FastQCFastQC Report
Fri 17 Jun 2016
SRR1527164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences288904
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT29231.0117547697505054No Hit
GCGAATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC21520.7448841137540497No Hit
GCGAATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA19650.6801567302633401No Hit
GCGAATGGGGTTTTTTAAATACTTGTTTACAGTTACACCAAGATATTTTGAA15340.5309722260681748No Hit
GCGAATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA14540.5032813668208125No Hit
GCGAATGGGATTTGTGAATAAGCTGATCCCGTCACTCAACATGGAGCCTGTG12910.44686124110431147No Hit
GCGAATGGGCAGTGCTGCGAATGGGGTGTGTTTGTCTGGTATGATGTGCTGT12750.441323069254839No Hit
GCGAATGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA12720.44028466203306293No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA9980.34544346911084656No Hit
GCGAATGGGGACAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAG7800.26998587766178384No Hit
GCGAATGGGATAAAAATTAATATAAAACAGTTGACCGTACATATGGTGATCC7660.2651399772934954No Hit
GCGAATGGGATTCATGTCCCATCTCTGTGCATCCTGACTGCGCACATCCCAT7300.2526790906321823No Hit
GCGAATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG7120.24644864730152577No Hit
GCGAATGGGGGTTGGGGATCGCTGTGCCATGAGGAACTGTGTTGAAGGGTCA6930.2398720682302772No Hit
GCGAATGGGGATTTCTACATTCCTTTTCTGAACCTTCTTGGAGGTTTCCTTT6440.2229114169412677No Hit
GCGAATGGGATTTAGGAGTTCAGCATTAATTTCCAAAATTTTCATGGGGCTT6270.21702710935120315No Hit
GCGAATGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC6250.21633483787001911No Hit
GCGAATGGGGTTTTCCACTTACATCTGCACACGCACGCACCACGGTGCACAT6170.21356575194528285No Hit
GCGAATGGGCTGCCCGCCCTCCGCTACATTTATTACCCAAGGTTCTGATGGT5940.20560462991166617No Hit
GCGAATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT5680.19660510065627337No Hit
GCGAATGGGAACTCAAAATTTTTAATATTTTCATTAACTTGACTAATTAAAC5580.19314374325035305No Hit
GCGAATGGGGGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAGAACGAGA5360.1855287569573284No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC5210.18033672084844793No Hit
GCGAATGGGGTGGGAGAGGGACTGGATCCCGTCCTCTAACGTCTGTCTCTGG5160.17860604214548778No Hit
GCGAATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG5060.17514468473956746No Hit
GCGAATGGGACAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAGC5010.1734140060366073No Hit
GCGAATGGGGTGGAACAAGTGGATTCGAAGGGGGAAGAGCAAAGCTCAGGTA4920.17029878437127904No Hit
GCGAATGGGGAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAGCA4830.16718356270595075No Hit
GCGAATGGGCGGAAAGTGGGAAGAGTGCATTGCTATTCAGTTCACTTGGATC4740.16406834104062248No Hit
GCGAATGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC4740.16406834104062248No Hit
GCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG4730.16372220530003048No Hit
GCGAATGGGGAAATCAAGTCAGTATGAATGGTGAGATGTCTTTGAAATCCAT4730.16372220530003048No Hit
GCGAATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC4370.15126131863871736No Hit
GCGAATGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA4260.14745382549220504No Hit
GCGAATGGGACTTTACCTACCGTATGCTAAGGCCCGCCCCTCACCCCTACTT4220.1460692825298369No Hit
GCGAATGGGATAAGCGAACTTTGTGCCTGTCCTACAAATGAACATTGTTCAG4200.14537701104865283No Hit
GCGAATGGGAAGCGCTCGCACGCGCCTTCCTCACCGGAGACGGGGCAGGTGC4160.14399246808628471No Hit
GCGAATGGGAGTGTCCCTCTTAGTGGATGTGTTGGCAACCTTAATAAATCTA4000.13845429623681224No Hit
GCGAATGGGAACACCGGGCACCCACTATGCCTAAGTCCTCACCTTCCCACTG3980.13776202475562815No Hit
GCGAATGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC3970.13741588901503612No Hit
GCGAATGGGGACTTCAGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT3770.13049317420319553No Hit
GCGAATGGGATTGCTGGCCACCCCTCTCTGTCAATCTGTGCGCTCTTTTGAT3750.12980090272201145No Hit
GCGAATGGGAGCTTATAAATTATAGAAGGTTTTACTTTTTGTTTTCTATTAT3700.12807022401905133No Hit
GCGAATAGGCAGCACATGGCTACTAATGAATTACTTTAATTATTGCAAATCA3690.1277240882784593No Hit
GCGAATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA3640.12599340957549912No Hit
