##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2250769
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30575772102779	33.0	31.0	34.0	31.0	34.0
2	32.23637476791266	33.0	31.0	34.0	30.0	34.0
3	32.74348456016588	34.0	31.0	34.0	31.0	34.0
4	36.40715950859462	37.0	37.0	37.0	35.0	37.0
5	36.491682176180674	37.0	37.0	37.0	35.0	37.0
6	36.1719256840662	37.0	37.0	37.0	35.0	37.0
7	36.557339735885826	37.0	37.0	37.0	35.0	37.0
8	36.61414920855938	37.0	37.0	37.0	35.0	37.0
9	38.55895207371347	39.0	39.0	39.0	37.0	39.0
10	37.906778527694314	39.0	38.0	39.0	35.0	39.0
11	36.41768746592831	38.0	35.0	39.0	33.0	39.0
12	36.202514784946835	38.0	35.0	39.0	32.0	39.0
13	36.14631043878781	38.0	35.0	39.0	32.0	39.0
14	36.82718484215839	39.0	35.0	40.0	33.0	40.0
15	37.004254101598164	39.0	35.0	40.0	33.0	40.0
16	37.10979358610324	39.0	35.0	40.0	33.0	40.0
17	36.939688168799194	39.0	35.0	40.0	32.0	40.0
18	36.98385351850856	39.0	35.0	40.0	32.0	40.0
19	36.810555858908664	39.0	35.0	40.0	32.0	40.0
20	36.775530496465876	39.0	35.0	40.0	32.0	40.0
21	32.501920454742354	37.0	32.0	40.0	10.0	40.0
22	33.06325882398416	37.0	32.0	40.0	15.0	40.0
23	35.05890875518545	37.0	33.0	40.0	26.0	40.0
24	36.016240227229005	37.0	35.0	40.0	31.0	40.0
25	36.353184178385256	38.0	35.0	40.0	31.0	40.0
26	36.41976053517709	38.0	35.0	40.0	32.0	40.0
27	36.3674042071843	38.0	35.0	40.0	32.0	40.0
28	36.24510067448059	38.0	35.0	40.0	31.0	40.0
29	36.2040164939183	38.0	35.0	40.0	31.0	40.0
30	36.03935366090434	38.0	35.0	40.0	31.0	40.0
31	35.83364752224684	37.0	34.0	40.0	31.0	40.0
32	35.75785164981391	37.0	34.0	40.0	31.0	40.0
33	35.46423911116601	37.0	34.0	40.0	30.0	40.0
34	34.97258625829661	36.0	34.0	40.0	27.0	40.0
35	34.98325016916441	36.0	34.0	40.0	28.0	40.0
36	34.974889471109655	35.0	34.0	40.0	29.0	40.0
37	34.934256691824	35.0	34.0	39.0	29.0	40.0
38	34.89541219023365	35.0	34.0	39.0	29.0	40.0
39	34.746112550865945	35.0	34.0	39.0	29.0	40.0
40	30.016168251828596	35.0	28.0	38.0	8.0	40.0
41	30.709760086441566	35.0	28.0	38.0	12.0	40.0
42	32.39627700577003	35.0	30.0	38.0	22.0	40.0
43	33.33350690364049	35.0	32.0	38.0	25.0	40.0
44	34.157161841130744	35.0	33.0	38.0	28.0	40.0
45	34.432883605558814	35.0	34.0	39.0	29.0	40.0
46	34.471854730538766	35.0	34.0	38.0	29.0	40.0
47	34.46580079963781	35.0	34.0	38.0	29.0	40.0
48	34.340317909123506	35.0	34.0	38.0	29.0	40.0
49	34.26982600169098	35.0	34.0	37.0	29.0	40.0
50	34.16425897104501	35.0	34.0	37.0	29.0	40.0
51	34.01026760187296	35.0	34.0	37.0	29.0	40.0
52	33.622741383056194	35.0	33.0	36.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	18.0
15	47.0
16	142.0
17	305.0
18	658.0
19	1302.0
20	2219.0
21	3533.0
22	5632.0
23	8394.0
24	11668.0
25	16372.0
26	22082.0
27	28079.0
28	35870.0
29	46667.0
30	62652.0
31	90970.0
32	122903.0
33	149833.0
34	317262.0
35	268309.0
36	264065.0
37	286032.0
38	384708.0
39	121041.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	0.0	100.0
6	100.0	0.0	0.0	0.0
7	99.20116191399472	0.5945079215148245	0.1221804636548664	0.08214970083558108
8	99.13500674658306	0.5956630822621068	0.09916610722824067	0.17016406392659575
