##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1764400
Sequences flagged as poor quality	0
Sequence length	50
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.383093402856495	33.0	31.0	34.0	31.0	34.0
2	32.99204715484017	34.0	31.0	34.0	31.0	34.0
3	33.24633699841306	34.0	34.0	34.0	31.0	34.0
4	36.62740308320109	37.0	37.0	37.0	35.0	37.0
5	36.292141237814555	37.0	37.0	37.0	35.0	37.0
6	36.22983280435275	37.0	37.0	37.0	35.0	37.0
7	36.6000957832691	37.0	37.0	37.0	35.0	37.0
8	36.66602017683065	37.0	37.0	37.0	35.0	37.0
9	38.650846180004535	39.0	39.0	39.0	39.0	39.0
10	38.144609498979825	39.0	39.0	39.0	37.0	39.0
11	36.97663738381319	39.0	35.0	39.0	34.0	39.0
12	36.70570618907277	39.0	35.0	39.0	33.0	39.0
13	36.673796758104736	39.0	35.0	39.0	33.0	39.0
14	37.48198367717071	40.0	35.0	40.0	33.0	40.0
15	37.546670822942644	40.0	35.0	40.0	33.0	40.0
16	37.4666096123328	40.0	35.0	40.0	33.0	40.0
17	37.49948877805486	40.0	35.0	40.0	33.0	40.0
18	37.493653366583544	40.0	35.0	40.0	33.0	40.0
19	37.433256064384494	40.0	35.0	40.0	33.0	40.0
20	37.14639084107912	40.0	35.0	40.0	33.0	40.0
21	37.282536272953976	40.0	35.0	40.0	33.0	40.0
22	37.11193210156427	40.0	35.0	40.0	33.0	40.0
23	37.12121571072319	39.0	35.0	40.0	33.0	40.0
24	37.144532985717525	39.0	35.0	40.0	33.0	40.0
25	37.08629789163455	39.0	35.0	40.0	33.0	40.0
26	36.93523520743596	39.0	35.0	40.0	33.0	40.0
27	36.766042280661985	39.0	35.0	40.0	32.0	40.0
28	36.54789390160961	38.0	35.0	40.0	32.0	40.0
29	36.71811947404217	39.0	35.0	40.0	32.0	40.0
30	36.736613579687145	39.0	35.0	40.0	32.0	40.0
31	36.66852527771481	39.0	35.0	40.0	32.0	40.0
32	35.95475572432555	38.0	35.0	40.0	31.0	40.0
33	36.05364769893448	38.0	35.0	40.0	31.0	40.0
34	36.02591872591249	38.0	35.0	40.0	31.0	40.0
35	35.96130412604852	37.0	35.0	40.0	31.0	40.0
36	35.744079007027885	37.0	35.0	40.0	31.0	40.0
37	35.62518306506461	37.0	35.0	40.0	30.0	40.0
38	35.388472001813646	36.0	35.0	40.0	30.0	40.0
39	35.319853207889366	36.0	35.0	40.0	30.0	40.0
40	35.14830197234187	35.0	34.0	40.0	30.0	40.0
41	34.96997959646339	35.0	34.0	40.0	29.0	40.0
42	34.85089435502154	35.0	34.0	39.0	29.0	40.0
43	34.74688789390161	35.0	34.0	39.0	29.0	40.0
44	34.61262072092496	35.0	34.0	39.0	29.0	40.0
45	34.49627012015416	35.0	34.0	39.0	28.0	40.0
46	34.22669972795285	35.0	34.0	39.0	27.0	40.0
47	34.13627522103831	35.0	34.0	38.0	27.0	40.0
48	34.00719054636137	35.0	34.0	38.0	27.0	40.0
49	33.70091305826343	35.0	33.0	37.0	26.0	40.0
50	33.351267853094534	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	6.0
14	11.0
15	31.0
16	92.0
17	240.0
18	456.0
19	841.0
20	1411.0
21	2206.0
22	3398.0
23	4915.0
24	6921.0
25	9362.0
26	12072.0
27	15477.0
28	20108.0
29	26447.0
30	33641.0
31	42149.0
32	54970.0
33	84158.0
34	180985.0
35	189424.0
36	202650.0
37	303583.0
38	395979.0
39	172866.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.56126728632964	0.33790523690773067	0.06733167082294264	0.03349580593969621
8	99.12644525051009	0.6624348220358196	0.1260485150759465	0.08507141237814556
9	94.05837678530945	2.802822489231467	1.6754704148719113	1.4633303105871684
10	54.91980276581274	29.788483337111764	6.137270460213103	9.154443436862389
11	34.76133529811834	22.472851961006572	21.032759011562003	21.73305372931308
12	28.23345046474722	16.76059850374065	28.606665155293587	26.399285876218542
13	24.206585808206757	17.644921786442982	30.22897302199048	27.91951938335978
14	24.23367717070959	17.886987077760143	31.797834958059397	26.08150079347087
15	25.541373838131943	18.888120607571977	27.53791657220585	28.03258898209023
16	30.088415325323055	19.526467921106324	24.500226705962366	25.88489004760825
17	29.938902743142144	19.90251643618227	23.200011335298118	26.958569485377463
18	29.884493312174108	19.667649059170255	22.664248469734755	27.78360915892088
19	28.643051462253453	19.864259805032873	22.618567218317843	28.874121514395828
20	28.970358195420538	21.52165041940603	23.84487644525051	25.66311493992292
21	30.21066651552936	19.058603491271818	25.05832010881886	25.67240988437996
22	31.022160507821354	18.650929494445702	24.74937655860349	25.577533439129454
