FastQCFastQC Report
Fri 17 Jun 2016
SRR1527163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281547
Sequences flagged as poor quality0
Sequence length52
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAATGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCT29091.03322003075863No Hit
GCGAATGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTC19880.7060988041073071No Hit
GCGAATGGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGA19870.7057436236223437No Hit
GCGAATGGGGTTTTTTAAATACTTGTTTACAGTTACACCAAGATATTTTGAA15190.5395191566594565No Hit
GCGAATGGGATTTGTGAATAAGCTGATCCCGTCACTCAACATGGAGCCTGTG13130.4663519767569891No Hit
GCGAATGGGCAGTGCTGCGAATGGGGTGTGTTTGTCTGGTATGATGTGCTGT12580.4468170500840002No Hit
GCGAATGGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGA12440.44184452329451207No Hit
GCGAATGGGACATTTGCTTCTGACATAGTTGTGTTGACTCACAACCCCAGAA12390.44006862086969495No Hit
GCGAATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA9340.3317385729558475No Hit
GCGAATGGGGACAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAG7800.27704077827147866No Hit
GCGAATGGGGGTTGGGGATCGCTGTGCCATGAGGAACTGTGTTGAAGGGTCA7300.25928175402330694No Hit
GCGAATGGGATTCATGTCCCATCTCTGTGCATCCTGACTGCGCACATCCCAT7240.25715067111352635No Hit
GCGAATGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG6950.24685043704958673No Hit
GCGAATGGGATAAAAATTAATATAAAACAGTTGACCGTACATATGGTGATCC6550.2326432176510494No Hit
GCGAATGGGGATTTCTACATTCCTTTTCTGAACCTTCTTGGAGGTTTCCTTT6210.22056708116229262No Hit
GCGAATGGGGTTTTCCACTTACATCTGCACACGCACGCACCACGGTGCACAT6140.21808081776754856No Hit
GCGAATGGGGCTCTTCCGGTCTCTTTGGCCTCGCCGGTAGAAGCAAGATGAC5840.20742540321864555No Hit
GCGAATGGGATTTAGGAGTTCAGCATTAATTTCCAAAATTTTCATGGGGCTT5640.20032179351937687No Hit
GCGAATGGGGCTCTTTGGCCTCGCCGGTAGAAGCAAGATGACGAAGGGAACG5570.19783553012463284No Hit
GCGAATGGGCTGCCCGCCCTCCGCTACATTTATTACCCAAGGTTCTGATGGT5460.19392854479003505No Hit
GCGAATGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCT5340.18966637897047384No Hit
GCGAATGGGGTGGAACAAGTGGATTCGAAGGGGGAAGAGCAAAGCTCAGGTA5280.18753529606069325No Hit
GCGAATGGGGGGAGTGAGACGTCGAGCTGAGCGATCGCAGCGGAGAACGAGA5230.18575939363587607No Hit
GCGAATGGGAACTCAAAATTTTTAATATTTTCATTAACTTGACTAATTAAAC5210.1850490326659492No Hit
GCGAATGGGCGGAAAGTGGGAAGAGTGCATTGCTATTCAGTTCACTTGGATC4880.17332807666215588No Hit
GCGAATGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG4870.17297289617719244No Hit
GCGAATGGGACAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAGC4870.17297289617719244No Hit
GCGAATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4820.17119699375237526No Hit
GCGAATGGGGTGGGAGAGGGACTGGATCCCGTCCTCTAACGTCTGTCTCTGG4760.16906591084259465No Hit
GCGAATGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC4710.1672900084177775No Hit
GCGAATGGGGAGTTCACTCACTTTGAAAGCCAGCTGAAGGCAAGAGGAAGCA4690.16657964744785062No Hit
GCGAATGGGATAAGCGAACTTTGTGCCTGTCCTACAAATGAACATTGTTCAG4540.1612519401733991No Hit
GCGAATGGGAGTGTCCCTCTTAGTGGATGTGTTGGCAACCTTAATAAATCTA4310.15308278901924013No Hit
GCGAATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC4280.15201724756434984No Hit
GCGAATGGGGCTCTTTCTCTTCAACGAGGCGGCCGAGCGGCAGACGCCAACA4150.1473999012598252No Hit
GCGAATGGGAAGCGCTCGCACGCGCCTTCCTCACCGGAGACGGGGCAGGTGC4030.14313773544026398No Hit
GCGAATGGGAACACCGGGCACCCACTATGCCTAAGTCCTCACCTTCCCACTG3990.14171701350041022No Hit
GCGAATGGGATTGCTGGCCACCCCTCTCTGTCAATCTGTGCGCTCTTTTGAT3920.13923075010566618No Hit
GCGAATGGGGGACACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC3850.13674448671092215No Hit
GCGAATGGGGAAATCAAGTCAGTATGAATGGTGAGATGTCTTTGAAATCCAT3800.13496858428610498No Hit
GCGAATGGGACTTTACCTACCGTATGCTAAGGCCCGCCCCTCACCCCTACTT3730.13248232089136094No Hit
GCGAATGGGGGCTCGTGGTCCCAAGAAACACCTGAAGCGTGTGGCGGCCCCC3730.13248232089136094No Hit
GCGAATGGGCTATTCTCTTTGCTTCTGTCGCTCATCGCAGACACCAGCCGAC3610.12822015507179974No Hit
GCGAATGGGGCCCACTGTGGCCGCGGCGCGGGAAGGACAGTGAGCGGCCGCG3500.12431316973720197No Hit
GCGAATGGGGTTCGGCTGAAGCCTACCAGAAAGTTTGCTTACCTGGGGCGTC3490.12395798925223853No Hit
GCGAATGGGATCCCTGGCAAGACACCATCCTGAAGGCACTGGGACTTTGCTG3430.12182690634245794No Hit
GCGAATGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA3430.12182690634245794No Hit
GCGAATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC3370.11969582343267732No Hit
GCGAATGGGAGCACTGTTGGTAACCGGTACTACTTTATTAATGATTTTCTGT3320.11791992100786014No Hit
GCGAATGGGGTGACCTTCTGTACGGATTTGCCCTCCCGGAAGGAATTCTGGT3240.11507847712815267No Hit
GCGAATGGGAGCTTATAAATTATAGAAGGTTTTACTTTTTGTTTTCTATTAT3230.11472329664318924No Hit
GCGAATGGGGGTTTTTTTTCTTTCTGTGAAATGGGTATTATTATCCCCAAGG3170.11259221373340862No Hit
GCGAATGGGAACTATGCCCGCCATCTCACCAAGTAGGCGCTCACAGCCTGGC3170.11259221373340862No Hit
GCGAATGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG3160.1122370332484452No Hit
GCGAATGGGACCTGGGCCAGGGATGGGTTTCCTGGTGTCACTGACATACTAA3150.11188185276348177No Hit
GCGAATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG3120.11081631130859147No Hit
GCGAATGGGGCTGAGAGGTGGCATGCAGATCTTCGTGAAGACCCTGACCGGC3100.11010595033866459No Hit
GCGAATGGGATCCCCAGAGAGGAGAGAAAGCACTAAGTGTGTATATGGGGAG3090.10975076985370116No Hit
GCGAATGGGCTAGAGTGGGAGACATGGCTCCCCTGTTCCCTCCTTCCCTCAA3090.10975076985370116No Hit
GCGAATAGGCAGCACATGGCTACTAATGAATTACTTTAATTATTGCAAATCA3080.10939558936873774No Hit
GCGAATGGGATCCCCTGAGGGCACACTTACCGTGGAGGCTCCGTTGCCCAAA3080.10939558936873774No Hit