9	96.13838648035404	1.82826402887191	1.3026658888584302	0.7306836019156119
10	54.37314979902425	30.73176323292173	6.080010876282729	8.815076091771301
11	40.887314513395204	24.167073564635018	19.449263784955274	15.496348137014504
12	35.484005688722384	16.474369426627078	23.566345546788675	24.47527933786186
13	26.392624032053046	15.509321480791677	26.72113397687635	31.376920510278932
14	23.237302450851242	18.40304358199353	30.571640181644582	27.78801378551064
15	27.53081280220227	20.092421745634493	27.796988495931835	24.579776956231402
16	35.494668711005	20.342647335199658	22.809981832875785	21.352702120919563
17	32.87778532581531	21.349281067937227	23.63276728975741	22.140166316490053
18	33.04372861008837	21.57618129625919	22.851389902739907	22.528700190912527
19	30.488113173764162	24.45924037517844	22.95939743261081	22.093249018446585
20	33.503882450842355	24.460128960368657	21.421523043901885	20.614465544887103
21	35.24026677104581	21.810279064621913	23.808085147787267	19.141369016545013
22	28.82214922988543	20.80946556488027	27.626113563853067	22.742271641381233
23	27.875894860823124	21.19324550853508	27.615717117127524	23.31514251351427
24	31.449295774022122	21.67445881829721	23.538355113296834	23.337890294383833
25	27.62478068606774	23.486728313745214	21.898737720308038	26.989753279879007
26	25.503505690721706	22.948556693290158	25.14824933167286	26.39968828431527
27	24.138505550769537	20.961680207964477	25.566861814784193	29.332952426481796
28	24.628382566136285	25.995515310544974	22.41616087657152	26.95994124674722
29	29.544746706570063	23.099038595253443	22.03997833629306	25.316236361883426
30	28.130074654484755	21.48083610534888	25.515235015232573	24.87385422493379
31	32.533547423125164	21.02277043979191	22.812869734743995	23.630812402338933
32	33.41009228401493	20.097619968997265	23.248765199805042	23.243522547182764
33	35.35627156762867	20.802090307801464	22.333966746476428	21.507671378093445
34	28.436192252514587	21.31866930813424	28.9832052956123	21.261933143738872
35	25.983785986034107	27.908861371380183	26.08939433589142	20.01795830669429
36	25.606270567970324	29.402661934654333	23.574253954981607	21.416813542393733
37	30.335409808825336	27.35069658414524	20.661071838113994	21.652821768915423
38	28.876308497229168	28.173304323988823	21.282637178670935	21.66775000011107
39	31.017310083798026	24.303737966890427	20.98456127661257	23.694390672698976
40	28.219999475734735	27.475676091149293	20.419021232298828	23.88530320081714
41	24.833779032854995	25.94628769100694	21.829294787692564	27.390638488445507
42	23.28110970072895	22.62026889476441	23.84433942354813	30.25428198095851
43	23.62348157451964	21.29783198542365	25.67282559871759	29.405860841339116
44	24.859236998554714	21.44920247257715	23.99873110034837	29.69282942851976
45	27.3219064239822	22.187261331571566	23.591670224709866	26.899162019736366
46	25.418690234315473	24.80983166197864	23.76005711825603	26.011420985449863
47	25.418334800239386	23.179988706082234	24.56818091949907	26.83349557417931
48	25.427576086217645	22.78074738011764	27.760245498316355	24.031431035348362
