FastQCFastQC Report
Fri 17 Jun 2016
SRR1527167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1883064
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC58030.3081679645513907No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG50350.26738337093163056No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC49790.26440949431352306No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT49370.26217908684994246No Hit
AAAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG48370.2568685928890362No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA43760.2323872157292583No Hit
AAAACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC40140.21316322759077758No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT38030.20195808533326537No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC37060.19680690619118626No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG36180.19213367150558877No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC28920.15357948534940924No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC28920.15357948534940924No Hit
AAAACGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG28490.15129597294621958No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG26070.1384445775608264No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT24900.13223129962656607No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG24860.13201887986812982No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC23350.12400003398716135No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20740.11013964474919599No Hit
AAAACGGGGGCTGCATCCACACTGACTGAGAGGCCTTGCTATAACCGGAGAG19970.10605056439929815No Hit
AAAACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA19840.10536020018438036No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA19760.10493536066750785No Hit
AAAACGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG19670.10445741621102629No Hit
AAAACGGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGT19640.10429810139219911No Hit
AAAACGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT19010.10095249019682814No Hit
AAAACGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG18990.10084628031761002No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC206.3124864E-446.035
CGTATAG2000.046.032
TTATGCG253.4179935E-546.014
TAACGTA206.3124864E-446.034
ATCACGC206.3124864E-446.010
CGTTAGT301.8620412E-646.030
CGCCTAT301.8620412E-646.030
CATACTT950.046.037
GCGATAG206.3124864E-446.013
TCGTACA206.3124864E-446.041
CGTAATA253.4179935E-546.017
TATAGCG253.4179935E-546.046
CGTAACC253.4179935E-546.019
ATAACGT206.3124864E-446.033
CGCACAT600.046.023
TATCGCA301.8620412E-646.031
TACACGG405.6152203E-946.021
AATGCGA351.0200347E-745.99999646
ACGCTAA351.0200347E-745.99999628
CGTACAC351.0200347E-745.99999630
Produced by FastQC (version 0.11.5)