##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2172424
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38776132099443	33.0	31.0	34.0	31.0	34.0
2	32.31037403379819	33.0	31.0	34.0	31.0	34.0
3	32.80699808140584	34.0	31.0	34.0	31.0	34.0
4	36.47410542325071	37.0	37.0	37.0	35.0	37.0
5	36.530542380308816	37.0	37.0	37.0	35.0	37.0
6	36.17368478713179	37.0	37.0	37.0	35.0	37.0
7	36.56367357385115	37.0	37.0	37.0	35.0	37.0
8	36.63056198974049	37.0	37.0	37.0	35.0	37.0
9	38.65421805319772	39.0	39.0	39.0	38.0	39.0
10	38.02749647398482	39.0	38.0	39.0	35.0	39.0
11	36.58728728830099	38.0	35.0	39.0	33.0	39.0
12	36.300995569925576	39.0	35.0	39.0	33.0	39.0
13	36.24373695006131	38.0	35.0	39.0	33.0	39.0
14	36.904230021395456	39.0	35.0	40.0	33.0	40.0
15	36.97649584059097	39.0	35.0	40.0	33.0	40.0
16	36.89159160458548	39.0	35.0	40.0	33.0	40.0
17	36.74513538793532	39.0	35.0	40.0	31.0	40.0
18	36.78551332520723	39.0	35.0	40.0	32.0	40.0
19	36.5880044595346	39.0	35.0	40.0	31.0	40.0
20	36.33834693411599	38.0	35.0	40.0	31.0	40.0
21	36.52900446689965	39.0	35.0	40.0	31.0	40.0
22	36.41395464237184	38.0	35.0	40.0	31.0	40.0
23	36.4780793252146	38.0	35.0	40.0	31.0	40.0
24	36.58936054840123	38.0	35.0	40.0	32.0	40.0
25	36.514490265252086	38.0	35.0	40.0	32.0	40.0
26	36.3700249122639	38.0	35.0	40.0	31.0	40.0
27	36.225280147890096	38.0	35.0	40.0	31.0	40.0
28	36.011680040360446	38.0	35.0	40.0	31.0	40.0
29	36.29165577253796	38.0	35.0	40.0	31.0	40.0
30	36.30899492916668	38.0	35.0	40.0	32.0	40.0
31	36.23064144016085	38.0	35.0	40.0	31.0	40.0
32	35.504620184641674	37.0	35.0	40.0	30.0	40.0
33	35.6992539209657	37.0	35.0	40.0	30.0	40.0
34	35.3979609873579	37.0	34.0	40.0	29.0	40.0
35	35.46524527440315	37.0	34.0	40.0	30.0	40.0
36	35.35752551067379	36.0	34.0	40.0	30.0	40.0
37	35.34184026690922	36.0	34.0	40.0	30.0	40.0
38	35.179553807175765	36.0	34.0	40.0	30.0	40.0
39	35.13584640935655	35.0	34.0	40.0	30.0	40.0
40	34.973888614745555	35.0	34.0	40.0	29.0	40.0
41	34.82413654056482	35.0	34.0	39.0	29.0	40.0
42	34.72317420540374	35.0	34.0	39.0	29.0	40.0
43	34.66303999587557	35.0	34.0	39.0	29.0	40.0
44	34.53457198042371	35.0	34.0	39.0	29.0	40.0
45	34.43294080713525	35.0	34.0	39.0	28.0	40.0
46	34.125066745718144	35.0	34.0	39.0	27.0	40.0
47	34.07181793241099	35.0	34.0	38.0	27.0	40.0
48	33.96603379450789	35.0	33.0	38.0	27.0	40.0
49	33.75063063195766	35.0	33.0	37.0	26.0	40.0
50	33.288043218082656	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	1.0
12	0.0
13	2.0
14	15.0
15	47.0
16	161.0
17	330.0
18	668.0
19	1278.0
20	2066.0
21	3285.0
22	5054.0
23	7158.0
24	9877.0
25	13687.0
26	18139.0
27	23383.0
28	30206.0
29	37562.0
30	48241.0
31	60334.0
32	79413.0
33	125019.0
34	288732.0
35	225428.0
36	232408.0
37	320414.0
38	476596.0
39	162919.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.20342437756166	0.5920575357296733	0.12635654918192765	0.07816153752674432
8	99.16084521253677	0.5754861850172894	0.09970429345284346	0.1639643089930879
9	96.1348705409257	1.8354151859857928	1.299331990440172	0.7303822826483228
10	54.18748826195991	30.835509090306495	6.187051883057819	8.789950764675773
11	40.720503916362546	24.186208585432677	19.62347129289678	15.469816205307987
12	35.364275113882	16.499403431374354	23.772108943742108	24.364212511001533
13	26.14636921705892	15.651502653257376	26.903956133793404	31.298171995890307
14	23.19699101096287	18.478897305498375	30.5762595147172	27.747852168821556
15	27.413341042080187	20.248533435461955	27.667158897158195	24.670966625299666
16	35.53325685961856	20.453373742878924	22.74961057325826	21.263758824244256
17	32.71322725213863	21.451981749419083	23.655833299576877	22.178957698865414
18	32.86089639959787	21.666074394317132	22.90832728785909	22.564701918225907
19	30.391719111922903	24.425572540167114	23.10023273541445	22.082475612495536
20	33.494290249048994	24.4115789551211	21.58639381630842	20.507736979521493
21	30.85755819305992	22.90749872032347	25.224495770623044	21.010447315993563
22	28.964511531818836	20.89007486568	27.52565797468634	22.61975562781483
23	27.700992071529313	21.27660162104635	27.750614060606953	23.27179224681738
