##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2319218
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33920787092891	33.0	31.0	34.0	31.0	34.0
2	32.23724591651151	33.0	31.0	34.0	31.0	34.0
3	32.76099831926106	34.0	31.0	34.0	31.0	34.0
4	36.411444719728806	37.0	37.0	37.0	35.0	37.0
5	36.48644499999569	37.0	37.0	37.0	35.0	37.0
6	36.21445590712042	37.0	37.0	37.0	35.0	37.0
7	36.56202564830042	37.0	37.0	37.0	35.0	37.0
8	36.592221602281455	37.0	37.0	37.0	35.0	37.0
9	38.61943379190745	39.0	39.0	39.0	38.0	39.0
10	37.921899105646816	39.0	38.0	39.0	35.0	39.0
11	36.50989730159045	38.0	35.0	39.0	33.0	39.0
12	36.261430361440794	39.0	35.0	39.0	33.0	39.0
13	36.248078015951926	38.0	35.0	39.0	33.0	39.0
14	36.911313641063494	39.0	35.0	40.0	33.0	40.0
15	36.888672819890154	39.0	35.0	40.0	33.0	40.0
16	31.98600476539937	38.0	33.0	40.0	3.0	40.0
17	33.26472285054704	38.0	32.0	40.0	16.0	40.0
18	35.22745554751645	38.0	33.0	40.0	27.0	40.0
19	36.12014135799222	38.0	34.0	40.0	30.0	40.0
20	36.47059181155027	38.0	35.0	40.0	31.0	40.0
21	36.68715230737257	39.0	35.0	40.0	32.0	40.0
22	36.76072193299638	39.0	35.0	40.0	32.0	40.0
23	36.73919786755708	38.0	35.0	40.0	32.0	40.0
24	36.7517766764487	38.0	35.0	40.0	32.0	40.0
25	36.67710366166527	38.0	35.0	40.0	32.0	40.0
26	36.543433174457945	38.0	35.0	40.0	32.0	40.0
27	36.40115806276081	38.0	35.0	40.0	32.0	40.0
28	36.27195244259056	38.0	35.0	40.0	31.0	40.0
29	36.05299846758692	38.0	35.0	40.0	31.0	40.0
30	35.95456399527772	37.0	35.0	40.0	31.0	40.0
31	35.84551732523635	37.0	34.0	40.0	31.0	40.0
32	35.73960360776779	37.0	34.0	40.0	31.0	40.0
33	35.42274508045384	37.0	34.0	40.0	30.0	40.0
34	34.9449185027022	36.0	34.0	40.0	27.0	40.0
35	34.95813675126702	36.0	34.0	40.0	28.0	40.0
36	34.998745266723525	35.0	34.0	40.0	29.0	40.0
37	34.76308738548942	35.0	34.0	39.0	28.0	40.0
38	34.78903190644433	35.0	34.0	39.0	29.0	40.0
39	34.43027046185395	35.0	33.0	39.0	28.0	40.0
40	34.321483362064285	35.0	33.0	39.0	27.0	40.0
41	34.24266455331064	35.0	33.0	39.0	27.0	40.0
42	34.191367952473634	35.0	33.0	39.0	27.0	40.0
43	34.14269809910064	35.0	33.0	38.0	27.0	40.0
44	34.48349529884642	35.0	34.0	39.0	29.0	40.0
45	34.56604036360532	35.0	34.0	39.0	29.0	40.0
46	34.49941359544467	35.0	34.0	38.0	29.0	40.0
47	34.25506011077872	35.0	34.0	38.0	29.0	40.0
48	34.207274607216746	35.0	34.0	38.0	28.0	40.0
49	34.19786540118264	35.0	34.0	37.0	29.0	40.0
50	33.717642325990916	35.0	33.0	37.0	27.0	40.0
51	33.79954363927841	35.0	33.0	37.0	27.0	40.0
52	33.47135715573094	35.0	33.0	36.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	15.0
15	40.0
16	132.0
17	303.0
18	609.0
19	1235.0
20	2040.0
21	3473.0
22	5276.0
23	7649.0
24	11197.0
25	15620.0
26	21120.0
27	26941.0
28	34089.0
29	43582.0
30	56140.0
31	76162.0
32	110967.0
33	174789.0
34	305709.0
35	261563.0
36	293221.0
37	360594.0
38	390274.0
39	116476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.19261578687298	0.6010646692117774	0.1250852658094237	0.0812342781058098
8	99.11771985212256	0.5978308205610685	0.11745338299375048	0.1669959443226122
9	96.16086111784232	1.8376452752608856	1.2680567329160088	0.7334368739807987
10	54.343921097542356	30.723718080835866	6.108007095495119	8.824353726126652
11	40.86485185954921	24.169827933380994	19.45944710674029	15.505873100329506
12	35.50834807249685	16.454641176465515	23.5709622812517	24.466048469785935
13	26.242595564539428	15.602371144066666	26.714306287722845	31.440727003671064
14	23.266118148444864	18.36955387548734	30.611826917521334	27.752501058546457
15	27.617024359072754	20.14489366674457	27.72231847113984	24.515763503042837
16	33.14561201232485	28.174884810311063	20.219013477818816	18.460489699545278
17	32.95438376211292	21.345686347725827	23.56850455627716	22.131425333884096
18	33.041783911646085	21.565113758171936	22.828556867012935	22.56454546316905
19	30.50769699096851	24.41215099227412	22.980504635614246	22.099647381143125
20	33.49046963243645	24.450137934424447	21.462622314935466	20.596770118203636
21	31.00213089067091	22.92151923622531	25.041199231810037	21.035150641293747
