##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1099686
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.12512844575633	33.0	31.0	34.0	31.0	34.0
2	32.79677562504206	34.0	31.0	34.0	31.0	34.0
3	32.470850770128926	34.0	31.0	34.0	31.0	34.0
4	36.07041919238765	37.0	35.0	37.0	35.0	37.0
5	36.21579341739369	37.0	37.0	37.0	35.0	37.0
6	36.37655385264521	37.0	37.0	37.0	35.0	37.0
7	36.469694076309054	37.0	37.0	37.0	35.0	37.0
8	36.58223438326941	37.0	37.0	37.0	35.0	37.0
9	38.65328466489525	39.0	39.0	39.0	38.0	39.0
10	38.03527461475367	39.0	38.0	39.0	37.0	39.0
11	37.73338843997286	39.0	38.0	39.0	35.0	39.0
12	37.3958620915425	39.0	37.0	39.0	35.0	39.0
13	37.319072898991166	39.0	37.0	39.0	35.0	39.0
14	38.216740051250994	40.0	38.0	40.0	35.0	40.0
15	38.06823766056856	40.0	38.0	40.0	34.0	40.0
16	32.96430890272314	40.0	35.0	40.0	3.0	40.0
17	34.47945686314093	40.0	35.0	40.0	16.0	40.0
18	36.53515548983983	40.0	35.0	40.0	30.0	40.0
19	37.51505975342052	40.0	37.0	40.0	33.0	40.0
20	37.82369421816773	40.0	37.0	40.0	33.0	40.0
21	37.98785198683988	40.0	38.0	40.0	34.0	40.0
22	38.02824533548667	40.0	38.0	40.0	34.0	40.0
23	37.95558550349827	40.0	38.0	40.0	34.0	40.0
24	37.86732576389988	40.0	37.0	40.0	34.0	40.0
25	37.78028819135644	40.0	37.0	40.0	33.0	40.0
26	37.63796756528682	40.0	37.0	40.0	33.0	40.0
27	37.4613426014335	40.0	36.0	40.0	33.0	40.0
28	37.28164948903596	39.0	36.0	40.0	33.0	40.0
29	37.09811255212852	39.0	35.0	40.0	33.0	40.0
30	36.987694669205574	39.0	35.0	40.0	32.0	40.0
31	36.90294320378726	39.0	35.0	40.0	32.0	40.0
32	36.76340428085835	39.0	35.0	40.0	32.0	40.0
33	36.39958860983953	38.0	35.0	40.0	31.0	40.0
34	36.3204978512048	38.0	35.0	40.0	31.0	40.0
35	36.15071393106759	38.0	35.0	40.0	31.0	40.0
36	36.04006598247136	38.0	35.0	40.0	31.0	40.0
37	35.685644811337056	37.0	35.0	40.0	30.0	40.0
38	35.654765996839096	37.0	34.0	40.0	30.0	40.0
39	35.28155764463674	37.0	34.0	40.0	29.0	40.0
40	35.23624652855452	36.0	34.0	40.0	29.0	40.0
41	35.13794846892659	36.0	34.0	40.0	29.0	40.0
42	35.025265393939726	36.0	34.0	39.0	29.0	40.0
43	34.80896910572655	35.0	34.0	39.0	29.0	40.0
44	35.021523416684396	36.0	34.0	39.0	29.0	40.0
45	35.076661883483105	35.0	34.0	39.0	30.0	40.0
46	34.995873367488535	35.0	34.0	39.0	30.0	40.0
47	34.67967947214023	35.0	34.0	39.0	29.0	40.0
48	34.61313775023052	35.0	34.0	39.0	29.0	40.0
49	34.54441085909978	35.0	34.0	39.0	29.0	40.0
50	34.019620146114434	35.0	34.0	38.0	27.0	40.0
51	34.039678599163764	35.0	34.0	37.0	27.0	40.0
52	33.709855358711486	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	2.0
15	12.0
16	28.0
17	56.0
18	139.0
19	299.0
20	605.0
21	960.0
22	1629.0
23	2414.0
24	3474.0
25	4859.0
26	6991.0
27	9088.0
28	11971.0
29	15426.0
30	20646.0
31	26470.0
32	35433.0
33	51610.0
34	80396.0
35	119605.0
36	176447.0
37	219996.0
38	232464.0
39	78663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.2020449473759	0.39493091664347824	0.09129878892702098	0.3117253470536135
8	99.17021768031965	0.5525213560961947	0.13021898978435661	0.14704197379979378
9	95.76015335286618	2.280741957249615	0.8175060881015127	1.1415986017826907
10	49.743926902770426	35.81240463186764	5.671709924469349	8.771958540892582
11	30.75532470177851	23.344300100210425	26.31987676482196	19.58049843318911
12	28.282982596850374	15.945733600318638	27.937156606522223	27.834127196308767
13	23.025299949258244	18.028691826575947	32.45344580180161	26.492562422364202
14	24.16053309762969	19.0608046296852	30.96765804056794	25.811004232117167
15	24.233463006712824	21.62708263995359	28.216418141178483	25.923036212155104
16	27.04662967428884	28.951809880274915	22.817604297954144	21.1839561474821
17	28.122664105935698	20.84204036424943	24.854549389553018	26.180746140261856
18	25.961956413012445	21.950811413439837	24.578015906358726	27.509216267188997