23	29.680854681478124	20.397925640444345	23.418159147585584	26.50306053049195
24	29.727386080253908	20.783552482430288	22.224155520290186	27.264905917025615
25	28.91215143958286	20.743425527091365	22.082747676263885	28.26167535706189
26	28.48945817274994	20.74268873271367	21.698197687599183	29.0696554069372
27	29.445930627975514	18.83660167762412	22.91645885286783	28.801008841532532
28	30.033892541373834	20.06795511221945	21.72766946270687	28.170482883699844
29	28.97942643391521	19.855701654953524	22.996429381092724	28.16844253003854
30	28.65977102697801	20.51167535706189	23.48101337565178	27.34754024030832
31	30.690206302425754	20.917195647245524	21.329403763318975	27.06319428700975
32	32.465824076173206	20.1564271140331	21.658977556109726	25.71877125368397
33	31.809793697574246	20.84912718204489	22.217921106325097	25.123158014055768
34	29.123101337565178	22.087791883926545	24.32889367490365	24.460213103604627
35	29.835638177284064	23.313420992972116	23.781398775787803	23.06954205395602
36	30.783099070505553	21.862559510315123	22.15790070278848	25.19644071639084
37	31.17479029698481	22.39118113806393	21.1687259124915	25.26530265245976
38	30.99393561550669	22.482486964407165	21.89543187485831	24.628145545227838
39	31.585751530265245	20.25833144411698	22.434822035819543	25.72109498979823
40	28.60751530265246	22.120664248469733	22.41334164588529	26.85847880299252
41	28.387270460213106	21.833654500113354	22.416685558830196	27.362389480843348
42	26.638347313534343	20.519780095216504	24.718147812287462	28.123724778961684
43	27.893788256631147	19.531115393334844	25.334561323962816	27.240535026071182
44	26.304466107458623	20.561153933348447	25.446950804806168	27.687429154386763
45	27.54959192926774	19.206245749263207	24.2254024030832	29.018759918385857
46	28.908524144184994	20.608705508954888	23.234810700521425	27.2479596463387
47	27.50640444343686	20.199331217411018	24.977556109725686	27.316708229426435
48	28.964747222851962	20.582804352754476	24.668669236000905	25.783779188392653
49	27.673940149625935	21.58524144184992	24.107345273180687	26.63347313534346
50	25.667365676717296	22.134776694627067	24.205282248923147	27.992575379732486
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	21.5
6	41.0
7	455.5
8	870.0
9	2120.5
10	3371.0
11	2610.5
12	1850.0
13	1878.5
14	1907.0
15	2061.0
16	2215.0
17	2628.0
18	3041.0
19	3409.0
20	3777.0
21	3900.5
22	4024.0
23	4617.5
24	5211.0
25	5948.0
26	6685.0
27	7793.0
28	8901.0
29	10874.0
30	12847.0
31	15811.5
32	18776.0
33	19981.0
34	21186.0
35	22022.0
36	22858.0
37	24385.5
38	25913.0
39	28644.0
40	31375.0
41	33754.5
42	36134.0
43	42252.5
44	48371.0
45	48983.5
46	49596.0
47	55466.0
48	61336.0
49	70981.0
50	80626.0
51	89900.5
52	99175.0
53	106629.5
54	114084.0
55	144250.0
56	174416.0
57	177121.0
58	179826.0
59	175268.5
60	170711.0
61	162488.5
62	154266.0
63	142730.5
64	131195.0
65	110637.5
66	90080.0
67	77584.0
68	65088.0
69	58717.5
70	52347.0
71	42551.5
72	32756.0
73	33011.0
74	33266.0
75	21868.5
76	10471.0
77	7085.5
78	3700.0
79	2433.0
80	1166.0
81	904.0
82	642.0
83	427.0
84	212.0
85	138.5
86	65.0
87	42.5
88	20.0
89	10.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1764400.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.912408872189748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.25178330849019	5.4003601579286995
2	10.64402174494544	1.6843970418097383
3	4.697756337387881	1.1151170677000046
4	2.472397251175072	0.7825047178310075
5	1.5004143862845023	0.5935946050999316
6	0.8651950706248513	0.41074662917921456
7	0.615120169680757	0.34069576016314196
8	0.41288776004357985	0.26135494206298987
9	0.2928534728278547	0.2085458774989227
>10	3.808290420376631	8.063549087404434
>50	3.0013214712846326	17.435209003085685
>100	3.224855841042673	45.83562157721895
>500	0.14529734034272673	7.8550117888517175
>1k	0.06482496722983193	8.72214415289116
>5k	0.0029804582634405485	1.291147591274394
