##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1883064
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32977636447832	33.0	31.0	34.0	31.0	34.0
2	32.93855068123017	34.0	31.0	34.0	31.0	34.0
3	33.20185984119499	34.0	34.0	34.0	31.0	34.0
4	36.561442415127686	37.0	37.0	37.0	35.0	37.0
5	36.35279151425549	37.0	37.0	37.0	35.0	37.0
6	36.25503806562071	37.0	37.0	37.0	35.0	37.0
7	36.59294054795801	37.0	37.0	37.0	35.0	37.0
8	36.61887009682093	37.0	37.0	37.0	35.0	37.0
9	38.601534520334944	39.0	39.0	39.0	38.0	39.0
10	38.01514181143073	39.0	38.0	39.0	37.0	39.0
11	36.870190816669	39.0	35.0	39.0	34.0	39.0
12	36.630817646134176	39.0	35.0	39.0	33.0	39.0
13	36.6421704201238	39.0	35.0	39.0	33.0	39.0
14	37.45311046252278	40.0	35.0	40.0	33.0	40.0
15	37.418327258128244	40.0	35.0	40.0	33.0	40.0
16	32.43352376764677	39.0	33.0	40.0	3.0	40.0
17	33.92237279242766	39.0	33.0	40.0	16.0	40.0
18	35.971853319908405	39.0	34.0	40.0	30.0	40.0
19	36.91098921757306	39.0	35.0	40.0	32.0	40.0
20	37.17043233793434	39.0	35.0	40.0	33.0	40.0
21	37.3615240905248	40.0	35.0	40.0	33.0	40.0
22	37.400133505818175	40.0	35.0	40.0	33.0	40.0
23	37.322020388048415	40.0	35.0	40.0	33.0	40.0
24	37.28444386383044	40.0	35.0	40.0	33.0	40.0
25	37.21342662809124	39.0	35.0	40.0	33.0	40.0
26	37.097896831971724	39.0	35.0	40.0	33.0	40.0
27	36.9320384224859	39.0	35.0	40.0	33.0	40.0
28	36.78307269428973	39.0	35.0	40.0	32.0	40.0
29	36.54046118453754	38.0	35.0	40.0	32.0	40.0
30	36.46804781993602	38.0	35.0	40.0	32.0	40.0
31	36.37677264288415	38.0	35.0	40.0	31.0	40.0
32	36.306649694327966	38.0	35.0	40.0	31.0	40.0
33	35.86731359104098	38.0	35.0	40.0	31.0	40.0
34	35.78464991099612	37.0	35.0	40.0	30.0	40.0
35	35.63321586520692	37.0	34.0	40.0	30.0	40.0
36	35.54631228678367	37.0	34.0	40.0	30.0	40.0
37	35.17387353802101	36.0	34.0	40.0	29.0	40.0
38	35.12631169200834	36.0	34.0	40.0	29.0	40.0
39	34.742809060127534	35.0	34.0	39.0	28.0	40.0
40	34.62689159794888	35.0	33.0	39.0	28.0	40.0
41	34.53037708755517	35.0	33.0	39.0	28.0	40.0
42	34.45950535935051	35.0	33.0	39.0	28.0	40.0
43	34.36055333222875	35.0	33.0	39.0	28.0	40.0
44	34.634165381527126	35.0	34.0	39.0	29.0	40.0
45	34.71569898845711	35.0	34.0	39.0	30.0	40.0
46	34.6617183483939	35.0	34.0	39.0	30.0	40.0
47	34.36922218257053	35.0	34.0	38.0	29.0	40.0
48	34.317847401893935	35.0	34.0	38.0	29.0	40.0
49	34.22376403563554	35.0	34.0	37.0	29.0	40.0
50	33.746524281702584	35.0	33.0	37.0	27.0	40.0
51	33.852103805287555	35.0	33.0	37.0	27.0	40.0
52	33.52540752730656	35.0	33.0	36.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	0.0
14	6.0
15	27.0
16	100.0
17	204.0
18	420.0
19	843.0
20	1432.0
21	2364.0
22	3792.0
23	5304.0
24	7523.0
25	10388.0
26	13598.0
27	17927.0
28	23027.0
29	29634.0
30	38396.0
31	51548.0
32	75403.0
33	122254.0
34	199231.0
35	221762.0
36	260152.0
37	338983.0
38	327597.0
39	131145.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.60872280496042	0.3197979463257754	0.04811307528581078	0.023366173427987576
8	99.11691795924091	0.6635462204152381	0.13212508974734793	0.08741073059651716
9	94.0991915303994	2.7898148974224988	1.6808775485060519	1.4301160236720578
10	55.28919888012304	29.425818771958895	6.119122876333465	9.165859471584609
11	34.82637871044213	22.44650208383783	20.89605026701164	21.831068938708402
12	28.242322087831322	16.723382742169147	28.61182625763118	26.422468912368352
13	24.409366861668005	17.442954673871945	30.144912759205212	28.002765705254838
14	24.301776254020044	17.871458431577473	31.725421971850132	26.10134334255235
15	25.649579621298052	18.959525539227556	27.494232803558454	27.89666203591593
16	28.488516587858935	27.479522735286743	21.721566553234517	22.310394123619805
17	29.990961539278537	19.878718938920823	23.091461575389896	27.038857946410744
18	29.983739267491703	19.602254623316043	22.653239613735913	27.76076649545634
19	28.87246530123246	19.69205507619497	22.499288393809238	28.936191228763334
20	28.79408241036948	21.442659410407718	23.900993274790448	25.86226490443235
21	30.38282288865381	18.93886771771963	24.921564004197414	25.756745389429142
22	30.94833739055072	18.652313463589127	24.7116932828624	25.687655862997754
