##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1069200
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07593621399177	33.0	31.0	34.0	31.0	34.0
2	32.769946689113354	34.0	31.0	34.0	31.0	34.0
3	32.43863355780022	34.0	31.0	34.0	31.0	34.0
4	36.07180041152263	37.0	35.0	37.0	35.0	37.0
5	36.229956041900486	37.0	37.0	37.0	35.0	37.0
6	36.38197250280584	37.0	37.0	37.0	35.0	37.0
7	36.47028245417134	37.0	37.0	37.0	35.0	37.0
8	36.60473344556678	37.0	37.0	37.0	35.0	37.0
9	38.593728020950245	39.0	39.0	39.0	38.0	39.0
10	38.03416573138795	39.0	38.0	39.0	37.0	39.0
11	37.66702861952862	39.0	38.0	39.0	35.0	39.0
12	37.35622427983539	39.0	37.0	39.0	35.0	39.0
13	37.228458660680886	39.0	37.0	39.0	34.0	39.0
14	38.14250374111485	40.0	38.0	40.0	34.0	40.0
15	38.182332585110366	40.0	38.0	40.0	34.0	40.0
16	38.248612981668536	40.0	38.0	40.0	35.0	40.0
17	38.21150673400673	40.0	38.0	40.0	35.0	40.0
18	38.20890198279087	40.0	38.0	40.0	34.0	40.0
19	38.13416760194538	40.0	38.0	40.0	34.0	40.0
20	38.08288533482978	40.0	38.0	40.0	34.0	40.0
21	33.66182285821175	40.0	34.0	40.0	10.0	40.0
22	34.320430228208004	39.0	34.0	40.0	15.0	40.0
23	36.37329218106996	39.0	34.0	40.0	30.0	40.0
24	37.16202207257763	39.0	36.0	40.0	32.0	40.0
25	37.46828563411897	39.0	37.0	40.0	33.0	40.0
26	37.511208380097266	40.0	37.0	40.0	33.0	40.0
27	37.41572951739619	40.0	36.0	40.0	33.0	40.0
28	37.256730265619154	39.0	36.0	40.0	33.0	40.0
29	37.24375140291807	39.0	35.0	40.0	33.0	40.0
30	37.05391507669285	39.0	35.0	40.0	33.0	40.0
31	36.86652731013842	39.0	35.0	40.0	32.0	40.0
32	36.753555929667044	39.0	35.0	40.0	32.0	40.0
33	36.40757669285447	38.0	35.0	40.0	31.0	40.0
34	36.3216835016835	38.0	35.0	40.0	31.0	40.0
35	36.154588477366254	38.0	35.0	40.0	31.0	40.0
36	35.97536756453423	38.0	35.0	40.0	31.0	40.0
37	35.83246539468762	37.0	35.0	40.0	30.0	40.0
38	35.73771043771044	37.0	35.0	40.0	30.0	40.0
39	35.58521137298915	37.0	34.0	40.0	30.0	40.0
40	30.758178077066965	35.0	30.0	39.0	8.0	40.0
41	31.504740927796483	35.0	30.0	39.0	12.0	40.0
42	33.22057706696596	35.0	30.0	39.0	23.0	40.0
43	33.995381593714924	35.0	32.0	39.0	26.0	40.0
44	34.69854283576506	35.0	33.0	39.0	28.0	40.0
45	34.94766647961092	35.0	34.0	39.0	30.0	40.0
46	34.95858305274972	35.0	34.0	39.0	30.0	40.0
47	34.88953984287318	35.0	34.0	39.0	30.0	40.0
48	34.736743359521135	35.0	34.0	39.0	29.0	40.0
49	34.61217171717172	35.0	34.0	39.0	29.0	40.0
50	34.45087448559671	35.0	34.0	38.0	29.0	40.0
51	34.240038346427234	35.0	34.0	38.0	29.0	40.0
52	33.851286943509166	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	7.0
16	25.0
17	65.0
18	150.0
19	302.0
20	635.0
21	1028.0
22	1642.0
23	2509.0
24	3580.0
25	5318.0
26	7144.0
27	9602.0
28	12570.0
29	16812.0
30	21951.0
31	29630.0
32	41354.0
33	56729.0
34	90894.0
35	123840.0
36	161729.0
37	174035.0
38	226739.0
39	80908.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	99.22465394687617	0.3889824167601945	0.09436962214739993	0.29199401421623644
8	99.19500561167229	0.5524691358024691	0.11307519640852974	0.1394500561167228
9	95.72961092405536	2.283576505798728	0.8636363636363636	1.1231762065095399
10	49.77169846614291	35.78208005985784	5.70894126449682	8.737280209502432
11	30.949869060980173	23.33539094650206	26.226524504302283	19.488215488215488
12	28.3228582117471	15.955013093901982	27.84175084175084	27.880377852600073
13	23.05396558174336	18.059670781893004	32.39450056116723	26.49186307519641
14	24.160306771417883	19.039001122334458	31.00645342312009	25.794238683127574
15	24.206041900486344	21.555929667040775	28.25729517396184	25.980733258511034
16	28.202300785634122	21.275626636737748	25.93677515899738	24.585297418630752
17	28.16965955854845	20.92199775533109	24.745043022820802	26.16329966329966
18	25.93593340815563	22.019453797231574	24.586606808829032	27.458005985783764