22	28.738393717192608	20.824001883393453	27.688945153064527	22.748659246349415
23	27.92411062694408	21.126258937279722	27.624871831798476	23.32475860397772
24	31.374670255232584	21.64764157573803	23.576912562769003	23.400775606260385
25	27.656434194629398	23.41547883812561	21.91406758657444	27.01401938067055
26	25.43318480625797	22.94251769346392	25.147873119301416	26.47642438097669
27	24.128262198723878	20.89221453093241	25.558701251887488	29.42082201845622
28	24.622308036588194	25.934129521243797	22.4511882884662	26.99237415370181
29	29.723596488126603	23.0724321732584	22.02233683940018	25.181634499214823
30	28.13939871111728	21.492330604539976	25.450518235025772	24.917752449316968
31	32.41795294793331	21.10008632219998	22.856066139534963	23.625894590331743
32	33.466970332241296	20.007778483954507	23.287935847341647	23.23731533646255
33	35.383392160633456	20.747424347344666	22.332182658120107	21.53700083390177
34	28.427728656814494	21.345643229743818	28.961486156109515	21.26514195733217
35	26.007904388461974	27.926352762008573	26.053997511230076	20.011745338299374
36	25.550163891449618	29.36351822036566	23.631068748172876	21.455249140011848
37	30.413570436241873	27.266302693407862	20.64501914007221	21.67510773027805
38	28.877664799083142	28.17350503488676	21.27855164973711	21.670278516292992
39	31.27480900889869	24.307848593793253	20.973103865182143	23.444238532125915
40	27.74991397962589	23.116412514908042	22.14673221749745	26.98694128796862
41	24.87416016950541	26.007861270479964	21.79419959658816	27.323778963426467
42	23.278967307083683	22.639829459757557	23.795003315772817	30.286199917385947
43	23.622402033788976	21.29303929169229	25.71940197083672	29.365156703682015
44	24.956903576981553	21.379189019747173	23.992181847502046	29.67172555576923
45	27.330807194494007	22.197525200304586	23.611363830394556	26.860303774806855
46	25.42615657519043	24.827851456827258	23.716097408695518	26.02989455928679
47	25.627431315210558	23.16949075076168	24.53365746557676	26.669420468451005
48	25.424474973892064	22.792035936250926	27.81346988510783	23.97001920474919
49	29.130206819712505	21.20676020969137	24.892054132039334	24.77097883855679
50	24.03361822821313	22.420876347113552	25.53011402981522	28.015391394858096
51	23.545307081956075	22.967138061191317	27.463826168993172	26.023728687859442
52	23.4528190105458	22.6094744004229	25.447586212249128	28.490120376782173
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	23.5
10	47.0
11	26.5
12	6.0
13	23.5
14	385.5
15	730.0
16	1807.0
17	2884.0
18	3461.0
19	4038.0
20	4254.5
21	4471.0
22	4940.0
23	5409.0
24	5132.0
25	4855.0
26	4787.5
27	4720.0
28	5686.5
29	6653.0
30	7949.5
31	9246.0
32	12162.0
33	15078.0
34	17571.5
35	20065.0
36	21593.5
37	23122.0
38	27501.0
39	33393.0
40	34906.0
41	39528.0
42	44150.0
43	48615.5
44	53081.0
45	58401.5
46	63722.0
47	70848.0
48	77974.0
49	79278.5
50	80583.0
51	87604.5
52	94626.0
53	96483.0
54	98340.0
55	105511.5
56	112683.0
57	121587.0
58	130491.0
59	142756.0
60	155021.0
61	161111.5
62	167202.0
63	183824.5
64	213238.5
65	226030.0
66	217640.5
67	209251.0
68	172358.5
69	135466.0
70	117084.0
71	98702.0
72	82304.0
73	65906.0
74	58803.0
75	51700.0
76	43321.5
77	34943.0
78	32167.0
79	29391.0
80	21967.0
81	14543.0
82	9460.5
83	4378.0
84	3035.5
85	1693.0
86	1097.5
87	502.0
88	319.5
89	109.0
90	81.0
91	43.5
92	6.0
93	7.5
94	9.0
95	8.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2319218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.011703086648797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.46976046514888	5.485593912607371
2	10.250904173348289	1.6425440121311106
3	4.450384046580482	1.0696546680828423
4	2.3028760324115276	0.7379983606816387
5	1.3286621650389356	0.5322423384377957
6	0.8023677649510573	0.38569993794515295
7	0.5481700097897158	0.30742427516083964
8	0.4114280591289494	0.26369915610058625
9	0.3099375737234817	0.22348150344619716
>10	4.600482728064446	10.046085725497441
>50	3.3920471762413813	19.78282193600085
>100	2.961920967523764	41.273123655020726
>500	0.11279966856859701	6.099248675010209
>1k	0.05392072068938428	7.7226628729927045
