SRR1527162_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2172424
Sequences flagged as poor quality	0
Sequence length	50
%GC	59

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	35397	1.629378058795152	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	17531	0.8069787481633419	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	12168	0.5601116540785777	No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	8401	0.38671088148538224	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7976	0.36714748133881786	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	7796	0.35886180598262585	No Hit
GTCCCGGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGT	5469	0.25174643623896625	No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5185	0.23867348178808556	No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4537	0.20884505050579447	No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4478	0.20612919025015372	No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4263	0.19623241135247999	No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	4246	0.19544987534661742	No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	4103	0.18886736659142048	No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	3426	0.1577040209461873	No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3412	0.1570595795295946	No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGC	3216	0.14803739969729665	No Hit
GTCCCGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGG	3178	0.14628820156654504	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC	2772	0.12759940048535645	No Hit
GTCCCGGGGTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTC	2770	0.1275073374258432	No Hit
GTCCCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	2675	0.12313434209896411	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGA	2639	0.12147720702772571	No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	2620	0.1206026079623499	No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	2566	0.1181169053554923	No Hit
GTCCCGGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCAT	2395	0.11024551376710992	No Hit
GTCCCGGGGGTCCAGGTCTGGTTAATTGCCAGGGTAGCAAAGAATTGCAT	2340	0.1077137796304957	No Hit
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2220	0.10218999605970105	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	40	8.3182385E-9	44.000004	22
ACGTTAG	30	2.528761E-6	44.000004	24
CCGTACG	30	2.528761E-6	44.000004	25
GCGATAT	40	8.3182385E-9	44.000004	17
ATTGCGC	20	7.85803E-4	44.000004	12
CTTACGA	30	2.528761E-6	44.000004	35
CGTATAC	25	4.4441524E-5	44.0	38
GCGTAAG	35	1.4468787E-7	44.0	31
CGCTTAT	25	4.4441524E-5	44.0	39
GTCCCGG	221375	0.0	42.82534	1
TCCCGGG	220010	0.0	42.797054	2
CCCGGGG	212625	0.0	42.74286	3
CCGGGGG	116610	0.0	42.718983	4
CGGGGGG	54030	0.0	42.546364	5
CGGGGGA	28495	0.0	42.49447	5
CCGGGGA	67145	0.0	42.394516	4
CGGGGAG	25870	0.0	42.358715	5
CGGGGAA	14295	0.0	42.21476	5
CGGGGGC	16755	0.0	42.188	5
CGGGGGT	18275	0.0	41.85718	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content