19	26.976243920688365	20.011316872427983	25.838570894126452	27.1738683127572
20	27.708005985783764	20.44481855592967	25.823045267489714	26.024130190796857
21	32.44145155256266	19.486438458660682	25.17732884399551	22.894781144781145
22	28.374392068836514	20.52702955480733	25.147586980920316	25.950991395435842
23	26.403759820426487	21.759072203516645	25.31537598204265	26.521791994014215
24	27.19799850355406	23.065843621399175	24.228020950243174	25.508136924803594
25	26.264683875794987	24.596427235316124	23.258230452674898	25.880658436213995
26	26.988402543958102	23.39188178077067	23.366629255518145	26.253086419753085
27	27.098110736999626	20.412083800972688	24.838664421997755	27.65114104002993
28	26.083333333333332	21.939955106621774	24.744294799850355	27.23241676019454
29	28.25542461653573	21.108305274971944	24.634867190422746	26.001402918069584
30	26.84717545828657	21.620463898241677	23.091657313879534	28.44070332959222
31	28.07846988402544	21.614945753834643	23.706790123456788	26.599794238683128
32	27.722876917321358	22.20164609053498	24.88627010849233	25.189206883651327
33	28.18331462775907	21.828282828282827	24.939768050879163	25.048634493078936
34	27.8426861204639	22.828376356154134	25.528526000748226	23.800411522633745
35	26.8410961466517	24.12037037037037	25.697904975682757	23.340628507295175
36	28.727366255144034	23.57865693976805	24.151234567901234	23.54274223718668
37	27.151515151515156	25.113636363636367	23.014216236438457	24.720632248410027
38	26.59988776655443	24.52600074822297	24.042368125701458	24.831743359521138
39	27.48971193415638	23.25710811821923	23.062850729517397	26.190329218106996
40	27.151234567901234	29.759259259259256	21.590347923681257	21.49915824915825
41	26.421530115974562	24.152731013842125	23.35578002244669	26.069958847736626
42	26.742330714552935	23.24055368499813	23.543677515899738	26.473438084549194
43	25.490927796483355	22.719042274597832	26.26094276094276	25.529087167976055
44	24.96081182192293	22.63711185933408	26.62878787878788	25.773288439955106
45	25.684811073699965	22.211279461279464	25.55780022446689	26.546109240553683
46	26.6805087916199	23.315001870557424	25.118312757201643	24.886176580621026
47	25.578750467639356	23.240460157126826	26.113355780022445	25.067433595211376
48	26.423868312757204	23.30677141788253	25.14964459408904	25.11971567527123
49	25.47465394687617	23.53329592218481	26.806864945753833	24.185185185185183
50	24.585671530115974	24.15628507295174	25.05564908342686	26.202394313505422
51	25.19519266741489	23.816217732884397	25.113449307893752	25.875140291806957
52	25.776842499064724	23.92433595211373	25.188271604938272	25.110549943883274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	1.5
10	3.0
11	30.0
12	57.0
13	305.5
14	1476.5
15	2399.0
16	3466.0
17	4533.0
18	4276.5
19	4020.0
20	4040.5
21	4061.0
22	3988.5
23	3916.0
24	4050.5
25	4185.0
26	4580.5
27	4976.0
28	5198.0
29	5420.0
30	6603.0
31	7786.0
32	8378.5
33	8971.0
34	11630.0
35	14289.0
36	12971.5
37	11654.0
38	13031.0
39	18244.0
40	22080.0
41	23922.0
42	25764.0
43	29049.0
44	32334.0
45	33951.0
46	35568.0
47	38670.0
48	41772.0
49	41434.5
50	41097.0
51	44786.5
52	48476.0
53	49618.0
54	50760.0
55	57005.5
56	63251.0
57	66303.0
58	69355.0
59	73951.0
60	78547.0
61	85812.5
62	93078.0
63	88187.0
64	80864.5
65	78433.0
66	69974.0
67	61515.0
68	54359.5
69	47204.0
70	41224.0
71	35244.0
72	28742.0
73	22240.0
74	20722.5
75	19205.0
76	18052.5
77	16900.0
78	11637.5
79	6375.0
80	5062.5
81	3750.0
82	2449.0
83	1148.0
84	795.0
85	442.0
86	260.0
87	78.0
88	64.0
89	26.5
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1069200.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.257175729909399
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.42062819866723	8.141293119220396
2	11.021334760275888	2.701808738697208
