FastQCFastQC Report
Fri 17 Jun 2016
SRR1527171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1026448
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGATCAACAGA30600.29811544276962887No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC29840.2907112683740433No Hit
AACGCCGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTG27360.2665502782410799No Hit
AACGCCGGGACCTTTGTATTATTGTGATAGCTTGTTGCTTATTATTTTAT21780.21218805044191227No Hit
AACGCCGGAGATGAATTTCTCATGTGGTCCATATCTCAACATTTGCTTTT17440.16990631770922637No Hit
AACGCCGGGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGA16510.16084594640936511No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG16430.16006655963088243No Hit
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15890.15480569887612428No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA14070.1370746496656431No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC13570.13220348230012627No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC12900.12567611803033374No Hit
AACGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12750.12421476782067868No Hit
AACGCCGGGATCACCTTTGGAAAATGTTTATTCCAAACTAGCTTTAATGG12580.12255857091640297No Hit
AACGCCGGGACCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGAC11530.11232911944881767No Hit
AACGCCGGGATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTT11500.11203684940688666No Hit
AACGCCGGGAACATGAACTGTGTTAGTGAGCACACTGCTTCAAATAAGGT11050.10765279877792153No Hit
AACGCCGGGGTTCAGTTACAGTGTACTCATATGTATAAAATAAATAAATC10470.10200224463392203No Hit
AACGCCGGGGCTATTTGTAGTTACTTTTGCTTTCTTGAGAGACTGCAGAT10380.101125434508129No Hit
AACGCCGGGGCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG10340.10073574111888765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGCT650.044.00000429
CTATGCG408.310963E-944.010
TCGTTAT254.442506E-544.027
TACCCGA302.5274512E-644.029
ACACGAT502.7284841E-1144.036
GGTAATC454.802132E-1044.09
GTATTCG454.802132E-1044.027
TTAATCG207.8560883E-444.022
ATAGGTA302.5274512E-644.034
CGAGTAC254.442506E-544.042
TAGGTCG750.044.019
GACGTAT207.8560883E-444.025
CTATAAC207.8560883E-444.033
CGCATAC207.8560883E-444.017
TCTTATC207.8560883E-444.013
TAGCGAA254.442506E-544.041
ATTCGTT254.442506E-544.033
TATATCG207.8560883E-444.033
CGATTTC302.5274512E-644.028
ATTCGAA302.5274512E-644.010
Produced by FastQC (version 0.11.5)