FastQCFastQC Report
Fri 17 Jun 2016
SRR1527160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250769
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC312631.3889919400880322No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT147160.6538209829618232No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC110170.4894771520311502No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA83540.3711620339537287No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC78290.34783667271052693No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT69200.3074504758151547No Hit
GTCCCGGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTA51000.2265892235053886No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC47560.21130555823365257No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC42160.1873137580977879No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG42010.186647319205125No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC41580.184736861046158No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG41330.1836261295583865No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG40670.18069379843066968No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT33460.14866030232333927No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC32800.14572797119562247No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTC30530.13564252928665713No Hit
GTCCCGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTG25860.11489406509508529No Hit
GTCCCGGGGTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTG25410.11289474841709657No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG25220.1120505924863902No Hit
GTCCCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT24770.11005127580840149No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA24560.10911826135867342No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC23090.10258716021057691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG301.862205E-646.025
GTCCCGG2295000.044.7613071
TCCCGGG2280000.044.7198682
CCCGGGG2202800.044.680233
CCGGGGG1208350.044.6752174
CGGGGGA283800.044.4439775
CGGGGGG571450.044.422265
CCGGGGA694400.044.337274
CGGGGAG266600.044.334965
CGGGGGC178400.044.0532535
CGGGGGT186350.043.6055835
CGGGGAT172850.043.578255
CCGGGGT123400.043.297414
CGGGGAC108650.043.1845365
CGGGGAA158000.042.6810155
CCGGGGC194800.042.6468164
TGGCACG5750.042.415
CGGGGCA81050.041.8852585
TTCGTAC950.041.15789438
GTCCCGA15150.040.382841
Produced by FastQC (version 0.11.5)