23	29.720763606547628	20.295061665455876	23.38651261985785	26.597662108138653
24	29.899727253030168	20.671203952706865	22.137218915554648	27.29184987870832
25	29.105914615753896	20.60068059290603	22.01767969649465	28.275725094845424
26	28.506147427809147	20.634986383893484	21.691084317898916	29.167781870398457
27	29.568671059507274	18.56219438107255	22.88281226766589	28.98632229175429
28	30.026860478454264	19.993638028234834	21.64201535370014	28.337486139610764
29	29.136609270847934	19.864911654622468	22.841974569106522	28.156504505423076
30	28.899867450070737	20.34349337037934	23.41487065760909	27.341768521940836
31	30.810848701902856	20.776935887468508	21.338679938653176	27.07353547197546
32	32.61917810547066	19.7309278919888	21.684552410326997	25.96534159221354
33	32.067470887872105	20.703491755989177	22.192607367566904	25.03642998857182
34	29.142344604325714	21.972434287947728	24.295828500783827	24.589392606942727
35	29.948052748074417	23.215567819256275	23.767009512156783	23.069369920512525
36	30.834214875330844	21.725655633584413	22.21767289906238	25.222456592022365
37	31.36138761083001	22.390476372550268	21.059347956309505	25.18878806031022
38	30.970110415790437	22.392600570134633	21.915187163049158	24.722101851025773
39	31.832481530102	20.44311823708594	22.20981336800024	25.514586864811818
40	28.7045474821886	22.022777770697118	22.41825025596581	26.85442449114847
41	28.346460874404695	21.8668085630653	22.34236329726446	27.444367265265544
42	26.706049289880745	20.530157233105196	24.608138650624724	28.15565482638933
43	27.93192371581635	19.519517127405123	25.274605642718463	27.27395351406006
44	26.270482575207215	20.491443732130186	25.46764209819741	27.77043159446519
45	27.672028141369598	19.14687976616833	24.18887515241118	28.992216940050895
46	28.656381301963187	20.57577437622938	23.258742135158446	27.50910218664899
47	27.666876962227523	20.25502054099064	24.964579005280754	27.113523491501084
48	29.00798910711479	20.677895175097607	24.547174179953522	25.766941537834082
49	27.715096247392545	21.483178479329435	24.13327428064049	26.66845099263753
50	25.678150078807732	22.30147249376548	24.062644710960434	27.957732716466353
51	26.018871371339475	22.280761567317946	23.929988571816995	27.770378489525584
52	27.605965596495924	21.463848281311734	23.90556029959683	27.024625822595517
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	27.0
6	53.0
7	448.0
8	843.0
9	2061.0
10	3279.0
11	2562.0
12	1845.0
13	1846.5
14	2018.5
15	2189.0
16	2672.5
17	3156.0
18	3320.5
19	3485.0
20	3634.5
21	3784.0
22	4131.0
23	4478.0
24	5361.0
25	6244.0
26	7057.5
27	7871.0
28	9565.5
29	11260.0
30	12660.5
31	14061.0
32	17259.5
33	20458.0
34	21726.5
35	22995.0
36	23802.5
37	24610.0
38	25204.5
39	29203.5
40	32608.0
41	35314.5
42	38021.0
43	43323.0
44	48625.0
45	51972.5
46	55320.0
47	58248.0
48	61176.0
49	71485.5
50	81795.0
51	92680.0
52	103565.0
53	111821.0
54	120077.0
55	144375.0
56	168673.0
57	186923.5
58	205174.0
59	186813.5
60	168453.0
61	163095.5
62	157738.0
63	148646.5
64	125170.5
65	110786.0
66	91150.0
67	71514.0
68	64615.0
69	57716.0
70	50772.5
71	43829.0
72	40650.5
73	37472.0
74	26302.5
75	15133.0
76	9735.5
77	4338.0
78	3370.5
79	2403.0
80	1553.0
81	703.0
82	398.0
83	93.0
84	55.0
85	17.0
86	13.0
87	9.0
88	6.0
89	3.0
90	3.0
91	4.5
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1883064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.356192347797531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.00406172083623	6.7368385128282915
2	9.905451229806424	1.85354613995593
3	3.9774645336266725	1.1164176969946185
4	2.0719054320532715	0.7754058299494783
5	1.2279678659742277	0.5744551775484666
6	0.7785548087776699	0.4370585126535963
7	0.4793933160161631	0.3139707252426996
8	0.3602788980423144	0.26966709351491425
9	0.258223818182936	0.21743925405320222
>10	3.4320465486540677	8.699643877186451
>50	2.676089613839892	18.318836582797136
>100	2.6584966214917847	44.004042013077374
>500	0.11402865410810352	7.232735255824648
>1k	0.05473375397188968	8.789213006112995
>5k	0.0013031846183783257	0.6607303222601971