24	31.261807087382575	21.712197987133266	23.678388749157623	23.347606176326536
25	27.561148284128695	23.52933865580568	22.042566276196542	26.86694678386908
26	25.406228250102192	23.037629854945443	25.17312458341466	26.38301731153771
27	24.03310771746215	21.04386620659687	25.694799910146454	29.228226165794524
28	24.593403497659757	25.917592514168504	22.518118010112207	26.970885978059535
29	29.353201769083753	23.098667663402725	22.299652369887276	25.248478197626245
30	28.032695274955532	21.566784384632097	25.54340220877692	24.857118131635445
31	32.30773550651254	21.088424727401282	22.98211583005896	23.621723936027223
32	33.245627925303715	20.50341001572437	23.134848445791427	23.116113613180485
33	35.00140856481055	20.809933972373717	22.529856050200145	21.658801412615585
34	28.269297337904568	21.496356144104467	29.034019141751333	21.20032737623963
35	25.96127643590754	27.911079973338538	26.047861743379748	20.07978184737418
36	25.538200645914426	29.287054460823487	23.690310915364588	21.484433977897503
37	30.24497059505879	27.343419148379873	20.74940251074376	21.662207745817575
38	28.891505525624833	28.121213906677518	21.410369246519096	21.576911321178553
39	30.965410067279684	24.352198281735056	21.08069143040217	23.60170022058309
40	27.68060010384713	23.188889461725704	22.215598796551685	26.914911637875477
41	24.756493207587468	26.015639672550112	22.074742315496422	27.153124804365998
42	23.367169576473103	22.629192091414936	23.827162653331023	30.17647567878094
43	23.620895368491603	21.38385508537928	25.89908783920634	29.096161706922775
44	25.013671364337714	21.50666720676995	24.072050391636253	29.407611037256075
45	27.349955625605315	22.230466980663074	23.617304909170585	26.802272484561023
46	25.745204435229958	24.85665781633788	23.608743044635855	25.789394703796315
47	25.374006179272556	23.153675341461888	24.667330134448893	26.804988344816667
48	25.339896815722895	22.842179979598825	27.823389909152173	23.994533295526104
49	28.896384867779034	21.377364639683595	24.86116890625403	24.865081586283342
50	23.980907962718142	22.299146023059954	25.760348808519883	27.959597205702018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	23.5
10	47.0
11	25.5
12	4.0
13	19.5
14	35.0
15	361.0
16	687.0
17	1745.5
18	2804.0
19	3387.5
20	3971.0
21	4182.0
22	4393.0
23	4783.5
24	5174.0
25	5137.5
26	5101.0
27	5291.0
28	5481.0
29	6834.5
30	8188.0
31	9756.0
32	11324.0
33	14844.0
34	18364.0
35	19381.0
36	20398.0
37	22733.0
38	25068.0
39	31204.5
40	37341.0
41	39058.5
42	40776.0
43	43482.0
44	46188.0
45	53580.5
46	60973.0
47	67412.5
48	73852.0
49	75792.0
50	77732.0
51	84018.0
52	90304.0
53	96209.0
54	102114.0
55	105495.0
56	108876.0
57	118927.0
58	128978.0
59	131981.0
60	134984.0
61	152292.0
62	169600.0
63	177645.5
64	185691.0
65	204087.5
66	222484.0
67	212770.5
68	203057.0
69	163627.5
70	124198.0
71	101891.5
72	79585.0
73	71047.0
74	62509.0
75	51685.5
76	40862.0
77	34417.5
78	27973.0
79	27581.5
80	27190.0
81	18883.5
82	10577.0
83	7021.0
84	3465.0
85	2319.0
86	1173.0
87	868.5
88	564.0
89	400.5
90	237.0
91	163.0
92	89.0
93	48.5
94	8.0
95	4.5
96	1.0
97	2.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2172424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	7.311619092717009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.00241483824082	4.825845164967101
2	10.948684047318427	1.6010521464099907
3	4.848744199367505	1.0635651199138891
4	2.5345165844896673	0.7412567939985022
5	1.5339035832620962	0.5607659362883088
6	0.9220850261406144	0.4045160689342907
7	0.6089016889812917	0.31164400503202727
8	0.4432257263501614	0.2592558146532164
9	0.3360950949507227	0.2211659381889216
>10	4.746067475515811	9.333348569096104
>50	3.5442503771950626	18.93884345765529
>100	3.336653876881135	42.949618729436715
>500	0.1267915334195824	6.2147508206862465
>1k	0.0624103402842504	7.7746949219407115
>5k	0.0032847547518026527	1.672871450128996
>10k+	0.001970852851081592	3.1268050626696855
