##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1828888
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25038985438146	33.0	31.0	34.0	31.0	34.0
2	32.900172126450606	34.0	31.0	34.0	31.0	34.0
3	33.16805567098696	34.0	34.0	34.0	31.0	34.0
4	36.56480167183556	37.0	37.0	37.0	35.0	37.0
5	36.3683784900989	37.0	37.0	37.0	35.0	37.0
6	36.2320464675803	37.0	37.0	37.0	35.0	37.0
7	36.59359895193145	37.0	37.0	37.0	35.0	37.0
8	36.64317333811584	37.0	37.0	37.0	35.0	37.0
9	38.54186587697005	39.0	39.0	39.0	38.0	39.0
10	38.00273936949666	39.0	38.0	39.0	35.0	39.0
11	36.77916198258177	39.0	35.0	39.0	34.0	39.0
12	36.56674820984117	39.0	35.0	39.0	33.0	39.0
13	36.53140924977363	39.0	35.0	39.0	33.0	39.0
14	37.37562934416979	40.0	35.0	40.0	33.0	40.0
15	37.53580536369641	40.0	35.0	40.0	33.0	40.0
16	37.644441321721175	40.0	35.0	40.0	33.0	40.0
17	37.601470948467046	40.0	35.0	40.0	33.0	40.0
18	37.58949864617188	40.0	35.0	40.0	33.0	40.0
19	37.5178162905547	40.0	35.0	40.0	33.0	40.0
20	37.42859486201451	40.0	35.0	40.0	33.0	40.0
21	33.09146432148934	38.0	33.0	40.0	10.0	40.0
22	33.71737635109422	38.0	33.0	40.0	16.0	40.0
23	35.72562234538145	38.0	34.0	40.0	28.0	40.0
24	36.58054675846744	38.0	35.0	40.0	31.0	40.0
25	36.90254788702206	39.0	35.0	40.0	33.0	40.0
26	36.97781766844115	39.0	35.0	40.0	33.0	40.0
27	36.89436695959512	39.0	35.0	40.0	33.0	40.0
28	36.748631955592685	39.0	35.0	40.0	32.0	40.0
29	36.663340784126746	39.0	35.0	40.0	32.0	40.0
30	36.52007230623198	38.0	35.0	40.0	32.0	40.0
31	36.33744986024295	38.0	35.0	40.0	31.0	40.0
32	36.299934714427565	38.0	35.0	40.0	31.0	40.0
33	35.88588584976226	37.0	35.0	40.0	31.0	40.0
34	35.77611313541343	37.0	35.0	40.0	30.0	40.0
35	35.63007521510338	37.0	34.0	40.0	30.0	40.0
36	35.482973807034654	37.0	34.0	40.0	30.0	40.0
37	35.327799187265704	36.0	34.0	40.0	30.0	40.0
38	35.21979476053208	36.0	34.0	40.0	30.0	40.0
39	35.03940864612814	35.0	34.0	40.0	30.0	40.0
40	30.236937964489897	35.0	29.0	39.0	8.0	40.0
41	30.96735502666101	35.0	29.0	38.0	12.0	40.0
42	32.688265219083945	35.0	30.0	38.0	23.0	40.0
43	33.54993854188994	35.0	32.0	38.0	26.0	40.0
44	34.31126947084786	35.0	33.0	39.0	28.0	40.0
45	34.587847369549145	35.0	34.0	39.0	29.0	40.0
46	34.63139404928022	35.0	34.0	39.0	30.0	40.0
47	34.57988406069699	35.0	34.0	38.0	30.0	40.0
48	34.43607645738832	35.0	34.0	38.0	29.0	40.0
49	34.283143637007846	35.0	34.0	37.0	29.0	40.0
50	34.18207293174869	35.0	34.0	37.0	29.0	40.0
51	34.047205733757345	35.0	34.0	37.0	29.0	40.0
52	33.6648564592255	35.0	33.0	36.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	3.0
14	12.0
15	37.0
16	116.0
17	218.0
18	448.0
19	865.0
20	1552.0
21	2566.0
22	3807.0
23	5759.0
24	7934.0
25	10775.0
26	14327.0
27	18758.0
28	24451.0
29	31872.0
30	42292.0
31	62106.0
32	89389.0
33	108901.0
34	214548.0
35	224130.0
36	241486.0
37	270929.0
38	317406.0
39	134199.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.61047368674298	0.3198664981125143	0.04439856349869429	0.025261251645808818
8	99.12198013218962	0.6770780933550878	0.11351159830454353	0.0874301761507539
