##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	432446
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.690990320178706	31.0	31.0	34.0	28.0	34.0
2	31.393387844956365	31.0	31.0	34.0	28.0	34.0
3	32.30560116176355	34.0	31.0	34.0	30.0	34.0
4	36.03473266026278	37.0	35.0	37.0	35.0	37.0
5	35.03757925845077	35.0	35.0	37.0	33.0	37.0
6	35.79994958908164	37.0	35.0	37.0	35.0	37.0
7	36.33726754323083	37.0	35.0	37.0	35.0	37.0
8	36.54614448971663	37.0	37.0	37.0	35.0	37.0
9	38.584364290570385	39.0	39.0	39.0	37.0	39.0
10	37.75133311442354	39.0	38.0	39.0	35.0	39.0
11	37.408053259828975	39.0	37.0	39.0	34.0	39.0
12	37.18123650120477	39.0	37.0	39.0	34.0	39.0
13	36.99315752718258	39.0	37.0	39.0	33.0	39.0
14	37.8535493448893	40.0	38.0	40.0	33.0	40.0
15	37.76474056876466	40.0	37.0	40.0	33.0	40.0
16	32.79317186423276	39.0	34.0	40.0	3.0	40.0
17	34.56769168867327	39.0	34.0	40.0	17.0	40.0
18	36.4543480573297	39.0	34.0	40.0	30.0	40.0
19	37.28842445068286	39.0	36.0	40.0	32.0	40.0
20	37.68167354999237	39.0	37.0	40.0	33.0	40.0
21	37.8578643345065	40.0	37.0	40.0	34.0	40.0
22	37.95550889590839	40.0	38.0	40.0	34.0	40.0
23	37.867356849178854	40.0	38.0	40.0	34.0	40.0
24	37.77347691966165	40.0	37.0	40.0	34.0	40.0
25	37.6034325673032	39.0	37.0	40.0	33.0	40.0
26	37.25152042104679	39.0	36.0	40.0	33.0	40.0
27	37.199483866193695	39.0	36.0	40.0	33.0	40.0
28	37.117977735948536	39.0	35.0	40.0	33.0	40.0
29	36.901846704559645	39.0	35.0	40.0	32.0	40.0
30	36.810010960906105	39.0	35.0	40.0	32.0	40.0
31	36.73463276339705	39.0	35.0	40.0	31.0	40.0
32	36.37280955310027	39.0	35.0	40.0	30.0	40.0
33	36.12166143287254	39.0	35.0	40.0	30.0	40.0
34	35.95370520249927	39.0	35.0	40.0	30.0	40.0
35	36.051881622214104	39.0	35.0	40.0	30.0	40.0
36	35.807453416149066	39.0	35.0	40.0	29.0	40.0
37	35.33946666173349	39.0	35.0	40.0	25.0	40.0
38	35.16855283665475	39.0	35.0	40.0	24.0	40.0
39	34.714900819986774	38.0	35.0	40.0	22.0	40.0
40	34.63326519380455	38.0	35.0	40.0	22.0	40.0
41	34.53477659638429	38.0	34.0	40.0	21.0	40.0
42	34.3541760127276	38.0	34.0	40.0	21.0	40.0
43	34.06577931117411	38.0	34.0	40.0	18.0	40.0
44	34.23234114779649	38.0	34.0	40.0	20.0	40.0
45	34.12519250958501	38.0	34.0	40.0	18.0	40.0
46	33.99118271414235	37.0	34.0	40.0	18.0	40.0
47	33.62876983484643	37.0	33.0	40.0	15.0	40.0
48	33.54591324697188	37.0	33.0	40.0	15.0	40.0
49	33.32982615170449	36.0	33.0	40.0	15.0	40.0
50	32.82821670220097	35.0	33.0	40.0	12.0	40.0
51	32.82946078816777	35.0	33.0	40.0	12.0	40.0
52	32.411894201819415	35.0	32.0	40.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	2.0
14	3.0
15	18.0
16	41.0
17	120.0
18	285.0
19	508.0
20	706.0
21	1173.0
22	1658.0
23	2356.0
24	3207.0
25	4340.0
26	6241.0
27	8393.0
28	9782.0
29	9893.0
30	9694.0
31	10193.0
32	12492.0
33	16914.0
34	25439.0
35	35502.0
36	53602.0
37	83439.0
38	96294.0
39	40150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	99.58861915707395	0.23586759965406084	0.13967061783436543	0.03584262543762689