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	35397	1.629378058795152	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	17531	0.8069787481633419	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	12168	0.5601116540785777	No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	8401	0.38671088148538224	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	7976	0.36714748133881786	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	7796	0.35886180598262585	No Hit
GTCCCGGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGT	5469	0.25174643623896625	No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	5185	0.23867348178808556	No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4537	0.20884505050579447	No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4478	0.20612919025015372	No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4263	0.19623241135247999	No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	4246	0.19544987534661742	No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	4103	0.18886736659142048	No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	3426	0.1577040209461873	No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3412	0.1570595795295946	No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGC	3216	0.14803739969729665	No Hit
GTCCCGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGG	3178	0.14628820156654504	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGC	2772	0.12759940048535645	No Hit
GTCCCGGGGTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTC	2770	0.1275073374258432	No Hit
GTCCCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	2675	0.12313434209896411	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGA	2639	0.12147720702772571	No Hit
GTCCCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	2620	0.1206026079623499	No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGC	2566	0.1181169053554923	No Hit
GTCCCGGGGATGGCTACAGAAAACACGGGGTATGTAGGCTCCTGAGTCAT	2395	0.11024551376710992	No Hit
GTCCCGGGGGTCCAGGTCTGGTTAATTGCCAGGGTAGCAAAGAATTGCAT	2340	0.1077137796304957	No Hit
GTCCCGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2220	0.10218999605970105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	9.206305951324419E-5	0.0	0.0	0.0	0.0
8	1.3809458926986628E-4	0.0	0.0	0.0	0.0
9	5.523783570794651E-4	0.0	0.0	0.0	0.0
10	0.0045571214459055875	0.0	0.0	0.0	0.0
11	0.014453900343579337	0.0	0.0	0.0	0.0
12	0.019701494735834258	0.0	0.0	0.0	0.0
13	0.023015764878311046	0.0	0.0	0.0	0.0
14	0.04566327751856912	0.0	0.0	0.0	0.0
15	0.052798164630845544	0.0	0.0	0.0	0.0
16	0.0606235246894713	0.0	0.0	0.0	0.0
17	0.06577905602221297	0.0	0.0	0.0	0.0
18	0.06881713698615004	0.0	0.0	0.0	0.0
19	0.0756298033901301	0.0	0.0	0.0	0.0
20	0.08258056438338005	0.0	0.0	0.0	0.0
21	0.08621705523415318	0.0	0.0	0.0	0.0
22	0.08994560914443958	0.0	0.0	0.0	0.0
23	0.09565351883426071	0.0	0.0	0.0	0.0
24	0.10223602758945767	0.0	0.0	0.0	0.0
25	0.10614870761877056	0.0	0.0	0.0	0.0
26	0.11116614436224236	0.0	0.0	0.0	0.0
27	0.11738040087938635	0.0	0.0	0.0	0.0
28	0.14596598085824866	0.0	0.0	0.0	0.0
29	0.15319293103003834	0.0	0.0	0.0	0.0
30	0.16410240358235778	0.0	0.0	0.0	0.0
31	0.17160554293268718	0.0	0.0	0.0	0.0
32	0.17768170486056128	0.0	0.0	0.0	0.0
33	0.18320548843135595	0.0	0.0	0.0	0.0
34	0.1933324249778128	0.0	0.0	0.0	0.0
35	0.233748108104127	0.0	0.0	0.0	0.0
36	0.2435528239422875	0.0	0.0	0.0	0.0
37	0.25197659388774934	0.0	0.0	0.0	0.0
38	0.259571796297592	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	40	8.3182385E-9	44.000004	22
ACGTTAG	30	2.528761E-6	44.000004	24
CCGTACG	30	2.528761E-6	44.000004	25
GCGATAT	40	8.3182385E-9	44.000004	17
ATTGCGC	20	7.85803E-4	44.000004	12
CTTACGA	30	2.528761E-6	44.000004	35
CGTATAC	25	4.4441524E-5	44.0	38
GCGTAAG	35	1.4468787E-7	44.0	31
CGCTTAT	25	4.4441524E-5	44.0	39
GTCCCGG	221375	0.0	42.82534	1
TCCCGGG	220010	0.0	42.797054	2
CCCGGGG	212625	0.0	42.74286	3
CCGGGGG	116610	0.0	42.718983	4
CGGGGGG	54030	0.0	42.546364	5
CGGGGGA	28495	0.0	42.49447	5
CCGGGGA	67145	0.0	42.394516	4
CGGGGAG	25870	0.0	42.358715	5
CGGGGAA	14295	0.0	42.21476	5
CGGGGGC	16755	0.0	42.188	5
CGGGGGT	18275	0.0	41.85718	5
>>END_MODULE