19	26.981792984542864	19.9306893058564	25.867202092233597	27.22031561736714
20	27.808574447615047	20.441198669438368	25.794726858394124	25.955500024552464
21	27.60360684777291	20.141749553963585	26.707714747664333	25.546928850599173
22	28.203960039502185	20.56905334795569	25.192464030641474	26.034522581900653
23	26.414085475308408	21.740387710673776	25.35996639040599	26.48556042361183
24	27.076820110467896	23.07413207042738	24.314577070181855	25.53447074892287
25	26.216392679364837	24.564193778951445	23.273461697248123	25.945951844435594
26	26.947237666024666	23.31338218364151	23.53353593662191	26.205844213711917
27	27.10228192411288	20.432832644955017	24.84754739080065	27.617338040131457
28	26.068714160223916	21.912527757923623	24.795805348072086	27.22295273378037
29	28.33845297657695	21.027456928614168	24.59911283766457	26.03497725714431
30	26.86521425206832	21.604257942721834	23.129056839861562	28.40147096534829
31	28.038912926053438	21.558972288453248	23.755508390576946	26.64660639491637
32	27.66526081081327	22.12431548642067	25.0536971462763	25.15672655648976
33	28.20077731279656	21.837688212817113	24.888650032827552	25.072884441558774
34	27.84904054430083	22.790323783334514	25.63504491282057	23.725590759544087
35	26.82056514314086	24.117338949481944	25.711885028999188	23.35021087837801
36	28.704375612674895	23.576548214672187	24.147711255758463	23.571364916894456
37	27.22849977175303	25.244297008418766	22.94973292376187	24.57747029606633
38	26.668612676709536	24.575378789945493	24.008762501295823	24.747246032049148
39	27.68181098968251	23.29064842145849	23.029301091402456	25.99823949745655
40	26.527845221272255	25.693970824398964	23.59609925014959	24.182084704179193
41	26.340519020884145	24.126159649208955	23.41713907424483	26.116182255662068
42	26.72917541916511	23.15597361428626	23.61646870106558	26.498382265483055
43	25.48081907017094	22.722031561736713	26.269044072580716	25.528105295511626
44	25.00713840132547	22.643009004388524	26.578496043416028	25.771356550869978
45	25.552657758669294	22.269175019050895	25.640955690988154	26.53721153129166
46	26.62141738641758	23.207442851868624	25.192645900738935	24.978493860974858
47	25.82200737301375	23.25409253186819	26.05098182572116	24.8729182693969
48	26.493108032656593	23.28164585163401	25.118533836022284	25.10671227968711
49	25.50137039118439	23.481612023795883	26.79883166649389	24.218185918525833
50	24.674952668307135	24.167353226284593	24.912202210449163	26.245491894959105
51	25.127081730603102	23.85853780079041	25.07015639009681	25.94422407850968
52	25.633408081943394	23.883544939191733	25.271122847794736	25.21192413107014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	25.0
12	50.0
13	301.5
14	1542.5
15	2532.0
16	3726.5
17	4921.0
18	4574.5
19	4228.0
20	4178.0
21	4128.0
22	3971.5
23	3815.0
24	4095.5
25	4376.0
26	4705.0
27	5034.0
28	5292.0
29	5550.0
30	6838.5
31	8127.0
32	8940.5
33	9754.0
34	12217.5
35	14681.0
36	13082.0
37	11483.0
38	13270.0
39	19394.5
40	23732.0
41	25513.5
42	27295.0
43	30422.0
44	33549.0
45	35627.0
46	37705.0
47	40463.0
48	43221.0
49	42623.0
50	42025.0
51	45772.5
52	49520.0
53	50827.5
54	52135.0
55	58371.0
56	64607.0
57	68281.5
58	71956.0
59	76664.5
60	81373.0
61	88617.0
62	95861.0
63	90729.5
64	82473.5
65	79349.0
66	70792.5
67	62236.0
68	55199.0
69	48162.0
70	41775.5
71	35389.0
72	29027.0
73	22665.0
74	21437.5
75	20210.0
76	18704.0
77	17198.0
78	11713.5
79	6229.0
80	4960.0
81	3691.0
82	2387.5
83	1084.0
84	759.0
85	434.0
86	273.5
87	113.0
88	79.5
89	26.5
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1099686.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.990974924428041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.63562046723067	8.469899537939792
2	10.119510413794314	2.4268559123859212
3	4.108771847744698	1.4780454058950765
