FastQCFastQC Report
Fri 17 Jun 2016
SRR1527165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268461
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT31901.1882545323156808No Hit
GCGAATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTC22850.8511478389784735No Hit
GCGAATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCA22000.8194858843556421No Hit
GCGAATGGGCAGTGCTGCGAATGGGGTGTGTTTGTCTGGTATGATGTGCT16200.6034396057527909No Hit
GCGAATGGGGTTTTTTAAATACTTGTTTACAGTTACACCAAGATATTTTG15730.585932407314284No Hit
GCGAATGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCT13600.506591273965306No Hit
GCGAATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAG13180.49094654344578914No Hit
GCGAATGGGATTTGTGAATAAGCTGATCCCGTCACTCAACATGGAGCCTG13010.4846141525212228No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC9560.3561038661109062No Hit
GCGAATGGGGGTTGGGGATCGCTGTGCCATGAGGAACTGTGTTGAAGGGT8030.29911234778980933No Hit
GCGAATGGGATAAAAATTAATATAAAACAGTTGACCGTACATATGGTGAT7930.2953874119518291No Hit
GCGAATGGGGACAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGA7910.2946424247842331No Hit
GCGAATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA7380.2749002648429381No Hit
GCGAATGGGATTCATGTCCCATCTCTGTGCATCCTGACTGCGCACATCCC7290.27154782258875587No Hit
GCGAATGGGGATTTCTACATTCCTTTTCTGAACCTTCTTGGAGGTTTCCT7150.26633291241558366No Hit
GCGAATGGGGTTTTCCACTTACATCTGCACACGCACGCACCACGGTGCAC6750.25143316906366286No Hit
GCGAATGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATG6260.23318098345755994No Hit
GCGAATGGGCTGCCCGCCCTCCGCTACATTTATTACCCAAGGTTCTGATG6190.2305735283709738No Hit
GCGAATGGGATTTAGGAGTTCAGCATTAATTTCCAAAATTTTCATGGGGC6190.2305735283709738No Hit
GCGAATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAA6060.22573111178159957No Hit
GCGAATGGGGGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAGAACGA5960.22200617594361938No Hit
GCGAATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTG5680.21157635559727483No Hit
GCGAATGGGAACTCAAAATTTTTAATATTTTCATTAACTTGACTAATTAA5560.2071064325916986No Hit
GCGAATGGGGTGGAACAAGTGGATTCGAAGGGGGAAGAGCAAAGCTCAGG5340.1989115737481422No Hit
GCGAATGGGGTGGGAGAGGGACTGGATCCCGTCCTCTAACGTCTGTCTCT5260.19593162507775805No Hit
GCGAATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT5120.19071671490458578No Hit
GCGAATGGGACAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAA5080.1892267405693937No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5020.18699177906660558No Hit
GCGAATGGGCGGAAAGTGGGAAGAGTGCATTGCTATTCAGTTCACTTGGA4920.18326684322862538No Hit
GCGAATGGGATAAGCGAACTTTGTGCCTGTCCTACAAATGAACATTGTTC4830.17991440097444322No Hit
GCGAATGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT4740.17656195872026104No Hit
GCGAATGGGGAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAG4620.17209203571468482No Hit
GCGAATGGGGCTCCCAATCCTAATCTCAGCACTAGAATAGTGAAACATGG4610.1717195421308868No Hit
GCGAATGGGAGCTACTGACACTGCCCCCAGCTTTGCTTCTCACCAGCTCA4530.16873959346050263No Hit
GCGAATGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGT4480.16687712554151254No Hit
GCGAATGGGAATAAAGTTACTGCTTAAAGTTCAATAAATTTACGATGGCC4480.16687712554151254No Hit
GCGAATGGGAACACCGGGCACCCACTATGCCTAAGTCCTCACCTTCCCAC4470.16650463195771453No Hit
GCGAATGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAA4460.16613213837391652No Hit
GCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA4410.1642696704549264No Hit
GCGAATGGGGAGCCAATTTTGATAATGTTTCTCGTGGAAAAAAATCAGTA4240.15793727953036008No Hit
GCGAATGGGAGAGAGCGAGAGCGGGACCGGGACAGAGAAAGGGAGCGCAC4230.15756478594656206No Hit
GCGAATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA4230.15756478594656206No Hit
GCGAATGGGAAGCGCTCGCACGCGCCTTCCTCACCGGAGACGGGGCAGGT4120.15346735652478385No Hit
GCGAATGGGGGCTCGTGGTCCCAAGAAACACCTGAAGCGTGTGGCGGCCC4100.1527223693571878No Hit
GCGAATGGGGAAATCAAGTCAGTATGAATGGTGAGATGTCTTTGAAATCC3990.1486249399354096No Hit
GCGAATGGGGCCCACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCCG3970.14787995276781357No Hit
GCGAATGGGCTATTCTCTTTGCTTCTGTCGCTCATCGCAGACACCAGCCG3930.1463899784326215No Hit
GCGAATGGGAGTGTCCCTCTTAGTGGATGTGTTGGCAACCTTAATAAATC3900.14527249768122744No Hit
GCGAATGGGACTTTACCTACCGTATGCTAAGGCCCGCCCCTCACCCCTAC3890.14490000409742942No Hit
GCGAATGGGATTGCTGGCCACCCCTCTCTGTCAATCTGTGCGCTCTTTTG3830.1426650425946413No Hit
GCGAATGGGATCCCCAGAGAGGAGAGAAAGCACTAAGTGTGTATATGGGG3780.14080257467565122No Hit
GCGAATGGGAACTATGCCCGCCATCTCACCAAGTAGGCGCTCACAGCCTG3770.1404300810918532No Hit
GCGAATGGGAGCTTATAAATTATAGAAGGTTTTACTTTTTGTTTTCTATT3720.1385676131728631No Hit
GCGAATGGGGTGACCTTCTGTACGGATTTGCCCTCCCGGAAGGAATTCTG3710.13819511958906508No Hit
GCGAATGGGAGCACTGTTGGTAACCGGTACTACTTTATTAATGATTTTCT3710.13819511958906508No Hit
GCGAATGGGACCCCACCCCACCCCTGCTCTGATGGGCCAGAGGCTTTTGG3630.13521517091868093No Hit
GCGAATGGGCTCTTCGGCAGTCCTCCTTCCCAACATGGCGCAGTCGATTA3600.13409769016728687No Hit
GCGAATGGGGGGACCATTACTAAGTGTATTTCTCTAGCATATTATGTTTA3570.1329802094158928No Hit
GCGAATGGGGTTCGGCTGAAGCCTACCAGAAAGTTTGCTTACCTGGGGCG3570.1329802094158928No Hit
GCGAATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC3550.13223522224829679No Hit
GCGAATGGGATCCCTGGCAAGACACCATCCTGAAGGCACTGGGACTTTGC3550.13223522224829679No Hit
GCGAATGGGGTTTTGGATGTTGCTATAGTGACATGCAGTTCTATATTTTG3510.13074524791310468No Hit
GCGAATGGGATAGTTGGAGTCGAGGTCAAACCTCTCGTGGAACCTGCCTC3450.1285102864103166No Hit
GCGAATGGGAATAGAGGGAATATTAATTAAGTGATCTCTAAACTGTGACA3410.12702031207512451No Hit
GCGAATGGGGACTTCAGACTCCACATCTTGCAAAGTTTAACATCGGAGGG3400.1266478184913265No Hit
GCGAATGGGAAGTGTTGCTGAAAAGCATGGTGCTGGTAACAGTTCAACAA3390.12627532490752846No Hit
GCGAATGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT3360.1251578441561344No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG3280.12217789548575025No Hit
GCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC3280.12217789548575025No Hit
GCGAATGGGGAAGATGGCACTAGAGCGAGAAGTGGAGCGTTCTAACTGTC3280.12217789548575025No Hit
GCGAATGGGGGTCTTTTCTTCCCAGCAACTCTGTTGCACAAGGATTCCAG3270.12180540190195224No Hit
GCGAATGGGGACTTCTGGCCCTGGCCCTGACCTGCTTTCCCAACAGCTTT3230.12031542756676017No Hit
GCGAATGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTG3230.12031542756676017No Hit
GCGAATGGGGCTCTGCGTTCTGGAGTGGCGCTGCTTGGTCCGGCGGCTGG3180.11845295964777007No Hit
GCGAATGGGAAATTTTCAAAATGCAGTAGCTACCACATGGCTATCTGGTA3180.11845295964777007No Hit
GCGAATGGGGCAGGGATCAGTTTAATTTCTAATCCTCAACAAAACCTTAT3180.11845295964777007No Hit
GCGAATGGGACCTGGGCCAGGGATGGGTTTCCTGGTGTCACTGACATACT3160.11770797248017402No Hit
GCGAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGC3150.11733547889637601No Hit
GCGAATGGGCTAGAGTGGGAGACATGGCTCCCCTGTTCCCTCCTTCCCTC3140.11696298531257798No Hit
GCGAATGGGTATTTTAAATAGTGTGTGCTTTATGATTTGTGAAAGTCTAT3110.11584550456118392No Hit
GCGAATGGGGGTTTTTTTTCTTTCTGTGAAATGGGTATTATTATCCCCAA3100.11547301097738591No Hit
GCGAATGGGAGCCCTGCTGTTGGGGTGGACTTTGAGCTTGAGTCTTTCTC3100.11547301097738591No Hit
GCGAATGGGGGGTGTGCGAGGCGCGGGCTGTTGTGCTCCCGGCTCTGCTC3060.11398303664219385No Hit
GCGAATGGGGTCACCTGATGGATGGCCCTACTGTGAAGGAAGAAAAAAGT3050.11361054305839581No Hit
GCGAATGGGGCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCG3050.11361054305839581No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA3030.11286555589079979No Hit
GCGAATAGGCAGCACATGGCTACTAATGAATTACTTTAATTATTGCAAAT2990.1113755815556077No Hit
GCGAATGGGACTTACAAAGTTTGTTTTATTTAATTTCCCCTTTTCCTGAT2990.1113755815556077No Hit
GCGAATGGGAGCCACCTCTGTATTTATGTTGCTTATTCGTCTGTTTTTAT2990.1113755815556077No Hit
GCGAATGGGACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAG2980.11100308797180969No Hit
GCGAATGGGACTAGCTGCCTCGGCAGCGCGTTGCTTTCCCAAAATGGCTG2920.10876812646902158No Hit
GCGAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA2920.10876812646902158No Hit
GCGAATGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA2920.10876812646902158No Hit
GCGAATGGGGGATTTTGGCAACTTCGGGAATAAATGGCAATTCTCAAAAA2900.10802313930142553No Hit
GCGAATGGGATCCCCTGAGGGCACACTTACCGTGGAGGCTCCGTTGCCCA2900.10802313930142553No Hit
GCGAATGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC2900.10802313930142553No Hit
GCGAATGGGATTTATTTTATTTCATAAACTGTACATTTTTCTTAATGTTG2870.10690565855003148No Hit
GCGAATGGGGGTGCCTGCACACCGGGCACTTGGAGACTAATCCTGCCCTG2830.1054156842148394No Hit
GCGAATGGGGTCCAGGGCTTTCACGCGCAAGTGTGCGCGTGGGTCCTGAC2810.10467069704724337No Hit
GCGAATGGGGTGATGTTGGACTGTTTGATGTCTCCTCCTATTCCGTATCT2800.10429820346344533No Hit
GCGAATGGGGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATCC2800.10429820346344533No Hit
GCGAATGGGGTGCTGCGAATGGGGACTAGCTCATGCTCGCCGCAGGGGTC2750.10243573554445526No Hit
GCGAATGGGGACGTTTGCTTCTGATTCTGTTGTGTTGACTTGCAACCTCA2750.10243573554445526No Hit
GCGAATGGGAGTGGGGCCATGCTCTCGAACCAGTGAGGACATGGAGTTTG2730.10169074837685921No Hit
GCGAATGGGATCCGCAAGCCTGTGACTGTCCATTCCCGGGCTCGTTGCCG2720.1013182547930612No Hit
GCGAATGGGGATTCAAGAAATAACACAGAGACTTTTCTTCTTGCAAGTTA2720.1013182547930612No Hit
GCGAATGGGGGGAATGTCCAGATCTGTGTTTCCCTGCCCTCCCAGACTCT2710.10094576120926316No Hit
GCGAATGGGATTGCCCCTCAGGGACTTCAGGGAAGTTTTGCTCCAGACTA2700.10057326762546515No Hit
GCGAATGGGGAAGGCTTTGGTCTCCCTGGGAGGGGGTTGAGGTGTTGAGG2700.10057326762546515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGGA650.044.00000438
TCATTAA650.044.00000431
CCTTTGG650.044.00000417
AGGTCAA254.4336986E-544.023
TCACGCT207.8456925E-444.043
TCACGCG454.765752E-1044.021
TCACGAG254.4336986E-544.037
ACAACTA254.4336986E-544.016
TCGCCGT254.4336986E-544.015
TATCATT207.8456925E-444.035
TATCATG207.8456925E-444.017
TTCTATT551.8189894E-1244.044
ACAACGT408.270945E-944.019
TCGCCAG302.5204445E-644.014
ATTTACG551.8189894E-1244.038
GTTGAAG850.044.041
GTCATCG302.5204445E-644.034
ATCCTAA502.7284841E-1144.017
AGACTTT454.765752E-1044.029
CTTCGAT454.765752E-1044.018
Produced by FastQC (version 0.11.5)