3	5.489590295456445	2.0186061880984467
4	3.155960764921196	1.5473266276935354
5	1.961719881306022	1.202257265901246
6	1.3400468435085564	0.9855113788316865
7	0.9379724621974662	0.8047825309279101
8	0.684839700301047	0.6715360442726735
9	0.48527806853165517	0.5353324707477163
>10	4.282842177614079	12.798697170215261
>50	2.2475732479237256	19.980974744516082
>100	1.8908029205721968	40.54677664026804
>500	0.0694385200885526	5.6473288946540645
>1k	0.011972158635957344	2.4177681859557314
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AACGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2974	0.2781518892630004	No Hit
AACGCCGGGAAGATAGTAATAGAAAAGGGGGCTGCAAAAAGATCAACAGACT	2866	0.2680508791619903	No Hit
AACGCCGGGATGTTACTTTATGCTGCTGGCTCTTGTACCTTGGGAGATTGTA	2539	0.237467265245043	No Hit
AACGCCGGGACCTTTGTATTATTGTGATAGCTTGTTGCTTATTATTTTATAC	1874	0.17527123082678636	No Hit
AACGCCGGAGATGAATTTCTCATGTGGTCCATATCTCAACATTTGCTTTTTA	1641	0.15347923681257014	No Hit
AACGCCGGGCTCGGGATATTTTTCAATGTCCATTCTGCTGTGTTAATTGAGG	1604	0.15001870557426114	No Hit
AACGCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1519	0.14206883651328098	No Hit
AACGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	1462	0.13673774784885895	No Hit
AACGCCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	1390	0.13000374111485224	No Hit
AACGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1232	0.11522633744855967	No Hit
AACGCCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1212	0.11335578002244669	No Hit
AACGCCGGGATCGGCATTGGCATTGCACTCTCAGGCCTGCCCTTCTACTTCT	1161	0.10858585858585859	No Hit
AACGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1145	0.10708941264496821	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.611672278338945E-4	0.0	0.0	0.0	0.0
8	9.352787130564908E-4	0.0	0.0	0.0	0.0
9	9.352787130564908E-4	0.0	0.0	0.0	0.0
10	0.0032734754956977178	0.0	0.0	0.0	0.0
11	0.0051440329218107	0.0	0.0	0.0	0.0
12	0.008511036288814066	0.0	0.0	0.0	0.0
13	0.013374485596707819	0.0	0.0	0.0	0.0
14	0.023381967826412272	0.0	0.0	0.0	0.0
15	0.03170594837261504	0.0	0.0	0.0	0.0
16	0.0402169846614291	0.0	0.0	0.0	0.0
17	0.04835390946502058	0.0	0.0	0.0	0.0
18	0.05396558174335952	0.0	0.0	0.0	0.0
19	0.06219603441825664	0.0	0.0	0.0	0.0
20	0.06640478862701085	0.0	0.0	0.0	0.0
21	0.07248410026187804	0.0	0.0	0.0	0.0
22	0.07865693976805088	0.0	0.0	0.0	0.0
23	0.08473625140291807	0.0	0.0	0.0	0.0
24	0.09549195660306771	0.0	0.0	0.0	0.0
25	0.10447063224841002	0.0	0.0	0.0	0.0
26	0.11064347175458286	0.0	0.0	0.0	0.0
27	0.11765806210250655	0.0	0.0	0.0	0.0
28	0.13112607557052003	0.0	0.0	0.0	0.0
29	0.1358959970071081	0.0	0.0	0.0	0.0
30	0.14524878413767303	0.0	0.0	0.0	0.0
31	0.15057987280209503	0.0	0.0	0.0	0.0
32	0.15684624017957352	0.0	0.0	0.0	0.0
33	0.16180321735877293	0.0	0.0	0.0	0.0
34	0.16909839132061355	0.0	0.0	0.0	0.0
35	0.18855218855218855	0.0	0.0	0.0	0.0
36	0.195099139543584	0.0	0.0	0.0	0.0
37	0.20117845117845118	0.0	0.0	0.0	0.0
38	0.20997007108118218	0.0	0.0	0.0	0.0
39	0.21782641227085672	0.0	0.0	0.0	0.0
40	0.2277403666292555	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	20	6.3112547E-4	46.000004	29
GATACGG	20	6.3112547E-4	46.000004	42
TTAGACC	20	6.3112547E-4	46.000004	46
CATTACG	20	6.3112547E-4	46.000004	18
CCGTAAA	40	5.6115823E-9	46.000004	29
CTTATCG	20	6.3112547E-4	46.000004	23
TACGATT	20	6.3112547E-4	46.000004	12
CGTAAAT	20	6.3112547E-4	46.000004	20
ATACGAT	20	6.3112547E-4	46.000004	10
TATACGC	20	6.3112547E-4	46.000004	21
CGGAATT	25	3.416994E-5	46.0	6
GTCGACG	25	3.416994E-5	46.0	15
GATCACG	25	3.416994E-5	46.0	9
AATGCGA	25	3.416994E-5	46.0	15
CGTAAGT	25	3.416994E-5	46.0	25
ATACGCA	25	3.416994E-5	46.0	22
TTCGATA	30	1.8612809E-6	46.0	27
TACACTA	25	3.416994E-5	46.0	44
TACGTAC	25	3.416994E-5	46.0	37
GATACGA	35	1.019489E-7	45.999996	9
>>END_MODULE