90	9.0
91	9.5
92	10.0
93	5.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2657198.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.17634727461908
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.608274311239	5.691414239556363
2	10.503428478310958	1.7175935762558845
3	4.349528692840759	1.0668977122075811
4	2.2034212024590127	0.7206374777425458
5	1.2975192034043874	0.5304483801260692
6	0.7799830150497276	0.3826447199610607
7	0.5376968645133199	0.3077477404914295
8	0.39514624404841414	0.25846823324809737
9	0.29246077177454627	0.21521347508086236
>10	4.502805393914953	9.893397137553423
>50	2.7289117485573877	16.104161870215798
>100	2.5824038021741753	38.96009097227114
>500	0.1497279012482533	8.157171416287692
>1k	0.06386243816681772	9.02417885580888
>5k	0.002683295721294862	1.4925042283452459
>10k+	0.00214663657703589	5.477429964847917
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	56220	2.115762543852585	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	41142	1.5483227068513525	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	17276	0.6501585504730923	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	10192	0.3835619325319378	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	9866	0.3712933699332906	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	7621	0.28680587596407947	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTC	5738	0.21594175518723108	No Hit
TCAAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	5508	0.20728602083849226	No Hit
TCAAAGGGGGGAGCTGAAGAGATGGCTGAGCGGTTAAGAGCACTGACTGCTC	5281	0.19874318737256313	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCT	4601	0.1731523206023789	No Hit
TCAAAGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4429	0.16667933665462642	No Hit
TCAAAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4054	0.15256672630342188	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	4038	0.15196458826177048	No Hit
TCAAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3800	0.143007784892206	No Hit
TCAAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	3727	0.1402605300771715	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	3631	0.13664770182726316	No Hit
TCAAAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	3619	0.1361960982960246	No Hit
TCAAAGGGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC	3333	0.12543288080150594	No Hit
TCAAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTT	3047	0.11466966330698727	No Hit
TCAAAGGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2955	0.11120736956749178	No Hit
TCAAAGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA	2949	0.1109815678018725	No Hit
TCAAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGTACCCGACTGCT	2694	0.1013849927630534	No Hit
TCAAAGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCTT	2659	0.100067815796941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.007827794541468118	0.0	0.0	0.0	0.0
6	0.007827794541468118	0.0	0.0	0.0	0.0
7	0.007940695424277755	0.0	0.0	0.0	0.0
8	0.008241764445103451	0.0	0.0	0.0	0.0
9	0.009220238762786966	0.0	0.0	0.0	0.0
10	0.01738673595268399	0.0	0.0	0.0	0.0
11	0.023370482741594718	0.0	0.0	0.0	0.0
12	0.029580031296124715	0.0	0.0	0.0	0.0
13	0.035902480733464345	0.0	0.0	0.0	0.0
14	0.04952585392582713	0.0	0.0	0.0	0.0
15	0.05863319180580446	0.0	0.0	0.0	0.0
16	0.07188022872213512	0.0	0.0	0.0	0.0
17	0.08094993297450924	0.0	0.0	0.0	0.0
18	0.08949276644043838	0.0	0.0	0.0	0.0
19	0.10541179091659711	0.0	0.0	0.0	0.0
20	0.1147072969345905	0.0	0.0	0.0	0.0
21	0.12607265247076055	0.0	0.0	0.0	0.0
22	0.1346154859366897	0.0	0.0	0.0	0.0
23	0.1412013707672518	0.0	0.0	0.0	0.0
24	0.15023344139202272	0.0	0.0	0.0	0.0
25	0.15580321827729812	0.0	0.0	0.0	0.0
26	0.16058268898290606	0.0	0.0	0.0	0.0
27	0.16777071185511958	0.0	0.0	0.0	0.0
28	0.1746952993341106	0.0	0.0	0.0	0.0
29	0.18007690808136992	0.0	0.0	0.0	0.0
30	0.18444240888334254	0.0	0.0	0.0	0.0
31	0.1886950088025055	0.0	0.0	0.0	0.0
32	0.19456585470860657	0.0	0.0	0.0	0.0
33	0.1993453254142145	0.0	0.0	0.0	0.0
34	0.20536670583072847	0.0	0.0	0.0	0.0
35	0.21695786313251778	0.0	0.0	0.0	0.0
36	0.23295215486388293	0.0	0.0	0.0	0.0
37	0.24168315646782815	0.0	0.0	0.0	0.0
38	0.24905934747805772	0.0	0.0	0.0	0.0
39	0.25474202524614276	0.0	0.0	0.0	0.0
40	0.26159134546992735	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	6.312956E-4	46.000004	15
GCGATAA	20	6.312956E-4	46.000004	15
TCGAATA	20	6.312956E-4	46.000004	44
GTACGGA	20	6.312956E-4	46.000004	19
GTCGCTA	30	1.8623323E-6	46.0	33
CGACGTC	25	3.4183755E-5	46.0	20
CACGTTC	25	3.4183755E-5	46.0	25
ATCGACG	55	1.8189894E-12	46.0	15
CGAAACG	35	1.020253E-7	45.999996	44
CGATAGC	215	0.0	44.930233	33
AGGGGAT	29320	0.0	44.38404	5
CAAAGGG	273500	0.0	44.164207	2
AAGGGGA	85440	0.0	44.158707	4
TCAAAGG	275110	0.0	44.128967	1
AAAGGGG	267180	0.0	44.059658	3
ATCGTAC	115	0.0	44.0	37
GCGATAG	230	0.0	44.0	32
AAGGGGG	150935	0.0	43.761486	4
AGGGGAG	29325	0.0	43.70196	5
AGGGGGA	22815	0.0	43.257942	5
>>END_MODULE