GCGAATGGGATCCCCAGAGAGGAGAGAAAGCACTAAGTGTGTATATGGGGAG3570.12357045939135491No Hit
GCGAATGGGAGCACTGTTGGTAACCGGTACTACTTTATTAATGATTTTCTGT3570.12357045939135491No Hit
GCGAATGGGGGCTCGTGGTCCCAAGAAACACCTGAAGCGTGTGGCGGCCCCC3560.12322432365076288No Hit
GCGAATGGGGCCCACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCCGCG3510.12149364494780272No Hit
GCGAATGGGGAAGATGGCACTAGAGCGAGAAGTGGAGCGTTCTAACTGTCCA3480.12045523772602665No Hit
GCGAATGGGGTGACCTTCTGTACGGATTTGCCCTCCCGGAAGGAATTCTGGT3440.11907069476365853No Hit
GCGAATGGGCTATTCTCTTTGCTTCTGTCGCTCATCGCAGACACCAGCCGAC3380.11699388032010632No Hit
GCGAATGGGAGCCCTGCTGTTGGGGTGGACTTTGAGCTTGAGTCTTTCTCTG3380.11699388032010632No Hit
GCGAATGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGAG3260.11284025143300197No Hit
GCGAATGGGGTGATGTTGGACTGTTTGATGTCTCCTCCTATTCCGTATCTCT3250.11249411569240993No Hit
GCGAATGGGCTCTTCGGCAGTCCTCCTTCCCAACATGGCGCAGTCGATTAAC3250.11249411569240993No Hit
GCGAATGGGAATAGAGGGAATATTAATTAAGTGATCTCTAAACTGTGACACA3250.11249411569240993No Hit
GCGAATGGGATCCCTGGCAAGACACCATCCTGAAGGCACTGGGACTTTGCTG3230.11180184421122588No Hit
GCGAATGGGGTTCGGCTGAAGCCTACCAGAAAGTTTGCTTACCTGGGGCGTC3220.11145570847063385No Hit
GCGAATGGGGCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCGGC3190.11041730124885776No Hit
GCGAATGGGGCAGGGATCAGTTTAATTTCTAATCCTCAACAAAACCTTATAT3190.11041730124885776No Hit
GCGAATGGGGATTCAAGAAATAACACAGAGACTTTTCTTCTTGCAAGTTAAA3170.10972502976767369No Hit
GCGAATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG3160.10937889402708166No Hit
GCGAATGGGGACTTCAGACTCCACATCTTGCAAAGTTTAACATCGGAGGGGC3140.1086866225458976No Hit
GCGAATGGGAGCTACTGACACTGCCCCCAGCTTTGCTTCTCACCAGCTCATT3100.10730207958352948No Hit
GCGAATGGGAAGTGTTGCTGAAAAGCATGGTGCTGGTAACAGTTCAACAATC3090.10695594384293745No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC3080.10660980810234541No Hit
GCGAATGGGATCCCCTGAGGGCACACTTACCGTGGAGGCTCCGTTGCCCAAA3060.10591753662116137No Hit
GCGAATGGGCTAGAGTGGGAGACATGGCTCCCCTGTTCCCTCCTTCCCTCAA3030.10487912939938526No Hit
GCGAATGGGAACTATGCCCGCCATCTCACCAAGTAGGCGCTCACAGCCTGGC3030.10487912939938526No Hit
GCGAATGGGGTTTTGGATGTTGCTATAGTGACATGCAGTTCTATATTTTGTT3030.10487912939938526No Hit
GCGAATGGGGCTCTGCGTTCTGGAGTGGCGCTGCTTGGTCCGGCGGCTGGGT3020.10453299365879322No Hit
GCGAATGGGATAGTTGGAGTCGAGGTCAAACCTCTCGTGGAACCTGCCTCAA2990.10349458643701713No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG2990.10349458643701713No Hit
GCGAATGGGAGCATGACCTTTGGCCTTTGTAAGACACACAGCCTTTCTGTAC2950.10211004347464901No Hit
GCGAATGGGGGTTTTTTTTCTTTCTGTGAAATGGGTATTATTATCCCCAAGG2930.10141777199346497No Hit
GCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2930.10141777199346497No Hit
GCGAATGGGATCCGCAAGCCTGTGACTGTCCATTCCCGGGCTCGTTGCCGGA2910.1007255005122809No Hit
GCGAATGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT2900.10037936477168886No Hit
GCGAATGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCT2900.10037936477168886No Hit
GCGAATGGGAGTGGGGCCATGCTCTCGAACCAGTGAGGACATGGAGTTTGTT2890.10003322903109685No Hit
GCGAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG2890.10003322903109685No Hit
GCGAATGGGACATCTATGTAGGTGAAGCCCTGTGGCCCTAGGTGTGACCTAG2890.10003322903109685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT206.303572E-446.00000443
TCCGGAA206.303572E-446.00000411
TCACGCA206.303572E-446.00000417
ACAACGG405.5861165E-946.00000426
GTCATGA405.5861165E-946.00000417
CGGGTTG206.303572E-446.00000411
TTTAGAT206.303572E-446.00000421
TAATATC206.303572E-446.00000420
AGGTATA206.303572E-446.00000429
GAAATAG206.303572E-446.00000416
CAGCGAG206.303572E-446.00000443
GTCGCTA206.303572E-446.00000427
GGTACTC206.303572E-446.0000048
TCGCATG206.303572E-446.00000444
AATCCGG405.5861165E-946.00000437
CTCACGC206.303572E-446.00000416
GTACCGT206.303572E-446.00000427
ATCTCCG206.303572E-446.00000410
ATCTCCC206.303572E-446.00000436
GTACCCC206.303572E-446.00000434
Produced by FastQC (version 0.11.5)