FastQCFastQC Report
Fri 17 Jun 2016
SRR1527169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1069200
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT29740.2781518892630004No Hit
AACGCCGGGAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGATCAACAGACT28660.2680508791619903No Hit
AACGCCGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA25390.237467265245043No Hit
AACGCCGGGACCTTTGTATTATTGTGATAGCTTGTTGCTTATTATTTTATAC18740.17527123082678636No Hit
AACGCCGGAGATGAATTTCTCATGTGGTCCATATCTCAACATTTGCTTTTTA16410.15347923681257014No Hit
AACGCCGGGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGG16040.15001870557426114No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15190.14206883651328098No Hit
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG14620.13673774784885895No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC13900.13000374111485224No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC12320.11522633744855967No Hit
AACGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT12120.11335578002244669No Hit
AACGCCGGGATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTTCT11610.10858585858585859No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC11450.10708941264496821No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT206.3112547E-446.00000429
GATACGG206.3112547E-446.00000442
TTAGACC206.3112547E-446.00000446
CATTACG206.3112547E-446.00000418
CCGTAAA405.6115823E-946.00000429
CTTATCG206.3112547E-446.00000423
TACGATT206.3112547E-446.00000412
CGTAAAT206.3112547E-446.00000420
ATACGAT206.3112547E-446.00000410
TATACGC206.3112547E-446.00000421
CGGAATT253.416994E-546.06
GTCGACG253.416994E-546.015
GATCACG253.416994E-546.09
AATGCGA253.416994E-546.015
CGTAAGT253.416994E-546.025
ATACGCA253.416994E-546.022
TTCGATA301.8612809E-646.027
TACACTA253.416994E-546.044
TACGTAC253.416994E-546.037
GATACGA351.019489E-745.9999969
Produced by FastQC (version 0.11.5)