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	6156	0.34890047608252095	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5458	0.3093402856495126	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5196	0.29449104511448654	No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	5090	0.28848333711176605	No Hit
AAAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	4755	0.2694967127635457	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4420	0.25051008841532535	No Hit
AAAACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	4188	0.23736114259805033	No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	4169	0.236284289276808	No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3814	0.2161641351167536	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3807	0.21576739968261166	No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3201	0.1814214463840399	No Hit
AAAACGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA	3074	0.17422353207889368	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2969	0.1682725005667649	No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2932	0.1661754704148719	No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2825	0.16011108592155973	No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	2723	0.15433008388120606	No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	2575	0.1459419632736341	No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	2203	0.12485830877352075	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2167	0.12281795511221946	No Hit
AAAACGGGCCTGGTGGTGGATTATACAAGAGACTAGAATTGCACCTGGGT	2157	0.12225119020630243	No Hit
AAAACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC	2125	0.12043754250736795	No Hit
AAAACGGGGGCTGCATCCACACTGACTGAGAGGCCTTGCTATAACCGGAG	2096	0.11879392428020857	No Hit
AAAACGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2063	0.11692360009068238	No Hit
AAAACGGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGA	2056	0.11652686465654047	No Hit
AAAACGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTC	1956	0.1108592155973702	No Hit
AAAACGGGGGACTGTCGAGTCGCGTCCACCCGCGAGCACAGCTTCTTTGC	1928	0.10927227386080254	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.667649059170256E-4	0.0	0.0	0.0	0.0
8	9.06823849467241E-4	0.0	0.0	0.0	0.0
9	0.002720471548401723	0.0	0.0	0.0	0.0
10	0.011335298118340512	0.0	0.0	0.0	0.0
11	0.019780095216504194	0.0	0.0	0.0	0.0
12	0.026921333031058717	0.0	0.0	0.0	0.0
13	0.03428927680798005	0.0	0.0	0.0	0.0
14	0.046361369304012696	0.0	0.0	0.0	0.0
15	0.054749489911584674	0.0	0.0	0.0	0.0
16	0.06687825889820902	0.0	0.0	0.0	0.0
17	0.0753797324869644	0.0	0.0	0.0	0.0
18	0.08240761732033552	0.0	0.0	0.0	0.0
19	0.09130582634323282	0.0	0.0	0.0	0.0
20	0.09629335751530266	0.0	0.0	0.0	0.0
21	0.10150759464973928	0.0	0.0	0.0	0.0
22	0.10734527318068465	0.0	0.0	0.0	0.0
23	0.1148832464293811	0.0	0.0	0.0	0.0
24	0.12310133756517797	0.0	0.0	0.0	0.0
25	0.13075266379505782	0.0	0.0	0.0	0.0
26	0.1372137837225119	0.0	0.0	0.0	0.0
27	0.1462253457265926	0.0	0.0	0.0	0.0
28	0.15563364316481523	0.0	0.0	0.0	0.0
29	0.16266152799818634	0.0	0.0	0.0	0.0
30	0.1701995012468828	0.0	0.0	0.0	0.0
31	0.17807753343912944	0.0	0.0	0.0	0.0
32	0.18419859442303332	0.0	0.0	0.0	0.0
33	0.1902629789163455	0.0	0.0	0.0	0.0
34	0.19649739288143278	0.0	0.0	0.0	0.0
35	0.21191339832237588	0.0	0.0	0.0	0.0
36	0.22483563817728405	0.0	0.0	0.0	0.0
37	0.23118340512355476	0.0	0.0	0.0	0.0
38	0.23832464293810926	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACTT	95	0.0	44.000004	37
CAAACCG	40	8.3164196E-9	44.0	21
ACACGAT	25	4.4438122E-5	44.0	10
CGTATAA	25	4.4438122E-5	44.0	42
CCGATTT	25	4.4438122E-5	44.0	43
TTTACGA	20	7.85763E-4	44.0	43
ACGCATG	20	7.85763E-4	44.0	20
CACGATT	25	4.4438122E-5	44.0	22
TAACGTA	20	7.85763E-4	44.0	34
CGATTCA	35	1.4466787E-7	44.0	36
TCGATTG	30	2.5284899E-6	44.0	28
CGACAGT	20	7.85763E-4	44.0	22
ATGTACG	25	4.4438122E-5	44.0	42
CCGTACT	20	7.85763E-4	44.0	43
CTACGTA	20	7.85763E-4	44.0	11
CGTAGAT	25	4.4438122E-5	44.0	31
TACGAAT	25	4.4438122E-5	44.0	16
CGAATGT	45	4.802132E-10	44.0	24
CGTACTC	25	4.4438122E-5	44.0	15
CATACCG	20	7.85763E-4	44.0	17
>>END_MODULE