FastQCFastQC Report
Fri 17 Jun 2016
SRR1527168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764400
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC61560.34890047608252095No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC54580.3093402856495126No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51960.29449104511448654No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG50900.28848333711176605No Hit
AAAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA47550.2694967127635457No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC44200.25051008841532535No Hit
AAAACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT41880.23736114259805033No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT41690.236284289276808No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT38140.2161641351167536No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA38070.21576739968261166No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC32010.1814214463840399No Hit
AAAACGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA30740.17422353207889368No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG29690.1682725005667649No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC29320.1661754704148719No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG28250.16011108592155973No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC27230.15433008388120606No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT25750.1459419632736341No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT22030.12485830877352075No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21670.12281795511221946No Hit
AAAACGGGCCTGGTGGTGGATTATACAAGAGACTAGAATTGCACCTGGGT21570.12225119020630243No Hit
AAAACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC21250.12043754250736795No Hit
AAAACGGGGGCTGCATCCACACTGACTGAGAGGCCTTGCTATAACCGGAG20960.11879392428020857No Hit
AAAACGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC20630.11692360009068238No Hit
AAAACGGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGA20560.11652686465654047No Hit
AAAACGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC19560.1108592155973702No Hit
AAAACGGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC19280.10927227386080254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACTT950.044.00000437
CAAACCG408.3164196E-944.021
ACACGAT254.4438122E-544.010
CGTATAA254.4438122E-544.042
CCGATTT254.4438122E-544.043
TTTACGA207.85763E-444.043
ACGCATG207.85763E-444.020
CACGATT254.4438122E-544.022
TAACGTA207.85763E-444.034
CGATTCA351.4466787E-744.036
TCGATTG302.5284899E-644.028
CGACAGT207.85763E-444.022
ATGTACG254.4438122E-544.042
CCGTACT207.85763E-444.043
CTACGTA207.85763E-444.011
CGTAGAT254.4438122E-544.031
TACGAAT254.4438122E-544.016
CGAATGT454.802132E-1044.024
CGTACTC254.4438122E-544.015
CATACCG207.85763E-444.017
Produced by FastQC (version 0.11.5)