9	94.04999103280245	2.7752929649054505	1.734332556176212	1.4403834461158913
10	55.31372068710605	29.42881138702862	6.089109885351099	9.168358040514237
11	34.93144468113958	22.3977630122785	20.85305387754745	21.81773842903447
12	28.241313847540145	16.715403020852015	28.603993246169257	26.439289885438583
13	24.48307386783663	17.53059782775107	30.112341488379823	27.873986816032474
14	24.303237814453375	17.855111958742143	31.763235364877456	26.07841486192703
15	25.605668581126896	18.73192890980749	27.56642287553967	28.095979633525946
16	30.003040098682916	19.418520981055153	24.633821207203503	25.944617713058427
17	30.022505478738992	19.889845632974794	23.05548508164524	27.032163806640973
18	29.984066820931627	19.59912252691253	22.623856682311875	27.79295396984397
19	28.834570514979596	19.736036323711456	22.439044927846865	28.990348233462083
20	28.797772198188188	21.486280187742494	23.867727274715563	25.84822033935375
21	34.796444615525935	18.49626658384767	23.678267887371998	23.029020913254392
22	31.06286442909571	18.61989361841731	24.714197916985622	25.603044035501355
23	29.72226839478415	20.369098599804907	23.316572693352462	26.592060312058475
24	29.978653695578956	20.66873422538723	22.0687106044766	27.283901474557215
25	29.063398086706236	20.65473664871769	22.021250071081443	28.260615193494626
26	28.505244717008367	20.6345057761875	21.67601296525539	29.184236541548746
27	29.525974253207416	18.602068579377196	22.833218873982442	29.038738293432946
28	29.997845685465702	20.072579622152915	21.63035680697779	28.29921788540359
29	29.01823403073343	19.91297444129985	22.844865295195767	28.223926232770953
30	28.87224368031285	20.39600019246668	23.425600692880046	27.30615543434043
31	30.862032010708145	20.826480353088872	21.248376062394197	27.063111573808783
32	32.640599096281456	19.755720415902996	21.695150277108276	25.90853021070727
33	32.08047731736443	20.67786545704275	22.13957333636614	25.102083889226677
34	29.166520858576362	22.048862478183466	24.2422171286596	24.542399534580575
35	30.034042543884592	23.200873973693305	23.775868177821717	22.989215304600393
36	30.875318772937437	21.74878942833022	22.21163898500072	25.164252813731625
37	31.288137928621108	22.369385112702364	21.027039381307112	25.31543757736942
38	31.030713745182865	22.38250784083006	21.880563489945803	24.706214924041277
39	31.636874428614547	20.325192138611005	22.231432433259993	25.80650099951446
40	28.920524384215984	26.659861074051555	20.55828459697915	23.861329944753315
41	28.3301656525714	21.88816373665309	22.297920922440305	27.48374968833521
42	26.75937509568656	20.470745064760663	24.570175975784192	28.199703863768583
43	27.964424284045826	19.48566560664185	25.35743030737804	27.19247980193429
44	26.28170779183854	20.46735502666101	25.491938270686887	27.758998910813563
45	27.70787494914943	19.1630105288022	24.117223143243326	29.011891378805043
46	28.645821942076278	20.602300414240784	23.293881309298328	27.45799633438461
47	27.56642287553967	20.248806925301057	24.862211354659223	27.322558844500044
48	29.020257117986446	20.638606628727402	24.62080783514354	25.720328418142607