8	98.9224088094236	0.8491233587546191	0.1311146362782868	0.09735319554348983
9	96.07650434967603	2.4416921419090474	0.6585793370732993	0.8232241713416242
10	42.196944820856245	42.45593669498619	5.476984409614148	9.870134074543412
11	28.223639483311214	33.9399601337508	23.938942665673864	13.89745771726412
12	36.27689931228408	24.853507721195246	22.385916391873206	16.483676574647472
13	24.015252771444295	26.068457102158423	25.984747228555705	23.931542897841577
14	27.17634109229823	33.054531664069046	21.302312889933077	18.466814353699654
15	27.446201375431844	28.657219629734115	25.764141650055727	18.132437344778307
16	27.47464423303719	38.05423104850085	22.061020335486976	12.410104382974986
17	30.357316289201425	27.346304509696008	23.539355202730512	18.757023998372052
18	28.92014263052497	29.066519287957345	23.78655369687776	18.22678438463993
19	26.57765362611748	27.736873505593763	28.588771777285487	17.09670109100327
20	25.943123534499108	28.168603710058598	26.005096590094485	19.883176165347813
21	22.408578180859575	26.884975233902036	32.07799355295228	18.628453032286114
22	22.40510953968819	28.53466097501191	24.573704000037	24.4865254852629
23	22.371810584442915	31.838657312126834	27.8328392446687	17.956692858761556
24	23.57103545876248	30.66093801306984	29.089643562433228	16.67838296573445
25	26.002090434412622	30.11196773701225	24.652789018744535	19.233152809830592
26	26.436364309069806	29.89274961498083	25.079663125569436	18.591222950379933
27	25.32061806560819	29.64231372240696	26.277269300675695	18.759798911309158
28	27.701262122900893	33.56210948881479	23.46165764049153	15.274970747792787
29	31.902480309680282	30.49374950860917	22.472401178413026	15.13136900329752
30	28.45603844179389	30.85610688964634	24.147754864191135	16.540099804368637
31	31.241126059669877	29.90338678123974	22.040439731203435	16.81504742788695
32	26.549904496746414	32.08284965059221	25.405946638424222	15.961299214237155
33	28.282606383224728	34.07338719747668	21.794397450779982	15.849608968518613
34	24.345236168215223	35.737178746016845	23.615434065756187	16.302151020011745
35	25.59186580521036	40.64183736235275	20.292475823571035	13.473821008865846
36	27.401802768438138	37.233088061862055	20.222640514653854	15.142468655045949
37	24.65047659129695	36.47669304375575	21.067601504002813	17.80522886094449
38	24.195390869611465	37.4657182630895	21.897531714942446	16.441359152356593
39	24.576710155718864	37.09041128834583	20.45318953117846	17.879689024756846
40	23.707006192680705	36.81222626640089	22.594959833135235	16.88580770778317
41	20.987129028826722	40.10350425255408	22.16392335690468	16.745443361714525
42	21.569629502874346	41.63849359226354	22.30544391669711	14.486432988164996
43	24.285806782812188	36.60711395179976	22.168779454544612	16.938299810843436
