##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1026448
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2273617367855	33.0	31.0	34.0	31.0	34.0
2	32.88387721540692	34.0	31.0	34.0	31.0	34.0
3	32.58187068414571	34.0	31.0	34.0	31.0	34.0
4	36.190249286861096	37.0	37.0	37.0	35.0	37.0
5	36.22250615715555	37.0	37.0	37.0	35.0	37.0
6	36.37094718875189	37.0	37.0	37.0	35.0	37.0
7	36.47844995557495	37.0	37.0	37.0	35.0	37.0
8	36.62951946908173	37.0	37.0	37.0	35.0	37.0
9	38.69782102941406	39.0	39.0	39.0	39.0	39.0
10	38.14056045703241	39.0	39.0	39.0	37.0	39.0
11	37.802384533848766	39.0	38.0	39.0	35.0	39.0
12	37.42294300344489	39.0	37.0	39.0	35.0	39.0
13	37.2961026764142	39.0	37.0	39.0	35.0	39.0
14	38.17164727292566	40.0	38.0	40.0	35.0	40.0
15	38.10454109706483	40.0	38.0	40.0	34.0	40.0
16	37.98452527551323	40.0	38.0	40.0	34.0	40.0
17	38.040086784717786	40.0	38.0	40.0	34.0	40.0
18	38.02314096768662	40.0	38.0	40.0	34.0	40.0
19	37.965883318005396	40.0	38.0	40.0	34.0	40.0
20	37.71467721696569	40.0	38.0	40.0	33.0	40.0
21	37.80326134397456	40.0	38.0	40.0	33.0	40.0
22	37.63874351160507	40.0	37.0	40.0	33.0	40.0
23	37.63665670350568	40.0	37.0	40.0	33.0	40.0
24	37.6107362477203	40.0	37.0	40.0	33.0	40.0
25	37.54128119495581	40.0	37.0	40.0	33.0	40.0
26	37.36044008074447	39.0	36.0	40.0	33.0	40.0
27	37.183860263744485	39.0	36.0	40.0	33.0	40.0
28	36.94639280314249	39.0	35.0	40.0	32.0	40.0
29	37.250302012376665	39.0	36.0	40.0	33.0	40.0
30	37.2348993811669	39.0	35.0	40.0	33.0	40.0
31	37.161618513553535	39.0	35.0	40.0	33.0	40.0
32	36.37460738391034	39.0	35.0	40.0	31.0	40.0
33	36.53878131186382	39.0	35.0	40.0	31.0	40.0
34	36.52317896279207	39.0	35.0	40.0	31.0	40.0
35	36.466060628497495	39.0	35.0	40.0	31.0	40.0
36	36.219290212460834	38.0	35.0	40.0	31.0	40.0
37	36.14119565725687	38.0	35.0	40.0	31.0	40.0
38	35.91046891805527	38.0	35.0	40.0	30.0	40.0
39	35.86609453182236	37.0	35.0	40.0	31.0	40.0
40	35.755808379966645	37.0	35.0	40.0	30.0	40.0
41	35.58246009539694	37.0	35.0	40.0	30.0	40.0
42	35.421020840802456	37.0	35.0	40.0	30.0	40.0
43	35.21116023412779	36.0	34.0	40.0	29.0	40.0
44	35.03034347575328	36.0	34.0	40.0	29.0	40.0
45	34.911593183483234	35.0	34.0	40.0	29.0	40.0
46	34.59816766168379	35.0	34.0	39.0	28.0	40.0
47	34.51348631396817	35.0	34.0	39.0	28.0	40.0
48	34.37320059077518	35.0	34.0	39.0	28.0	40.0
49	34.09836348261188	35.0	34.0	39.0	27.0	40.0
50	33.66931593222453	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	1.0
13	1.0
14	5.0
15	9.0
16	33.0
17	92.0
18	151.0
19	299.0
20	598.0
21	914.0
22	1421.0
23	2189.0
24	3264.0
25	4624.0
26	6087.0
27	8155.0
28	10670.0
29	13878.0
30	17424.0
31	22089.0
32	28209.0
33	38399.0
34	63662.0
35	90507.0
36	140985.0
37	195393.0
38	275326.0
39	102059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.1686865774009	0.4069373217152744	0.11028322915530062	0.314092871728524
8	99.12114398391346	0.5872679375867068	0.13356740916247095	0.15802066933736536
9	95.81196514582327	2.2686000654684895	0.8453423846117876	1.074092404096457
10	49.52837357567066	36.01205321652923	5.721380917494115	8.738192290305987
11	30.73131809891977	23.252127725905257	26.424426761024428	19.592127414150546
12	28.205910089941234	15.921507957539008	28.008238118248563	27.864343834271192
13	22.72535968699827	18.191764219911775	32.56755334902498	26.515322744064967
14	24.069022493102427	19.128587127647968	31.054861035337396	25.747529343912213
15	24.3081968107493	21.547706264710925	28.318531479431986	25.82556544510779
16	28.260662011129646	21.533872149392856	25.92220940563964	24.283256433837856
17	28.090755693420416	21.00759122722242	24.876077502221253	26.02557557713591
18	25.904478356429163	22.168390410425076	24.513565226879493	27.413566006266272
19	26.953240690224934	20.043879475628575	25.930977506897573	27.07190232724892
20	27.90428740666843	20.413893348713234	25.827319065359376	25.85450017925896