4	2.242431400676489	1.0755575478104733
5	1.3642410281535176	0.8179289979732379
6	0.9492363546139018	0.6829361595318469
7	0.6275791957129477	0.5267700479220672
8	0.5102774289788294	0.4894979084309405
9	0.3961717285085699	0.42754367360822176
>10	4.734447366917852	13.886268774406735
>50	2.2566557580691873	19.700966954238318
>100	1.9722296168166997	41.76214467958943
>500	0.06876236381928272	5.573245616805556
>1k	0.014065028963035102	2.682338783462383
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACGCCGGGAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGATCAACAGACT	2913	0.26489379695658577	No Hit
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2783	0.25307224062141376	No Hit
AACGCCGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA	2586	0.23515803602119154	No Hit
AACGCCGGGACCTTTGTATTATTGTGATAGCTTGTTGCTTATTATTTTATAC	1957	0.17795989036870524	No Hit
AACGCCGGGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGG	1834	0.16677487937465785	No Hit
AACGCCGGAGATGAATTTCTCATGTGGTCCATATCTCAACATTTGCTTTTTA	1719	0.15631734877046718	No Hit
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1607	0.14613262331247281	No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1572	0.1429498966068496	No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1421	0.12921870424830362	No Hit
AACGCCGGGATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTTCT	1285	0.11685153762073901	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1254	0.11403255111004415	No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1240	0.11275946042779485	No Hit
AACGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1206	0.10966766877090368	No Hit
AACGCCGGGACCAGACAATTGAAGAGAACATTAAGATCTTTGAGGAAGACGC	1202	0.10930392857597532	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.456102923925557E-4	0.0	0.0	0.0	0.0
8	0.0010002855360530189	0.0	0.0	0.0	0.0
9	0.0012730906822492967	0.0	0.0	0.0	0.0
10	0.0037283369980157972	0.0	0.0	0.0	0.0
11	0.006547323508710668	0.0	0.0	0.0	0.0
12	0.008093219337156243	0.0	0.0	0.0	0.0
13	0.012276231578832503	0.0	0.0	0.0	0.0
14	0.023461242572879897	0.0	0.0	0.0	0.0
15	0.031736332007500326	0.0	0.0	0.0	0.0
16	0.041284512124370044	0.0	0.0	0.0	0.0
17	0.048104640779276994	0.0	0.0	0.0	0.0
18	0.05201484787475698	0.0	0.0	0.0	0.0
19	0.062017703235287165	0.0	0.0	0.0	0.0
20	0.06601884537949924	0.0	0.0	0.0	0.0
21	0.07229336374201363	0.0	0.0	0.0	0.0
22	0.07820414190959965	0.0	0.0	0.0	0.0
23	0.08484240046704242	0.0	0.0	0.0	0.0
24	0.09420871048644795	0.0	0.0	0.0	0.0
25	0.10021042370276606	0.0	0.0	0.0	0.0
26	0.10775803274752975	0.0	0.0	0.0	0.0
27	0.11339600576891949	0.0	0.0	0.0	0.0
28	0.1269453280300013	0.0	0.0	0.0	0.0
29	0.13240143095392684	0.0	0.0	0.0	0.0
30	0.14085839048601145	0.0	0.0	0.0	0.0
31	0.1467691686535975	0.0	0.0	0.0	0.0
32	0.15313462206484396	0.0	0.0	0.0	0.0
33	0.15950007547609044	0.0	0.0	0.0	0.0
34	0.1673204896670504	0.0	0.0	0.0	0.0
35	0.18950864155768102	0.0	0.0	0.0	0.0
36	0.19760186089483725	0.0	0.0	0.0	0.0
37	0.20396731430608372	0.0	0.0	0.0	0.0
38	0.21269707898436463	0.0	0.0	0.0	0.0
39	0.2216087137601097	0.0	0.0	0.0	0.0
40	0.22970193309726594	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	35	1.0195072E-7	46.000004	26
CGATTGG	35	1.0195072E-7	46.000004	23
ATCGTAA	105	0.0	46.000004	43
CACTAGA	35	1.0195072E-7	46.000004	28
TACGAAA	35	1.0195072E-7	46.000004	20
TCGTAAT	105	0.0	46.000004	44
AACGTCT	30	1.86133E-6	46.0	13
AACGTAC	20	6.311335E-4	46.0	44
GATACGA	55	1.8189894E-12	46.0	9
CCGATTA	25	3.417058E-5	46.0	12
TAGACTA	20	6.311335E-4	46.0	13
ACGCAAT	30	1.86133E-6	46.0	41
ATTCGTT	20	6.311335E-4	46.0	33
GTCTATA	20	6.311335E-4	46.0	25
CACGATA	25	3.417058E-5	46.0	37
CGCGATT	20	6.311335E-4	46.0	17
TAACGGC	20	6.311335E-4	46.0	29
CGCGAAA	25	3.417058E-5	46.0	17
ATCGTAC	25	3.417058E-5	46.0	32
AAACGCG	30	1.86133E-6	46.0	22
>>END_MODULE