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5803	0.3081679645513907	No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	5035	0.26738337093163056	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4979	0.26440949431352306	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4937	0.26217908684994246	No Hit
AAAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4837	0.2568685928890362	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4376	0.2323872157292583	No Hit
AAAACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	4014	0.21316322759077758	No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3803	0.20195808533326537	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3706	0.19680690619118626	No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3618	0.19213367150558877	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2892	0.15357948534940924	No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2892	0.15357948534940924	No Hit
AAAACGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2849	0.15129597294621958	No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2607	0.1384445775608264	No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2490	0.13223129962656607	No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2486	0.13201887986812982	No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2335	0.12400003398716135	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2074	0.11013964474919599	No Hit
AAAACGGGGGCTGCATCCACACTGACTGAGAGGCCTTGCTATAACCGGAGAG	1997	0.10605056439929815	No Hit
AAAACGGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	1984	0.10536020018438036	No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1976	0.10493536066750785	No Hit
AAAACGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1967	0.10445741621102629	No Hit
AAAACGGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGT	1964	0.10429810139219911	No Hit
AAAACGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	1901	0.10095249019682814	No Hit
AAAACGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG	1899	0.10084628031761002	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.1862963765437604E-4	0.0	0.0	0.0	0.0
8	7.434691545268775E-4	0.0	0.0	0.0	0.0
9	0.0023366173427987577	0.0	0.0	0.0	0.0
10	0.011258247197121287	0.0	0.0	0.0	0.0
11	0.019595722715744127	0.0	0.0	0.0	0.0
12	0.025118636435086647	0.0	0.0	0.0	0.0
13	0.030216710637556665	0.0	0.0	0.0	0.0
14	0.04105011831780545	0.0	0.0	0.0	0.0
15	0.048909649379946726	0.0	0.0	0.0	0.0
16	0.06367282259126615	0.0	0.0	0.0	0.0
17	0.07179787835145274	0.0	0.0	0.0	0.0
18	0.08018845880968464	0.0	0.0	0.0	0.0
19	0.08841972444908935	0.0	0.0	0.0	0.0
20	0.09426126780608625	0.0	0.0	0.0	0.0
21	0.1007400704383919	0.0	0.0	0.0	0.0
22	0.10631608909734348	0.0	0.0	0.0	0.0
23	0.11465356461596632	0.0	0.0	0.0	0.0
24	0.12150410182553541	0.0	0.0	0.0	0.0
25	0.12840774397471355	0.0	0.0	0.0	0.0
26	0.13541759600310982	0.0	0.0	0.0	0.0
27	0.14348954682368736	0.0	0.0	0.0	0.0
28	0.15198633716113738	0.0	0.0	0.0	0.0
29	0.15931481882718804	0.0	0.0	0.0	0.0
30	0.16579362145949367	0.0	0.0	0.0	0.0
31	0.17290968336710807	0.0	0.0	0.0	0.0
32	0.1792822761201956	0.0	0.0	0.0	0.0
33	0.1851238194771925	0.0	0.0	0.0	0.0
34	0.19245230114324313	0.0	0.0	0.0	0.0
35	0.20567543110589975	0.0	0.0	0.0	0.0
36	0.22006686973995573	0.0	0.0	0.0	0.0
37	0.22882918477545108	0.0	0.0	0.0	0.0
38	0.23552040716619296	0.0	0.0	0.0	0.0
39	0.24311441353028893	0.0	0.0	0.0	0.0
40	0.2520891483242205	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAC	20	6.3124864E-4	46.0	35
CGTATAG	200	0.0	46.0	32
TTATGCG	25	3.4179935E-5	46.0	14
TAACGTA	20	6.3124864E-4	46.0	34
ATCACGC	20	6.3124864E-4	46.0	10
CGTTAGT	30	1.8620412E-6	46.0	30
CGCCTAT	30	1.8620412E-6	46.0	30
CATACTT	95	0.0	46.0	37
GCGATAG	20	6.3124864E-4	46.0	13
TCGTACA	20	6.3124864E-4	46.0	41
CGTAATA	25	3.4179935E-5	46.0	17
TATAGCG	25	3.4179935E-5	46.0	46
CGTAACC	25	3.4179935E-5	46.0	19
ATAACGT	20	6.3124864E-4	46.0	33
CGCACAT	60	0.0	46.0	23
TATCGCA	30	1.8620412E-6	46.0	31
TACACGG	40	5.6152203E-9	46.0	21
AATGCGA	35	1.0200347E-7	45.999996	46
ACGCTAA	35	1.0200347E-7	45.999996	28
CGTACAC	35	1.0200347E-7	45.999996	30
>>END_MODULE