44	24.3746039967996	38.86751178181784	21.41192195094879	15.345962270433766
45	22.553567381823395	36.85130629026514	23.45333290168021	17.141793426231253
46	23.501893878079574	39.575808309014306	20.37387326972616	16.54842454317996
47	23.975016533856248	39.38364558811967	19.913468964911228	16.72786891311285
48	23.63323975710262	38.59626404221568	20.93116828459507	16.83932791608663
49	24.619721306244017	39.010419798078836	19.046308672065415	17.323550223611733
50	21.458632985390082	42.186538897342096	19.982379302849374	16.372448814418448
51	20.93741183870356	41.52819080301355	21.461407898327188	16.072989459955693
52	21.343936583989677	41.16768336393445	19.608459784574258	17.879920267501607
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	22.0
4	39.0
5	99.0
6	159.0
7	1845.5
8	3532.0
9	3326.0
10	3120.0
11	3103.0
12	3086.0
13	2416.0
14	1751.5
15	1757.0
16	1836.5
17	1916.0
18	2342.0
19	2768.0
20	3158.0
21	3548.0
22	4303.0
23	5058.0
24	6438.5
25	7819.0
26	10208.5
27	12598.0
28	13870.5
29	15143.0
30	15917.5
31	16692.0
32	18040.0
33	19388.0
34	18776.0
35	18164.0
36	19114.0
37	20064.0
38	20163.0
39	19284.5
40	18307.0
41	18424.5
42	18542.0
43	21976.5
44	25411.0
45	26711.0
46	28011.0
47	31647.5
48	35284.0
49	35161.5
50	35039.0
51	32452.5
52	29866.0
53	25432.0
54	20998.0
55	18260.0
56	15522.0
57	14336.0
58	13150.0
59	11794.5
60	10439.0
61	8322.5
62	6206.0
63	5985.5
64	6112.0
65	6459.0
66	4475.0
67	2491.0
68	2278.0
69	2065.0
70	1533.5
71	1002.0
72	790.5
73	579.0
74	401.0
75	223.0
76	203.5
77	184.0
78	102.0
79	20.0
80	17.5
81	15.0
82	9.0
83	3.0
84	2.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	432446.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.20813889281232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.00016687172224	20.950312350155443
2	8.878330257389079	4.831256855586004
3	3.7064709380374095	3.0253852825288257
4	2.2149474775334	2.410583944360521
5	1.4591556368971998	1.9850454617464512
6	0.9821749232767484	1.603389103773063
7	0.7081885038688147	1.3487943822888961
8	0.524140149315271	1.1408702385495424
9	0.4215165961321099	1.0321813883869129
>10	2.959944465672744	16.37528762683848
>50	0.6250656302031303	12.201104147932519
>100	0.48597368535467256	22.913356989694506
>500	0.01950679714338127	3.845353094548313
>1k	0.014418067453803547	6.337079133610524
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2832	0.6548794531571571	No Hit
TATGTAGGGGTGGGGGGTGTCTCCTGTATTCTTTTAGTTGATGATGCTGTTA	2747	0.6352238198526521	No Hit
TATGTAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTATGTAGGGAAG	2579	0.5963750387331598	No Hit
TATGTAGGGATGTGTGTCTGTCTGTATGGTGTATGTCTGTCTGTCTGGTGTG	2081	0.4812161518432359	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAA	1841	0.425717893101104	No Hit
TATGTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1812	0.41901185350309633	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAA	1577	0.36466980848475883	No Hit
