FastQCFastQC Report
Fri 17 Jun 2016
SRR1527161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319218
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC331681.4301372272895434No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT152060.6556520344357452No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC117890.5083178899094436No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA85700.3695211058210138No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC77140.3326121132209219No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT73880.31855565108584016No Hit
GTCCCGGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTA53350.23003443402043275No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC48310.20830297108766835No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG44330.19114201424790597No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG43160.18609721035279997No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC42490.18320830555816658No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC42120.18161294022381683No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG41330.17820661964507004No Hit
GTCCCGGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC37820.16307220795975197No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32780.14134074502698754No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC32410.13974537969263778No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTC31050.13388133413935213No Hit
GTCCCGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTG25860.11150310147644595No Hit
GTCCCGGGGTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTG25600.11038203394420015No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA25330.10921784842994492No Hit
GTCCCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT25070.10809678089769914No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG24350.1049922861930185No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC24110.10395745462479164No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATGCG206.312789E-446.00000436
TAAGACG206.312789E-446.00000441
ATCGTAC301.8622286E-646.025
GTCCCGG2360100.044.8529741
TCCCGGG2346000.044.80492
CCGGGGG1254850.044.7554664
CCCGGGG2265400.044.7339553
CGGGGGG585750.044.606065
CGGGGGA309400.044.5058175
CCGGGGA700200.044.2097974
CGGGGAG270450.044.1205375
CGGGGGC185350.044.0269785
CGGGGGT185300.043.728555
CGGGGAT171950.043.3114285
CCGGGGT133600.043.1594284
CCGGGGC197050.042.918554
ATTCGTA2850.042.77192735
CGGGGAC111950.042.651185
CGGGGAA160550.042.361265
CGTAAGG1250.042.3219
Produced by FastQC (version 0.11.5)