FastQCFastQC Report
Fri 17 Jun 2016
SRR1527158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432446
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28320.6548794531571571No Hit
TATGTAGGGGTGGGGGGTGTCTCCTGTATTCTTTTAGTTGATGATGCTGTTA27470.6352238198526521No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG25790.5963750387331598No Hit
TATGTAGGGATGTGTGTCTGTCTGTATGGTGTATGTCTGTCTGTCTGGTGTG20810.4812161518432359No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA18410.425717893101104No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18120.41901185350309633No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA15770.36466980848475883No Hit
TATGTAGGGATTTATAAGTTCTGAGATTGTGCTGTGAGTTTTTTTTTCACAG14180.3279022120680964No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC13980.3232773571729187No Hit
TATGTAGGGCTAAAGATGAGGCAGAAAATGGTTGAATCCCCTGTTTTTTTAT12910.2985343834837182No Hit
TATGTAGGGATGCAGATTCCTCAGCAGGCTTTTATCCCAACAGTGCCCATGA12140.2807286921372842No Hit
TATGTAGGGCTTTGGGGGGCTGCCACTGGCTGTTGCGCCTTGTGCCGGCAGC11830.27356016704975883No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAGTGTATACATGTCAGCATGTGT11520.2663916419622334No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11370.2629230007908502No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA11220.25945435961946695No Hit
TATGTAGGGATTTGTGAGATGACAGCCTTTTTGTATCTCAAACTGGATGCTT10770.2490484361053172No Hit
TATGTAGGGAGTCTTGATAGCCTCTGGAAAGCCCTGGCCTGTCGTGTTCCTT10010.23147398750364206No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC9640.2229180059475634No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA9640.2229180059475634No Hit
TATGTAGGGATCCTCACAGGAGTGAAAGGGAAAGAGCTCTGGTCTCTCTTCT9460.2187556365419035No Hit
TATGTAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA9030.2088121985172715No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA8850.20464982911161164No Hit
TATGTAGGGGTGTGCAGCTGAGGGAGAATTTCTCGCTTTCCTGTTGTGGCCA8620.19933124598215732No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC8550.19771254676884514No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA8530.19725006127932734No Hit
TATGTAGGGATTTTGGATAGTGTGTCTTGGTACTAATAACTAAATGGATACA7830.18106306914620554No Hit
TATGTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG7490.1732008158244035No Hit
TATGTAGGGGATTGTTTTCCCCCTCCCAGGGAAATCTATGTACCCTCCCAGC7410.17135087386633244No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA7280.16834471818446695No Hit
TATGTAGGGAGCTCTGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTTTG7080.16371986328928928No Hit
TATGTAGGGATAGGCCCTGTTCATGTTTCTTCCTCTCATAGACATGCCCTTC6790.1570138236912817No Hit
TATGTAGGGACGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAA6360.1470703856666497No Hit
TATGTAGGGATTCCCACCACTCCAAATTCTTCCTTGCCTACTTTCTCTCTTC6010.1389768896000888No Hit
TATGTAGGGATTCCAGCCCCAGGGCCTTTGCACTTGCTGTTTTTGTTGGCTG5570.12880220883069793No Hit
TATGTAGGGATGTGTAAGGGTGTTTTGCAGGCATGTATACTATGTGGGTGTC5550.12833972334118018No Hit
TATGTAGGGAGACTGTGGCTTCCTTAAGAACCGAGCTTTAGGACAGCAAGTG5500.12718350961738575No Hit
TATGTAGGGATTTCAGGCTGTCATGATGTTGGCCTTCGCGATGCCTTCGCTA5420.1253335676593147No Hit
TATGTAGGGGGGCTCGCGGACAGCTCCGCGGCCATCTGATTCTTTCGGTTTT5410.1251023249145558No Hit
TATGTAGGGATTTAGGGTGCATCTTCTTTCCTCAATTAAACCCTGTACAAAC5370.12417735393552026No Hit
TATGTAGGGAGCCCTAGCTGCCCTGGAACTTACTTTATAGACCAGGCTAGCC5250.12140244099841367No Hit
TATGTAGGGGTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCGAC4980.11515888688992382No Hit
TATGTAGGGAGGGATTGAACTTAGGGCTTGCAAAGTACAGACAAGCACAGTA4700.10868409003667509No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC4520.10452172063101521No Hit
TATGTAGGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA4490.10382799239673855No Hit
TATGTAGGGGCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGA4480.10359674965197967No Hit
TATGTAGGGATGGTACACTGGGTCTCCAGGCTAGCTCCGACATGGTACACTG4400.1017468076939086No Hit
TATGTAGGGGAGAACACCTTTGATTGAGTTTGGAAGCATCTCTTGGCCTGAA4370.10105307945963195No Hit
TATGTAGGGAAGCCATCTTTCCAGCCCCCTCCTTCCTTTTTCCCCTCTATTT4360.10082183671487306No Hit
TATGTAGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA4340.1003593512253553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC750.046.00000430
GAACCGA750.046.00000428
CTTGTTG206.307067E-446.023
TGATCAC600.046.021
TCTGCCG206.307067E-446.025
ATCCTTA206.307067E-446.010
TCGCCAA301.8586907E-646.026
GTCATGC453.092282E-1046.039
GTCATAG405.5970304E-946.019
ATCCTAC206.307067E-446.030
AGACTTA301.8586907E-646.046
AACCGTG206.307067E-446.033
CGGGTCC253.4135963E-546.041
CTATGGT206.307067E-446.013
CGGGTAA253.4135963E-546.030
CGTGGCG206.307067E-446.025
CCTTATA206.307067E-446.036
ACTATAC206.307067E-446.016
AACCGAC206.307067E-446.020
CGGCGAT206.307067E-446.022
Produced by FastQC (version 0.11.5)