49	29.098943516638094	21.205463554900568	24.87598682939031	24.819606099071027
50	23.928266294764143	22.39567898793701	25.623420262141515	28.052634455157328
51	23.61561759558622	22.955443228514344	27.438710947236256	25.99022822866318
52	23.413064601476208	22.6717624065375	25.39847492123803	28.516698070748266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	15.5
10	31.0
11	18.5
12	6.0
13	22.5
14	346.5
15	654.0
16	1622.5
17	2591.0
18	3249.0
19	3907.0
20	4167.0
21	4427.0
22	4815.5
23	5204.0
24	4985.0
25	4766.0
26	4683.5
27	4601.0
28	5470.0
29	6339.0
30	7515.0
31	8691.0
32	11313.5
33	13936.0
34	16763.0
35	19590.0
36	20927.0
37	22264.0
38	26562.5
39	32057.5
40	33254.0
41	37497.5
42	41741.0
43	46246.0
44	50751.0
45	56081.5
46	61412.0
47	68350.5
48	75289.0
49	76261.0
50	77233.0
51	84594.0
52	91955.0
53	93781.0
54	95607.0
55	102813.0
56	110019.0
57	118352.0
58	126685.0
59	138050.5
60	149416.0
61	155648.5
62	161881.0
63	176549.5
64	204253.5
65	217289.0
66	212818.5
67	208348.0
68	170881.0
69	133414.0
70	115330.0
71	97246.0
72	81163.0
73	65080.0
74	58078.0
75	51076.0
76	42271.0
77	33466.0
78	31075.5
79	28685.0
80	21706.5
81	14728.0
82	9651.0
83	4574.0
84	3121.0
85	1668.0
86	1113.0
87	558.0
88	357.0
89	120.5
90	85.0
91	45.0
92	5.0
93	7.0
94	9.0
95	8.0
96	7.0
97	7.0
98	7.0
99	3.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2250769.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.246585510436152
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.41770666204471	5.642124684164888
2	10.331587815505138	1.7040064475828671
3	4.4112075119863485	1.0913219985562117
4	2.306875022952352	0.760953685546637
5	1.28934622367902	0.5316351943063488
6	0.8020959020613313	0.39687314663515133
7	0.5487602948214094	0.3167779087183834
8	0.38937544271440744	0.25688143072066355
9	0.2902475116356847	0.21541958315054865
>10	4.801298983399429	10.837513788911423
>50	3.40573465842278	20.366612598556728
>100	2.8432561953246585	40.23654442753423
>500	0.10896839206716126	6.0716393177281365
>1k	0.049130257695020677	7.146188060698809
>5k	0.002519500394616445	1.4649537486344995
>10k+	0.001889625295962334	2.9605539785544774
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	31263	1.3889919400880322	No Hit
GTCCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	14716	0.6538209829618232	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	11017	0.4894771520311502	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	8354	0.3711620339537287	No Hit
GTCCCGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7829	0.34783667271052693	No Hit
GTCCCGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	6920	0.3074504758151547	No Hit
GTCCCGGGGAGCTTTGGCACGTCCAGAGTTCTTTGTCATTACCTTTATGTTA	5100	0.2265892235053886	No Hit
GTCCCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4756	0.21130555823365257	No Hit
GTCCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4216	0.1873137580977879	No Hit
GTCCCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	4201	0.186647319205125	No Hit
GTCCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4158	0.184736861046158	No Hit
GTCCCGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4133	0.1836261295583865	No Hit
GTCCCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4067	0.18069379843066968	No Hit
GTCCCGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3346	0.14866030232333927	No Hit
GTCCCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3280	0.14572797119562247	No Hit
GTCCCGGGGGGAGCTGGAGAGATGGCTCAGTGGTTAAGAGCATCCTCTGCTC	3053	0.13564252928665713	No Hit
GTCCCGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTG	2586	0.11489406509508529	No Hit
GTCCCGGGGTGTCCCTTCCCCAGCACTCCTGAACCATTCTCCTCCACCTCTG	2541	0.11289474841709657	No Hit
GTCCCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTCCCGGGGAAG	2522	0.1120505924863902	No Hit
GTCCCGGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	2477	0.11005127580840149	No Hit
GTCCCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA	2456	0.10911826135867342	No Hit
GTCCCGGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC	2309	0.10258716021057691	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.7771703804344205E-4	0.0	0.0	0.0	0.0
8	2.2214629755430256E-4	0.0	0.0	0.0	0.0
9	6.664388926629077E-4	0.0	0.0	0.0	0.0
10	0.004709501508151214	0.0	0.0	0.0	0.0
11	0.013417636372279874	0.0	0.0	0.0	0.0
12	0.017993850101898506	0.0	0.0	0.0	0.0
13	0.021370473824723905	0.0	0.0	0.0	0.0
14	0.041319211345100276	0.0	0.0	0.0	0.0
15	0.048294605088305374	0.0	0.0	0.0	0.0
16	0.05691388143341231	0.0	0.0	0.0	0.0
17	0.06100137330841148	0.0	0.0	0.0	0.0
18	0.06433356777172602	0.0	0.0	0.0	0.0
19	0.07090909817933337	0.0	0.0	0.0	0.0
20	0.07708476525134299	0.0	0.0	0.0	0.0
21	0.07983937934101634	0.0	0.0	0.0	0.0
22	0.08388244195650464	0.0	0.0	0.0	0.0
23	0.09050240162362286	0.0	0.0	0.0	0.0
24	0.0974333661073171	0.0	0.0	0.0	0.0
25	0.1018318627988923	0.0	0.0	0.0	0.0
26	0.10742994949726072	0.0	0.0	0.0	0.0
27	0.11493849435459615	0.0	0.0	0.0	0.0
28	0.14475052748638353	0.0	0.0	0.0	0.0
29	0.1505263312227954	0.0	0.0	0.0	0.0
30	0.1610116364673585	0.0	0.0	0.0	0.0
31	0.16776488391300928	0.0	0.0	0.0	0.0
32	0.1737184046874646	0.0	0.0	0.0	0.0
33	0.17936092064534387	0.0	0.0	0.0	0.0
34	0.18695832402170104	0.0	0.0	0.0	0.0
35	0.2196138297621835	0.0	0.0	0.0	0.0
36	0.22854411092386648	0.0	0.0	0.0	0.0
37	0.23623037281924533	0.0	0.0	0.0	0.0
38	0.24387220545511334	0.0	0.0	0.0	0.0
39	0.24938143363446005	0.0	0.0	0.0	0.0
40	0.2558236762635348	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	30	1.862205E-6	46.0	25
GTCCCGG	229500	0.0	44.761307	1
TCCCGGG	228000	0.0	44.719868	2
CCCGGGG	220280	0.0	44.68023	3
CCGGGGG	120835	0.0	44.675217	4
CGGGGGA	28380	0.0	44.443977	5
CGGGGGG	57145	0.0	44.42226	5
CCGGGGA	69440	0.0	44.33727	4
CGGGGAG	26660	0.0	44.33496	5
CGGGGGC	17840	0.0	44.053253	5
CGGGGGT	18635	0.0	43.605583	5
CGGGGAT	17285	0.0	43.57825	5
CCGGGGT	12340	0.0	43.29741	4
CGGGGAC	10865	0.0	43.184536	5
CGGGGAA	15800	0.0	42.681015	5
CCGGGGC	19480	0.0	42.646816	4
TGGCACG	575	0.0	42.4	15
CGGGGCA	8105	0.0	41.885258	5
TTCGTAC	95	0.0	41.157894	38
GTCCCGA	1515	0.0	40.38284	1
>>END_MODULE