21	27.423698034386547	20.244084454351317	26.890889747946318	25.441327763315826
22	28.460672143157762	20.533821489252258	25.19445700123143	25.811049366358553
23	26.28891088491575	21.698127912958086	25.51322619363085	26.499735008495318
24	27.047449067074027	23.27667840942746	24.337618661637023	25.33825386186149
25	26.109457079170106	24.699741243589543	23.393099309463313	25.79770236777703
26	26.91407650460618	23.52822549218275	23.502310881798202	26.05538712141287
27	27.021047339952926	20.45588281140399	24.995615949371036	27.527453899272054
28	25.9972253830686	21.923857808676132	24.87753885243091	27.201377955824356
29	28.12115177778124	21.130636914875375	24.753324084610227	25.994887222733155
30	26.84890028525556	21.791946596417937	23.165128676757128	28.194024441569372
31	27.82206210153851	21.596028244976853	23.899603292129754	26.682306361354886
32	27.726879491216312	22.521160351035803	24.895269901641388	24.856690256106496
33	27.800531541782924	22.014558945022056	25.042963696163856	25.141945817031157
34	27.65449394416473	22.97992689352018	25.583955543778153	23.781623618536933
35	26.63106168066965	24.236493227128893	25.757271678643246	23.375173413558212
36	28.616744345548923	23.721708259941078	24.247794335416895	23.413753059093107
37	26.974868673327823	25.17847957227254	23.112325222514926	24.73432653188471
38	26.64304475238882	24.546007201533833	24.103120664661045	24.7078273814163
39	27.269574298941592	23.317985908687046	23.230986859538916	26.18145293283245
40	26.632912724268547	25.752595357972346	23.569630414789643	24.044861502969464
41	26.281409287172853	24.256659860022133	23.40703084812869	26.05490000467632
42	26.77203326422771	23.223874955185263	23.66140320795598	26.34268857263105
43	25.49549514442037	22.912022820444875	26.15290789207052	25.439574143064235
44	24.90092045578539	22.76530325939551	26.6315487974062	25.702227487412905
45	25.563983757579535	22.291728368119962	25.741099402989725	26.403188471310774
46	26.745631537106608	23.395632316493383	25.01714650912662	24.841589637273394
47	25.575382289214843	23.236539990335604	26.102442598163766	25.085635122285787
48	26.44936713793587	23.324708119651458	25.260120337318597	24.965804405094072
49	25.31798980562094	23.82380792792231	26.658242794569233	24.19995947188752
50	24.6993515502003	24.08753292909139	25.021238289713654	26.191877230994653
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	19.0
12	35.0
13	313.5
14	592.0
15	1555.0
16	2518.0
17	3603.0
18	4688.0
19	4337.0
20	3986.0
21	3930.0
22	3874.0
23	3821.5
24	3769.0
25	4325.0
26	4881.0
27	5276.0
28	5671.0
29	5874.0
30	6077.0
31	7422.0
32	8767.0
33	11362.0
34	13957.0
35	12425.0
36	10893.0
37	12547.5
38	14202.0
39	16647.0
40	19092.0
41	22240.5
42	25389.0
43	30622.0
44	35855.0
45	36694.0
46	37533.0
47	35972.5
48	34412.0
49	39480.0
50	44548.0
51	45787.0
52	47026.0
53	48885.0
54	50744.0
55	56480.0
56	62216.0
57	67043.0
58	71870.0
59	74430.5
60	76991.0
61	82775.5
62	88560.0
63	88164.5
64	87769.0
65	78870.0
66	69971.0
67	65300.0
68	60629.0
69	50321.0
70	40013.0
71	34772.0
72	29531.0
73	25758.5
74	21986.0
75	19235.0
76	16484.0
77	14855.5
78	13227.0
79	9332.5
80	5438.0
81	3774.0
82	2110.0
83	1526.0
84	942.0
85	534.5
86	127.0
87	95.5
88	64.0
89	34.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1026448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.875904723296319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.78281615765319	6.792925389729895
2	11.958505107709332	2.362021141535795
3	4.69987587835933	1.3924657915798602
4	2.250918258575862	0.8891941704649313
5	1.2883610521981943	0.6361865500357582
6	0.7746150814630837	0.4590014845054698
7	0.5077476605444462	0.3510127263309487
8	0.3258380643361639	0.25743565428858006
9	0.27286439123124306	0.24253044571620075
>10	3.5632291381788	9.663214482288168
>50	2.7626270232610177	19.80398789675123
