FastQCFastQC Report
Fri 17 Jun 2016
SRR1527159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401664
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGGTGGGGGGTGTCTCCTGTATTCTTTTAGTTGATGATGCTGT29320.7299633524537923No Hit
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29020.7224944231994901No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGA26800.6672243467176545No Hit
TATGTAGGGATGTGTGTCTGTCTGTATGGTGTATGTCTGTCTGTCTGGTG23910.5952736615678776No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA18630.4638205066921606No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18540.46157982791586993No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAGTGTATACATGTCAGCATGT15770.3926167144678139No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAA15640.3893801784576163No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC13600.338591459528362No Hit
TATGTAGGGATTTATAAGTTCTGAGATTGTGCTGTGAGTTTTTTTTTCAC13130.3268901370299554No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA13090.32589427979604846No Hit
TATGTAGGGCTTTGGGGGGCTGCCACTGGCTGTTGCGCCTTGTGCCGGCA12210.3039854206500956No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12180.30323852772466536No Hit
TATGTAGGGCTAAAGATGAGGCAGAAAATGGTTGAATCCCCTGTTTTTTT11790.29352891969407263No Hit
TATGTAGGGATGCAGATTCCTCAGCAGGCTTTTATCCCAACAGTGCCCAT11420.28431724028043337No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA11200.2788400254939452No Hit
TATGTAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG10890.27112213193116635No Hit
TATGTAGGGAGTCTTGATAGCCTCTGGAAAGCCCTGGCCTGTCGTGTTCC10120.25195188017845765No Hit
TATGTAGGGATTTGTGAGATGACAGCCTTTTTGTATCTCAAACTGGATGC9900.24647466539196938No Hit
TATGTAGGGATCCTCACAGGAGTGAAAGGGAAAGAGCTCTGGTCTCTCTT9830.24473191523263224No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA9720.24199330783938816No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA9710.24174434353091143No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCA9340.23253266411727216No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGG9210.22929612810707456No Hit
TATGTAGGGATTCCCACCACTCCAAATTCTTCCTTGCCTACTTTCTCTCT8880.22108030592734224No Hit
TATGTAGGGGATTGTTTTCCCCCTCCCAGGGAAATCTATGTACCCTCCCA7710.19195148183556407No Hit
TATGTAGGGATTTTGGATAGTGTGTCTTGGTACTAATAACTAAATGGATA7700.19170251752708733No Hit
TATGTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGA7400.18423358827278521No Hit
TATGTAGGGAGCTCTGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTT7240.18025015933715743No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAA7020.17477294455066922No Hit
TATGTAGGGATTCCAGCCCCAGGGCCTTTGCACTTGCTGTTTTTGTTGGC6560.16332058636073932No Hit
TATGTAGGGATAGGCCCTGTTCATGTTTCTTCCTCTCATAGACATGCCCT6350.15809233588272786No Hit
TATGTAGGGAGACTGTGGCTTCCTTAAGAACCGAGCTTTAGGACAGCAAG6100.15186822817080942No Hit
TATGTAGGGACGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAA6060.15087237093690248No Hit
TATGTAGGGATTTAGGGTGCATCTTCTTTCCTCAATTAAACCCTGTACAA6020.14987651370299554No Hit
TATGTAGGGAGGGATTGAACTTAGGGCTTGCAAAGTACAGACAAGCACAG5780.14390137029955385No Hit
TATGTAGGGATTTCAGGCTGTCATGATGTTGGCCTTCGCGATGCCTTCGC5540.13792622689611217No Hit
TATGTAGGGGCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCG5540.13792622689611217No Hit
TATGTAGGGATGTGTAAGGGTGTTTTGCAGGCATGTATACTATGTGGGTG5500.13693036966220523No Hit
TATGTAGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA5130.12771869024856597No Hit
TATGTAGGGAGCCCTAGCTGCCCTGGAACTTACTTTATAGACCAGGCTAG5090.12672283301465903No Hit
TATGTAGGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAA4960.12348629700446145No Hit
TATGTAGGGGTGTGCAGCTGAGGGAGAATTTCTCGCTTTCCTGTTGTGGC4790.11925390376035692No Hit
TATGTAGGGGTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCG4640.11551943913320585No Hit
TATGTAGGGGTGTGTGTGTGTGCAAGGGGGGTATTTTAATTGGGAGAGAT4580.11402565328234544No Hit
TATGTAGGGAAGCCATCTTTCCAGCCCCCTCCTTCCTTTTTCCCCTCTAT4580.11402565328234544No Hit
TATGTAGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGA4540.11302979604843849No Hit
TATGTAGGGATGGTACACTGGGTCTCCAGGCTAGCTCCGACATGGTACAC4530.11278083173996176No Hit
TATGTAGGGGGGAGGGAGCCTGCTAATCTTGGGCTGGCTTGCCTTGGCTT4510.11228290312300829No Hit
TATGTAGGGAGCCTCTTATTTCCTTAAAGGAAACTTCTAGAAGTGAGATT4450.11078911727214785No Hit
TATGTAGGGGAGAACACCTTTGATTGAGTTTGGAAGCATCTCTTGGCCTG4410.10979326003824091No Hit
TATGTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330.10780154557042702No Hit
TATGTAGGGCCCCCACACCAGCTATGCATTCACCAGTGGGTAACCGCACA4240.10556086679413638No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA4240.10556086679413638No Hit
TATGTAGGGGCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4230.10531190248565965No Hit
TATGTAGGGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA4150.10332018801784576No Hit
TATGTAGGGAGCAGCAAATCCATGTCCTATAAGTTGGTTTCATTCTGATA4140.10307122370936903No Hit
TATGTAGGGATCCAGTGTCAGGAAATTCAGGGTAGTTTCAATCTGCTTTA4090.10182640216698535No Hit
TATGTAGGGATGTGGGGCTGTGGGATCAAATTTCGGTTGTCATGCTCAGG4050.10083054493307839No Hit
TATGTAGGGGACTACCATCAGCAATAGAAGGCCCTACACCAGTTTCAGCA4040.10058158062460165No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAC207.850357E-444.00000443
TCGCCTA408.289135E-944.00000419
ATCCTTA207.850357E-444.00000424
TCGCCCA207.850357E-444.00000419
GTTGACC207.850357E-444.00000410
CTAGATC207.850357E-444.00000420
CTTCGGC207.850357E-444.00000434
ATCCTAT207.850357E-444.00000441
GTCATAG408.289135E-944.00000419
GTCATAC207.850357E-444.00000444
CGTGGCG207.850357E-444.00000425
CAGAACG207.850357E-444.00000417
CGGAACC207.850357E-444.00000417
CTCACTG207.850357E-444.00000418
CGGCGAA207.850357E-444.00000431
AGCCGCT207.850357E-444.00000427
GTCGCGT408.289135E-944.00000410
CTCACCC207.850357E-444.00000435
ACCGGTG207.850357E-444.00000438
ACAAACG207.850357E-444.00000432
Produced by FastQC (version 0.11.5)