>5k	0.0024791135949142197	1.440336931573935
>10k+	0.0018593351961856649	2.987382039310602
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	33168	1.4301372272895434	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	15206	0.6556520344357452	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	11789	0.5083178899094436	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8570	0.3695211058210138	No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7714	0.3326121132209219	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	7388	0.31855565108584016	No Hit
GTCCCGGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTA	5335	0.23003443402043275	No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4831	0.20830297108766835	No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4433	0.19114201424790597	No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4316	0.18609721035279997	No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4249	0.18320830555816658	No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4212	0.18161294022381683	No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4133	0.17820661964507004	No Hit
GTCCCGGGGGGGCTGATGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3782	0.16307220795975197	No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3278	0.14134074502698754	No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3241	0.13974537969263778	No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTC	3105	0.13388133413935213	No Hit
GTCCCGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTG	2586	0.11150310147644595	No Hit
GTCCCGGGGTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTG	2560	0.11038203394420015	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	2533	0.10921784842994492	No Hit
GTCCCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2507	0.10809678089769914	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG	2435	0.1049922861930185	No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	2411	0.10395745462479164	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.2935394602835957E-4	0.0	0.0	0.0	0.0
8	2.1558991004726592E-4	0.0	0.0	0.0	0.0
9	5.605337661228915E-4	0.0	0.0	0.0	0.0
10	0.004053090308888599	0.0	0.0	0.0	0.0
11	0.012331742854703611	0.0	0.0	0.0	0.0
12	0.01664354105564893	0.0	0.0	0.0	0.0
13	0.019187501994206668	0.0	0.0	0.0	0.0
14	0.03876306582649842	0.0	0.0	0.0	0.0
15	0.04561882496600147	0.0	0.0	0.0	0.0
16	0.05493230908004336	0.0	0.0	0.0	0.0
17	0.05907163535295087	0.0	0.0	0.0	0.0
18	0.062477955931697665	0.0	0.0	0.0	0.0
19	0.06967865892727634	0.0	0.0	0.0	0.0
20	0.07567205842659035	0.0	0.0	0.0	0.0
21	0.07998385662753565	0.0	0.0	0.0	0.0
22	0.08377823904436754	0.0	0.0	0.0	0.0
23	0.09011658239975716	0.0	0.0	0.0	0.0
24	0.09636868979112787	0.0	0.0	0.0	0.0
25	0.09994748229791249	0.0	0.0	0.0	0.0
26	0.10607023574325485	0.0	0.0	0.0	0.0
27	0.11253793304467281	0.0	0.0	0.0	0.0
28	0.14168568888306318	0.0	0.0	0.0	0.0
29	0.14789467829242442	0.0	0.0	0.0	0.0
30	0.15901911765086335	0.0	0.0	0.0	0.0
31	0.16565928688031914	0.0	0.0	0.0	0.0
32	0.17225633812776547	0.0	0.0	0.0	0.0
33	0.17764608587894712	0.0	0.0	0.0	0.0
34	0.1852348507126109	0.0	0.0	0.0	0.0
35	0.2205915959603625	0.0	0.0	0.0	0.0
36	0.23020690594847057	0.0	0.0	0.0	0.0
37	0.23779567078213432	0.0	0.0	0.0	0.0
38	0.244996373777713	0.0	0.0	0.0	0.0
39	0.25094665529501753	0.0	0.0	0.0	0.0
40	0.2564226390102181	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATGCG	20	6.312789E-4	46.000004	36
TAAGACG	20	6.312789E-4	46.000004	41
ATCGTAC	30	1.8622286E-6	46.0	25
GTCCCGG	236010	0.0	44.852974	1
TCCCGGG	234600	0.0	44.8049	2
CCGGGGG	125485	0.0	44.755466	4
CCCGGGG	226540	0.0	44.733955	3
CGGGGGG	58575	0.0	44.60606	5
CGGGGGA	30940	0.0	44.505817	5
CCGGGGA	70020	0.0	44.209797	4
CGGGGAG	27045	0.0	44.120537	5
CGGGGGC	18535	0.0	44.026978	5
CGGGGGT	18530	0.0	43.72855	5
CGGGGAT	17195	0.0	43.311428	5
CCGGGGT	13360	0.0	43.159428	4
CCGGGGC	19705	0.0	42.91855	4
ATTCGTA	285	0.0	42.771927	35
CGGGGAC	11195	0.0	42.65118	5
CGGGGAA	16055	0.0	42.36126	5
CGTAAGG	125	0.0	42.32	19
>>END_MODULE