TATGTAGGGATTTATAAGTTCTGAGATTGTGCTGTGAGTTTTTTTTTCACAG	1418	0.3279022120680964	No Hit
TATGTAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	1398	0.3232773571729187	No Hit
TATGTAGGGCTAAAGATGAGGCAGAAAATGGTTGAATCCCCTGTTTTTTTAT	1291	0.2985343834837182	No Hit
TATGTAGGGATGCAGATTCCTCAGCAGGCTTTTATCCCAACAGTGCCCATGA	1214	0.2807286921372842	No Hit
TATGTAGGGCTTTGGGGGGCTGCCACTGGCTGTTGCGCCTTGTGCCGGCAGC	1183	0.27356016704975883	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAGTGTATACATGTCAGCATGTGT	1152	0.2663916419622334	No Hit
TATGTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1137	0.2629230007908502	No Hit
TATGTAGGGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAA	1122	0.25945435961946695	No Hit
TATGTAGGGATTTGTGAGATGACAGCCTTTTTGTATCTCAAACTGGATGCTT	1077	0.2490484361053172	No Hit
TATGTAGGGAGTCTTGATAGCCTCTGGAAAGCCCTGGCCTGTCGTGTTCCTT	1001	0.23147398750364206	No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAAC	964	0.2229180059475634	No Hit
TATGTAGGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAA	964	0.2229180059475634	No Hit
TATGTAGGGATCCTCACAGGAGTGAAAGGGAAAGAGCTCTGGTCTCTCTTCT	946	0.2187556365419035	No Hit
TATGTAGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGGAA	903	0.2088121985172715	No Hit
TATGTAGGGGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAAAA	885	0.20464982911161164	No Hit
TATGTAGGGGTGTGCAGCTGAGGGAGAATTTCTCGCTTTCCTGTTGTGGCCA	862	0.19933124598215732	No Hit
TATGTAGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCC	855	0.19771254676884514	No Hit
TATGTAGGGAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAA	853	0.19725006127932734	No Hit
TATGTAGGGATTTTGGATAGTGTGTCTTGGTACTAATAACTAAATGGATACA	783	0.18106306914620554	No Hit
TATGTAGGGGCCTTTTGTGACTGGCGGTGAACGAGTGCGCAGTGCCATGAAG	749	0.1732008158244035	No Hit
TATGTAGGGGATTGTTTTCCCCCTCCCAGGGAAATCTATGTACCCTCCCAGC	741	0.17135087386633244	No Hit
TATGTAGGGCAGAGTGCAGTGCTTATGTAGGGGAAAAAAAAAAAAAAAAAAA	728	0.16834471818446695	No Hit
TATGTAGGGAGCTCTGAACTTTGTCTCTGTAACTCCTTCCATGGGTATTTTG	708	0.16371986328928928	No Hit
TATGTAGGGATAGGCCCTGTTCATGTTTCTTCCTCTCATAGACATGCCCTTC	679	0.1570138236912817	No Hit
TATGTAGGGACGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAA	636	0.1470703856666497	No Hit
TATGTAGGGATTCCCACCACTCCAAATTCTTCCTTGCCTACTTTCTCTCTTC	601	0.1389768896000888	No Hit
TATGTAGGGATTCCAGCCCCAGGGCCTTTGCACTTGCTGTTTTTGTTGGCTG	557	0.12880220883069793	No Hit
TATGTAGGGATGTGTAAGGGTGTTTTGCAGGCATGTATACTATGTGGGTGTC	555	0.12833972334118018	No Hit
TATGTAGGGAGACTGTGGCTTCCTTAAGAACCGAGCTTTAGGACAGCAAGTG	550	0.12718350961738575	No Hit
TATGTAGGGATTTCAGGCTGTCATGATGTTGGCCTTCGCGATGCCTTCGCTA	542	0.1253335676593147	No Hit
TATGTAGGGGGGCTCGCGGACAGCTCCGCGGCCATCTGATTCTTTCGGTTTT	541	0.1251023249145558	No Hit
TATGTAGGGATTTAGGGTGCATCTTCTTTCCTCAATTAAACCCTGTACAAAC	537	0.12417735393552026	No Hit
TATGTAGGGAGCCCTAGCTGCCCTGGAACTTACTTTATAGACCAGGCTAGCC	525	0.12140244099841367	No Hit
TATGTAGGGGTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCGAC	498	0.11515888688992382	No Hit
TATGTAGGGAGGGATTGAACTTAGGGCTTGCAAAGTACAGACAAGCACAGTA	470	0.10868409003667509	No Hit
TATGTAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC	452	0.10452172063101521	No Hit
TATGTAGGGGCAGAGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAA	449	0.10382799239673855	No Hit
TATGTAGGGGCTCTATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGA	448	0.10359674965197967	No Hit
TATGTAGGGATGGTACACTGGGTCTCCAGGCTAGCTCCGACATGGTACACTG	440	0.1017468076939086	No Hit
TATGTAGGGGAGAACACCTTTGATTGAGTTTGGAAGCATCTCTTGGCCTGAA	437	0.10105307945963195	No Hit
TATGTAGGGAAGCCATCTTTCCAGCCCCCTCCTTCCTTTTTCCCCTCTATTT	436	0.10082183671487306	No Hit
TATGTAGGGTGCAGTGCTTATGTAGGGAAAAAAAAAAAAAAAAAAAAAAAAA	434	0.1003593512253553	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.12718350961738575	0.0	0.0	0.0	0.0
7	0.12718350961738575	0.0	0.0	0.0	0.0
8	0.13088339353352788	0.0	0.0	0.0	0.0
9	0.1389768896000888	0.0	0.0	0.0	0.0
10	0.21968060752093904	0.0	0.0	0.0	0.0
11	0.34732660262784254	0.0	0.0	0.0	0.0
12	0.42409919388779177	0.0	0.0	0.0	0.0
13	0.46988525735005066	0.0	0.0	0.0	0.0
14	0.5612261415298095	0.0	0.0	0.0	0.0
15	0.6305989649574745	0.0	0.0	0.0	0.0
16	0.7080652844517004	0.0	0.0	0.0	0.0
17	0.754313833403477	0.0	0.0	0.0	0.0
18	0.8003311396104947	0.0	0.0	0.0	0.0
19	0.8546731846288322	0.0	0.0	0.0	0.0
20	0.9016154618148856	0.0	0.0	0.0	0.0
21	0.9492514672352155	0.0	0.0	0.0	0.0
22	1.0019748130402408	0.0	0.0	0.0	0.0
23	1.0521544886529186	0.0	0.0	0.0	0.0
24	1.106496533671256	0.0	0.0	0.0	0.0
25	1.1576011802629693	0.0	0.0	0.0	0.0
26	1.2202679640926266	0.0	0.0	0.0	0.0
27	1.2857096608593905	0.0	0.0	0.0	0.0
28	1.3948562363855834	0.0	0.0	0.0	0.0
29	1.445035911998261	0.0	0.0	0.0	0.0
30	1.5090901522964717	0.0	0.0	0.0	0.0
31	1.5548762157587306	0.0	0.0	0.0	0.0
32	1.601587250200025	0.0	0.0	0.0	0.0
33	1.6519981685574614	0.0	0.0	0.0	0.0
34	1.7116587967052534	0.0	0.0	0.0	0.0
35	1.8455483459206468	0.0	0.0	0.0	0.0
36	1.8978092062361542	0.0	0.0	0.0	0.0
37	1.9503013092964208	0.0	0.0	0.0	0.0
38	2.0138930641051136	0.0	0.0	0.0	0.0
39	2.070316293826281	0.0	0.0	0.0	0.0
40	2.1278957372712433	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGC	75	0.0	46.000004	30
GAACCGA	75	0.0	46.000004	28
CTTGTTG	20	6.307067E-4	46.0	23
TGATCAC	60	0.0	46.0	21
TCTGCCG	20	6.307067E-4	46.0	25
ATCCTTA	20	6.307067E-4	46.0	10
TCGCCAA	30	1.8586907E-6	46.0	26
GTCATGC	45	3.092282E-10	46.0	39
GTCATAG	40	5.5970304E-9	46.0	19
ATCCTAC	20	6.307067E-4	46.0	30
AGACTTA	30	1.8586907E-6	46.0	46
AACCGTG	20	6.307067E-4	46.0	33
CGGGTCC	25	3.4135963E-5	46.0	41
CTATGGT	20	6.307067E-4	46.0	13
CGGGTAA	25	3.4135963E-5	46.0	30
CGTGGCG	20	6.307067E-4	46.0	25
CCTTATA	20	6.307067E-4	46.0	36
ACTATAC	20	6.307067E-4	46.0	16
AACCGAC	20	6.307067E-4	46.0	20
CGGCGAT	20	6.307067E-4	46.0	22
>>END_MODULE