GCGAATGGGGTCACCTGATGGATGGCCCTACTGTGAAGGAAGAAAAAAGTTG3070.10904040888377428No Hit
GCGAATGGGAAGTGTTGCTGAAAAGCATGGTGCTGGTAACAGTTCAACAATC3060.10868522839881085No Hit
GCGAATGGGATAGTTGGAGTCGAGGTCAAACCTCTCGTGGAACCTGCCTCAA3050.10833004791384743No Hit
GCGAATGGGCTCTTCGGCAGTCCTCCTTCCCAACATGGCGCAGTCGATTAAC3040.10797486742888397No Hit
GCGAATGGGACTTGGAGGATTCTGGGTGGAAACTTCAGTCATCTGATCTGAG3030.10761968694392055No Hit
GCGAATGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT3030.10761968694392055No Hit
GCGAATGGGACTAGCTGCCTCGGCAGCGCGTTGCTTTCCCAAAATGGCTGCC3020.10726450645895712No Hit
GCGAATGGGGAAGATGGCACTAGAGCGAGAAGTGGAGCGTTCTAACTGTCCA3010.10690932597399369No Hit
GCGAATGGGAAATTTTCAAAATGCAGTAGCTACCACATGGCTATCTGGTAAC2980.10584378451910338No Hit
GCGAATGGGGCAGGGATCAGTTTAATTTCTAATCCTCAACAAAACCTTATAT2980.10584378451910338No Hit
GCGAATGGGAGCTACTGACACTGCCCCCAGCTTTGCTTCTCACCAGCTCATT2970.10548860403413994No Hit
GCGAATGGGAGCCCTGCTGTTGGGGTGGACTTTGAGCTTGAGTCTTTCTCTG2960.10513342354917651No Hit
GCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT2960.10513342354917651No Hit
GCGAATGGGGTGATGTTGGACTGTTTGATGTCTCCTCCTATTCCGTATCTCT2950.10477824306421309No Hit
GCGAATGGGAATGTTGCTTTTCTTGATGGTTAATAATAAATACTGTCACGTA2950.10477824306421309No Hit
GCGAATGGGGATTCAAGAAATAACACAGAGACTTTTCTTCTTGCAAGTTAAA2940.10442306257924966No Hit
GCGAATGGGGACTTCTGGCCCTGGCCCTGACCTGCTTTCCCAACAGCTTTGG2930.1040678820942862No Hit
GCGAATGGGGCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGCTCAGGCTA2890.10264716015443247No Hit
GCGAATGGGAATAGAGGGAATATTAATTAAGTGATCTCTAAACTGTGACACA2890.10264716015443247No Hit
GCGAATGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG2890.10264716015443247No Hit
GCGAATGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG2860.10158161869954216No Hit
GCGAATGGGGCTTTCCCAGGCGGCTGCCGAAGATGGCGGAGGGGCAGGTTCT2860.10158161869954216No Hit
GCGAATGGGGCTCTGCGTTCTGGAGTGGCGCTGCTTGGTCCGGCGGCTGGGT2860.10158161869954216No Hit
GCGAATGGGGGGTGTGCGAGGCGCGGGCTGTTGTGCTCCCGGCTCTGCTCGG2820.10016089675968844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAATC750.046.00000413
ACGCGAC253.4105382E-546.011
TCACGCT206.303296E-446.043
ATTTATA206.303296E-446.010
ACAACGG253.4105382E-546.026
TTTAGGT253.4105382E-546.045
TTTAGCG206.303296E-446.044
GTCATAG301.8563624E-646.044
GTTTGAC301.8563624E-646.040
ACTATTT206.303296E-446.044
AACGTAC253.4105382E-546.046
GAAATAC206.303296E-446.031
GAGGAAT405.5861165E-946.036
TAATAGC206.303296E-446.034
AGACTAA206.303296E-446.034
AACAACG600.046.038
AATCCTC301.8563624E-646.031
AACCGGT501.6370905E-1146.022
ACTATCT206.303296E-446.046
GACAGCG253.4105382E-546.040
Produced by FastQC (version 0.11.5)