FastQCFastQC Report
Fri 17 Jun 2016
SRR1527166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1828888
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC60350.32998193437761086No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT52530.2872237118948782No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG48430.26480571801006947No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC47810.26141567991041553No Hit
AAAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG46930.25660401293026147No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA43700.23894300799174145No Hit
AAAACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC37960.20755781655300926No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT37690.2060815096386438No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC36260.19826255079589344No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG34690.1896780994790277No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC29740.1626124727156611No Hit
AAAACGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG28400.15528561617769923No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC28230.15435608960198766No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG25990.14210821001614096No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG25480.1393196302890062No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT23170.12668900446610182No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC22400.122478795858467No Hit
AAAACGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG20130.11006688217102414No Hit
AAAACGGGGGCTGCATCCACACTGACTGAGAGGCCTTGCTATAACCGGAGAG20090.1098481700355626No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA20030.10952010183237026No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19200.10498182502154314No Hit
AAAACGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG18890.10328680597171615No Hit
AAAACGGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGT18500.10115436265096606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAC351.0200165E-746.00000445
TATTAGC351.0200165E-746.00000425
CGATATT351.0200165E-746.00000427
CACGCTA253.4179546E-546.027
TCGATTG301.8620121E-646.028
TCGATAG501.6370905E-1146.024
ATACTCC600.046.022
CGTTAGT301.8620121E-646.030
TCGGATA301.8620121E-646.015
ACGCTAA301.8620121E-646.028
GTATATC206.3124386E-446.023
TATAGCG206.3124386E-446.046
CGCACAT501.6370905E-1146.023
CCGTTAT206.3124386E-446.014
ATCGATT301.8620121E-646.011
AAAACGG1868450.044.851511
ACGGGGG977950.044.8311274
AAACGGG1855050.044.8270952
AACGGGG1743200.044.812533
ACGGGGA516100.044.752184
Produced by FastQC (version 0.11.5)