FastQCFastQC Report
Fri 17 Jun 2016
SRR1527170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099686
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGATCAACAGACT29130.26489379695658577No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT27830.25307224062141376No Hit
AACGCCGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA25860.23515803602119154No Hit
AACGCCGGGACCTTTGTATTATTGTGATAGCTTGTTGCTTATTATTTTATAC19570.17795989036870524No Hit
AACGCCGGGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGG18340.16677487937465785No Hit
AACGCCGGAGATGAATTTCTCATGTGGTCCATATCTCAACATTTGCTTTTTA17190.15631734877046718No Hit
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG16070.14613262331247281No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15720.1429498966068496No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC14210.12921870424830362No Hit
AACGCCGGGATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTTCT12850.11685153762073901No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC12540.11403255111004415No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC12400.11275946042779485No Hit
AACGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT12060.10966766877090368No Hit
AACGCCGGGACCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGC12020.10930392857597532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT351.0195072E-746.00000426
CGATTGG351.0195072E-746.00000423
ATCGTAA1050.046.00000443
CACTAGA351.0195072E-746.00000428
TACGAAA351.0195072E-746.00000420
TCGTAAT1050.046.00000444
AACGTCT301.86133E-646.013
AACGTAC206.311335E-446.044
GATACGA551.8189894E-1246.09
CCGATTA253.417058E-546.012
TAGACTA206.311335E-446.013
ACGCAAT301.86133E-646.041
ATTCGTT206.311335E-446.033
GTCTATA206.311335E-446.025
CACGATA253.417058E-546.037
CGCGATT206.311335E-446.017
TAACGGC206.311335E-446.029
CGCGAAA253.417058E-546.017
ATCGTAC253.417058E-546.032
AAACGCG301.86133E-646.022
Produced by FastQC (version 0.11.5)