49	27.74018966716387	21.415854880123877	24.145929111022653	26.698026341689594
50	25.599435285266235	22.19436072629926	24.140680019771576	28.065523968662927
51	26.09727878361059	22.27747133777465	23.875983657829238	27.74926622078553
52	27.589168937627672	21.505308143527653	23.8920043217518	27.01351859709288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	18.0
6	36.0
7	416.0
8	796.0
9	1920.5
10	3045.0
11	2450.0
12	1855.0
13	1933.0
14	2051.0
15	2091.0
16	2507.5
17	2924.0
18	3065.5
19	3207.0
20	3411.0
21	3615.0
22	3988.5
23	4362.0
24	5081.5
25	5801.0
26	6603.0
27	7405.0
28	9163.0
29	10921.0
30	12153.5
31	13386.0
32	16546.5
33	19707.0
34	20920.0
35	22133.0
36	22737.5
37	23342.0
38	24199.0
39	28412.0
40	31768.0
41	34005.0
42	36242.0
43	41190.0
44	46138.0
45	49992.0
46	53846.0
47	56263.0
48	58680.0
49	68732.5
50	78785.0
51	90004.0
52	101223.0
53	108023.5
54	114824.0
55	138092.0
56	161360.0
57	180670.5
58	199981.0
59	182273.5
60	164566.0
61	160002.0
62	155438.0
63	145355.0
64	122216.0
65	109160.0
66	90218.5
67	71277.0
68	63799.0
69	56321.0
70	48942.0
71	41563.0
72	39641.0
73	37719.0
74	26516.0
75	15313.0
76	9884.5
77	4456.0
78	3439.5
79	2423.0
80	1563.5
81	704.0
82	400.0
83	96.0
84	58.5
85	21.0
86	14.0
87	7.0
88	5.5
89	4.0
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1828888.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.518643676515397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.9137947347476	6.940404311826469
2	9.041865861767315	1.7213259861802381
3	3.8953111212290685	1.1123423571644153
4	2.0220198829186926	0.7698754708932967
5	1.210723425161266	0.5762222437460173
6	0.7378162273514893	0.421380586014582
7	0.5003170856355422	0.3333638044406157
8	0.35367063902572354	0.269317183338509
9	0.24959937186594897	0.2138262734406627
>10	3.5824955125829625	9.281619510358134
>50	2.778790806350894	19.30050117805169
>100	2.550606643894979	42.76105440781129
>500	0.11242893135582979	7.320222500607877
>1k	0.049229236214978725	8.263745989008028
>5k	0.0013305198977021278	0.7147981971181809
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6035	0.32998193437761086	No Hit
AAAACGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5253	0.2872237118948782	No Hit
AAAACGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4843	0.26480571801006947	No Hit
AAAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4781	0.26141567991041553	No Hit
AAAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4693	0.25660401293026147	No Hit
AAAACGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4370	0.23894300799174145	No Hit
AAAACGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	3796	0.20755781655300926	No Hit
AAAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	3769	0.2060815096386438	No Hit
AAAACGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3626	0.19826255079589344	No Hit
AAAACGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3469	0.1896780994790277	No Hit
AAAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2974	0.1626124727156611	No Hit
AAAACGGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG	2840	0.15528561617769923	No Hit
AAAACGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2823	0.15435608960198766	No Hit
AAAACGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2599	0.14210821001614096	No Hit
AAAACGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2548	0.1393196302890062	No Hit
AAAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2317	0.12668900446610182	No Hit
AAAACGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	2240	0.122478795858467	No Hit
AAAACGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	2013	0.11006688217102414	No Hit
AAAACGGGGGCTGCATCCACACTGACTGAGAGGCCTTGCTATAACCGGAGAG	2009	0.1098481700355626	No Hit
AAAACGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	2003	0.10952010183237026	No Hit
AAAACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1920	0.10498182502154314	No Hit
AAAACGGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTG	1889	0.10328680597171615	No Hit
AAAACGGGGGAGGAAGACGCAGTGGAATTCATCAGTGTGCCTGTACCAGAGT	1850	0.10115436265096606	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.2806820319232233E-4	0.0	0.0	0.0	0.0
8	9.84204609576967E-4	0.0	0.0	0.0	0.0
9	0.003116647930327062	0.0	0.0	0.0	0.0
10	0.0122478795858467	0.0	0.0	0.0	0.0
11	0.019848126293135503	0.0	0.0	0.0	0.0
12	0.02783111923748201	0.0	0.0	0.0	0.0
13	0.03444716133519384	0.0	0.0	0.0	0.0
14	0.045710836311463575	0.0	0.0	0.0	0.0
15	0.054295287628329345	0.0	0.0	0.0	0.0
16	0.0646294360288875	0.0	0.0	0.0	0.0
17	0.070917409923407	0.0	0.0	0.0	0.0
18	0.07676795954700343	0.0	0.0	0.0	0.0
19	0.08502434266067688	0.0	0.0	0.0	0.0
20	0.08967197553923477	0.0	0.0	0.0	0.0
21	0.09623333960308121	0.0	0.0	0.0	0.0
22	0.10274002563306228	0.0	0.0	0.0	0.0
23	0.10957477986623566	0.0	0.0	0.0	0.0
24	0.11733906067512062	0.0	0.0	0.0	0.0
25	0.12368171260350552	0.0	0.0	0.0	0.0
26	0.13073517897214046	0.0	0.0	0.0	0.0
27	0.1389915620858139	0.0	0.0	0.0	0.0
28	0.1487242521138528	0.0	0.0	0.0	0.0
29	0.15380930926333378	0.0	0.0	0.0	0.0
30	0.1609174536658341	0.0	0.0	0.0	0.0
31	0.1681349541360652	0.0	0.0	0.0	0.0
32	0.17431357196285394	0.0	0.0	0.0	0.0
33	0.18005476551871957	0.0	0.0	0.0	0.0
34	0.18683484171802756	0.0	0.0	0.0	0.0
35	0.2013791987262205	0.0	0.0	0.0	0.0
36	0.2124788396008941	0.0	0.0	0.0	0.0
37	0.2200790863081829	0.0	0.0	0.0	0.0
38	0.22778868908320246	0.0	0.0	0.0	0.0
39	0.2343500531470489	0.0	0.0	0.0	0.0
40	0.24315321659937622	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAC	35	1.0200165E-7	46.000004	45
TATTAGC	35	1.0200165E-7	46.000004	25
CGATATT	35	1.0200165E-7	46.000004	27
CACGCTA	25	3.4179546E-5	46.0	27
TCGATTG	30	1.8620121E-6	46.0	28
TCGATAG	50	1.6370905E-11	46.0	24
ATACTCC	60	0.0	46.0	22
CGTTAGT	30	1.8620121E-6	46.0	30
TCGGATA	30	1.8620121E-6	46.0	15
ACGCTAA	30	1.8620121E-6	46.0	28
GTATATC	20	6.3124386E-4	46.0	23
TATAGCG	20	6.3124386E-4	46.0	46
CGCACAT	50	1.6370905E-11	46.0	23
CCGTTAT	20	6.3124386E-4	46.0	14
ATCGATT	30	1.8620121E-6	46.0	11
AAAACGG	186845	0.0	44.85151	1
ACGGGGG	97795	0.0	44.831127	4
AAACGGG	185505	0.0	44.827095	2
AACGGGG	174320	0.0	44.81253	3
ACGGGGA	51610	0.0	44.75218	4
>>END_MODULE