>100	2.693661298005949	47.48977108418867
>500	0.09995032645662147	6.629955196050368
>1k	0.01899056202675808	3.030297986534113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACGCCGGGAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGATCAACAGA	3060	0.29811544276962887	No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2984	0.2907112683740433	No Hit
AACGCCGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTG	2736	0.2665502782410799	No Hit
AACGCCGGGACCTTTGTATTATTGTGATAGCTTGTTGCTTATTATTTTAT	2178	0.21218805044191227	No Hit
AACGCCGGAGATGAATTTCTCATGTGGTCCATATCTCAACATTTGCTTTT	1744	0.16990631770922637	No Hit
AACGCCGGGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGA	1651	0.16084594640936511	No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1643	0.16006655963088243	No Hit
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1589	0.15480569887612428	No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA	1407	0.1370746496656431	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1357	0.13220348230012627	No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1290	0.12567611803033374	No Hit
AACGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1275	0.12421476782067868	No Hit
AACGCCGGGATCACCTTTGGAAAATGTTTATTCCAAACTAGCTTTAATGG	1258	0.12255857091640297	No Hit
AACGCCGGGACCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGAC	1153	0.11232911944881767	No Hit
AACGCCGGGATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTT	1150	0.11203684940688666	No Hit
AACGCCGGGAACATGAACTGTGTTAGTGAGCACACTGCTTCAAATAAGGT	1105	0.10765279877792153	No Hit
AACGCCGGGGTTCAGTTACAGTGTACTCATATGTATAAAATAAATAAATC	1047	0.10200224463392203	No Hit
AACGCCGGGGCTATTTGTAGTTACTTTTGCTTTCTTGAGAGACTGCAGAT	1038	0.101125434508129	No Hit
AACGCCGGGGCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG	1034	0.10073574111888765	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.922700419310087E-4	0.0	0.0	0.0	0.0
8	2.922700419310087E-4	0.0	0.0	0.0	0.0
9	3.896933892413449E-4	0.0	0.0	0.0	0.0
10	0.003409817155861768	0.0	0.0	0.0	0.0
11	0.005553130796689165	0.0	0.0	0.0	0.0
12	0.008962947952550933	0.0	0.0	0.0	0.0
13	0.013736691970757407	0.0	0.0	0.0	0.0
14	0.023868720091032376	0.0	0.0	0.0	0.0
15	0.030493507708135238	0.0	0.0	0.0	0.0
16	0.04111265256496189	0.0	0.0	0.0	0.0
17	0.04871167365516811	0.0	0.0	0.0	0.0
18	0.05387511106261594	0.0	0.0	0.0	0.0
19	0.06410456253020123	0.0	0.0	0.0	0.0
20	0.06819634311723535	0.0	0.0	0.0	0.0
21	0.07462628403971755	0.0	0.0	0.0	0.0
22	0.08232272847723411	0.0	0.0	0.0	0.0
23	0.08875266939971631	0.0	0.0	0.0	0.0
24	0.0976181940049569	0.0	0.0	0.0	0.0
25	0.10395071158012875	0.0	0.0	0.0	0.0
26	0.11174457936495566	0.0	0.0	0.0	0.0
27	0.11934360045516187	0.0	0.0	0.0	0.0
28	0.1333725624678503	0.0	0.0	0.0	0.0
29	0.14019219677957384	0.0	0.0	0.0	0.0
30	0.14730410113322837	0.0	0.0	0.0	0.0
31	0.15431858213957259	0.0	0.0	0.0	0.0
32	0.16182017988246847	0.0	0.0	0.0	0.0
33	0.16766558072108864	0.0	0.0	0.0	0.0
34	0.17701822206288093	0.0	0.0	0.0	0.0
35	0.19854878181846522	0.0	0.0	0.0	0.0
36	0.20731688307639548	0.0	0.0	0.0	0.0
37	0.21520817420853272	0.0	0.0	0.0	0.0
38	0.22173553847832525	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGCT	65	0.0	44.000004	29
CTATGCG	40	8.310963E-9	44.0	10
TCGTTAT	25	4.442506E-5	44.0	27
TACCCGA	30	2.5274512E-6	44.0	29
ACACGAT	50	2.7284841E-11	44.0	36
GGTAATC	45	4.802132E-10	44.0	9
GTATTCG	45	4.802132E-10	44.0	27
TTAATCG	20	7.8560883E-4	44.0	22
ATAGGTA	30	2.5274512E-6	44.0	34
CGAGTAC	25	4.442506E-5	44.0	42
TAGGTCG	75	0.0	44.0	19
GACGTAT	20	7.8560883E-4	44.0	25
CTATAAC	20	7.8560883E-4	44.0	33
CGCATAC	20	7.8560883E-4	44.0	17
TCTTATC	20	7.8560883E-4	44.0	13
TAGCGAA	25	4.442506E-5	44.0	41
ATTCGTT	25	4.442506E-5	44.0	33
TATATCG	20	7.8560883E-4	44.0	33
CGATTTC	30	2.5274512E-6	44.0	28
ATTCGAA	30	2.5274512E-6	44.0	10
>>END_MODULE